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Article ; Online: Research on the Influencing Factors of Adoption Intention of Information Technology for Pandemic Prevention Among the Elderly in Public Health Emergency

CHEN Jinghao, LUO Qi

Nongye tushu qingbao xuebao, Vol 34, Iss 4, Pp 30-

2022 Volume 40

Abstract: Purpose/Significance] This paper takes the elderly aged 60 and above in Guangxi as the research object to explore the factors that affect their adoption of information technology for pandemic prevention and control. It is helpful to improve the elderly' ... ...

Abstract	[Purpose/Significance] This paper takes the elderly aged 60 and above in Guangxi as the research object to explore the factors that affect their adoption of information technology for pandemic prevention and control. It is helpful to improve the elderly's ability to apply information technology, and promote the construction and development of information technology to prevent a pandemic. [Method/Process] Based on the Unified Theory of Acceptance and Use of Technology (UTAUT) model, this paper added pandemic risk perception, technology anxiety, resistance to change as new variables to construct the model of influencing factors of the elderly's adoption intention of information technology for COVID-19 pandemic prevention and control, designed and collected 210 valid questionnaires, used SPSS 21.0 software to finish the descriptive statistical analysis, and used SmartPLS 3.0 software to finish the reliability and validity analysis of the questionnaires, and constructed a Structural Equation Model to verify the model. [Results/Conclusions] The study found that the risk perception, performance expectancy, effort expectancy, social influence and facilitating conditions had a significant impact on the elderly's behavioral intention of information technology for COVID-19 pandemic prevention and control , and technology anxiety, resistance to change had no significant impact on the behavioral intention. Finally, this paper puts forward countermeasures and suggestions based on the research findings.
Keywords	covid-19\|health informatics\|utaut\|structural equation model ; Bibliography. Library science. Information resources ; Z ; Agriculture ; S
Subject code	690 ; 303
Language	Chinese
Publishing date	2022-04-01T00:00:00Z
Publisher	Editorial Department of Journal of Library and Information Science in Agriculture
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Does the Tax Cuts Policy Lower Firm Survival Risk? Evidence from China

Wang Hong / Pang Jingni / Chen Jinghao / Huang Yangzhi

E3S Web of Conferences, Vol 409, p

2023 Volume 06012

Abstract: This paper examines the effect of tax cuts policy on firm survival risk. We analyze a panel data of China’s listed companies over the period 20082021 based on two different techniques: Kaplan-Meier survival estimator and the Cox proportional hazards ... ...

Abstract	This paper examines the effect of tax cuts policy on firm survival risk. We analyze a panel data of China’s listed companies over the period 20082021 based on two different techniques: Kaplan-Meier survival estimator and the Cox proportional hazards model. Our findings reveal that the tax cuts policy has positive and significant effect on improving firms’ survival probabilities. Moreover, compared with the income tax cuts, the value-added tax cuts play a more effective role on improving firms’ survival probability. By dividing firm locations into different regions, we also find that the tax cuts policy exerts a positive influence on firms’ survival probabilities in eastern region, while the positive effect in the central and western regions is relatively weaker. From the perspective of profitability heterogeneity, the results show that the beneficial effect from tax cuts policy on firm survival is more pronounced for firms with weaker profitability.
Keywords	taxation ; failure rate ; cox proportional hazards model ; Environmental sciences ; GE1-350
Subject code	336
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	EDP Sciences
Document type	Article ; Online
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Article ; Online: Exogenous brassinolide treatment regulates phenolic accumulation in mung bean sprouts through the modulation of sugar and energy metabolism

Wang, Hanbo / Chen, Jinghao / Guo, Runjiu / Wang, Dan / Wang, Taixia / Sun, Yali

Journal of the Science of Food and Agriculture. 2024 Feb., v. 104, no. 3 p.1656-1667

2024

Abstract: BACKGROUND: The effects of exogenous brassinolide (BR) treatment (3.0 μmol L⁻¹) on phenolic biosynthesis in mung bean sprouts were investigated. This investigation included the analysis of sugar content, substrates within the phenylpropane pathway, ... ...

Abstract	BACKGROUND: The effects of exogenous brassinolide (BR) treatment (3.0 μmol L⁻¹) on phenolic biosynthesis in mung bean sprouts were investigated. This investigation included the analysis of sugar content, substrates within the phenylpropane pathway, energy substances, enzymatic activity within the phenylpropane pathway, sugar metabolism and energy metabolism. RESULTS: Results showed that BR treatment significantly increased the levels of total phenolics, p‐hydroxybenzoic acid, p‐coumaric acid, gallic acid, fumalic acid and caffeic acid. This enhancement was accomplished through the elevation of l‐phenylalanine levels and the activation of enzymes associated with the phenylpropane pathway in mung bean sprouts, including phenylalanine ammonia‐lyase, cinnamate 4‐hydroxylase and 4‐coumarate CoA ligase. Furthermore, BR treatment induced alterations in sugar metabolism in mung bean sprouts as evidenced by the increased levels of glucose, fructose, sucrose and phosphoenolpyruvate. Moreover, increased activity was observed for enzymes linked to sucrose metabolism and glycolysis in the BR‐treated group. Concurrently, BR treatment bolstered the levels of adenosine triphosphate and energy charge in mung bean sprouts, which was attributed to the activation of H⁺‐adenosine triphosphatase, Ca²⁺‐adenosine triphosphatase and succinic dehydrogenase. CONCLUSION: These results suggest that BR treatment can accelerate the accumulation of phenolic compounds in mung bean sprouts. This effect is achieved not only through the activation of the phenylpropane pathway, but also through the modulation of sugar and energy metabolism. The modulation provides ample energy and a substrate for the biosynthesis of phenolics. © 2023 Society of Chemical Industry.
Keywords	4-hydroxybenzoic acid ; adenosine triphosphate ; agriculture ; biosynthesis ; brassinolide ; caffeic acid ; energy ; energy metabolism ; enzyme activity ; fructose ; gallic acid ; glucose ; glycolysis ; ligases ; mung beans ; p-coumaric acid ; phenylalanine ; phenylalanine ammonia-lyase ; sucrose ; sugar content ; trans-cinnamate 4-monooxygenase
Language	English
Dates of publication	2024-02
Size	p. 1656-1667.
Publishing place	John Wiley & Sons, Ltd.
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	184116-6
ISSN	1097-0010 ; 0022-5142
ISSN (online)	1097-0010
ISSN	0022-5142
DOI	10.1002/jsfa.13060
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Article ; Online: Applications of Genetic Code Expansion in Studying Protein Post-translational Modification.

Chen, Jinghao / Tsai, Yu-Hsuan

Journal of molecular biology

2021 Volume 434, Issue 8, Page(s) 167424

Abstract: Various post-translational modifications can naturally occur on proteins, regulating the activity, subcellular localization, interaction, or stability of the proteins. However, it can be challenging to decipher the biological implication or physiological ...

Abstract	Various post-translational modifications can naturally occur on proteins, regulating the activity, subcellular localization, interaction, or stability of the proteins. However, it can be challenging to decipher the biological implication or physiological roles of site-specific modifications due to their dynamic and sub-stoichiometric nature. Genetic code expansion method, relying on an orthogonal aminoacyl-tRNA synthetase/tRNA pair, enables site-specific incorporation of non-canonical amino acids. Here we focus on the application of genetic code expansion to study site-specific protein post-translational modification in vitro and in vivo. After a brief introduction, we discuss possibilities of incorporating non-canonical amino acids containing post-translational modifications or their mimics into target proteins. This approach is applicable for Ser/Thr/Tyr phosphorylation, Tyr sulfation/nitration/hydroxylation, Lys acetylation/acylation, Lys/His mono-methylation, as well as Arg citrullination. The next section describes the use of a precursor non-canonical amino acid followed by chemical and/or enzymatic reactions to afford the desired modification, such as Cys/Lys acylation, ubiquitin and ubiquitin-like modifications, as well as Lys/Gln methylation. We also discuss means for functional regulation of enzymes involving in post-translational modifications through genetically incorporated non-canonical amino acids. Lastly, the limitations and perspectives of genetic code expansion in studying protein post-translational modification are described.
MeSH term(s)	Amino Acids/genetics ; Amino Acyl-tRNA Synthetases/chemistry ; Genetic Code ; Protein Processing, Post-Translational/genetics ; Ubiquitin/metabolism
Chemical Substances	Amino Acids ; Ubiquitin ; Amino Acyl-tRNA Synthetases (EC 6.1.1.-)
Language	English
Publishing date	2021-12-28
Publishing country	Netherlands
Document type	Journal Article ; Review
ZDB-ID	80229-3
ISSN	1089-8638 ; 0022-2836
ISSN (online)	1089-8638
ISSN	0022-2836
DOI	10.1016/j.jmb.2021.167424
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Article: Applications of Genetic Code Expansion in Studying Protein Post-translational Modification

Chen, Jinghao / Tsai, Yu-Hsuan

Journal of molecular biology. 2021 Dec. 21,

2021

Abstract	Various post-translational modifications can naturally occur on proteins, regulating the activity, subcellular localization, interaction, or stability of the proteins. However, it can be challenging to decipher the biological implication or physiological roles of site-specific modifications due to their dynamic and sub-stoichiometric nature. Genetic code expansion method, relying on an orthogonal aminoacyl-tRNA synthetase/tRNA pair, enables site-specific incorporation of non-canonical amino acids. Here we focus on the application of genetic code expansion to study site-specific protein post-translational modification in vitro and in vivo. After a brief introduction, we discuss possibilities of incorporating non-canonical amino acids containing post-translational modifications or their mimics into target proteins. This approach is applicable for Ser/Thr/Tyr phosphorylation, Tyr sulfation/nitration/hydroxylation, Lys acetylation/acylation, Lys/His mono-methylation, as well as Arg citrullination. The next section describes the use of a precursor non-canonical amino acid followed by chemical and/or enzymatic reactions to afford the desired modification, such as Cys/Lys acylation, ubiquitin and ubiquitin-like modifications, as well as Lys/Gln methylation. We also discuss means for functional regulation of enzymes involving in post-translational modifications through genetically incorporated non-canonical amino acids. Lastly, the limitations and perspectives of genetic code expansion in studying protein post-translational modification are described.
Keywords	acetylation ; amino acids ; aminoacyl tRNA ligases ; genetic code ; hydroxylation ; methylation ; molecular biology ; phosphorylation ; post-translational modification ; ubiquitin
Language	English
Dates of publication	2021-1221
Publishing place	Elsevier Ltd
Document type	Article
Note	Pre-press version
ZDB-ID	80229-3
ISSN	1089-8638 ; 0022-2836
ISSN (online)	1089-8638
ISSN	0022-2836
DOI	10.1016/j.jmb.2021.167424
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Article ; Online: PIEZO1 regulates leader cell formation and cellular coordination during collective keratinocyte migration.

Chen, Jinghao / Holt, Jesse R / Evans, Elizabeth L / Lowengrub, John S / Pathak, Medha M

PLoS computational biology

2024 Volume 20, Issue 4, Page(s) e1011855

Abstract: The collective migration of keratinocytes during wound healing requires both the generation and transmission of mechanical forces for individual cellular locomotion and the coordination of movement across cells. Leader cells along the wound edge transmit ...

Abstract	The collective migration of keratinocytes during wound healing requires both the generation and transmission of mechanical forces for individual cellular locomotion and the coordination of movement across cells. Leader cells along the wound edge transmit mechanical and biochemical cues to ensuing follower cells, ensuring their coordinated direction of migration across multiple cells. Despite the observed importance of mechanical cues in leader cell formation and in controlling coordinated directionality of cell migration, the underlying biophysical mechanisms remain elusive. The mechanically-activated ion channel PIEZO1 was recently identified to play an inhibitory role during the reepithelialization of wounds. Here, through an integrative experimental and mathematical modeling approach, we elucidate PIEZO1's contributions to collective migration. Time-lapse microscopy reveals that PIEZO1 activity inhibits leader cell formation at the wound edge. To probe the relationship between PIEZO1 activity, leader cell formation and inhibition of reepithelialization, we developed an integrative 2D continuum model of wound closure that links observations at the single cell and collective cell migration scales. Through numerical simulations and subsequent experimental validation, we found that coordinated directionality plays a key role during wound closure and is inhibited by upregulated PIEZO1 activity. We propose that PIEZO1-mediated retraction suppresses leader cell formation which inhibits coordinated directionality between cells during collective migration.
MeSH term(s)	Cell Movement/physiology ; Keratinocytes ; Ion Channels
Chemical Substances	Ion Channels
Language	English
Publishing date	2024-04-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	2193340-6
ISSN	1553-7358 ; 1553-734X
ISSN (online)	1553-7358
ISSN	1553-734X
DOI	10.1371/journal.pcbi.1011855
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Article ; Online: Selective Inhibition of Kinase Activity in Mammalian Cells by Bioorthogonal Ligand Tethering.

Chen, Jinghao / Huang, Yang / Gan, Wen-Biao / Tsai, Yu-Hsuan

Methods in molecular biology (Clifton, N.J.)

2023 Volume 2676, Page(s) 215–232

Abstract: Enzymes are critical for cellular functions, and malfunction of enzymes is closely related to many human diseases. Inhibition studies can help in deciphering the physiological roles of enzymes and guide conventional drug development programs. In ... ...

Abstract	Enzymes are critical for cellular functions, and malfunction of enzymes is closely related to many human diseases. Inhibition studies can help in deciphering the physiological roles of enzymes and guide conventional drug development programs. In particular, chemogenetic approaches enabling rapid and selective inhibition of enzymes in mammalian cells have unique advantages. Here, we describe the procedure for rapid and selective inhibition of a kinase in mammalian cells by bioorthogonal ligand tethering (iBOLT). Briefly, a non-canonical amino acid bearing a bioorthogonal group is genetically incorporated into the target kinase by genetic code expansion. The sensitized kinase can react with a conjugate containing a complementary biorthogonal group linked with a known inhibitory ligand. As a result, tethering of the conjugate to the target kinase allows selective inhibition of protein function. Here, we demonstrate this method by using cAMP-dependent protein kinase catalytic subunit alpha (PKA-Cα) as the model enzyme. The method should be applicable to other kinases, enabling their rapid and selective inhibition.
MeSH term(s)	Animals ; Humans ; Ligands ; Proteins/chemistry ; Phosphorylation ; Amino Acids/chemistry ; Mammals/metabolism
Chemical Substances	Ligands ; Proteins ; Amino Acids
Language	English
Publishing date	2023-06-05
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-3251-2_16
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Nerve-on-a-Chip Derived Biomimicking Microfibers for Peripheral Nerve Regeneration.

Yu, Yunru / Jin, Binghui / Chen, Jinghao / Lou, Chenghao / Guo, Jiahui / Yang, Chaoyu / Zhao, Yuanjin

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

2023 Volume 10, Issue 20, Page(s) e2207536

Abstract: Fibrous scaffolds have shown their advantages in tissue engineering, such as peripheral nerve regeneration, while most of the existing fiber-shaped scaffolds are with simple structures, and the in vitro performance for nerve regeneration lacks systematic ...

Abstract	Fibrous scaffolds have shown their advantages in tissue engineering, such as peripheral nerve regeneration, while most of the existing fiber-shaped scaffolds are with simple structures, and the in vitro performance for nerve regeneration lacks systematic analysis. Here, novel nerve-on-a-chip derived biomimicking microfibers for peripheral nerve regeneration are presented. The microfibers with controllable core-shell structures and functionalities are generated through capillary microfluidic devices. By integrating these microfibers into a multitrack-architectured chip, and coculturing them with nerve cells as well as gradient bioactive elements, the nerve-on-a-chip with the capabilities of systematically assessing the performances of nerve fiber formation in the hollow microfibers at in vitro level is constructed. Based on a rat sciatic nerve injury model, the rapid promotion ability is demonstrated of optimized microfibers in nerve regeneration and function recovery in vivo, which implies the credibility of the nerve-on-a-chip on biomimicking microfibers evaluation for peripheral nerve regeneration. Thus, it is convinced that the organ-on-a-chip will undoubtedly open up a new chapter in evaluating biological scaffolds for in vivo tissue engineering.
MeSH term(s)	Rats ; Animals ; Tissue Engineering ; Nerve Regeneration ; Lab-On-A-Chip Devices
Language	English
Publishing date	2023-04-29
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2808093-2
ISSN	2198-3844 ; 2198-3844
ISSN (online)	2198-3844
ISSN	2198-3844
DOI	10.1002/advs.202207536
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Article ; Online: Exploring the Functioning of Online Self-Organizations during Public Health Emergencies: Patterns and Mechanism.

Chen, Jinghao / Liu, Qianxi / Liu, Xiaoyan / Wang, Youfeng / Nie, Huizi / Xie, Xiankun

International journal of environmental research and public health

2023 Volume 20, Issue 5

Abstract: With the increasing use of social media, online self-organized relief has become a crucial aspect of crisis management during public health emergencies, leading to the emergence of online self-organizations. This study employed the BERT model to classify ...

Abstract	With the increasing use of social media, online self-organized relief has become a crucial aspect of crisis management during public health emergencies, leading to the emergence of online self-organizations. This study employed the BERT model to classify the replies of Weibo users and used K-means clustering to summarize the patterns of self-organized groups and communities. We then combined the findings from pattern discovery and documents from online relief networks to analyze the core components and mechanisms of online self-organizations. Our findings indicate the following: (1) The composition of online self-organized groups follows Pareto's law. (2) Online self-organized communities are mainly composed of sparse and small groups with loose connections, and bot accounts can automatically identify those in need and provide them with helpful information and resources. (3) The core components of the mechanism of online self-organized rescue groups include the initial gathering of groups, the formation of key groups, the generation of collective action, and the establishment of organizational norms. This study suggests that social media can establish an authentication mechanism for online self-organizations, and that authorities should encourage online interactive live streams about public health issues. However, it is important to note that self-organizations are not a panacea for all issues during public health emergencies.
MeSH term(s)	Humans ; Public Health/methods ; Emergencies ; Personality ; Social Media
Language	English
Publishing date	2023-02-23
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2175195-X
ISSN	1660-4601 ; 1661-7827
ISSN (online)	1660-4601
ISSN	1661-7827
DOI	10.3390/ijerph20054012
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Article ; Online: Exogenous brassinolide treatment regulates phenolic accumulation in mung bean sprouts through the modulation of sugar and energy metabolism.

Wang, Hanbo / Chen, Jinghao / Guo, Runjiu / Wang, Dan / Wang, Taixia / Sun, Yali

Journal of the science of food and agriculture

2023 Volume 104, Issue 3, Page(s) 1656–1667

Abstract: Background: The effects of exogenous brassinolide (BR) treatment (3.0 μmol L: Results: Results showed that BR treatment significantly increased the levels of total phenolics, p-hydroxybenzoic acid, p-coumaric acid, gallic acid, fumalic acid and ... ...

Abstract	Background: The effects of exogenous brassinolide (BR) treatment (3.0 μmol L Results: Results showed that BR treatment significantly increased the levels of total phenolics, p-hydroxybenzoic acid, p-coumaric acid, gallic acid, fumalic acid and caffeic acid. This enhancement was accomplished through the elevation of l-phenylalanine levels and the activation of enzymes associated with the phenylpropane pathway in mung bean sprouts, including phenylalanine ammonia-lyase, cinnamate 4-hydroxylase and 4-coumarate CoA ligase. Furthermore, BR treatment induced alterations in sugar metabolism in mung bean sprouts as evidenced by the increased levels of glucose, fructose, sucrose and phosphoenolpyruvate. Moreover, increased activity was observed for enzymes linked to sucrose metabolism and glycolysis in the BR-treated group. Concurrently, BR treatment bolstered the levels of adenosine triphosphate and energy charge in mung bean sprouts, which was attributed to the activation of H Conclusion: These results suggest that BR treatment can accelerate the accumulation of phenolic compounds in mung bean sprouts. This effect is achieved not only through the activation of the phenylpropane pathway, but also through the modulation of sugar and energy metabolism. The modulation provides ample energy and a substrate for the biosynthesis of phenolics. © 2023 Society of Chemical Industry.
MeSH term(s)	Vigna/chemistry ; Sugars/metabolism ; Energy Metabolism ; Sucrose/metabolism ; Adenosine Triphosphatases/metabolism
Chemical Substances	brassinolide (Y9IQ1L53OX) ; Sugars ; Sucrose (57-50-1) ; Adenosine Triphosphatases (EC 3.6.1.-)
Language	English
Publishing date	2023-11-06
Publishing country	England
Document type	Journal Article
ZDB-ID	184116-6
ISSN	1097-0010 ; 0022-5142
ISSN (online)	1097-0010
ISSN	0022-5142
DOI	10.1002/jsfa.13060
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