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  1. Book ; Online: CRISPR Technology

    Chen, Yuan-Chuan

    Recent Advances

    2023  

    Keywords Biotechnology ; Biochemical engineering
    Language English
    Size 1 electronic resource (158 pages)
    Publisher IntechOpen
    Document type Book ; Online
    Note English
    HBZ-ID HT030378836
    ISBN 9781803568171 ; 1803568178
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Book ; Online: Gene Expression and Phenotypic Traits

    Chen, Yuan-Chuan / Chen, Shiu-Jau

    2020  

    Keywords Medical genetics
    Size 1 electronic resource (210 pages)
    Publisher IntechOpen
    Document type Book ; Online
    Note English ; Open Access
    HBZ-ID HT021045203
    ISBN 9781838803186 ; 1838803181
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  3. Book ; Online: Gene Editing : Technologies and Applications

    Chen, Yuan-Chuan / Chen, Shiu-Jau

    2019  

    Keywords Medical genetics
    Size 1 electronic resource (108 pages)
    Publisher IntechOpen
    Document type Book ; Online
    Note English ; Open Access
    HBZ-ID HT021049036
    ISBN 9781838806897 ; 183880689X
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  4. Book ; Online: Biopharmaceuticals

    Yeh, Ming-Kung / Chen, Yuan-Chuan

    2018  

    Keywords Pharmacology ; immunotherapy, vaccine, mass spectrometry, regenerative medicine, quality control, monoclonal antibody
    Language English
    Size 1 electronic resource (138 pages)
    Publisher IntechOpen
    Document type Book ; Online
    Note English
    HBZ-ID HT030645409
    ISBN 9781838817084 ; 1838817085
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  5. Article ; Online: CRISPR based genome editing and removal of human viruses.

    Chen, Yuan-Chuan

    Progress in molecular biology and translational science

    2021  Volume 179, Page(s) 93–116

    Abstract: The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated proteins 9 (Cas9), a gene-editing technology, has been extensively applied as a tool for genetic engineering in basic research. Efficient genome engineering has been ... ...

    Abstract The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated proteins 9 (Cas9), a gene-editing technology, has been extensively applied as a tool for genetic engineering in basic research. Efficient genome engineering has been performed in viruses, human cells, bacteria, fungi, plants and animals, etc. Currently, it has been employed to edit human viruses for studying viral molecular biology, pathogenesis and oncogenesis, and facilitate the development of antiviral agents and vaccine. The virus is ubiquitous worldwide and elicits global health problems, many human diseases are associated with virus infections. Although traditional drugs can be used to treat or prevent productive viral infections, their efficacy is limited because of toxicity, side effects and other problems. Additionally, no current drugs are approved to be indicated for latent infections. Therefore, the next highlight is to develop antiviral approaches to against both productive and latent infections. Fortunately, CRISPR has been successfully applied in the removal of human viruses ex vivo and/or in vivo, and has the potential to be used to manufacture antiviral agents for clinical application. CRISPR/Cas9 is promising in applications, even though some technical challenges, safety concerns, ethic concerns need to be improved. In this article, the discovery and application of genome editing and removal of human viruses based on CRISPR are explored. Additionally, we evaluate the prospects and limitations of this novel antiviral strategies.
    MeSH term(s) Animals ; CRISPR-Cas Systems/genetics ; Gene Editing ; Genome ; Humans ; Virus Diseases ; Viruses/genetics
    Language English
    Publishing date 2021-01-27
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2471995-X
    ISSN 1878-0814 ; 0079-6603 ; 1877-1173
    ISSN (online) 1878-0814
    ISSN 0079-6603 ; 1877-1173
    DOI 10.1016/bs.pmbts.2020.12.014
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    In stock of ZB MED Cologne/Königswinter

    Uc I Zs 357: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  6. Article: Exploration of Correlations between COVID-19 Vaccination Choice and Public Mental Health Using Google Trend Search.

    Wang, Shao-Cheng / Chen, Yuan-Chuan

    Vaccines

    2022  Volume 10, Issue 12

    Abstract: Since the global COVID-19 pandemic has great impact on human health and life style, the vaccination is the most effective method for disease control and prevention. However, not all people are willing to be vaccinated because some critical factors affect ...

    Abstract Since the global COVID-19 pandemic has great impact on human health and life style, the vaccination is the most effective method for disease control and prevention. However, not all people are willing to be vaccinated because some critical factors affect vaccination aspiration and vaccine choice of the public population. Among these factors, public mental health belongs to a political issue. In this study, Google Trend Search was used to explore the correlation between COVID-19 vaccination choice and public mental health during the period from August/2020 to December/2021. The results suggested that the main public concerns of COVID-19-related mental illnesses are positively correlated with the new cases amount but are negatively correlated with total cases and vaccinated cases amount. Moreover, the results support that the public population took more interest in the Pfizer/BNT COVID vaccine and Moderna COVID vaccine than the AstraZeneca COVID vaccine. Our study shows that investigations of the public mental health should be set up and conducted widely. A complete vaccination program combined with a policy for the improvement of public mental health are very effective for the control and prevention of COVID-19.
    Language English
    Publishing date 2022-12-17
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines10122173
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: External Guide Sequence Effectively Suppresses the Gene Expression and Replication of Herpes Simplex Virus 2.

    Yan, Bin / Liu, Yujun / Chen, Yuan-Chuan / Liu, Fenyong

    Molecules (Basel, Switzerland)

    2024  Volume 29, Issue 9

    Abstract: Ribonuclease P (RNase P) complexed with an external guide sequence (EGS) represents a promising nucleic acid-based gene targeting approach for gene expression knock-down and modulation. The RNase P-EGS strategy is unique as an EGS can be designed to ... ...

    Abstract Ribonuclease P (RNase P) complexed with an external guide sequence (EGS) represents a promising nucleic acid-based gene targeting approach for gene expression knock-down and modulation. The RNase P-EGS strategy is unique as an EGS can be designed to basepair any mRNA sequence and recruit intracellular RNase P for hydrolysis of the target mRNA. In this study, we provide the first direct evidence that the RNase P-based approach effectively blocks the gene expression and replication of herpes simplex virus 2 (HSV-2), the causative agent of genital herpes. We constructed EGSs to target the mRNA encoding HSV-2 single-stranded DNA binding protein ICP8, which is essential for viral DNA genome replication and growth. In HSV-2 infected cells expressing a functional EGS, ICP8 levels were reduced by 85%, and viral growth decreased by 3000 folds. On the contrary, ICP8 expression and viral growth exhibited no substantial differences between cells expressing no EGS and those expressing a disabled EGS with mutations precluding RNase P recognition. The anti-ICP8 EGS is specific in targeting ICP8 because it only affects ICP8 expression but does not affect the expression of the other viral immediate-early and early genes examined. This study shows the effective and specific anti-HSV-2 activity of the RNase P-EGS approach and demonstrates the potential of EGS RNAs for anti-HSV-2 applications.
    MeSH term(s) Herpesvirus 2, Human/genetics ; Herpesvirus 2, Human/physiology ; Virus Replication ; Gene Expression Regulation, Viral ; Humans ; Ribonuclease P/metabolism ; Ribonuclease P/genetics ; Animals ; Viral Proteins/genetics ; Viral Proteins/metabolism ; Chlorocebus aethiops ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Vero Cells ; Immediate-Early Proteins/genetics ; Immediate-Early Proteins/metabolism ; DNA-Binding Proteins
    Chemical Substances Ribonuclease P (EC 3.1.26.5) ; Viral Proteins ; ICP8 protein, Simplexvirus ; RNA, Messenger ; Immediate-Early Proteins ; DNA-Binding Proteins
    Language English
    Publishing date 2024-04-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules29092052
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.MO 81: Show issues

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  8. Article: Challenges and Recent Advancements in COVID-19 Vaccines.

    Wang, Shao-Cheng / Rai, Chung-I / Chen, Yuan-Chuan

    Microorganisms

    2023  Volume 11, Issue 3

    Abstract: Vaccination is the most effective method for the prevention of COVID-19 caused by SARS-CoV-2, which is still a global epidemic. However, the evolution of SARS-CoV-2 is so rapid that various variants, including the Alpha, Beta, Gamma, Delta, and Omicron ... ...

    Abstract Vaccination is the most effective method for the prevention of COVID-19 caused by SARS-CoV-2, which is still a global epidemic. However, the evolution of SARS-CoV-2 is so rapid that various variants, including the Alpha, Beta, Gamma, Delta, and Omicron variants, have emerged, lowering the protection rate of vaccines and even resulting in breakthrough infections. Additionally, some rare but severe adverse reactions induced by COVID-19 vaccines may raise safety concerns and hinder vaccine promotion; however, clinical studies have shown that the benefits of vaccination outweigh the risks caused by adverse reactions. Current vaccines approved with emergency use authorization (EUA) were originally adaptive for adults only, and infants, children, and adolescents are not included. New-generation vaccines are needed to overcome the challenges of limited adaptive age population, breakthrough infection (mainly due to virus variant emergencies), and critical adverse reactions. Fortunately, some advances in COVID-19 vaccines have been obtained regarding enlarged adaptive populations for clinical applications, such as the Pfizer/BioNTech vaccine and the Moderna vaccine. In this article, we provide a review on the challenges and recent advancements in COVID-19 vaccines. The development of next-generation COVID-19 vaccines should lay emphasis on the expansion of adaptive age populations in all individuals, the induction of immune responses to viral variants, the avoidance or alleviation of rare but potentially critical adverse reactions, and the discovery of subunit vaccines with adjuvants encapsulated in nanoparticles.
    Language English
    Publishing date 2023-03-18
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms11030787
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: A RNase P Ribozyme Inhibits Gene Expression and Replication of Hepatitis B Virus in Cultured Cells.

    Yan, Bin / Liu, Yujun / Chen, Yuan-Chuan / Liu, Fenyong

    Microorganisms

    2023  Volume 11, Issue 3

    Abstract: Hepatitis B virus (HBV), an international public health concern, is a leading viral cause of liver disease, such as hepatocellular carcinoma. Sequence-specific ribozymes derived from ribonuclease P (RNase P) catalytic RNA are being explored for gene ... ...

    Abstract Hepatitis B virus (HBV), an international public health concern, is a leading viral cause of liver disease, such as hepatocellular carcinoma. Sequence-specific ribozymes derived from ribonuclease P (RNase P) catalytic RNA are being explored for gene targeting applications. In this study, we engineered an active RNase P ribozyme, M1-S-A, targeting the overlapping region of HBV S mRNA, pre-S/L mRNA, and pregenomic RNA (pgRNA), all deemed essential for viral infection. Ribozyme M1-S-A cleaved the S mRNA sequence efficiently in vitro. We studied the effect of RNase P ribozyme on HBV gene expression and replication using the human hepatocyte HepG2.2.15 culture model that harbors an HBV genome and supports HBV replication. In these cultured cells, the expression of M1-S-A resulted in a reduction of more than 80% in both HBV RNA and protein levels and an inhibition of about 300-fold in the capsid-associated HBV DNA levels when compared to the cells that did not express any ribozymes. In control experiments, cells expressing an inactive control ribozyme displayed little impact on HBV RNA and protein levels, and on capsid-associated viral DNA levels. Our study signifies that RNase P ribozyme can suppress HBV gene expression and replication, implying the promise of RNase P ribozymes for anti-HBV therapy.
    Language English
    Publishing date 2023-03-03
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms11030654
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Suppressing Kaposi's Sarcoma-Associated Herpesvirus Lytic Gene Expression and Replication by RNase P Ribozyme.

    Liu, Yujun / Chen, Yuan-Chuan / Yan, Bin / Liu, Fenyong

    Molecules (Basel, Switzerland)

    2023  Volume 28, Issue 8

    Abstract: Kaposi's sarcoma, an AIDS-defining illness, is caused by Kaposi's sarcoma-associated herpesvirus (KSHV), an oncogenic virus. In this study, we engineered ribozymes derived from ribonuclease P (RNase P) catalytic RNA with targeting against the mRNA ... ...

    Abstract Kaposi's sarcoma, an AIDS-defining illness, is caused by Kaposi's sarcoma-associated herpesvirus (KSHV), an oncogenic virus. In this study, we engineered ribozymes derived from ribonuclease P (RNase P) catalytic RNA with targeting against the mRNA encoding KSHV immediate early replication and transcription activator (RTA), which is vital for KSHV gene expression. The functional ribozyme F-RTA efficiently sliced the RTA mRNA sequence in vitro. In cells, KSHV production was suppressed with ribozyme F-RTA expression by 250-fold, and RTA expression was suppressed by 92-94%. In contrast, expression of control ribozymes hardly affected RTA expression or viral production. Further studies revealed both overall KSHV early and late gene expression and viral growth decreased because of F-RTA-facilitated suppression of RTA expression. Our results indicate the first instance of RNase P ribozymes having potential for use in anti-KSHV therapy.
    MeSH term(s) Herpesvirus 8, Human/genetics ; Herpesvirus 8, Human/metabolism ; RNA, Catalytic/genetics ; RNA, Catalytic/metabolism ; Ribonuclease P/genetics ; Ribonuclease P/metabolism ; Immediate-Early Proteins/metabolism ; Virus Replication/genetics ; Virus Latency ; Trans-Activators/genetics ; RNA, Messenger/genetics ; Gene Expression ; Gene Expression Regulation, Viral
    Chemical Substances RNA, Catalytic ; Ribonuclease P (EC 3.1.26.5) ; Immediate-Early Proteins ; Trans-Activators ; RNA, Messenger
    Language English
    Publishing date 2023-04-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules28083619
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.MO 81: Show issues

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