LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 6 of total 6

Search options

  1. Article ; Online: Gastro-Intestinal Digested Bovine Milk Osteopontin Modulates Gut Barrier Biomarkers In Vitro.

    Li, Lu / Chen, Juchun / Zheng, Yuxing / Lane, Jonathan / Hu, Ruibiao / Zhu, Jianzhong / Fu, Xiong / Huang, Qiang / Liu, Feitong / Zhang, Bin

    Molecular nutrition & food research

    2024  Volume 68, Issue 4, Page(s) e2200777

    Abstract: Scope: Osteopontin (OPN) is a multifunctional protein naturally present in mammals' milk, associated with immune homeostasis and intestinal maturation. This study aims to investigate the protein digestion pattern and the cellular bioactivity of bovine ... ...

    Abstract Scope: Osteopontin (OPN) is a multifunctional protein naturally present in mammals' milk, associated with immune homeostasis and intestinal maturation. This study aims to investigate the protein digestion pattern and the cellular bioactivity of bovine milk OPN digesta in vitro.
    Methods and results: A modified INFOGEST static in vitro infant digestion protocol and a Caco-2/HT-29 co-culture cell model are employed to evaluate the digestion properties and the anti-inflammatory effects of OPN. OPN is resistant to gastric hydrolysis but degraded into large peptides during intestinal digestion. Its 10 kDa digesta permeate with predicted extensive bioactivities protects the co-culture cell model from the inflammation-induced dysfunction by dose-dependently recovering the expression of occludin, claudin-3, and ZO-1. Low dosage of OPN significantly decreases the production of IL-8 and IL-6, and downregulates the mRNA and protein expression of MyD88, NF-κB p65, and IκB-α, whereas a high dose evokes a mild pro-inflammatory response. Interestingly, anti-inflammatory effect of OPN digesta is stronger than lactoferrin and whey protein concentrate counterparts.
    Conclusion: The findings demonstrate that the bioactive peptides released from in vitro infant gastrointestinal digestion of bovine milk OPN alleviates intestinal epithelial cell inflammation by inhibiting NF-κB pathway activation and potentiates the barrier function of the intestinal epithelium.
    MeSH term(s) Humans ; Infant ; Animals ; Milk/chemistry ; Caco-2 Cells ; NF-kappa B ; Osteopontin/genetics ; Osteopontin/metabolism ; Inflammation ; Biomarkers/analysis ; Anti-Inflammatory Agents ; Mammals/metabolism
    Chemical Substances NF-kappa B ; Osteopontin (106441-73-0) ; Biomarkers ; Anti-Inflammatory Agents
    Language English
    Publishing date 2024-01-09
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2160372-8
    ISSN 1613-4133 ; 1613-4125
    ISSN (online) 1613-4133
    ISSN 1613-4125
    DOI 10.1002/mnfr.202200777
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article: Longitudinal changes of human milk oligosaccharides, breastmilk microbiome and infant gut microbiome are associated with maternal characteristics

    Liu, Feitong / He, Shiting / Yan, Jingyu / Yan, Shuyuan / Chen, Juchun / Lu, Zerong / Zhang, Bin / Lane, Jonathan

    International journal of food science & technology. 2022 May, v. 57, no. 5

    2022  

    Abstract: Human milk oligosaccharides (HMOs) play an important role in infant health. This study aimed to investigate the association of maternal characteristics with HMOs, human breastmilk (HBM) microbiome and infant gut microbiome over the first three months of ... ...

    Abstract Human milk oligosaccharides (HMOs) play an important role in infant health. This study aimed to investigate the association of maternal characteristics with HMOs, human breastmilk (HBM) microbiome and infant gut microbiome over the first three months of lactation. Chinese mothers and infant pairs (n = 110) were included in this prospective cohort. Secretor status linked with α1,2‐fucosyltransferase expression was determined by the presence of total α1,2‐fucosylated HMOs in HBM for 75.8% of the mothers. The concentration of dominant HMOs significantly decreased over three months except for 3’‐fucosyllactose. In addition to the elevated levels of α1,2‐fucosylated HMOs, other neutral HMOs significantly reduced in secretors milk. Alpha‐diversity of HBM and infant gut microbiome significantly increased over time, and an elevated abundance of Bifidobacterium and decreased levels of Streptococcus, Staphylococcus and Clostridium in the infant gut microbiota were noted. Multi‐association analysis indicated maternal age and body mass index significantly correlated with specific HMOs and infant growth. Our study provides pivotal data on Chinese HMOs distribution profile, and their association with maternal characteristics and the infant gut microbiome.
    Keywords Bifidobacterium ; Clostridium ; Staphylococcus ; Streptococcus ; body mass index ; breast milk ; food science ; humans ; infant growth ; intestinal microorganisms ; lactation ; microbiome ; milk ; oligosaccharides ; species diversity ; technology
    Language English
    Dates of publication 2022-05
    Size p. 2793-2807.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 883561-5
    ISSN 0950-5423
    ISSN 0950-5423
    DOI 10.1111/ijfs.15324
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 2121: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article: Xiang Study: an association of breastmilk composition with maternal body mass index and infant growth during the first 3 month of life.

    Peng, Xuyi / Li, Jie / Yan, Shuyuan / Chen, Juchun / Lane, Jonathan / Malard, Patrice / Liu, Feitong

    Nutrition research and practice

    2020  Volume 15, Issue 3, Page(s) 367–381

    Abstract: Background/objectives: This study aimed to establish a mother and child cohort in the Chinese population, and investigate human breastmilk (HBM) composition and its relationship with maternal body mass index (BMI) and infant growth during the first 3 ... ...

    Abstract Background/objectives: This study aimed to establish a mother and child cohort in the Chinese population, and investigate human breastmilk (HBM) composition and its relationship with maternal body mass index (BMI) and infant growth during the first 3 mon of life.
    Subjects/methods: A total of 101 Chinese mother and infant pairs were included in this prospective cohort. Alterations in the milk macronutrients of Chinese mothers at 1 mon (T1), 2 mon (T2), and 3 mon (T3) lactation were analyzed. HBM fatty acid (FA) profiles were measured by gas chromatography (GC), and HBM proteomic profiling was achieved by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS).
    Results: During the first 3 mon of lactation (
    Conclusion: This study provides additional evidence to the Chinese HBM database, and further knowledge of FA function. It also helps to establish future maternal strategies that support the healthy growth and development of Chinese infants.
    Language English
    Publishing date 2020-10-06
    Publishing country Korea (South)
    Document type Journal Article
    ZDB-ID 2387946-4
    ISSN 2005-6168 ; 1976-1457
    ISSN (online) 2005-6168
    ISSN 1976-1457
    DOI 10.4162/nrp.2021.15.3.367
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 7762: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  4. Article: In vitro fermentation of human milk oligosaccharides by individual Bifidobacterium longum-dominant infant fecal inocula

    Li, Hongye / Lane, Jonathan A. / Chen, Juchun / Lu, Zerong / Wang, Hongwei / Dhital, Sushil / Fu, Xiong / Huang, Qiang / Liu, Feitong / Zhang, Bin

    Carbohydrate polymers. 2022 July 01, v. 287

    2022  

    Abstract: This study investigated the fermentation characteristics and microbial responses of human milk oligosaccharides (HMOs) by individual Bifidobacterium longum-dominant infant fecal microbiota. Fucosylated neutral HMOs (2′-fucosyllactose, 2′-FL; 3- ... ...

    Abstract This study investigated the fermentation characteristics and microbial responses of human milk oligosaccharides (HMOs) by individual Bifidobacterium longum-dominant infant fecal microbiota. Fucosylated neutral HMOs (2′-fucosyllactose, 2′-FL; 3-fucosyllactose, 3-FL), sialylated HMOs (3′-sialyllactose, 3′-SL; 6′-sialyllactose, 6′-SL), and non-fucosylated neutral HMOs (Lacto-N-tetraose, LNT; Lacto-N-neotetraose, LNnT) were fermented in vitro, with fructooligosaccharides (FOS) and galactooligosaccharides (GOS) as positive controls. The fermentation rate was not affected by the molecular specificity of HMOs. Acetate (98–104 mM) and lactate (9–19 mM) were the primary metabolites at the end of fermentation. All six HMOs showed the same levels of acetate production, but sialylated HMOs produced significantly less lactate than neutral HMOs. HMOs and GOS could maintain the dominance or increase the relative abundance of Bifidobacterium longum, while FOS remarkably promote Klebsiella pneumoniae with the highest gas production and the least acetate and lactate yield. The findings are supportive to optimize infant nutrition strategies for enhanced functions.
    Keywords Bifidobacterium longum ; Klebsiella pneumoniae ; acetates ; breast milk ; fermentation ; fructooligosaccharides ; galactooligosaccharides ; infant nutrition ; inoculum ; intestinal microorganisms ; lactic acid ; metabolites
    Language English
    Dates of publication 2022-0701
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1501516-6
    ISSN 1879-1344 ; 0144-8617
    ISSN (online) 1879-1344
    ISSN 0144-8617
    DOI 10.1016/j.carbpol.2022.119322
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article ; Online: In Vitro Infant Fecal Fermentation Characteristics of Human Milk Oligosaccharides Were Controlled by Initial Microbiota Composition More than Chemical Structure.

    Xu, Shiqi / Lane, Jonathan A / Chen, Juchun / Zheng, Yuxing / Wang, Hongwei / Fu, Xiong / Huang, Qiang / Dhital, Sushil / Liu, Feitong / Zhang, Bin

    Molecular nutrition & food research

    2022  Volume 66, Issue 19, Page(s) e2200098

    Abstract: Scope: Human milk oligosaccharides (HMOs), multifunctional glycans naturally present in human milk, are known to contribute to the infant's microbiota and immune system development. However, the molecular specificity of HMOs on microbiota and associated ...

    Abstract Scope: Human milk oligosaccharides (HMOs), multifunctional glycans naturally present in human milk, are known to contribute to the infant's microbiota and immune system development. However, the molecular specificity of HMOs on microbiota and associated fermentation is not yet fully understood, and is important for the development of infant formula optimum functionality.
    Methods and results: In vitro fermentation is carried out on structurally different HMOs with infant fecal inocula dominated by Bifidobacterium longum, Bifidobacterium breve, and Bacteroides. The gas, metabolite (SCFA, lactate, and succinate) profiles, and microbiota responses differ between individual microbiota inocula patterns regardless of HMO structure. In terms of HMO pairs with same sugar composition but different glycosidic bonds, gas and metabolite profiles are similar with the B. longum- and B. breve-dominated inocula. However, large individual variations are observed with the Bacteroides-dominated inocula. The microbial communities at the end of fermentation are closely related to the initial microbiota composition.
    Conclusion: The findings demonstrate that short-term in vitro fermentation outcomes largely depend on the initial gut microbiota composition more than the impact of HMO molecular specificity. These results advance the current understanding for the design of personalized infant nutritional solutions and therapies in future.
    MeSH term(s) Bacteroides ; Fermentation ; Humans ; Infant ; Lactates ; Microbiota ; Milk, Human/chemistry ; Oligosaccharides/metabolism ; Succinates ; Sugars
    Chemical Substances Lactates ; Oligosaccharides ; Succinates ; Sugars
    Language English
    Publishing date 2022-08-31
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2160372-8
    ISSN 1613-4133 ; 1613-4125
    ISSN (online) 1613-4133
    ISSN 1613-4125
    DOI 10.1002/mnfr.202200098
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article ; Online: In vitro fermentation of human milk oligosaccharides by individual Bifidobacterium longum-dominant infant fecal inocula.

    Li, Hongye / Lane, Jonathan A / Chen, Juchun / Lu, Zerong / Wang, Hongwei / Dhital, Sushil / Fu, Xiong / Huang, Qiang / Liu, Feitong / Zhang, Bin

    Carbohydrate polymers

    2022  Volume 287, Page(s) 119322

    Abstract: This study investigated the fermentation characteristics and microbial responses of human milk oligosaccharides (HMOs) by individual Bifidobacterium longum-dominant infant fecal microbiota. Fucosylated neutral HMOs (2'-fucosyllactose, 2'-FL; 3- ... ...

    Abstract This study investigated the fermentation characteristics and microbial responses of human milk oligosaccharides (HMOs) by individual Bifidobacterium longum-dominant infant fecal microbiota. Fucosylated neutral HMOs (2'-fucosyllactose, 2'-FL; 3-fucosyllactose, 3-FL), sialylated HMOs (3'-sialyllactose, 3'-SL; 6'-sialyllactose, 6'-SL), and non-fucosylated neutral HMOs (Lacto-N-tetraose, LNT; Lacto-N-neotetraose, LNnT) were fermented in vitro, with fructooligosaccharides (FOS) and galactooligosaccharides (GOS) as positive controls. The fermentation rate was not affected by the molecular specificity of HMOs. Acetate (98-104 mM) and lactate (9-19 mM) were the primary metabolites at the end of fermentation. All six HMOs showed the same levels of acetate production, but sialylated HMOs produced significantly less lactate than neutral HMOs. HMOs and GOS could maintain the dominance or increase the relative abundance of Bifidobacterium longum, while FOS remarkably promote Klebsiella pneumoniae with the highest gas production and the least acetate and lactate yield. The findings are supportive to optimize infant nutrition strategies for enhanced functions.
    MeSH term(s) Bifidobacterium longum/metabolism ; Fermentation ; Humans ; Infant ; Lactic Acid ; Milk, Human/metabolism ; Oligosaccharides/metabolism
    Chemical Substances Oligosaccharides ; Lactic Acid (33X04XA5AT)
    Language English
    Publishing date 2022-03-08
    Publishing country England
    Document type Journal Article
    ZDB-ID 1501516-6
    ISSN 1879-1344 ; 0144-8617
    ISSN (online) 1879-1344
    ISSN 0144-8617
    DOI 10.1016/j.carbpol.2022.119322
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top