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  1. Article ; Online: Identification and Transcriptome Analysis of a Novel Allelic Mutant of

    Wang, Yang / Xu, Wanxin / Liu, Yan / Yang, Jie / Guo, Xin / Zhang, Jiaruo / Pu, Jisong / Chen, Nenggang / Zhang, Wenfeng

    Genes

    2024  Volume 15, Issue 3

    Abstract: Leaf morphology is a crucial aspect of plant architecture, yet the molecular mechanisms underlying leaf development remain incompletely understood. In this study, a narrow leaf mutant, ...

    Abstract Leaf morphology is a crucial aspect of plant architecture, yet the molecular mechanisms underlying leaf development remain incompletely understood. In this study, a narrow leaf mutant,
    MeSH term(s) Oryza ; Phenotype ; Alleles ; Cell Division ; Gene Expression Profiling
    Language English
    Publishing date 2024-03-02
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527218-4
    ISSN 2073-4425 ; 2073-4425
    ISSN (online) 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes15030325
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: A Single Nucleotide Mutation of the IspE Gene Participating in the MEP Pathway for Isoprenoid Biosynthesis Causes a Green-Revertible Yellow Leaf Phenotype in Rice

    Chen, Nenggang / Deng, Xiaojian / Li, Caixia / Li, Chunmei / Pan, Jihong / Sun, Changhui / Wang, Pingrong / Wang, Qian / Wang, Yang / Xiao, Fuliang / Yang, Bin / Zhang, Kuan

    Plant & cell physiology. 2018 Sept. 01, v. 59, no. 9

    2018  

    Abstract: Plant isoprenoids are dependent on two independent pathways, the cytosolic mevalonate (MVA) pathway and the plastidic methylerythritol phosphate (MEP) pathway. IspE is one of seven known enzymes in the MEP pathway. Currently, no IspE gene has been ... ...

    Abstract Plant isoprenoids are dependent on two independent pathways, the cytosolic mevalonate (MVA) pathway and the plastidic methylerythritol phosphate (MEP) pathway. IspE is one of seven known enzymes in the MEP pathway. Currently, no IspE gene has been identified in rice. In addition, no virescent mutants have been reported to result from a gene mutation affecting the MEP pathway. In this study, we isolated a green-revertible yellow leaf mutant gry340 in rice. The mutant exhibited a reduced level of photosynthetic pigments, and an arrested development of chloroplasts and mitochondria in its yellow leaves. Map-based cloning revealed a missense mutation in OsIspE (LOC_Os01g58790) in gry340 mutant plants. OsIspE is constitutively expressed in all tissues, and its encoded protein is targeted to the chloroplast. Further, the mutant phenotype of gry340 was rescued by introduction of the wild-type gene. Therefore, we have successfully identified an IspE gene in monocotyledons via map-based cloning, and confirmed that the green-revertible yellow leaf phenotype of gry340 does result from a single nucleotide mutation in the IspE gene. In addition, the ispE ispF double mutant displayed an etiolation lethal phenotype, indicating that the isoprenoid precursors from the cytosol cannot efficiently compensate for the deficiency of the MEP pathway in rice chloroplasts. Furthermore, real-time quantitative reverse transcription–PCR suggested that this functional defect in OsIspE affected the expression of not only other MEP pathway genes but also that of MVA pathway genes, photosynthetic genes and mitochondrial genes.
    Keywords biosynthesis ; chloroplasts ; cytosol ; enzymes ; etiolation ; gene expression ; isoprenoids ; leaves ; Liliopsida ; missense mutation ; mitochondria ; mitochondrial genes ; molecular cloning ; mutants ; phenotype ; phosphates ; photosynthesis ; pigments ; reverse transcriptase polymerase chain reaction ; rice ; tissues
    Language English
    Dates of publication 2018-0901
    Size p. 1905-1917.
    Publishing place Oxford University Press
    Document type Article
    ZDB-ID 208907-5
    ISSN 1471-9053 ; 0032-0781
    ISSN (online) 1471-9053
    ISSN 0032-0781
    DOI 10.1093/pcp/pcy108
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 79/710: Show issues

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  3. Article ; Online: A Single Nucleotide Mutation of the IspE Gene Participating in the MEP Pathway for Isoprenoid Biosynthesis Causes a Green-Revertible Yellow Leaf Phenotype in Rice.

    Chen, Nenggang / Wang, Pingrong / Li, Chunmei / Wang, Qian / Pan, Jihong / Xiao, Fuliang / Wang, Yang / Zhang, Kuan / Li, Caixia / Yang, Bin / Sun, Changhui / Deng, Xiaojian

    Plant & cell physiology

    2018  Volume 59, Issue 9, Page(s) 1905–1917

    Abstract: Plant isoprenoids are dependent on two independent pathways, the cytosolic mevalonate (MVA) pathway and the plastidic methylerythritol phosphate (MEP) pathway. IspE is one of seven known enzymes in the MEP pathway. Currently, no IspE gene has been ... ...

    Abstract Plant isoprenoids are dependent on two independent pathways, the cytosolic mevalonate (MVA) pathway and the plastidic methylerythritol phosphate (MEP) pathway. IspE is one of seven known enzymes in the MEP pathway. Currently, no IspE gene has been identified in rice. In addition, no virescent mutants have been reported to result from a gene mutation affecting the MEP pathway. In this study, we isolated a green-revertible yellow leaf mutant gry340 in rice. The mutant exhibited a reduced level of photosynthetic pigments, and an arrested development of chloroplasts and mitochondria in its yellow leaves. Map-based cloning revealed a missense mutation in OsIspE (LOC_Os01g58790) in gry340 mutant plants. OsIspE is constitutively expressed in all tissues, and its encoded protein is targeted to the chloroplast. Further, the mutant phenotype of gry340 was rescued by introduction of the wild-type gene. Therefore, we have successfully identified an IspE gene in monocotyledons via map-based cloning, and confirmed that the green-revertible yellow leaf phenotype of gry340 does result from a single nucleotide mutation in the IspE gene. In addition, the ispE ispF double mutant displayed an etiolation lethal phenotype, indicating that the isoprenoid precursors from the cytosol cannot efficiently compensate for the deficiency of the MEP pathway in rice chloroplasts. Furthermore, real-time quantitative reverse transcription-PCR suggested that this functional defect in OsIspE affected the expression of not only other MEP pathway genes but also that of MVA pathway genes, photosynthetic genes and mitochondrial genes.
    MeSH term(s) Base Sequence ; Chloroplasts/metabolism ; Cloning, Molecular ; Gene Expression Regulation, Plant ; Genetic Complementation Test ; Mitochondria/metabolism ; Oryza/genetics ; Photosynthesis/genetics ; Photosynthesis/physiology ; Phylogeny ; Plant Leaves/metabolism ; Plant Proteins/metabolism ; Polymorphism, Single Nucleotide ; Terpenes/metabolism
    Chemical Substances Plant Proteins ; Terpenes
    Language English
    Publishing date 2018-06-11
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 208907-5
    ISSN 1471-9053 ; 0032-0781
    ISSN (online) 1471-9053
    ISSN 0032-0781
    DOI 10.1093/pcp/pcy108
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 79/710: Show issues

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  4. Article ; Online: A single nucleotide mutation of IspF gene involved in the MEP pathway for isoprenoid biosynthesis causes yellow-green leaf phenotype in rice.

    Huang, Rui / Wang, Yang / Wang, Pingrong / Li, Chunmei / Xiao, Fuliang / Chen, Nenggang / Li, Na / Li, Caixia / Sun, Changhui / Li, Lihua / Chen, Rongjun / Xu, Zhengjun / Zhu, Jianqing / Deng, Xiaojian

    Plant molecular biology

    2017  Volume 96, Issue 1-2, Page(s) 5–16

    Abstract: Key message: We identified IspF gene through yellow-green leaf mutant 505ys in rice. OsIspF was expressed in all tissues detected, and its encoded protein was targeted to the chloroplast. On expression levels of genes in this mutant, OsIspF itself and ... ...

    Abstract Key message: We identified IspF gene through yellow-green leaf mutant 505ys in rice. OsIspF was expressed in all tissues detected, and its encoded protein was targeted to the chloroplast. On expression levels of genes in this mutant, OsIspF itself and the genes encoding other enzymes of the MEP pathway and chlorophyll synthase were all up-regulated, however, among eight genes associated with photosynthesis, only psaA, psaN and psbA genes for three reaction center subunits of photosystem obviously changed. Isoprenoids are the most abundant natural compounds in all organisms, which originate from the basic five-carbon units isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). In plants, IPP and DMAPP are synthesized through two independent pathways, the mevalonic acid pathway in cytoplasm and the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway in plastids. The MEP pathway comprises seven enzymatic steps, in which IspF is the fifth enzyme. So far, no IspF gene has been identified in monocotyledonous plants. In this study, we isolated a leaf-color mutant, 505ys, in rice (Oryza sativa). The mutant displayed yellow-green leaf phenotype, reduced level of photosynthetic pigments, and arrested development of chloroplasts. By map-based cloning of this mutant, we identified OsIspF gene (LOC_Os02g45660) showing significant similarity to IspF gene of Arabidopsis, in which a missense mutation occurred in the mutant, resulting in an amino acid change in the encoded protein. OsIspF gene was expressed in all tissues detected, and its encoded protein was targeted to the chloroplast. Further, the mutant phenotype of 505ys was complemented by transformation with the wild-type OsIspF gene. Therefore, we successfully identified an IspF gene in monocotyledonous plants. In addition, real-time quantitative RT-PCR implied that a positive regulation could exist between the OsIspF gene and the genes encoding other enzymes of the MEP pathway and chlorophyll synthase. At the same time, it also implied that the individual genes involved in the MEP pathway might differentially regulated expression levels of the genes associated with photosynthesis.
    MeSH term(s) Erythritol/analogs & derivatives ; Gene Expression Regulation, Plant/genetics ; Gene Expression Regulation, Plant/physiology ; Hemiterpenes/metabolism ; Mutation/genetics ; Organophosphorus Compounds/metabolism ; Oryza/genetics ; Oryza/metabolism ; Phenotype ; Plant Diseases/genetics ; Plant Leaves/genetics ; Plant Leaves/metabolism ; Plant Proteins/genetics ; Plant Proteins/physiology ; Sugar Phosphates ; Terpenes/metabolism
    Chemical Substances 2-C-methylerythritol 4-phosphate ; Hemiterpenes ; Organophosphorus Compounds ; Plant Proteins ; Sugar Phosphates ; Terpenes ; isopentenyl pyrophosphate (358-71-4) ; Erythritol (RA96B954X6)
    Language English
    Publishing date 2017-11-15
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 778032-1
    ISSN 1573-5028 ; 0167-4412
    ISSN (online) 1573-5028
    ISSN 0167-4412
    DOI 10.1007/s11103-017-0668-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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