Artikel ; Online: Cyclic-di-GMP binds to histidine kinase RavS to control RavS-RavR phosphotransfer and regulates the bacterial lifestyle transition between virulence and swimming.
2019 Band 15, Heft 8, Seite(n) e1007952
Abstract: The two-component signalling system (TCS) comprising a histidine kinase (HK) and a response regulator (RR) is the predominant bacterial sense-and-response machinery. Because bacterial cells usually encode a number of TCSs to adapt to various ecological ... ...
| Abstract | The two-component signalling system (TCS) comprising a histidine kinase (HK) and a response regulator (RR) is the predominant bacterial sense-and-response machinery. Because bacterial cells usually encode a number of TCSs to adapt to various ecological niches, the specificity of a TCS is in the centre of regulation. Specificity of TCS is defined by the capability and velocity of phosphoryl transfer between a cognate HK and a RR. Here, we provide genetic, enzymology and structural data demonstrating that the second messenger cyclic-di-GMP physically and specifically binds to RavS, a HK of the phytopathogenic, gram-negative bacterium Xanthomonas campestris pv. campestris. The [c-di-GMP]-RavS interaction substantially promotes specificity between RavS and RavR, a GGDEF-EAL domain-containing RR, by reinforcing the kinetic preference of RavS to phosphorylate RavR. [c-di-GMP]-RavS binding effectively decreases the phosphorylation level of RavS and negatively regulates bacterial swimming. Intriguingly, the EAL domain of RavR counteracts the above regulation by degrading c-di-GMP and then increasing the level of phosphorylated RavS. Therefore, RavR acts as a bifunctional phosphate sink that finely controls the level of phosphorylated RavS. These biochemical processes interactively modulate the phosphoryl flux between RavS-RavR and bacterial lifestyle transition. Our results revealed that c-di-GMP acts as an allosteric effector to dynamically modulate specificity between HK and RR. |
|---|---|
| Mesh-Begriff(e) | Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Biofilms/growth & development ; Cyclic GMP/analogs & derivatives ; Cyclic GMP/metabolism ; Flagella/physiology ; Gene Expression Regulation, Bacterial ; Histidine Kinase/genetics ; Histidine Kinase/metabolism ; Phosphorylation ; Signal Transduction ; Virulence/physiology ; Xanthomonas campestris/genetics ; Xanthomonas campestris/growth & development ; Xanthomonas campestris/metabolism |
| Chemische Substanzen | Bacterial Proteins ; bis(3',5')-cyclic diguanylic acid (61093-23-0) ; Histidine Kinase (EC 2.7.13.1) ; Cyclic GMP (H2D2X058MU) |
| Sprache | Englisch |
| Erscheinungsdatum | 2019-08-13 |
| Erscheinungsland | United States |
| Dokumenttyp | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2205412-1 |
| ISSN | 1553-7374 ; 1553-7366 |
| ISSN (online) | 1553-7374 |
| ISSN | 1553-7366 |
| DOI | 10.1371/journal.ppat.1007952 |
| Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
Zusatzmaterialien
Kategorien
Über subito bestellen
Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.