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  1. Article ; Online: An autocatalytic CRISPR-Cas amplification effect propelled by the LNA-modified split activators for DNA sensing.

    Sun, Ke / Pu, Lei / Chen, Chuan / Chen, Mutian / Li, Kaiju / Li, Xinqiong / Li, Huanqing / Geng, Jia

    Nucleic acids research

    2024  Volume 52, Issue 7, Page(s) e39

    Abstract: CRISPR-Cas systems with dual functions offer precise sequence-based recognition and efficient catalytic cleavage of nucleic acids, making them highly promising in biosensing and diagnostic technologies. However, current methods encounter challenges of ... ...

    Abstract CRISPR-Cas systems with dual functions offer precise sequence-based recognition and efficient catalytic cleavage of nucleic acids, making them highly promising in biosensing and diagnostic technologies. However, current methods encounter challenges of complexity, low turnover efficiency, and the necessity for sophisticated probe design. To better integrate the dual functions of Cas proteins, we proposed a novel approach called CRISPR-Cas Autocatalysis Amplification driven by LNA-modified Split Activators (CALSA) for the highly efficient detection of single-stranded DNA (ssDNA) and genomic DNA. By introducing split ssDNA activators and the site-directed trans-cleavage mediated by LNA modifications, an autocatalysis-driven positive feedback loop of nucleic acids based on the LbCas12a system was constructed. Consequently, CALSA enabled one-pot and real-time detection of genomic DNA and cell-free DNA (cfDNA) from different tumor cell lines. Notably, CALSA achieved high sensitivity, single-base specificity, and remarkably short reaction times. Due to the high programmability of nucleic acid circuits, these results highlighted the immense potential of CALSA as a powerful tool for cascade signal amplification. Moreover, the sensitivity and specificity further emphasized the value of CALSA in biosensing and diagnostics, opening avenues for future clinical applications.
    MeSH term(s) CRISPR-Cas Systems ; Humans ; Oligonucleotides/chemistry ; Oligonucleotides/genetics ; DNA, Single-Stranded/genetics ; DNA, Single-Stranded/metabolism ; DNA, Single-Stranded/chemistry ; Biosensing Techniques/methods ; Nucleic Acid Amplification Techniques/methods ; DNA/chemistry ; DNA/genetics ; Cell Line, Tumor ; Catalysis
    Chemical Substances locked nucleic acid ; Oligonucleotides ; DNA, Single-Stranded ; DNA (9007-49-2)
    Language English
    Publishing date 2024-04-16
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 186809-3
    ISSN 1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048
    ISSN (online) 1362-4962 ; 1362-4954
    ISSN 0301-5610 ; 0305-1048
    DOI 10.1093/nar/gkae176
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1067: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: E. coli outer membrane protein T (OmpT) nanopore for peptide sensing.

    Chen, Chuan / Song, Mengxiao / Li, Kaiju / Yan, Shixin / Chen, Mutian / Geng, Jia

    Biochemical and biophysical research communications

    2023  Volume 677, Page(s) 132–140

    Abstract: Peptide detection methods with facility and high sensitivity are essential for diagnosing disease associated with peptide biomarkers. Nanopore sensing technology had emerged as a low cost, high-throughput, and scalable tool for peptide detection. The ... ...

    Abstract Peptide detection methods with facility and high sensitivity are essential for diagnosing disease associated with peptide biomarkers. Nanopore sensing technology had emerged as a low cost, high-throughput, and scalable tool for peptide detection. The omptins family proteins which can form β-barrel pores have great potentials to be developed as nanopore biosensor. However, there are no study about the channel properties of E. coli OmpT and the development of OmpT as a nanopore biosensor. In this study, the OmpT biological nanopore channel was constructed with a conductance of 1.49 nS in 500 mM NaCl buffer and a three-step gating phenomenon under negative voltage higher than 100 mV and then was developed as a peptide biosensor which can detect peptide without the interfere of ssDNA and dNTPs. The OmpT constructed in this study has potential application in peptide detection, and also provides a new idea for the detection of peptides using the specific binding ability of protease.
    MeSH term(s) Escherichia coli/metabolism ; Escherichia coli Proteins/metabolism ; Nanopores ; Bacterial Outer Membrane Proteins/genetics ; Peptides/metabolism
    Chemical Substances Escherichia coli Proteins ; Bacterial Outer Membrane Proteins ; Peptides
    Language English
    Publishing date 2023-06-30
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 205723-2
    ISSN 1090-2104 ; 0006-291X ; 0006-291X
    ISSN (online) 1090-2104 ; 0006-291X
    ISSN 0006-291X
    DOI 10.1016/j.bbrc.2023.05.125
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 116: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Autophagy is regulated by endoplasmic reticulum calcium homeostasis and sphingolipid metabolism.

    Liu, Shiyan / Chen, Mutian / Wang, Yichang / Li, Huihui / Qi, Shiqian / Geng, Jia / Lu, Kefeng

    Autophagy

    2023  Volume 19, Issue 12, Page(s) 3256–3257

    Abstract: Calcium is involved in a variety of cellular processes. As the crucial components of cell membranes, sphingolipids also play important roles as signaling molecules. Intracellular calcium homeostasis, autophagy initiation and sphingolipid synthesis are ... ...

    Abstract Calcium is involved in a variety of cellular processes. As the crucial components of cell membranes, sphingolipids also play important roles as signaling molecules. Intracellular calcium homeostasis, autophagy initiation and sphingolipid synthesis are associated with the endoplasmic reticulum (ER). Recently, through genetic screening and lipidomics analysis in
    MeSH term(s) Calcium/metabolism ; Autophagy ; Sphingolipids ; Saccharomyces cerevisiae/metabolism ; Endoplasmic Reticulum/metabolism ; Calcium Channels/metabolism ; Homeostasis
    Chemical Substances Calcium (SY7Q814VUP) ; Sphingolipids ; Calcium Channels
    Language English
    Publishing date 2023-08-23
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2454135-7
    ISSN 1554-8635 ; 1554-8627
    ISSN (online) 1554-8635
    ISSN 1554-8627
    DOI 10.1080/15548627.2023.2249761
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6741: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Direct single-molecule detection of CoA-SH and ATP by the membrane proteins TMEM120A and TMEM120B.

    Zhao, Changjian / Chen, Mutian / Liu, Xiaofeng / Yuan, Weidan / Li, Kaiju / Wang, Yu / Chen, Chen / Zhang, Ming / Dong, Yuhan / Xiao, Yuling / Deng, Dong / Geng, Jia

    Nanoscale

    2024  Volume 16, Issue 12, Page(s) 6087–6094

    Abstract: Membrane proteins are vital resources for developing biosensors. TMEM120A is a membrane protein associated with human pain transmission and lipid metabolism, and recent studies have demonstrated its ability to transport ions and bind to coenzyme A (COA- ... ...

    Abstract Membrane proteins are vital resources for developing biosensors. TMEM120A is a membrane protein associated with human pain transmission and lipid metabolism, and recent studies have demonstrated its ability to transport ions and bind to coenzyme A (COA-SH), indicating its potential to develop into a single-molecule sensor based on electrical methods. In this study, we investigated the ion transport properties of TMEM120A and its homolog TMEM120B on an artificial lipid bilayer using single-channel recording. The results demonstrate that both proteins can fuse into the lipid bilayer and generate stable ion currents under a bias voltage. Based on the stable ion transport capabilities of TMEM120A and TMEM120B, as well as the feature of TMEM120A binding with COA-SH, we developed these two proteins into a single-molecule sensor for detecting COA-SH and structurally similar molecules. We found that both COA-SH and ATP can reversibly bind to single TMEM120A and TMEM120B proteins embedded in the lipid bilayer and temporarily block ion currents during the binding process. By analyzing the current blocking signal, COA-SH and ATP can be identified at the single-molecule level. In conclusion, our work has provided two single-molecule biosensors for detecting COA-SH and ATP, offering insights for exploring and developing bio-inspired small molecule sensors.
    MeSH term(s) Humans ; Lipid Bilayers/chemistry ; Membrane Proteins/metabolism ; Coenzyme A ; Nanotechnology ; Adenosine Triphosphate
    Chemical Substances Lipid Bilayers ; Membrane Proteins ; Coenzyme A (SAA04E81UX) ; Adenosine Triphosphate (8L70Q75FXE)
    Language English
    Publishing date 2024-03-21
    Publishing country England
    Document type Journal Article
    ZDB-ID 2515664-0
    ISSN 2040-3372 ; 2040-3364
    ISSN (online) 2040-3372
    ISSN 2040-3364
    DOI 10.1039/d3nr05054h
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Direct and Continuous Monitoring of Multicomponent Antibiotic Gentamicin in Blood at Single-Molecule Resolution.

    Zhao, Changjian / Wang, Yu / Chen, Chen / Zhu, Yibo / Miao, Zhuang / Mou, Xingyu / Yuan, Weidan / Zhang, Zhihao / Li, Kaiju / Chen, Mutian / Liang, Weibo / Zhang, Ming / Miao, Wenqian / Dong, Yuhan / Deng, Dong / Wu, Jianping / Ke, Bowen / Bao, Rui / Geng, Jia

    ACS nano

    2024  Volume 18, Issue 12, Page(s) 9137–9149

    Abstract: Point-of-care monitoring of small molecules in biofluids is crucial for clinical diagnosis and treatment. However, the inherent low degree of recognition of small molecules and the complex composition of biofluids present significant obstacles for ... ...

    Abstract Point-of-care monitoring of small molecules in biofluids is crucial for clinical diagnosis and treatment. However, the inherent low degree of recognition of small molecules and the complex composition of biofluids present significant obstacles for current detection technologies. Although nanopore sensing excels in the analysis of small molecules, the direct detection of small molecules in complex biofluids remains a challenge. In this study, we present a method for sensing the small molecule drug gentamicin in whole blood based on the mechanosensitive channel of small conductance in
    MeSH term(s) Humans ; Rats ; Animals ; Anti-Bacterial Agents/pharmacology ; Gentamicins ; Nanotechnology ; Nanopores ; Pseudomonas aeruginosa
    Chemical Substances Anti-Bacterial Agents ; Gentamicins
    Language English
    Publishing date 2024-03-12
    Publishing country United States
    Document type Journal Article
    ISSN 1936-086X
    ISSN (online) 1936-086X
    DOI 10.1021/acsnano.4c00302
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  6. Article ; Online: The ER calcium channel Csg2 integrates sphingolipid metabolism with autophagy.

    Liu, Shiyan / Chen, Mutian / Wang, Yichang / Lei, Yuqing / Huang, Ting / Zhang, Yabin / Lam, Sin Man / Li, Huihui / Qi, Shiqian / Geng, Jia / Lu, Kefeng

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 3725

    Abstract: Sphingolipids are ubiquitous components of membranes and function as bioactive lipid signaling molecules. Here, through genetic screening and lipidomics analyses, we find that the endoplasmic reticulum (ER) calcium channel Csg2 integrates sphingolipid ... ...

    Abstract Sphingolipids are ubiquitous components of membranes and function as bioactive lipid signaling molecules. Here, through genetic screening and lipidomics analyses, we find that the endoplasmic reticulum (ER) calcium channel Csg2 integrates sphingolipid metabolism with autophagy by regulating ER calcium homeostasis in the yeast Saccharomyces cerevisiae. Csg2 functions as a calcium release channel and maintains calcium homeostasis in the ER, which enables normal functioning of the essential sphingolipid synthase Aur1. Under starvation conditions, deletion of Csg2 causes increases in calcium levels in the ER and then disturbs Aur1 stability, leading to accumulation of the bioactive sphingolipid phytosphingosine, which specifically and completely blocks autophagy and induces loss of starvation resistance in cells. Our findings indicate that calcium homeostasis in the ER mediated by the channel Csg2 translates sphingolipid metabolism into autophagy regulation, further supporting the role of the ER as a signaling hub for calcium homeostasis, sphingolipid metabolism and autophagy.
    MeSH term(s) Autophagy ; Calcium/metabolism ; Endoplasmic Reticulum/metabolism ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Saccharomyces cerevisiae Proteins/genetics ; Saccharomyces cerevisiae Proteins/metabolism ; Sphingolipids/metabolism
    Chemical Substances Calcium (SY7Q814VUP) ; Saccharomyces cerevisiae Proteins ; Sphingolipids ; CSG2 protein, S cerevisiae
    Language English
    Publishing date 2023-06-22
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-39482-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Effect of water adsorption at nanoparticle–oil interface on charge transport in high humidity transformer oil-based nanofluid

    Du, Yuefan / Lv, Yuzhen / Li, Chengrong / Zhong, Yuxiang / Chen, Mutian / Zhang, Shengnan / Zhou, You / Chen, Zhengqi

    Colloids and surfaces. 2012 Dec. 5, v. 415

    2012  

    Abstract: Transformer oil-based nanofluids (NFs) with TiO₂ semiconductive nanoparticles (SNFs) exhibit substantially higher AC breakdown voltage than that of pure transformer oils at variable relative humidity from 20% to 80%. Charge accumulation and decay ... ...

    Abstract Transformer oil-based nanofluids (NFs) with TiO₂ semiconductive nanoparticles (SNFs) exhibit substantially higher AC breakdown voltage than that of pure transformer oils at variable relative humidity from 20% to 80%. Charge accumulation and decay characteristics of pure oils and SNFs were measured by the pulse electroacoustic technique (PEA). It reveals that SNFs have more uniform internal electric fields and higher charge decay rate compared to pure oils under high relative humidity. It is confirmed by the test results of electrophoresis and thermally stimulated current (TSC) that the nanoparticles adsorb water molecules at the nanoparticle–oil interface, giving rise to the higher shallow trap density and resulting in better charge transport in SNFs.
    Keywords adsorption ; colloids ; electric field ; electrophoresis ; nanoparticles ; oils ; relative humidity ; titanium dioxide
    Language English
    Dates of publication 2012-1205
    Size p. 153-158.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1500517-3
    ISSN 0927-7757
    ISSN 0927-7757
    DOI 10.1016/j.colsurfa.2012.10.002
    Database NAL-Catalogue (AGRICOLA)

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  8. Article: Effect of water adsorption at nanoparticle–oil interface on charge transport in high humidity transformer oil-based nanofluid

    Du, Yuefan / Lv, Yuzhen / Li, Chengrong / Zhong, Yuxiang / Chen, Mutian / Zhang, Shengnan / Zhou, You / Chen, Zhengqi

    Colloids and surfaces

    Volume v. 415

    Abstract: Transformer oil-based nanofluids (NFs) with TiO₂ semiconductive nanoparticles (SNFs) exhibit substantially higher AC breakdown voltage than that of pure transformer oils at variable relative humidity from 20% to 80%. Charge accumulation and decay ... ...

    Abstract Transformer oil-based nanofluids (NFs) with TiO₂ semiconductive nanoparticles (SNFs) exhibit substantially higher AC breakdown voltage than that of pure transformer oils at variable relative humidity from 20% to 80%. Charge accumulation and decay characteristics of pure oils and SNFs were measured by the pulse electroacoustic technique (PEA). It reveals that SNFs have more uniform internal electric fields and higher charge decay rate compared to pure oils under high relative humidity. It is confirmed by the test results of electrophoresis and thermally stimulated current (TSC) that the nanoparticles adsorb water molecules at the nanoparticle–oil interface, giving rise to the higher shallow trap density and resulting in better charge transport in SNFs.
    Keywords colloids ; relative humidity ; titanium dioxide ; oils ; electrophoresis ; adsorption ; nanoparticles ; electric field
    Language English
    Document type Article
    ISSN 0927-7757
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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