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Article: Analysis of carbapenem-resistant

Wang, Lin / Chen, Qi-Wei / Qin, Yan-Chun / Yi, Xue-Li / Zeng, Hong

International journal of molecular epidemiology and genetics

2024 Volume 15, Issue 1, Page(s) 1–11

Abstract: Objective: In recent years, : Methods: The clinically isolated strains were detected using Vitek mass spectrometry and Vitek2-compact for bacterial identification and susceptibility testing, respectively. The biofilms of clinical isolates were ... ...

Abstract	Objective: In recent years, Methods: The clinically isolated strains were detected using Vitek mass spectrometry and Vitek2-compact for bacterial identification and susceptibility testing, respectively. The biofilms of clinical isolates were quantitatively detected by microplate crystal violet staining, and qualitatively observed by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). And the common carbapenemase genes were detected by polymerase chain reaction (PCR). Results: The 255 clinical isolates from the Youjiang District of western Guangxi Province had a high resistance rate to carbapenems antibiotics. The main specimens were from the intensive care unit (49%), and the most important specimens were sputum specimens (80%). All 145 strains of CRAB produced different degrees of biofilm, and six carbapenenase genes were detected. We found that there were significant differences in biofilm formation between resistant and sensitive strains of tobramycin, levofloxacin, ciprofloxacin, tigecycline, and doxycycline ( Conclusion: The clinical strains have a high resistance rate to carbapenems, and the CRAB with
Language	English
Publishing date	2024-02-15
Publishing country	United States
Document type	Journal Article
ZDB-ID	2553441-5
ISSN	1948-1756
ISSN	1948-1756
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: A Serious Game ("Fight With Virus") for Preventing COVID-19 Health Rumors: Development and Experimental Study.

Xiong, Shuo / Zuo, Long / Chen, Qiwei / Zeliang, Zhang / Nor Akmal Khalid, Mohd

JMIR serious games

2024 Volume 12, Page(s) e45546

Abstract: Background: Health rumors arbitrarily spread in mainstream social media on the internet. Health rumors emerged in China during the outbreak of COVID-19 in early 2020. Many midelders/elders (age over 40 years) who lived in Wuhan believed these rumors.: ...

Abstract	Background: Health rumors arbitrarily spread in mainstream social media on the internet. Health rumors emerged in China during the outbreak of COVID-19 in early 2020. Many midelders/elders (age over 40 years) who lived in Wuhan believed these rumors. Objective: This study focused on designing a serious game as an experimental program to prevent and control health rumors. The focus of the study was explicitly on the context of the social networking service for midelders/elders. Methods: This research involved 2 major parts: adopting the Transmission Control Protocol model for games and then, based on the model, designing a game named "Fight With Virus" as an experimental platform and developing a cognitive questionnaire with a 5-point Likert scale. The relevant variables for this experimental study were defined, and 10 hypotheses were proposed and tested with an empirical study. In total, 200 participants were selected for the experiments. By collecting relevant data in the experiments, we conducted statistical observations and comparative analysis to test whether the experimental hypotheses could be proved. Results: We noted that compared to traditional media, serious games are more capable of inspiring interest in research participants toward their understanding of the knowledge and learning of health commonsense. In judging and recognizing the COVID-19 health rumor, the test group that used game education had a stronger ability regarding identification of the rumor and a higher accuracy rate of identification. Results showed that the more educated midelders/elders are, the more effective they are at using serious games. Conclusions: Compared to traditional media, serious games can effectively improve midelders'/elders' cognitive abilities while they face a health rumor. The gameplay effect is related to the individual's age and educational background, while income and gender have no impact.
Language	English
Publishing date	2024-02-26
Publishing country	Canada
Document type	Journal Article
ZDB-ID	2798265-8
ISSN	2291-9279
ISSN	2291-9279
DOI	10.2196/45546
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Article: Adsorption film with sub-milli-interface morphologies via direct ink writing for indoor formaldehyde removal

Chen, Qiwei / Tian, Enze / Luo, Ziyi / Mo, Jinhan

Journal of hazardous materials. 2022 Apr. 05, v. 427

2022

Abstract: In-situ thermally regenerated flexible adsorption films are superior for long-term purification of indoor low-concentration volatile organic compounds (VOCs). To further improve the adsorption kinetics of the films, the surface morphology of adsorption ... ...

Abstract	In-situ thermally regenerated flexible adsorption films are superior for long-term purification of indoor low-concentration volatile organic compounds (VOCs). To further improve the adsorption kinetics of the films, the surface morphology of adsorption films was suggested in hierarchical channel structure. However, such structure is far from practical applications because of its complicated fabrication method and limited flexibility. In this study, we proposed a convenient and fast method named direct ink writing (DIW) based 3D printing to fabricate flexible adsorption films. Inks were prepared to have appropriate rheological properties and good printability. Three types of adsorption film (flat, straight finned, and trough-like finned) were constructed on flexible polyimide circuit substrates by DIW. We utilized the printed adsorption films for indoor level (1 ppm) formaldehyde removal. The trough-like finned film achieved the best performance among the three printed films, showing a 275% longer penetration time and 252% larger effective adsorption capacity than the flat film. By conducting a 7-cycle adsorption-desorption experiment (more than 12 h), we verified that the films’ adsorption performance could effectively recover via in-situ heating. This work could dance around the complicated coating process, increase the structural flexibility and reduce the adsorbent interfacial modification cost.
Keywords	adsorbents ; adsorption ; formaldehyde ; heat ; volatile organic compounds
Language	English
Dates of publication	2022-0405
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1491302-1
ISSN	1873-3336 ; 0304-3894
ISSN (online)	1873-3336
ISSN	0304-3894
DOI	10.1016/j.jhazmat.2021.128190
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Article: Effect of adjuvant radiotherapy after breast-conserving surgery in elder women with early-stage breast cancer: a propensity-score matching analysis.

Han, Tingting / Shi, Mingwei / Chen, Qiwei / Chen, Dongbo / Hao, Jiqing

Frontiers in oncology

2023 Volume 13, Page(s) 1012139

Abstract: Purpose: The study aimed to explore the role of adjuvant radiotherapy (RT) after breast-conserving surgery (BCS) in elder women with early-stage breast cancer (BC).: Methods: BC patients with 70-79 years of age, stage T1-2N0-1M0, undergoing BCS were ... ...

Abstract	Purpose: The study aimed to explore the role of adjuvant radiotherapy (RT) after breast-conserving surgery (BCS) in elder women with early-stage breast cancer (BC). Methods: BC patients with 70-79 years of age, stage T1-2N0-1M0, undergoing BCS were screened in the Surveillance, Epidemiology and End Results (SEER) database between 2010 and 2015. The clinicopathological characteristics were balanced with propensity-score matching (PSM) method. Kaplan-Meier curves and Cox regression analyses were performed to determine the impact of adjuvant RT on BC patients. Results: Ultimately, 12,310 patients treated with adjuvant RT and 4837 patients treated with no RT, were involved in the analysis. Overall, patients treated with adjuvant RT was associated with a better breast cancer-specific survival (BCSS) (HR: 1.980 [1.596- 2.456], P < 0.001) and overall survival (OS) (HR: 2.214 [1.966- 2.494], P < 0.001) than those who did not undergo RT. After 1:1 PSM, adjuvant RT still performed advantage in both BCSS (HR: 1.918 [1.439- 2.557], P < 0.001) and OS (HR: 2.235 [1.904- 2.624], P < 0.001). In the multivariate COX analysis of BCSS, widowed, divorced and separated patients, tumor grade III, T2 stage, N1 stage, no RT, molecular subtypes with luminal B and triple negative were associated with a shorter BCSS (P < 0.05). In the multivariate COX analysis of OS, age ≥74 years, widowed, divorced and separated patients, tumor grade II/III, T2 stage, no RT, no chemotherapy, molecular subtypes with triple negative were associated with a shorter OS (P < 0.05). Furthermore, the advantages of adjuvant RT were observed in all subgroup analysis. Conclusion: Adjuvant RT after BCS can improve both BCSS and OS in elderly patients with early-stage BC. Additionally, all subgroups analysis-derived BCSS and OS were in support of RT.
Language	English
Publishing date	2023-10-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2649216-7
ISSN	2234-943X
ISSN	2234-943X
DOI	10.3389/fonc.2023.1012139
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Article: ARID1A promotes chemosensitivity to gemcitabine in pancreatic cancer through epigenetic silencing of RRM2.

Li, Wenyi / Chen, Qiwei / Gao, Weiwei / Zeng, Hui

Die Pharmazie

2022 Volume 77, Issue 7, Page(s) 224–229

Abstract: Pancreatic cancer is one of the most common malignancies with very poor prognosis due to its broad resistance to chemotherapy. ARID1A, a subunit of SWI/SNF complex, is involved in pancreatic carcinogenesis through epigenetic silencing of oncogenes. In ... ...

Abstract	Pancreatic cancer is one of the most common malignancies with very poor prognosis due to its broad resistance to chemotherapy. ARID1A, a subunit of SWI/SNF complex, is involved in pancreatic carcinogenesis through epigenetic silencing of oncogenes. In this study, we aimed to explore whether ARID1A was implicated in the gemcitabine resistance in pancreatic cancer patients via regulating RRM2. We examined the effect of ARID1A depletion on the gemcitabine sensitivity in pancreatic cancer cells and explored the role of RRM2 in ARID1A-mediated pancreatic cancer cells chemosensitivity to gemcitabine. We found that Knockout of ARID1A led to gemcitabine resistance in pancreatic cancer cells, effect of which could be reversed by RRM2, a gemcitabine resistance related gene. ARID1A decreased the transcription of RRM2, and directly bound to the promoter of RRM2. Moreover, expression of RRM2 was negatively correlated with ARID1A in pancreatic cancer tissues. Thus, ARID1A-mediated RRM2 epigenetic suppression is crucial for enhancement of pancreatic cancer chemosensitivity to gemcitabine, and ARID1A could be used as a biomarker to guide the gemcitabine chemotherapy of pancreatic cancer.
MeSH term(s)	Cell Line, Tumor ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Deoxycytidine/analogs & derivatives ; Deoxycytidine/pharmacology ; Epigenesis, Genetic/genetics ; Humans ; Pancreatic Neoplasms/drug therapy ; Pancreatic Neoplasms/genetics ; Ribonucleoside Diphosphate Reductase/genetics ; Ribonucleoside Diphosphate Reductase/metabolism ; Transcription Factors/genetics ; Gemcitabine ; Pancreatic Neoplasms
Chemical Substances	ARID1A protein, human ; DNA-Binding Proteins ; Transcription Factors ; Deoxycytidine (0W860991D6) ; ribonucleotide reductase M2 (EC 1.17.4.-) ; Ribonucleoside Diphosphate Reductase (EC 1.17.4.1) ; Gemcitabine
Language	English
Publishing date	2022-10-05
Publishing country	Germany
Document type	Journal Article
ZDB-ID	208793-5
ISSN	0031-7144
ISSN	0031-7144
DOI	10.1691/ph.2022.1881
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Book ; Online: Entire Space Learning Framework

Lyu, Shanshan / Chen, Qiwei / Zhuang, Tao / Ge, Junfeng

Unbias Conversion Rate Prediction in Full Stages of Recommender System

2023

Abstract: Recommender system is an essential part of online services, especially for e-commerce platform. Conversion Rate (CVR) prediction in RS plays a significant role in optimizing Gross Merchandise Volume (GMV) goal of e-commerce. However, CVR suffers from ... ...

Abstract	Recommender system is an essential part of online services, especially for e-commerce platform. Conversion Rate (CVR) prediction in RS plays a significant role in optimizing Gross Merchandise Volume (GMV) goal of e-commerce. However, CVR suffers from well-known Sample Selection Bias (SSB) and Data Sparsity (DS) problems. Although existing methods ESMM and ESM2 train with all impression samples over the entire space by modeling user behavior paths, SSB and DS problems still exist. In real practice, the online inference space are samples from previous stage of RS process, rather than the impression space modeled by existing methods. Moreover, existing methods solve the DS problem mainly by building behavior paths of their own specific scene, ignoring the behaviors in various scenes of e-commerce platform. In this paper, we propose Entire Space Learning Framework: Unbias Conversion Rate Prediction in Full Stages of Recommender System, solving SSB and DS problems by reformulating GMV goal in a novel manner. Specifically, we rebuild the CVR on the entire data space with samples from previous stage of RS process, unifying training and online inference space. Moreover, we explicitly introduce purchase samples from other scenes of e-commerce platform in model learning process. Online A/B test and offline experiments show the superiority of our framework. Our framework has been deployed in rank stage of Taobao recommendation, providing recommendation service for hundreds of millions of consumers everyday.
Keywords	Computer Science - Information Retrieval
Subject code	004
Publishing date	2023-03-01
Publishing country	us
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: OmpA is involved in the invasion of duck brain microvascular endothelial cells by Riemerella anatipestifer

Zou, Ronghua / Wu, Xiaoni / Chen, Qiwei / Gong, Xiaowei / Chu, Yuefeng / Zheng, Fuying

Veterinary Microbiology. 2023 May, v. 280 p.109692-

2023

Abstract: Bacterial meningitis is a major cause of morbidity and mortality. Despite advances in antimicrobial chemotherapy, the disease remains detrimental to humans, livestock, and poultry. Riemerella anatipestifer is a gram-negative bacterium causing duckling ... ...

Abstract	Bacterial meningitis is a major cause of morbidity and mortality. Despite advances in antimicrobial chemotherapy, the disease remains detrimental to humans, livestock, and poultry. Riemerella anatipestifer is a gram-negative bacterium causing duckling serositis and meningitis. However, the virulence factors contributing to its binding and invasion of duck brain microvascular endothelial cells (DBMECs) and penetration of the blood-brain barrier (BBB) have never been reported. In this study, immortalized DBMECs were successfully generated and used as an in vitro-model of duck BBB. Furthermore, ompA gene deletion mutant of the pathogen and multiple complemented strains carrying the complete ompA gene and its truncated forms were constructed. Bacterial growth, invasion, and adhesion assays and animal experiments were performed. The results show that the OmpA protein of R. anatipestifer had no effect on bacterial growth and adhesion ability to DBMECs. The role of OmpA in the invasion of R. anatipestifer into DBMECs and duckling BBB was confirmed. The amino acids 230-242 of OmpA represents a key domain involved in R. anatipestifer invasion. In addition, another OmpA1164 protein constituted by the amino acids 102-488 within OmpA could function as a complete OmpA. The signal peptide sequence from amino acids 1-21 had no significant effect on OmpA functions. In conclusion, this study illustrated that OmpA is an important virulence factor mediating R. anatipestifer invasion of DBMECs and penetration of the duckling BBB.
Keywords	Gram-negative bacteria ; Riemerella anatipestifer ; adhesion ; bacterial growth ; blood-brain barrier ; brain ; drug therapy ; ducklings ; gene deletion ; genes ; meningitis ; morbidity ; mortality ; mutants ; pathogens ; signal peptide ; virulence ; Duck brain microvascular endothelial cells ; Blood brain barrier ; OmpA protein
Language	English
Dates of publication	2023-05
Publishing place	Elsevier B.V.
Document type	Article ; Online
ZDB-ID	753154-0
ISSN	1873-2542 ; 0378-1135
ISSN (online)	1873-2542
ISSN	0378-1135
DOI	10.1016/j.vetmic.2023.109692
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: A phase separation process induced by pH change for purification of His-tagged protein at low salt concentration

Wang, Hongrui / Sun, Hongxu / Gao, Chao / Chen, Qiwei / Dong, Wenge / Chang, Yanhong / Luo, Hui

Biochemical Engineering Journal. 2023 Feb., v. 191 p.108792-

2023

Abstract: Protein purification is critical for protein research and enzyme manufacture, and efficient purification methods with low cost have a high priority. In this study, the difference in solubility between His-tagged protein and nontagged protein led to a ... ...

Abstract	Protein purification is critical for protein research and enzyme manufacture, and efficient purification methods with low cost have a high priority. In this study, the difference in solubility between His-tagged protein and nontagged protein led to a liquid-solid phase separation at neutral pH and low salt concentration and purified His-tagged protein to some extent. The investigation of impurity in the precipitate indicated that solvation behavior varied from several oligo-amino acid tagged proteins. Among them, proteins with oligo-Lys, oligo-Trp and oligo-Arg obviously precipitated at low salt concentration and weak acidic pH, which is similar to that for the precipitation of His-tagged protein. Further study suggested that proteins with oligo-Lys, oligo-Trp and oligo-Arg remained a precipitate state while His-tagged protein dissolved at a slightly higher pH value. Based on these finding, a method was developed for the purification of His-tagged protein via phase separation induced by pH (PSIP) at low salt concentration. Though the yield and purity of protein purified by PSIP were slightly inferior to that by immobilized metal ion affinity chromatography (IMAC), PSIP was a high-throughput and low-cost method for purification of His-tagged proteins. Beyond the purification process, the various solvation behaviors of proteins with different oligo-amino acid also provide a reference for solvation research of protein.
Keywords	affinity chromatography ; enzymes ; manufacturing ; pH ; purification methods ; salt concentration ; separation ; solubility ; solvation ; Protein purification ; Phase separation ; Histidine tag ; Solvation behavior
Language	English
Dates of publication	2023-02
Publishing place	Elsevier B.V.
Document type	Article ; Online
ZDB-ID	2012139-8
ISSN	1369-703X
ISSN	1369-703X
DOI	10.1016/j.bej.2022.108792
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Effect of SpyTag/SpyCatcher cyclization on reactivation of covalently immobilized biocatalysts

Li, Ren / Song, Haiyan / Chen, Qiwei / Sun, Hongxu / Chang, Yanhong / Luo, Hui

Process Biochemistry. 2023 Mar., v. 126 p.87-97

2023

Abstract: SpyTag and SpyCatcher can rapidly conjugate covalently to form an irreversible covalent bond. Fusing SpyTag and SpyCatcher to the termini of a protein of interest enables the generation of SpyRing structure, which is often helpful for the stability of ... ...

Abstract	SpyTag and SpyCatcher can rapidly conjugate covalently to form an irreversible covalent bond. Fusing SpyTag and SpyCatcher to the termini of a protein of interest enables the generation of SpyRing structure, which is often helpful for the stability of protein. In this paper, green fluorescent protein (GFP), L-phenylserine aldolase (LPA) and SRGFP, SRLPA (their SpyRing cyclized form) were covalently bound to glyoxyl-agarose (Gx) beads to prepare immobilized enzymes (Gx-GFP, Gx-LPA, Gx-SRGFP and Gx-SRLPA) with different protein loadings, and the effects of the SpyRing structure on solid-phase refolding were investigated. When the immobilized enzyme was inactivated in high concentrations of N, N-dimethylformamide (DMF) and 9 M guanidine hydrochloride, then reactivated with refolding buffer, the results showed that proteins with the SpyRing structure had a higher renaturation yield than their wild-type counterparts in all three replication cycles. The highest renaturation yields of Gx-SRGFP and Gx-SRLPA were over 90 % and 70 % respectively, which were approximately 15 % and 30 % higher than those of Gx-GFP and Gx-LPA. It suggests that the introduction of the SpyRing structure facilitates the reactivation of immobilized biocatalysts and provides a reference for the regeneration and recycling of industrial biocatalysts.
Keywords	chemical bonding ; fructose-bisphosphate aldolase ; green fluorescent protein ; guanidines ; immobilized enzymes ; SpyTag/SpyCatcher ; Covalent immobilization ; Inactivation ; Reactivation ; Renaturation yields ; Protein loading
Language	English
Dates of publication	2023-03
Size	p. 87-97.
Publishing place	Elsevier Ltd
Document type	Article ; Online
ISSN	1359-5113
DOI	10.1016/j.procbio.2023.01.005
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Article: Vertical macro-channel modification of a flexible adsorption board with in-situ thermal regeneration for indoor gas purification to increase effective adsorption capacity

Chen, Qiwei / Liu, Fang / Mo, Jinhan

Environmental research. 2021 Jan., v. 192

2021

Abstract: Adsorption has been used widely to remove indoor volatile organic compounds (VOCs). However, the large diffusion resistance inside traditional granular adsorbents renders a low VOC adsorption rate. This study proposes a modified method to achieve the ... ...

Abstract	Adsorption has been used widely to remove indoor volatile organic compounds (VOCs). However, the large diffusion resistance inside traditional granular adsorbents renders a low VOC adsorption rate. This study proposes a modified method to achieve the rapid diffusion into the adsorbent during the initial adsorption period. A thin and flexible adsorption board with a layer of adsorbent coated on a heating film was prepared for in-situ adsorption and regeneration. Then, regular, vertical macro-channels through the adsorption board were fabricated by laser drilling to enhance mass transfer inside the board. Experimental results demonstrated that after modification, the penetration times for formaldehyde and xylene extended from 3.8 to 6.2 h, and from 62 to 99 h, respectively. The effective adsorption capacity of the modified board had increased by a multiple of two for formaldehyde and 1.8 for xylene. A mathematical model was developed and experimentally validated to evaluate the modification effect for more adsorbent-pollutant pairs. The results showed that the amplification of effective adsorption capacity was positively correlated with the Da/(K·De) parameter; this is the diffusion resistance ratio prior to and following the modification. A spectrogram of adsorbent–pollutant pairs was plotted to guide the modification. This simple macro-channel modification of the adsorption board may be used as an alternative design for adsorption applications in indoor air purification.
Keywords	adsorbents ; adsorption ; air ; formaldehyde ; mass transfer ; mathematical models ; research ; volatile organic compounds ; xylene
Language	English
Dates of publication	2021-01
Publishing place	Elsevier Inc.
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	205699-9
ISSN	1096-0953 ; 0013-9351
ISSN (online)	1096-0953
ISSN	0013-9351
DOI	10.1016/j.envres.2020.110218
Database	NAL-Catalogue (AGRICOLA)

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