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  1. Book ; Online ; Thesis: Exposure to Extremely Low-Frequency Pulsed Electromagnetic Fields Promotes Fracture Healing through Alleviating the Damage Caused by Cigarette Smoke and Modulating the Osteoimmune Microenvironment

    Chen, Yangmengfan [Verfasser] / Sabrina, Ehnert [Akademischer Betreuer]

    2023  

    Author's details Yangmengfan Chen ; Betreuer: Ehnert Sabrina
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language English
    Publisher Universitätsbibliothek Tübingen
    Publishing place Tübingen
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  2. Article: Primary cilia: The central role in the electromagnetic field induced bone healing.

    Chen, Yangmengfan / Lu, Chao / Shang, Xifu / Wu, Kerong / Chen, Kun

    Frontiers in pharmacology

    2022  Volume 13, Page(s) 1062119

    Abstract: Primary cilia have emerged as the cellular "antenna" that can receive and transduce extracellular chemical/physical signals, thus playing an important role in regulating cellular activities. Although the electromagnetic field (EMF) is an effective ... ...

    Abstract Primary cilia have emerged as the cellular "antenna" that can receive and transduce extracellular chemical/physical signals, thus playing an important role in regulating cellular activities. Although the electromagnetic field (EMF) is an effective treatment for bone fractures since 1978, however, the detailed mechanisms leading to such positive effects are still unclear. Primary cilia may play a central role in receiving EMF signals, translating physical signals into biochemical information, and initiating various signalingsignaling pathways to transduce signals into the nucleus. In this review, we elucidated the process of bone healing, the structure, and function of primary cilia, as well as the application and mechanism of EMF in treating fracture healing. To comprehensively understand the process of bone healing, we used bioinformatics to analyze the molecular change and associated the results with other studies. Moreover, this review summarizedsummarized some limitations in EMFs-related research and provides an outlook for ongoing studies. In conclusion, this review illustrated the primary cilia and related molecular mechanisms in the EMF-induced bone healing process, and it may shed light on future research.
    Language English
    Publishing date 2022-11-29
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2022.1062119
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Conference proceedings: Intermittent exposure to a 16 Hz extremely low-frequency pulsed electromagnetic field promotes osteogenesis in vitro by piezo1-mediated Ca2+ influx

    Ehnert, Sabrina / Chen, Yangmengfan / Ronniger, Michael / Falldorf, Karsten / Nüssler, Andreas

    2023  , Page(s) AB60–2946

    Event/congress Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2023); Berlin; ; Berufsverband für Orthopädie und Unfallchirurgie; 2023
    Keywords Medizin, Gesundheit ; EMF ; Piezo1 ; osteoprogenitor cells ; calcium influx ; osteogenesis
    Publishing date 2023-10-23
    Publisher German Medical Science GMS Publishing House; Düsseldorf
    Document type Conference proceedings
    DOI 10.3205/23dkou295
    Database German Medical Science

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  4. Article: Intermittent Exposure to a 16 Hz Extremely Low Frequency Pulsed Electromagnetic Field Promotes Osteogenesis In Vitro through Activating Piezo 1-Induced Ca

    Chen, Yangmengfan / Braun, Benedikt J / Menger, Maximilian M / Ronniger, Michael / Falldorf, Karsten / Histing, Tina / Nussler, Andreas K / Ehnert, Sabrina

    Journal of functional biomaterials

    2023  Volume 14, Issue 3

    Abstract: Exposure to extremely low frequency pulsed electromagnetic fields (ELF-PEMF) is supposed to simulate local EMF generated during mechanical stimulation of bone and may therefore be used to improve bone regeneration. This study aimed at optimizing the ... ...

    Abstract Exposure to extremely low frequency pulsed electromagnetic fields (ELF-PEMF) is supposed to simulate local EMF generated during mechanical stimulation of bone and may therefore be used to improve bone regeneration. This study aimed at optimizing the exposure strategy and investigating the underlying mechanisms of a 16 Hz ELF-PEMF, previously reported to boost osteoblast function. Comparing influences of daily continuous (30 min every 24 h) and intermittent (10 min every 8 h) exposure to the 16 Hz ELF-PEMF on osteoprogenitor cells revealed that the intermittent exposure strategy enhanced the 16 Hz ELF-PEMF effects regarding cell numbers and osteogenic function. Gene expression of
    Language English
    Publishing date 2023-03-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2648525-4
    ISSN 2079-4983
    ISSN 2079-4983
    DOI 10.3390/jfb14030165
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: The application of exosomes in the early diagnosis and treatment of osteoarthritis.

    Chen, Anjing / Chen, Yangmengfan / Rong, Xiao / You, Xuanhe / Wu, Diwei / Zhou, Xinran / Zeng, Weinan / Zhou, Zongke

    Frontiers in pharmacology

    2023  Volume 14, Page(s) 1154135

    Abstract: With the increase in human lifespan and the aggravation of global aging, the incidence of osteoarthritis (OA) is increasing annually. To better manage and control the progression of OA, prompt diagnosis and treatment for early-stage OA are important. ... ...

    Abstract With the increase in human lifespan and the aggravation of global aging, the incidence of osteoarthritis (OA) is increasing annually. To better manage and control the progression of OA, prompt diagnosis and treatment for early-stage OA are important. However, a sensitive diagnostic modality and therapy for early OA have not been well developed. The exosome is a class of extracellular vesicles containing bioactive substances, that can be delivered directly from original cells to neighboring cells to modulate cellular activities through intercellular communication. In recent years, exosomes have been considered important in the early diagnosis and treatment of OA. Synovial fluid exosome and its encapsulated substances, e.g., microRNA, lncRNA, and proteins, can not only distinguish OA stages but also prevent the progression of OA by directly targeting cartilage or indirectly modulating the immune microenvironment in the joints. In this mini-review, we include recent studies on the diagnostic and therapeutic modalities of exosomes and hope to provide a new direction for the early diagnosis and treatment of OA disease in the future.
    Language English
    Publishing date 2023-04-28
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2023.1154135
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: NET Formation Was Reduced via Exposure to Extremely Low-Frequency Pulsed Electromagnetic Fields.

    Linnemann, Caren / Sahin, Filiz / Chen, Yangmengfan / Falldorf, Karsten / Ronniger, Michael / Histing, Tina / Nussler, Andreas K / Ehnert, Sabrina

    International journal of molecular sciences

    2023  Volume 24, Issue 19

    Abstract: Fracture-healing is a highly complex and timely orchestrated process. Non-healing fractures are still a major clinical problem and treatment remains difficult. A 16 Hz extremely low-frequency pulsed electromagnetic field (ELF-PEMF) was identified as non- ... ...

    Abstract Fracture-healing is a highly complex and timely orchestrated process. Non-healing fractures are still a major clinical problem and treatment remains difficult. A 16 Hz extremely low-frequency pulsed electromagnetic field (ELF-PEMF) was identified as non-invasive adjunct therapy supporting bone-healing by inducing reactive oxygen species (ROS) and Ca
    MeSH term(s) Humans ; Reactive Oxygen Species ; Hydrogen Peroxide ; Electromagnetic Fields/adverse effects ; Fracture Healing
    Chemical Substances Reactive Oxygen Species ; Hydrogen Peroxide (BBX060AN9V)
    Language English
    Publishing date 2023-09-27
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms241914629
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Decoding the "Fingerprint" of Implant Materials: Insights into the Foreign Body Reaction.

    Chen, Yangmengfan / Luo, Zeyu / Meng, Weikun / Liu, Kai / Chen, Qiqing / Cai, Yongrui / Ding, Zichuan / Huang, Chao / Zhou, Zongke / Jiang, Meng / Zhou, Liqiang

    Small (Weinheim an der Bergstrasse, Germany)

    2024  , Page(s) e2310325

    Abstract: Foreign body reaction (FBR) is a prevalent yet often overlooked pathological phenomenon, particularly within the field of biomedical implantation. The presence of FBR poses a heavy burden on both the medical and socioeconomic systems. This review seeks ... ...

    Abstract Foreign body reaction (FBR) is a prevalent yet often overlooked pathological phenomenon, particularly within the field of biomedical implantation. The presence of FBR poses a heavy burden on both the medical and socioeconomic systems. This review seeks to elucidate the protein "fingerprint" of implant materials, which is generated by the physiochemical properties of the implant materials themselves. In this review, the activity of macrophages, the formation of foreign body giant cells (FBGCs), and the development of fibrosis capsules in the context of FBR are introduced. Additionally, the relationship between various implant materials and FBR is elucidated in detail, as is an overview of the existing approaches and technologies employed to alleviate FBR. Finally, the significance of implant components (metallic materials and non-metallic materials), surface CHEMISTRY (charge and wettability), and physical characteristics (topography, roughness, and stiffness) in establishing the protein "fingerprint" of implant materials is also well documented. In conclusion, this review aims to emphasize the importance of FBR on implant materials and provides the current perspectives and approaches in developing implant materials with anti-FBR properties.
    Language English
    Publishing date 2024-01-08
    Publishing country Germany
    Document type Journal Article ; Review
    ZDB-ID 2168935-0
    ISSN 1613-6829 ; 1613-6810
    ISSN (online) 1613-6829
    ISSN 1613-6810
    DOI 10.1002/smll.202310325
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  8. Article ; Online: The role of Ca

    Ren, Ranyue / Guo, Jiachao / Chen, Yangmengfan / Zhang, Yayun / Chen, Liangxi / Xiong, Wei

    Cell proliferation

    2021  Volume 54, Issue 11, Page(s) e13122

    Abstract: The bone remodelling process is closely related to bone health. Osteoblasts and osteoclasts participate in the bone remodelling process under the regulation of various factors inside and outside. Excessive activation of osteoclasts or lack of function of ...

    Abstract The bone remodelling process is closely related to bone health. Osteoblasts and osteoclasts participate in the bone remodelling process under the regulation of various factors inside and outside. Excessive activation of osteoclasts or lack of function of osteoblasts will cause occurrence and development of multiple bone-related diseases. Ca
    MeSH term(s) Animals ; Calcineurin/metabolism ; Calcium/metabolism ; Cell Differentiation/physiology ; Humans ; NFATC Transcription Factors/metabolism ; Osteoblasts/cytology ; Osteoclasts/metabolism
    Chemical Substances NFATC Transcription Factors ; Calcineurin (EC 3.1.3.16) ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2021-09-15
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 1064202-x
    ISSN 1365-2184 ; 0008-8730 ; 0960-7722
    ISSN (online) 1365-2184
    ISSN 0008-8730 ; 0960-7722
    DOI 10.1111/cpr.13122
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 532: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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  9. Article: Microcurrent Reverses Cigarette Smoke-Induced Angiogenesis Impairment in Human Keratinocytes In Vitro.

    Lu, Chao / Prahm, Cosima / Chen, Yangmengfan / Ehnert, Sabrina / Rinderknecht, Helen / McCaig, Colin D / Nussler, Andreas K / Kolbenschlag, Jonas

    Bioengineering (Basel, Switzerland)

    2022  Volume 9, Issue 9

    Abstract: Cigarette smoking (CS) leads to several adverse health effects, including diseases, disabilities, and even death. Post-operative and trauma patients who smoke have an increased risk for complications, such as delayed bone or wound healing. In clinical ... ...

    Abstract Cigarette smoking (CS) leads to several adverse health effects, including diseases, disabilities, and even death. Post-operative and trauma patients who smoke have an increased risk for complications, such as delayed bone or wound healing. In clinical trials, microcurrent (MC) has been shown to be a safe, non-invasive, and effective way to accelerate wound healing. Our study aimed to investigate if MC with the strength of 100 μA may be beneficial in treating CS-related healing impairment, especially in regard to angiogenesis. In this study, we investigated the effect of human keratinocyte cells (HaCaT) on angiogenesis after 72 h of cigarette smoke extract (CSE) exposure in the presence or absence of 100 μA MC. Cell viability and proliferation were evaluated by resazurin conversion, Sulforhodamine B, and Calcein-AM/Hoechst 33342 staining; the pro-angiogenic potential of HaCaT cells was evaluated by tube formation assay and angiogenesis array assay; signaling pathway alterations were investigated using Western blot. Constant exposure for 72 h to a 100 μA MC enhanced the angiogenic ability of HaCaT cells, which was mediated through the PI3K-Akt signaling pathway. In conclusion, the current data indicate that 100 μA MC may support wound healing in smoking patients by enhancing angiogenesis.
    Language English
    Publishing date 2022-09-06
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2746191-9
    ISSN 2306-5354
    ISSN 2306-5354
    DOI 10.3390/bioengineering9090445
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: A Novel Method to Achieve Precision and Reproducibility in Exposure Parameters for Low-Frequency Pulsed Magnetic Fields in Human Cell Cultures.

    Ronniger, Michael / Aguida, Blanche / Stacke, Christina / Chen, Yangmengfan / Ehnert, Sabrina / Erdmann, Niklas / Eschenburg, Georg / Falldorf, Karsten / Pooam, Marootpong / Wing, Anthony / Ahmad, Margaret

    Bioengineering (Basel, Switzerland)

    2022  Volume 9, Issue 10

    Abstract: The effects of extremely low-frequency electromagnetic field (ELF-MF) exposure on living systems have been widely studied at the fundamental level and also claimed as beneficial for the treatment of diseases for over 50 years. However, the underlying ... ...

    Abstract The effects of extremely low-frequency electromagnetic field (ELF-MF) exposure on living systems have been widely studied at the fundamental level and also claimed as beneficial for the treatment of diseases for over 50 years. However, the underlying mechanisms and cellular targets of ELF-MF exposure remain poorly understood and the field has been plagued with controversy stemming from an endemic lack of reproducibility of published findings. To address this problem, we here demonstrate a technically simple and reproducible EMF exposure protocol to achieve a standardized experimental approach which can be readily adopted in any lab. As an assay system, we chose a commercially available inflammatory model human cell line; its response to magnetic fields involves changes in gene expression which can be monitored by a simple colorimetric reporter gene assay. The cells were seeded and cultured in microplates and inserted into a custom-built, semi-automated incubation and exposure system which accurately controls the incubation (temperature, humidity, CO2) and magnetic-field exposure conditions. A specific alternating magnetic field (<1.0% spatial variance) including far-field reduction provided defined exposure conditions at the position of each well of the microplate. To avoid artifacts, all environmental and magnetic-field exposure parameters were logged in real time throughout the duration of the experiment. Under these extensively controlled conditions, the effect of the magnetic field on the cell cultures as assayed by the standardized operating procedure was highly reproducible between experiments. As we could fully define the characteristics (frequency, intensity, duration) of the pulsed magnetic field signals at the position of the sample well, we were, for the first time, able to accurately determine the effect of changing single ELF-MF parameters such as signal shape, frequency, intensity and duty cycle on the biological response. One signal in particular (10 Hz, 50% duty cycle, rectangular, bipolar, 39.6μT) provided a significant reduction in cytokine reporter gene expression by 37% in our model cell culture line. In sum, the accuracy, environmental control and data-logging capacity of the semi-automated exposure system should greatly facilitate research into fundamental cellular response mechanisms and achieve the consistency necessary to bring ELF-MF/PEMF research results into the scientific mainstream.
    Language English
    Publishing date 2022-10-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2746191-9
    ISSN 2306-5354
    ISSN 2306-5354
    DOI 10.3390/bioengineering9100595
    Database MEDical Literature Analysis and Retrieval System OnLINE

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