LIVIVO - Search results -

Search results

Result 1 - 10 of total 11

Search options

Article: Widefield in vivo imaging system with two fluorescence and two reflectance channels, a single sCMOS detector, and shielded illumination.

Doran, Patrick R / Fomin-Thunemann, Natalie / Tang, Rockwell P / Balog, Dora / Zimmerman, Bernhard / Kilic, Kivilcim / Martin, Emily A / Kura, Sreekanth / Fisher, Harrison P / Chabbott, Grace / Herbert, Joel / Rauscher, Bradley C / Jiang, John X / Sakadzic, Sava / Boas, David A / Devor, Anna / Chen, Ichun Anderson / Thunemann, Martin

bioRxiv : the preprint server for biology

2024

Abstract: Significance: Widefield microscopy of the entire dorsal part of mouse cerebral cortex enables large-scale (mesoscopic) imaging of neuronal activity with fluorescent indicators as well as hemodynamics via oxy- and deoxyhemoglobin absorption. Versatile ... ...

Abstract	Significance: Widefield microscopy of the entire dorsal part of mouse cerebral cortex enables large-scale (mesoscopic) imaging of neuronal activity with fluorescent indicators as well as hemodynamics via oxy- and deoxyhemoglobin absorption. Versatile and cost-effective imaging systems are needed for large-scale, color-multiplexed imaging of multiple fluorescent and intrinsic contrasts. Aim: Develop a system for mesoscopic imaging of two fluorescent and two reflectance channels. Approach: Excitation of red and green fluorescence is achieved through epi-illumination. Hemoglobin absorption imaging is achieved using 525- and 625nm LEDs positioned around the objective lens. An aluminum hemisphere placed between objective and cranial window provides diffuse illumination of the brain. Signals are recorded sequentially by a single sCMOS detector. Results: We demonstrate performance of our imaging system by recording large-scale spontaneous and stimulus-evoked neuronal, cholinergic, and hemodynamic activity in awake head-fixed mice with a curved crystal skull window expressing the red calcium indicator jRGECO1a and the green acetylcholine sensor GRABACh3.0 . Shielding of illumination light through the aluminum hemisphere enables concurrent recording of pupil diameter changes. Conclusions: Our widefield microscope design with single camera can be used to acquire multiple aspects of brain physiology and is compatible with behavioral readouts of pupil diameter.
Language	English
Publishing date	2024-03-13
Publishing country	United States
Document type	Preprint
DOI	10.1101/2023.11.07.566086
Database	MEDical Literature Analysis and Retrieval System OnLINE

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article: Multi-Scale Label-free Human Brain Imaging with Integrated Serial Sectioning Polarization Sensitive Optical Coherence Tomography and Two-Photon Microscopy.

Chang, Shuaibin / Yang, Jiarui / Novoseltseva, Anna / Fu, Xinlei / Li, Chenglin / Chen, Shih-Chi / Augustinack, Jean C / Magnain, Caroline / Fischl, Bruce / Mckee, Ann C / Boas, David A / Chen, Ichun Anderson / Wang, Hui

bioRxiv : the preprint server for biology

2023

Abstract: The study of neurodegenerative processes in the human brain requires a comprehensive understanding of cytoarchitectonic, myeloarchitectonic, and vascular structures. Recent computational advances have enabled volumetric reconstruction of the human brain ... ...

Abstract	The study of neurodegenerative processes in the human brain requires a comprehensive understanding of cytoarchitectonic, myeloarchitectonic, and vascular structures. Recent computational advances have enabled volumetric reconstruction of the human brain using thousands of stained slices, however, tissue distortions and loss resulting from standard histological processing have hindered deformation-free reconstruction of the human brain. The development of a multi-scale and volumetric human brain imaging technique that can measure intact brain structure would be a major technical advance. Here, we describe the development of integrated serial sectioning Polarization Sensitive Optical Coherence Tomography (PSOCT) and Two Photon Microscopy (2PM) to provide label-free multi-contrast imaging, including scattering, birefringence and autofluorescence of human brain tissue. We demonstrate that high-throughput reconstruction of 4×4×2cm
Language	English
Publishing date	2023-05-24
Publishing country	United States
Document type	Preprint
DOI	10.1101/2023.05.22.541785
Database	MEDical Literature Analysis and Retrieval System OnLINE

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article ; Online: High-speed low-light in vivo two-photon voltage imaging of large neuronal populations.

Platisa, Jelena / Ye, Xin / Ahrens, Allison M / Liu, Chang / Chen, Ichun Anderson / Davison, Ian G / Tian, Lei / Pieribone, Vincent A / Chen, Jerry L

Nature methods

2023 Volume 20, Issue 7, Page(s) 1095–1103

Abstract: Monitoring spiking activity across large neuronal populations at behaviorally relevant timescales is critical for understanding neural circuit function. Unlike calcium imaging, voltage imaging requires kilohertz sampling rates that reduce fluorescence ... ...

Abstract	Monitoring spiking activity across large neuronal populations at behaviorally relevant timescales is critical for understanding neural circuit function. Unlike calcium imaging, voltage imaging requires kilohertz sampling rates that reduce fluorescence detection to near shot-noise levels. High-photon flux excitation can overcome photon-limited shot noise, but photobleaching and photodamage restrict the number and duration of simultaneously imaged neurons. We investigated an alternative approach aimed at low two-photon flux, which is voltage imaging below the shot-noise limit. This framework involved developing positive-going voltage indicators with improved spike detection (SpikeyGi and SpikeyGi2); a two-photon microscope ('SMURF') for kilohertz frame rate imaging across a 0.4 mm × 0.4 mm field of view; and a self-supervised denoising algorithm (DeepVID) for inferring fluorescence from shot-noise-limited signals. Through these combined advances, we achieved simultaneous high-speed deep-tissue imaging of more than 100 densely labeled neurons over 1 hour in awake behaving mice. This demonstrates a scalable approach for voltage imaging across increasing neuronal populations.
MeSH term(s)	Mice ; Animals ; Neurons/physiology ; Microscopy ; Algorithms ; Calcium
Chemical Substances	Calcium (SY7Q814VUP)
Language	English
Publishing date	2023-03-27
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't
ZDB-ID	2169522-2
ISSN	1548-7105 ; 1548-7091
ISSN (online)	1548-7105
ISSN	1548-7091
DOI	10.1038/s41592-023-01820-3
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 6022: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds.

Clough, Mitchell / Chen, Ichun Anderson / Park, Seong-Wook / Ahrens, Allison M / Stirman, Jeffrey N / Smith, Spencer L / Chen, Jerry L

Nature communications

2021 Volume 12, Issue 1, Page(s) 6638

Abstract: Understanding brain function requires monitoring local and global brain dynamics. Two-photon imaging of the brain across mesoscopic scales has presented trade-offs between imaging area and acquisition speed. We describe a flexible cellular resolution two- ...

Abstract	Understanding brain function requires monitoring local and global brain dynamics. Two-photon imaging of the brain across mesoscopic scales has presented trade-offs between imaging area and acquisition speed. We describe a flexible cellular resolution two-photon microscope capable of simultaneous video rate acquisition of four independently targetable brain regions spanning an approximate five-millimeter field of view. With this system, we demonstrate the ability to measure calcium activity across mouse sensorimotor cortex at behaviorally relevant timescales.
MeSH term(s)	Animals ; Calcium/metabolism ; Equipment Design ; Mice ; Microscopy, Fluorescence, Multiphoton/instrumentation ; Neurons/cytology ; Neurons/physiology ; Optical Imaging/instrumentation ; Sensorimotor Cortex/cytology ; Sensorimotor Cortex/physiology
Chemical Substances	Calcium (SY7Q814VUP)
Language	English
Publishing date	2021-11-17
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-021-26737-3
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Multi-Scale Label-Free Human Brain Imaging with Integrated Serial Sectioning Polarization Sensitive Optical Coherence Tomography and Two-Photon Microscopy.

Chang, Shuaibin / Yang, Jiarui / Novoseltseva, Anna / Abdelhakeem, Ayman / Hyman, Mackenzie / Fu, Xinlei / Li, Chenglin / Chen, Shih-Chi / Augustinack, Jean C / Magnain, Caroline / Fischl, Bruce / Mckee, Ann C / Boas, David A / Chen, Ichun Anderson / Wang, Hui

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

2023 Volume 10, Issue 35, Page(s) e2303381

Abstract: The study of aging and neurodegenerative processes in the human brain requires a comprehensive understanding of cytoarchitectonic, myeloarchitectonic, and vascular structures. Recent computational advances have enabled volumetric reconstruction of the ... ...

Abstract	The study of aging and neurodegenerative processes in the human brain requires a comprehensive understanding of cytoarchitectonic, myeloarchitectonic, and vascular structures. Recent computational advances have enabled volumetric reconstruction of the human brain using thousands of stained slices, however, tissue distortions and loss resulting from standard histological processing have hindered deformation-free reconstruction. Here, the authors describe an integrated serial sectioning polarization-sensitive optical coherence tomography (PSOCT) and two photon microscopy (2PM) system to provide label-free multi-contrast imaging of intact brain structures, including scattering, birefringence, and autofluorescence of human brain tissue. The authors demonstrate high-throughput reconstruction of 4 × 4 × 2cm
MeSH term(s)	Humans ; Tomography, Optical Coherence/methods ; Microscopy/methods ; Lipofuscin ; Brain/diagnostic imaging ; Neuroimaging
Chemical Substances	Lipofuscin
Language	English
Publishing date	2023-10-26
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2808093-2
ISSN	2198-3844 ; 2198-3844
ISSN (online)	2198-3844
ISSN	2198-3844
DOI	10.1002/advs.202303381
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Scalable mapping of myelin and neuron density in the human brain with micrometer resolution.

Chang, Shuaibin / Varadarajan, Divya / Yang, Jiarui / Chen, Ichun Anderson / Kura, Sreekanth / Magnain, Caroline / Augustinack, Jean C / Fischl, Bruce / Greve, Douglas N / Boas, David A / Wang, Hui

Scientific reports

2022 Volume 12, Issue 1, Page(s) 363

Abstract: Optical coherence tomography (OCT) is an emerging 3D imaging technique that allows quantification of intrinsic optical properties such as scattering coefficient and back-scattering coefficient, and has proved useful in distinguishing delicate ... ...

Abstract	Optical coherence tomography (OCT) is an emerging 3D imaging technique that allows quantification of intrinsic optical properties such as scattering coefficient and back-scattering coefficient, and has proved useful in distinguishing delicate microstructures in the human brain. The origins of scattering in brain tissues are contributed by the myelin content, neuron size and density primarily; however, no quantitative relationships between them have been reported, which hampers the use of OCT in fundamental studies of architectonic areas in the human brain and the pathological evaluations of diseases. Here, we built a generalized linear model based on Mie scattering theory that quantitatively links tissue scattering to myelin content and neuron density in the human brain. We report a strong linear relationship between scattering coefficient and the myelin content that is retained across different regions of the brain. Neuronal cell body turns out to be a secondary contribution to the overall scattering. The optical property of OCT provides a label-free solution for quantifying volumetric myelin content and neuron cells in the human brain.
MeSH term(s)	Adult ; Aged ; Brain/cytology ; Brain/diagnostic imaging ; Brain/metabolism ; Cadaver ; Female ; Humans ; Imaging, Three-Dimensional ; Lasers ; Male ; Middle Aged ; Myelin Sheath ; Neuroimaging ; Neurons/chemistry ; Predictive Value of Tests ; Reproducibility of Results ; Scattering, Radiation ; Tomography, Optical Coherence
Language	English
Publishing date	2022-01-10
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-021-04093-y
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Volumetric Characterization of Microvasculature in Ex Vivo Human Brain Samples By Serial Sectioning Optical Coherence Tomography.

Yang, Jiarui / Chang, Shuaibin / Chen, Ichun Anderson / Kura, Sreekanth / Rosen, Grace A / Saltiel, Nicole A / Huber, Bertrand R / Varadarajan, Divya / Balbastre, Yael / Magnain, Caroline / Chen, Shih-Chi / Fischl, Bruce / McKee, Ann C / Boas, David A / Wang, Hui

IEEE transactions on bio-medical engineering

2022 Volume 69, Issue 12, Page(s) 3645–3656

Abstract: Objective: Serial sectioning optical coherence tomography (OCT) enables accurate volumetric reconstruction of several cubic centimeters of human brain samples. We aimed to identify anatomical features of the ex vivo human brain, such as intraparenchymal ...

Abstract	Objective: Serial sectioning optical coherence tomography (OCT) enables accurate volumetric reconstruction of several cubic centimeters of human brain samples. We aimed to identify anatomical features of the ex vivo human brain, such as intraparenchymal blood vessels and axonal fiber bundles, from the OCT data in 3D, using intrinsic optical contrast. Methods: We developed an automatic processing pipeline to enable characterization of the intraparenchymal microvascular network in human brain samples. Results: We demonstrated the automatic extraction of the vessels down to a 20 μm in diameter using a filtering strategy followed by a graphing representation and characterization of the geometrical properties of microvascular network in 3D. We also showed the ability to extend this processing strategy to extract axonal fiber bundles from the volumetric OCT image. Conclusion: This method provides a viable tool for quantitative characterization of volumetric microvascular network as well as the axonal bundle properties in normal and pathological tissues of the ex vivo human brain.
MeSH term(s)	Humans ; Tomography, Optical Coherence/methods ; Imaging, Three-Dimensional/methods ; Brain/diagnostic imaging ; Microvessels/diagnostic imaging ; Histological Techniques
Language	English
Publishing date	2022-11-23
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	160429-6
ISSN	1558-2531 ; 0018-9294
ISSN (online)	1558-2531
ISSN	0018-9294
DOI	10.1109/TBME.2022.3175072
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 425: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Awake chronic mouse model of targeted pial vessel occlusion via photothrombosis.

Sunil, Smrithi / Erdener, Sefik Evren / Lee, Blaire S / Postnov, Dmitry / Tang, Jianbo / Kura, Sreekanth / Cheng, Xiaojun / Chen, Ichun Anderson / Boas, David A / Kılıç, Kıvılcım

Neurophotonics

2020 Volume 7, Issue 1, Page(s) 15005

Abstract: Animal models of stroke are used extensively to study the mechanisms involved in the acute and chronic phases of recovery following stroke. A translatable animal model that closely mimics the mechanisms of a human stroke is essential in understanding ... ...

Abstract	Animal models of stroke are used extensively to study the mechanisms involved in the acute and chronic phases of recovery following stroke. A translatable animal model that closely mimics the mechanisms of a human stroke is essential in understanding recovery processes as well as developing therapies that improve functional outcomes. We describe a photothrombosis stroke model that is capable of targeting a single distal pial branch of the middle cerebral artery with minimal damage to the surrounding parenchyma in awake head-fixed mice. Mice are implanted with chronic cranial windows above one hemisphere of the brain that allow optical access to study recovery mechanisms for over a month following occlusion. Additionally, we study the effect of laser spot size used for occlusion and demonstrate that a spot size with small axial and lateral resolution has the advantage of minimizing unwanted photodamage while still monitoring macroscopic changes to cerebral blood flow during photothrombosis. We show that temporally guiding illumination using real-time feedback of blood flow dynamics also minimized unwanted photodamage to the vascular network. Finally, through quantifiable behavior deficits and chronic imaging we show that this model can be used to study recovery mechanisms or the effects of therapeutics longitudinally.
Language	English
Publishing date	2020-01-30
Publishing country	United States
Document type	Journal Article
ZDB-ID	2781943-7
ISSN	2329-4248 ; 2329-423X
ISSN (online)	2329-4248
ISSN	2329-423X
DOI	10.1117/1.NPh.7.1.015005
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Improving the characterization of

Yang, Jiarui / Chen, Ichun Anderson / Chang, Shuaibin / Tang, Jianbo / Lee, Blaire / Kılıç, Kıvılcım / Sunil, Smrithi / Wang, Hui / Varadarajan, Divya / Magnain, Caroline / Chen, Shih-Chi / Costantini, Irene / Pavone, Francesco / Fischl, Bruce / Boas, David A

Neurophotonics

2020 Volume 7, Issue 4, Page(s) 45005

Abstract: Significance: ...

Abstract	Significance:
Language	English
Publishing date	2020-10-21
Publishing country	United States
Document type	Journal Article
ZDB-ID	2781943-7
ISSN	2329-4248 ; 2329-423X
ISSN (online)	2329-4248
ISSN	2329-423X
DOI	10.1117/1.NPh.7.4.045005
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Baseline oxygen consumption decreases with cortical depth.

PLoS biology

2022 Volume 20, Issue 10, Page(s) e3001440

Abstract: The cerebral cortex is organized in cortical layers that differ in their cellular density, composition, and wiring. Cortical laminar architecture is also readily revealed by staining for cytochrome oxidase-the last enzyme in the respiratory electron ... ...

Abstract	The cerebral cortex is organized in cortical layers that differ in their cellular density, composition, and wiring. Cortical laminar architecture is also readily revealed by staining for cytochrome oxidase-the last enzyme in the respiratory electron transport chain located in the inner mitochondrial membrane. It has been hypothesized that a high-density band of cytochrome oxidase in cortical layer IV reflects higher oxygen consumption under baseline (unstimulated) conditions. Here, we tested the above hypothesis using direct measurements of the partial pressure of O2 (pO2) in cortical tissue by means of 2-photon phosphorescence lifetime microscopy (2PLM). We revisited our previously developed method for extraction of the cerebral metabolic rate of O2 (CMRO2) based on 2-photon pO2 measurements around diving arterioles and applied this method to estimate baseline CMRO2 in awake mice across cortical layers. To our surprise, our results revealed a decrease in baseline CMRO2 from layer I to layer IV. This decrease of CMRO2 with cortical depth was paralleled by an increase in tissue oxygenation. Higher baseline oxygenation and cytochrome density in layer IV may serve as an O2 reserve during surges of neuronal activity or certain metabolically active brain states rather than reflecting baseline energy needs. Our study provides to our knowledge the first quantification of microscopically resolved CMRO2 across cortical layers as a step towards better understanding of brain energy metabolism.
MeSH term(s)	Animals ; Mice ; Electron Transport Complex IV/metabolism ; Oxygen Consumption/physiology ; Oxygen/metabolism ; Cerebral Cortex/metabolism ; Brain/physiology ; Cerebrovascular Circulation
Chemical Substances	Electron Transport Complex IV (EC 1.9.3.1) ; Oxygen (S88TT14065)
Language	English
Publishing date	2022-10-27
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2126776-5
ISSN	1545-7885 ; 1544-9173
ISSN (online)	1545-7885
ISSN	1544-9173
DOI	10.1371/journal.pbio.3001440
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 6193: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito