Article ; Online: Ammonia-oxidizing bacteria-driven autotrophic nitrification dominated nitrous oxide production in calcareous soil under long term plastic film mulching
Geoderma. 2023 July, v. 435 p.116523-
2023
Abstract: Fertilized cropland soils are a major source of anthropogenic N₂O emissions in terrestrial ecosystems. However, the main N₂O production pathways and microbes underlying N₂O production in calcareous cropland soil under long-term plastic film mulching and ... ...
| Abstract | Fertilized cropland soils are a major source of anthropogenic N₂O emissions in terrestrial ecosystems. However, the main N₂O production pathways and microbes underlying N₂O production in calcareous cropland soil under long-term plastic film mulching and nitrogen (N) management have not yet been clarified. Here, we used ¹⁵N tracing combined with quantitative polymerase chain reaction to evaluate the relative contributions of different N₂O production pathways (i.e., autotrophic nitrification, heterotrophic nitrification, and denitrification) to N₂O production in a calcareous maize field soil; we also used specific inhibitors (acetylene and 1-octyne) to determine the functional importance of ammonia-oxidizing bacteria (AOB) and archaea (AOA) to N₂O production. Autotrophic nitrification accounted for 47-63% and 76-100% of total N₂O emissions in unfertilized (F₀) and N-fertilized soils, respectively. AOB accounted for 62% and 91-98% of total N₂O production in F₀ and N-fertilized soils, respectively. N₂O production was significantly lower in soils following long-term film mulching (F₂₂₅) compared with the bare land soil (N₂₂₅), and this was due to the reduced abundance and activity of AOB and N₂O derived from autotrophic nitrification. Autotrophic nitrification-derived N₂O emissions were 2.2-fold, 3.6-fold, and 6.3-fold higher in the F₂₂₅ treatment (appropriate N level), the treatment in which the level of N was the same as that applied by local farmers (F₃₈₀), and the manure addition treatment (F₂₂₅₊M) compared with F₀, respectively, indicating that autotrophic nitrification was the main driver of increases in N₂O emissions. These findings provide the first direct evidence showing that AOB-driven autotrophic nitrification is the dominant N₂O production pathway in the plastic film mulched calcareous soils under different agricultural management practices. Our results also show that autotrophic nitrification is depressed by long-term mulching and promoted by N fertilization. These findings have implications for the development of targeted N₂O mitigation strategies. |
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| Keywords | acetylene ; agricultural management ; calcareous soils ; corn ; cropland ; denitrification ; nitrification ; nitrogen ; nitrous oxide production ; plastic film ; quantitative polymerase chain reaction ; Mulching ; N2O production pathways ; Ammonia-oxidizing bacteria ; Gene abundance ; Autotrophic nitrification |
| Language | English |
| Dates of publication | 2023-07 |
| Publishing place | Elsevier B.V. |
| Document type | Article ; Online |
| Note | Use and reproduction |
| ZDB-ID | 281080-3 |
| ISSN | 1872-6259 ; 0016-7061 |
| ISSN (online) | 1872-6259 |
| ISSN | 0016-7061 |
| DOI | 10.1016/j.geoderma.2023.116523 |
| Database | NAL-Catalogue (AGRICOLA) |
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