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Article ; Online: Knockout of cryptochrome 1 disturbs the locomotor circadian rhythm and development of Plutella xylostella

Chen, Shao‐Ping / Wang, Dan‐Feng / Ma, Wei‐Feng / Lin, Xiao‐Lu / Yang, Guang

Insect Science. 2023 Aug., v. 30, no. 4 p.1035-1045

2023

Abstract: Cryptochrome 1 (CRY1) functions as a light‐responsive photoreceptor, which is crucial for circadian rhythms. The identity and function of CRY1 in Plutella xylostella remain unknown. In this study, cry1 was cloned and identified in P. xylostella. Then, a ... ...

Abstract	Cryptochrome 1 (CRY1) functions as a light‐responsive photoreceptor, which is crucial for circadian rhythms. The identity and function of CRY1 in Plutella xylostella remain unknown. In this study, cry1 was cloned and identified in P. xylostella. Then, a cry1‐knockout strain (Cry1‐KO) of P. xylostella with a 2‐bp deletion was established from the strain Geneva 88 (G88) using the CRISPR/Cas9 technology. No daily temporal oscillation of cry1 was observed in G88 and Cry1‐KO, and cry1 mean daily transcription of Cry1‐KO was lower than that of G88. Both G88 and Cry1‐KO demonstrated rhythmic locomotion under the light/dark condition with Cry1‐KO being more active than G88 in the daytime, whereas Cry1‐KO completely lost rhythmicity under constant darkness. The developmental period of pre‐adult of Cry1‐KO was longer than that of G88; the lifespan of the Cry1‐KO male adult was shorter than that of G88; the fecundity of Cry1‐KO was lower than that of G88; and Cry1‐KO showed lower intrinsic rate of increase (r), net reproduction rate (R₀), finite increase rate (λ), and longer mean generation time (T) than G88. Our results indicate that cry1 is involved in the regulation of locomotor circadian rhythm and development in P. xylostella, providing a potential target gene for controlling the pest and a basis for further investigation on circadian rhythms in lepidopterans.
Keywords	CRISPR-Cas systems ; Plutella xylostella ; adults ; circadian rhythm ; cryptochromes ; fecundity ; genes ; insects ; locomotion ; longevity ; males ; pests ; photoreceptors
Language	English
Dates of publication	2023-08
Size	p. 1035-1045.
Publishing place	John Wiley & Sons, Ltd
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	2179775-4
ISSN	1744-7917 ; 1672-9609
ISSN (online)	1744-7917
ISSN	1672-9609
DOI	10.1111/1744-7917.13150
Database	NAL-Catalogue (AGRICOLA)

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Article: An

Chen, Shao-Ping / Lin, Xiao-Lu / Qiu, Rong-Zhou / Chi, Mei-Xiang / Yang, Guang

Insects

2023 Volume 14, Issue 1

Abstract: ... Plutella ... ...

Abstract	Plutella xylostella
Language	English
Publishing date	2023-01-12
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662247-6
ISSN	2075-4450
ISSN	2075-4450
DOI	10.3390/insects14010072
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Article ; Online: Knockout of cryptochrome 1 disturbs the locomotor circadian rhythm and development of Plutella xylostella.

Chen, Shao-Ping / Wang, Dan-Feng / Ma, Wei-Feng / Lin, Xiao-Lu / Yang, Guang

Insect science

2022 Volume 30, Issue 4, Page(s) 1035–1045

Abstract: Cryptochrome 1 (CRY1) functions as a light-responsive photoreceptor, which is crucial for circadian rhythms. The identity and function of CRY1 in Plutella xylostella remain unknown. In this study, cry1 was cloned and identified in P. xylostella. Then, a ... ...

Abstract	Cryptochrome 1 (CRY1) functions as a light-responsive photoreceptor, which is crucial for circadian rhythms. The identity and function of CRY1 in Plutella xylostella remain unknown. In this study, cry1 was cloned and identified in P. xylostella. Then, a cry1-knockout strain (Cry1-KO) of P. xylostella with a 2-bp deletion was established from the strain Geneva 88 (G88) using the CRISPR/Cas9 technology. No daily temporal oscillation of cry1 was observed in G88 and Cry1-KO, and cry1 mean daily transcription of Cry1-KO was lower than that of G88. Both G88 and Cry1-KO demonstrated rhythmic locomotion under the light/dark condition with Cry1-KO being more active than G88 in the daytime, whereas Cry1-KO completely lost rhythmicity under constant darkness. The developmental period of pre-adult of Cry1-KO was longer than that of G88; the lifespan of the Cry1-KO male adult was shorter than that of G88; the fecundity of Cry1-KO was lower than that of G88; and Cry1-KO showed lower intrinsic rate of increase (r), net reproduction rate (R
MeSH term(s)	Male ; Animals ; Cryptochromes/genetics ; Circadian Rhythm/genetics ; Transcription Factors
Chemical Substances	Cryptochromes ; Transcription Factors
Language	English
Publishing date	2022-12-26
Publishing country	Australia
Document type	Journal Article
ZDB-ID	2179775-4
ISSN	1744-7917 ; 1672-9609
ISSN (online)	1744-7917
ISSN	1672-9609
DOI	10.1111/1744-7917.13150
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Student-Teacher Relationship: Its Measurement and Effect on Students' Trait, Performance, and Wellbeing in Private College.

Bai, Li Ying / Li, Zi Ying / Wu, Wen Xin / Liu, Li Yue / Chen, Shao Ping / Zhang, Jing / Zhu, Julie N Y

Frontiers in psychology

2022 Volume 13, Page(s) 793483

Abstract: Student-teacher relationships (STRs) have been examined by many studies. However, an omission still exists, the existing scales are not appropriate for studying STRs in private colleges because of the special character of these schools. This paper ... ...

Abstract	Student-teacher relationships (STRs) have been examined by many studies. However, an omission still exists, the existing scales are not appropriate for studying STRs in private colleges because of the special character of these schools. This paper presents the development and validation of Private-College Student-Teacher Relationship Scale (PCSTRS), the first instrument to evaluate student-teacher relationships (STRs) in private colleges. The PCSTRS has six dimensions: trust, interaction, intimacy, care, approval, and comfort. In our main study, the validity and reliability of the six-factor PCSTRS model were demonstrated. The result of internal consistency coefficient indicated the high reliability of the scale, and the result of concurrent validity indicated the significant correlational relationships between the PCSTRS with other STR measures. In supplementary study, the PCSTRS was administered to 360 participants to confirm the applicability of PCSTRS and investigate the relation of STRs and students' traits, performance, and wellbeing, as well as the differences between the private school and the public school in this relation; the analyses revealed that there were significant differences in trust, intimacy, and care between private and public colleges; positive correlations were found between STRs and self-esteem, self-efficacy, academic performance, extracurricular activity involvement, and subjective wellbeing. Present research firstly develops the PCSTRS, examined the reliability and validity, and provides the proposed nomological network among related constructs.
Language	English
Publishing date	2022-03-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2563826-9
ISSN	1664-1078
ISSN	1664-1078
DOI	10.3389/fpsyg.2022.793483
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Article ; Online: Dysregulation of HULC promotes contrast-induced nephropathy (CIN) via regulating signaling pathway of miRNA-512 and prostaglandin E1 (PGE

Zhang, Liang / Li, Pan / Zhang, Bi-Li / Yu, Man-Li / Xu, Rong-Liang / Wu, Hong / Chen, Shao-Ping

Scientific reports

2020 Volume 10, Issue 1, Page(s) 11691

Abstract: It has been shown that contrast-induced nephropathy (CIN) can be attenuated by the administration of PGE1. As an enzyme responsible for the production of PGE1, PTGS1 was confirmed in this study as a miR-512 target. Meanwhile, HULC has been identified as ... ...

Abstract	It has been shown that contrast-induced nephropathy (CIN) can be attenuated by the administration of PGE1. As an enzyme responsible for the production of PGE1, PTGS1 was confirmed in this study as a miR-512 target. Meanwhile, HULC has been identified as a competing endogenous RNA of miR-512. Therefore, in this study, we tested the diagnostic value of HULC and miR-512 in subjects with or without CIN. In addition, we evaluated the regulatory relationship among HULC, miR-512, PTGS1 and PGE1 in vitro. We enrolled 320 patients with coronary heart disease and divided them into a CIN group and a non-CIN group. Subsequently, we detected the differential expression of miR-512, HULC and PGE1 in the two groups. We also used a dual luciferase reporter assay to evaluate the regulatory relationship among HULC, miR-512, PTGS1 and PGE1 in THP-1 cells. In patients with CIN, the expression levels of HULC and PGE1 were lower, but the expression level of miR-512 was higher. MiR-512 could directly bind to and negatively regulate the expression of PTGS1 and HULC. The expression of HULC was positively correlated with the expression of PTGS1 and PGE1, while negatively correlated with the expression of miR-512. The findings of this study demonstrated that deregulation of lncRNA-HULC/miR-512/PTGS1/PGE1 might be involved in the pathogenesis of CIN.
MeSH term(s)	Aged ; Alprostadil/metabolism ; Apoptosis/genetics ; Contrast Media/adverse effects ; Coronary Angiography/methods ; Coronary Disease/surgery ; Cyclooxygenase 1/genetics ; Cyclooxygenase 1/metabolism ; Female ; Humans ; Kidney Diseases/chemically induced ; Kidney Diseases/metabolism ; Male ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Middle Aged ; Percutaneous Coronary Intervention/methods ; RNA, Long Noncoding/genetics ; RNA, Long Noncoding/metabolism ; Signal Transduction/genetics ; THP-1 Cells ; Transfection
Chemical Substances	Contrast Media ; HULC long non-coding RNA, human ; MIRN512 microRNA, human ; MicroRNAs ; RNA, Long Noncoding ; Cyclooxygenase 1 (EC 1.14.99.1) ; PTGS1 protein, human (EC 1.14.99.1) ; Alprostadil (F5TD010360)
Language	English
Publishing date	2020-07-16
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-020-68634-7
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Article: CRISPR/Cas9‐mediated knockout of LW‐opsin reduces the efficiency of phototaxis in the diamondback moth Plutella xylostella

Chen, Shao‐Ping / Liu, Zhao‐Xia / Chen, Yan‐Ting / Wang, Yue / Chen, Jin‐Zhi / Fu, Shu / Ma, Wei‐Feng / Xia, Shuang / Liu, Dan / Wu, Tong / Yang, Guang

Pest management science. 2021 July, v. 77, no. 7

2021

Abstract: BACKGROUND: Opsins are crucial for animal vision. The identity and function of opsins in Plutella xylostella remain unknown. The aim of the research is to confirm which opsin gene(s) contribute to phototaxis of P. xylostella. RESULTS: LW‐opsin, BL‐opsin ... ...

Abstract	BACKGROUND: Opsins are crucial for animal vision. The identity and function of opsins in Plutella xylostella remain unknown. The aim of the research is to confirm which opsin gene(s) contribute to phototaxis of P. xylostella. RESULTS: LW‐opsin, BL‐opsin and UV‐opsin, were identified in the P. xylostella genome. LW‐opsin was more highly expressed than the other two opsin genes, and all three genes were specifically expressed in the head. Three P. xylostella strains, LW‐13 with a 13‐bp deletion in LW‐opsin, BL + 2 with a 2‐bp insertion in BL‐opsin, and UV‐29 with a 5‐bp insertion and a 34‐bp deletion in UV‐opsin, were established from the strain G88 using the CRISPR/Cas9 system. Among the three opsin‐knockout strains, only male and female LW‐13 exhibited weaker phototaxis to lights of different wavelengths and white light than G88 at 2.5 lx due to defective locomotion, and LW‐13 was defective to sense white, green and infrared lights. The locomotion of LW‐13 was reduced compared with G88 at 2.5, 10, 20, 60, 80, 100, and 200 lx under the green light, but the locomotion of LW‐13 female was recovered at 80, 100 and 200 lx. The defective phototaxis to the green light of male LW‐13 was not affected by light intensity, while the defective phototaxis to the green light of female LW‐13 was recovered at 10, 20, 60, 80, 100, and 200 lx. CONCLUSION: LW‐opsin is involved in light sensing and locomotion of P. xylostella, providing a potential target gene for controlling the pest. © 2021 Society of Chemical Industry.
Keywords	CRISPR-Cas systems ; Plutella xylostella ; animals ; females ; genes ; green light ; head ; light intensity ; locomotion ; males ; opsin ; pest management ; pests ; phototaxis ; vision ; white light
Language	English
Dates of publication	2021-07
Size	p. 3519-3528.
Publishing place	John Wiley & Sons, Ltd.
Document type	Article
Note	NAL-AP-2-clean ; JOURNAL ARTICLE
ZDB-ID	2001705-4
ISSN	1526-4998 ; 1526-498X
ISSN (online)	1526-4998
ISSN	1526-498X
DOI	10.1002/ps.6405
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: CRISPR/Cas9-mediated knockout of LW-opsin reduces the efficiency of phototaxis in the diamondback moth Plutella xylostella.

Chen, Shao-Ping / Liu, Zhao-Xia / Chen, Yan-Ting / Wang, Yue / Chen, Jin-Zhi / Fu, Shu / Ma, Wei-Feng / Xia, Shuang / Liu, Dan / Wu, Tong / Yang, Guang

Pest management science

2021 Volume 77, Issue 7, Page(s) 3519–3528

Abstract: Background: Opsins are crucial for animal vision. The identity and function of opsins in Plutella xylostella remain unknown. The aim of the research is to confirm which opsin gene(s) contribute to phototaxis of P. xylostella.: Results: LW-opsin, BL- ... ...

Abstract	Background: Opsins are crucial for animal vision. The identity and function of opsins in Plutella xylostella remain unknown. The aim of the research is to confirm which opsin gene(s) contribute to phototaxis of P. xylostella. Results: LW-opsin, BL-opsin and UV-opsin, were identified in the P. xylostella genome. LW-opsin was more highly expressed than the other two opsin genes, and all three genes were specifically expressed in the head. Three P. xylostella strains, LW-13 with a 13-bp deletion in LW-opsin, BL + 2 with a 2-bp insertion in BL-opsin, and UV-29 with a 5-bp insertion and a 34-bp deletion in UV-opsin, were established from the strain G88 using the CRISPR/Cas9 system. Among the three opsin-knockout strains, only male and female LW-13 exhibited weaker phototaxis to lights of different wavelengths and white light than G88 at 2.5 lx due to defective locomotion, and LW-13 was defective to sense white, green and infrared lights. The locomotion of LW-13 was reduced compared with G88 at 2.5, 10, 20, 60, 80, 100, and 200 lx under the green light, but the locomotion of LW-13 female was recovered at 80, 100 and 200 lx. The defective phototaxis to the green light of male LW-13 was not affected by light intensity, while the defective phototaxis to the green light of female LW-13 was recovered at 10, 20, 60, 80, 100, and 200 lx. Conclusion: LW-opsin is involved in light sensing and locomotion of P. xylostella, providing a potential target gene for controlling the pest. © 2021 Society of Chemical Industry.
MeSH term(s)	Animals ; CRISPR-Cas Systems ; Clustered Regularly Interspaced Short Palindromic Repeats ; Female ; Gene Knockout Techniques ; Male ; Moths/genetics ; Opsins/genetics ; Phototaxis
Chemical Substances	Opsins
Language	English
Publishing date	2021-05-12
Publishing country	England
Document type	Journal Article
ZDB-ID	2001705-4
ISSN	1526-4998 ; 1526-498X
ISSN (online)	1526-4998
ISSN	1526-498X
DOI	10.1002/ps.6405
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Enhancing the electroluminescence efficiency of Si NC/SiO2 superlattice-based light-emitting diodes through hydrogen ion beam treatment.

Fu, Sheng-Wen / Chen, Hui-Ju / Wu, Hsuan-Ta / Chen, Shao-Ping / Shih, Chuan-Feng

Nanoscale

2016 Volume 8, Issue 13, Page(s) 7155–7162

Abstract: This paper presents a novel method for enhancing the electroluminescence (EL) efficiency of ten-period silicon-rich oxide (SRO)/SiO2 superlattice-based light-emitting diodes (LEDs). A hydrogen ion beam (HIB) was used to irradiate each SRO layer of the ... ...

Abstract	This paper presents a novel method for enhancing the electroluminescence (EL) efficiency of ten-period silicon-rich oxide (SRO)/SiO2 superlattice-based light-emitting diodes (LEDs). A hydrogen ion beam (HIB) was used to irradiate each SRO layer of the superlattices to increase the interfacial roughness on the nanoscale and the density of the Si nanocrystals (Si NCs). Fowler-Nordheim (F-N) tunneling was the major mechanism for injecting the carriers into the Si NCs. The barrier height of the F-N tunneling was lowered by forming a nano-roughened interface and the nonradiative Pb centers were passivated through the HIB treatment. Additionally, the reflectance of the LEDs was lowered because of the nano-roughened interface. These factors considerably increased the slope efficiency of EL and the maximum output power of the LEDs. The lighting efficiency increased by an order of magnitude, and the turn-on voltage decreased considerably. This study established an efficient approach for obtaining bright Si NC/SiO2 superlattice-based LEDs.
Language	English
Publishing date	2016-04-07
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2515664-0
ISSN	2040-3372 ; 2040-3364
ISSN (online)	2040-3372
ISSN	2040-3364
DOI	10.1039/c5nr08470a
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Article: [Cyanosis secondary to right to left interatrial shunt through an atrial septal defect in a patient with normal pulmonary pressures].

Zhou, Yong / Jiang, Hai-bin / Chen, Shao-ping

Zhonghua xin xue guan bing za zhi

2011 Volume 39, Issue 8, Page(s) 767

MeSH term(s)	Adolescent ; Cyanosis/complications ; Heart Septal Defects, Atrial/complications ; Humans ; Male ; Pulmonary Wedge Pressure
Language	Chinese
Publishing date	2011-08
Publishing country	China
Document type	Case Reports ; Journal Article
ZDB-ID	603425-1
ISSN	0253-3758
ISSN	0253-3758
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Article: [Safety and efficacy of a domestic made small-waist ventricular septal defect occluder for transcatheter closure of ruptured aneurysm of the sinus of Valsalva].

Chen, Shao-ping / Bai, Yuan / Zhao, Xian-xian / Qin, Yong-wen

Zhonghua xin xue guan bing za zhi

2012 Volume 40, Issue 4, Page(s) 298–301

Abstract: Objective: To evaluate the safety and efficacy of transcatheter closure of ruptured aneurysm of the sinus of Valsalva (RAVS) using a domestic made small-waist ventricular septal defect occluder.: Methods: Between September 2005 and December 2010, ... ...

Abstract	Objective: To evaluate the safety and efficacy of transcatheter closure of ruptured aneurysm of the sinus of Valsalva (RAVS) using a domestic made small-waist ventricular septal defect occluder. Methods: Between September 2005 and December 2010, transcatheter closure of RAVS was randomly performed in 7 patients of (43.5 ± 13.0) years old using domestic made small-waist ventricular septal defect occluder in our department. RAVS was diagnosed by color Doppler echocardiography. The size of the small-waist double-disk occlude selected was 4 to 6 mm larger than the narrowest diameter of the opening of aneurysm. After establishment of the arteriovenous wire loop, the device was deployed by retrograde venous approach in all patients. All patients were followed up in terms of rhythm change, residual shunt, shape of occlude, and possible valve regurgitation by echocardiography for 6 to 12 months. Results: All RAVS were confirmed by aortography. Aortography showed rupture of right coronary sinus into the right ventricle in 6 patients and non-coronary sinus ruptured into right ventricle in another patient. The estimated size of the defect was 4 to 7 (5.0 ± 1.3) mm by aortogram. Ventricular septal defect was evidenced in 2 patients. All defects were successfully occluded without complication. Two patients with ventricular septal defect received combined percutaneous ventricular septal defect closure. After transcatheter RAVS occlusion, pulmonary artery systolic pressure decreased from (35.6 ± 12.7) mm Hg (1 mm Hg = 0.133 kPa) to (27.4 ± 6.2) mm Hg (P < 0.05), and mean pulmonary artery pressure decreased from (21.9 ± 8.0) mm Hg to (16.1 ± 5.3) mm Hg (P < 0.05). Cardiac murmur disappeared right after successful occlusion. Echocardiography detected a trace of residual shunt in one patient after occlusion which disappeared the next day. There was no aortic regurgitation, hemolysis and arrhythmia during hospitalization. There was no device embolization, infective endocarditis, right heart failure and death during the 6 to 12 months follow-up. Conclusion: Transcatheter closure of RAVS with the domestic made small-waist ventricular septal defect occluder is safe and effective.
MeSH term(s)	Adult ; Aged ; Aneurysm, Ruptured/surgery ; Cardiac Catheterization/instrumentation ; Cardiac Catheterization/methods ; Female ; Heart Aneurysm ; Humans ; Male ; Middle Aged ; Septal Occluder Device ; Sinus of Valsalva/pathology ; Treatment Outcome ; Young Adult
Language	Chinese
Publishing date	2012-04
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	603425-1
ISSN	0253-3758
ISSN	0253-3758
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