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  1. Article: Using a smartphone app in the measurement of posture-related pupil center shift on centration during corneal refractive surgery.

    Cheng, Wenbo / Li, Li / Luo, Gang / Wang, Yan

    Frontiers in cell and developmental biology

    2023  Volume 11, Page(s) 1174122

    Abstract: Purpose: ...

    Abstract Purpose:
    Language English
    Publishing date 2023-04-13
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2737824-X
    ISSN 2296-634X
    ISSN 2296-634X
    DOI 10.3389/fcell.2023.1174122
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Identification of plant microRNAs using convolutional neural network.

    Zhang, Yun / Huang, Jianghua / Xie, Feixiang / Huang, Qian / Jiao, Hongguan / Cheng, Wenbo

    Frontiers in plant science

    2024  Volume 15, Page(s) 1330854

    Abstract: MicroRNAs (miRNAs) are of significance in tuning and buffering gene expression. Despite abundant analysis tools that have been developed in the last two decades, plant miRNA identification from next-generation sequencing (NGS) data remains challenging. ... ...

    Abstract MicroRNAs (miRNAs) are of significance in tuning and buffering gene expression. Despite abundant analysis tools that have been developed in the last two decades, plant miRNA identification from next-generation sequencing (NGS) data remains challenging. Here, we show that we can train a convolutional neural network to accurately identify plant miRNAs from NGS data. Based on our methods, we also present a user-friendly pure Java-based software package called Small RNA-related Intelligent and Convenient Analysis Tools (SRICATs). SRICATs encompasses all the necessary steps for plant miRNA analysis. Our results indicate that SRICATs outperforms currently popular software tools on the test data from five plant species. For non-commercial users, SRICATs is freely available at https://sourceforge.net/projects/sricats.
    Language English
    Publishing date 2024-03-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2024.1330854
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Rapid classification and identification of chemical compositions of Pu-zhi-hui-ling decoction by UHPLC-Q-Orbitrap HRMS.

    Shao, Jia / Zheng, Yanxue / Wang, Yuanyuan / Li, Guohui / Wei, Jinxia / Cheng, Wenbo / Li, Yubo

    Natural product research

    2024  , Page(s) 1–10

    Abstract: Pu-zhi-hui-ling decoction (PZHLD) is a traditional Chinese medicine (TCM) formula for the treatment of Alzheimer's disease (AD), but its chemical composition has not been reported. In this study, we aimed to establish a mass spectrometry (MS) analysis ... ...

    Abstract Pu-zhi-hui-ling decoction (PZHLD) is a traditional Chinese medicine (TCM) formula for the treatment of Alzheimer's disease (AD), but its chemical composition has not been reported. In this study, we aimed to establish a mass spectrometry (MS) analysis method for rapid classification and identification of the chemical constituents in PZHLD. The sample was analysed by ultrahigh-performance liquid chromatography coupled to quadrupole Orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS). The chemical constituents of PZHLD were identified based on accurate MS data, fragmentation characteristics of MS/MS, and reference information described in the literature. A total of 123 chemical constituents were identified. In addition, we summarised the fragmentation pathways of the chemical constituents in PZHLD. Our finding might lay the foundation for the further pharmacodynamic study and clinical application of PZHLD.
    Language English
    Publishing date 2024-01-03
    Publishing country England
    Document type Journal Article
    ZDB-ID 2185747-7
    ISSN 1478-6427 ; 1478-6419
    ISSN (online) 1478-6427
    ISSN 1478-6419
    DOI 10.1080/14786419.2023.2299302
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: A Ribulose-5-phosphate Shunt from the Calvin-Benson Cycle to Methylerythritol Phosphate Pathway for Enhancing Photosynthetic Terpenoid Production.

    Zhou, Jie / Xu, Suxian / Li, Hu / Xi, Huachao / Cheng, Wenbo / Yang, Chen

    ACS synthetic biology

    2024  Volume 13, Issue 3, Page(s) 876–887

    Abstract: Cyanobacteria are attractive hosts for photosynthetic terpenoid production, using ... ...

    Abstract Cyanobacteria are attractive hosts for photosynthetic terpenoid production, using CO
    MeSH term(s) Terpenes/metabolism ; Phosphates ; Carbon Dioxide/metabolism ; Photosynthesis ; Carbon ; Pentanols ; Pentoses
    Chemical Substances Terpenes ; isopentenol (27214-40-0) ; Phosphates ; Carbon Dioxide (142M471B3J) ; ribulose (5556-48-9) ; Carbon (7440-44-0) ; Pentanols ; Pentoses
    Language English
    Publishing date 2024-02-16
    Publishing country United States
    Document type Journal Article
    ISSN 2161-5063
    ISSN (online) 2161-5063
    DOI 10.1021/acssynbio.3c00675
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Rapid identification of chemical components in vitro and in vivo of Menispermi Rhizoma by integrating UPLC‐Q‐TOF‐MS with data post‐processing strategy

    Wei, Jinxia / Wang, Yuanyuan / Zhang, Yue / Zheng, Yanxue / Shao, Jia / Cheng, Wenbo / Li, Yubo

    Phytochemical Analysis. 2023 Apr., v. 34, no. 3 p.347-362

    2023  

    Abstract: INTRODUCTION: Menispermi Rhizoma (MR), the dried rhizome of Menispermum dauricum DC. (Menispermaceae), has been used to treat sore throat, enteritis, dysentery, and rheumatic arthralgia. Despite extensive research on its pharmacological effects, the ... ...

    Abstract INTRODUCTION: Menispermi Rhizoma (MR), the dried rhizome of Menispermum dauricum DC. (Menispermaceae), has been used to treat sore throat, enteritis, dysentery, and rheumatic arthralgia. Despite extensive research on its pharmacological effects, the chemical components in vitro and in vivo have not been thoroughly studied. OBJECTIVE: To establish an efficient method for rapid classification and identification of alkaloids in MR and its preparations, as well as metabolites in vivo after oral administration of MR. METHODS: Rapid identification of alkaloids and absorbed components of MR was performed using ultra‐performance liquid chromatography quadrupole time‐of‐flight mass spectrometry (UPLC‐Q‐TOF‐MS) coupled with UNIFI software. Moreover, the characteristic fragmentations and neutral losses of different types of alkaloids in MR were summarised to realise the rapid classification of alkaloids. RESULTS: A total of 55 components were unambiguously or tentatively identified in MR. Among them, 37 and 31 components were found in MR capsules and tablets, respectively. Meanwhile, 109 compounds were tentatively identified in rat plasma, urine and faeces, including 55 prototypes and 54 metabolites. Hydrogenation, hydroxylation, methylation, glucuronic acid and sulphate conjugations were the dominating metabolic fates of alkaloids. CONCLUSION: The data post‐processing strategy established could greatly enhance the structural identification efficiency. The results obtained might lay the foundation for further interpretation of clinical effects, mechanism of action and quality control of MR.
    Keywords Menispermum dauricum ; computer software ; dysentery ; enteritis ; feces ; glucuronic acid ; hydrogenation ; hydroxylation ; mass spectrometry ; metabolites ; methylation ; oral administration ; pharyngitis ; phytochemicals ; rats ; rhizomes ; sulfates ; ultra-performance liquid chromatography ; urine
    Language English
    Dates of publication 2023-04
    Size p. 347-362.
    Publishing place John Wiley & Sons, Ltd
    Document type Article ; Online
    Note JOURNAL ARTICLE
    ZDB-ID 1073576-8
    ISSN 1099-1565 ; 0958-0344
    ISSN (online) 1099-1565
    ISSN 0958-0344
    DOI 10.1002/pca.3214
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4608: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  6. Article ; Online: Preparation of magnetic amphiphilic resin microspheres via the one-step polymerization method and extraction of four glucocorticoids for HPLC-MS analysis.

    Zhou, Zibo / He, Liang / Wang, Tianyi / Tang, Hongzhen / Qin, Suzi / Nan, Xueyan / Cheng, Wenbo / He, Haibo / Bai, Pengli

    Journal of chromatography. A

    2024  Volume 1720, Page(s) 464785

    Abstract: Amphiphilic materials can be used for sample preparation of chromatography or mass spectrometry. Amphiphilic materials with magnetic properties in combination with magnetic suction devices allow for automated sample preparation. However, conventional ... ...

    Abstract Amphiphilic materials can be used for sample preparation of chromatography or mass spectrometry. Amphiphilic materials with magnetic properties in combination with magnetic suction devices allow for automated sample preparation. However, conventional synthesis methods are cumbersome and not suitable for the mass production of the material. In this study, a micro-suspension polymerization method was developed to synthesize magnetic amphiphilic resin microspheres (MARMs), providing new ideas for the preparation of amphiphilic microspheres. MARMs with particle sizes ranging from 3 to 6 μm were successfully prepared, with BET surface area up to 653.2 m
    MeSH term(s) Glucocorticoids ; Liquid Chromatography-Mass Spectrometry ; Microspheres ; Polymerization ; Magnetic Phenomena ; Solid Phase Extraction/methods ; Chromatography, High Pressure Liquid
    Chemical Substances Glucocorticoids
    Language English
    Publishing date 2024-03-05
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1171488-8
    ISSN 1873-3778 ; 0021-9673
    ISSN (online) 1873-3778
    ISSN 0021-9673
    DOI 10.1016/j.chroma.2024.464785
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 813: Show issues Location:
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  7. Article ; Online: Recent Progress in DNA Hybridization Chain Reaction Strategies for Amplified Biosensing.

    Chai, Hua / Cheng, Wenbo / Jin, Dayong / Miao, Peng

    ACS applied materials & interfaces

    2021  Volume 13, Issue 33, Page(s) 38931–38946

    Abstract: With the continuous development of DNA nanotechnology, various spatial DNA structures and assembly techniques emerge. Hybridization chain reaction (HCR) is a typical example with exciting features and bright prospects in biosensing, which has been ... ...

    Abstract With the continuous development of DNA nanotechnology, various spatial DNA structures and assembly techniques emerge. Hybridization chain reaction (HCR) is a typical example with exciting features and bright prospects in biosensing, which has been intensively investigated in the past decade. In this Spotlight on Applications, we summarize the assembly principles of conventional HCR and some novel forms of linear/nonlinear HCR. With advantages like great assembly kinetics, facile operation, and an enzyme-free and isothermal reaction, these strategies can be integrated with most mainstream reporters (e.g., fluorescence, electrochemistry, and colorimetry) for the ultrasensitive detection of abundant targets. Particularly, we select several representative studies to better illustrate the novel ideas and performances of HCR strategies. Theoretical and practical utilities are confirmed for a range of biosensing applications. In the end, a deep discussion is provided about the challenges and future tasks of this field.
    MeSH term(s) Biosensing Techniques ; Colorimetry ; DNA/chemistry ; Electrochemical Techniques ; Humans ; Nanoparticles/chemistry ; Nucleic Acid Amplification Techniques/methods ; Nucleic Acid Conformation ; Nucleic Acid Hybridization/methods ; Sensitivity and Specificity ; Spectrometry, Fluorescence
    Chemical Substances DNA (9007-49-2)
    Language English
    Publishing date 2021-08-10
    Publishing country United States
    Document type Journal Article ; Review
    ISSN 1944-8252
    ISSN (online) 1944-8252
    DOI 10.1021/acsami.1c09000
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Machine learning-assisted flexible wearable device for tyrosine detection.

    Bao, Qiwen / Li, Gang / Cheng, Wenbo / Yang, Zhengchun / Qu, Zilian / Wei, Jun / Lin, Ling

    RSC advances

    2023  Volume 13, Issue 34, Page(s) 23788–23795

    Abstract: Early diagnosis of pathological markers can significantly shorten the rate of viral transmission, reduce the probability of infection, and improve the cure rate of diseases. Therefore, analytical techniques for identifying pathological markers and ... ...

    Abstract Early diagnosis of pathological markers can significantly shorten the rate of viral transmission, reduce the probability of infection, and improve the cure rate of diseases. Therefore, analytical techniques for identifying pathological markers and environmental toxicants have received considerable attention from researchers worldwide. However, the most popular techniques used in clinical settings involve expensive precision instruments and complex detection processes. Thus, a simpler, more efficient, rapid, and intelligent means of analysis must be urgently developed. Electrochemical biosensors have the advantages of simple processing, low cost, low sample preparation requirements, rapid analysis, easy miniaturization, and integration. Thus, they have become popular in extensive research. Machine learning is widely used in material-assisted synthesis, sensor design, and other fields owing to its powerful data analysis and simulation learning capabilities. In this study, a machine learning-assisted carbon black-graphene oxide conjugate polymer (CB-GO/CP) electrode, in conjunction with a flexible wearable device, is proposed for the smart portable detection of tyrosine (Tyr). Input feature value data are obtained for the artificial neural network (ANN) and support vector machines (SVM) model learning
    Language English
    Publishing date 2023-08-08
    Publishing country England
    Document type Journal Article
    ISSN 2046-2069
    ISSN (online) 2046-2069
    DOI 10.1039/d3ra02900j
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Model-Based Feedback Control for an Automated Micro Liquid Dispensing System Based on Contacting Droplet Generation through Image Sensing.

    Qian, Qing / Xu, Wenchang / Tian, Haoran / Cheng, Wenbo / Zhou, Lianqun / Wang, Jishuai

    Micromachines

    2023  Volume 14, Issue 10

    Abstract: Over the past few decades, micro liquid dispensing technology has been widely used in biology, chemistry, material and environmental sciences due to its efficacy in processing multiple samples. For practical applications, precise and effective droplet ... ...

    Abstract Over the past few decades, micro liquid dispensing technology has been widely used in biology, chemistry, material and environmental sciences due to its efficacy in processing multiple samples. For practical applications, precise and effective droplet generation is very important. Despite numerous droplet generation methods, the implementation of droplet-on-demand still faces challenges concerning system complexity, precision, cost, and robustness. In this work, a novel on-demand contacting droplet generation method incorporated with model-based feedback control with an image processing unit as a sensor was proposed. By studying droplet identification using image processing techniques, the model of droplet formation was simplified. Then model-based feedback control was implemented using volumes of dispensed samples as sensing signals by tuning related parameters adaptively to resist disturbances. The proposed method was integrated and applied to a homebuilt automated micro liquid dispensing system with droplets ranging from 20 nanoliter to 200 nanoliter. The experimental results demonstrated a high degree of accuracy and precision. Additionally, the proposed system's practical utility was evaluated by analyzing mutations in genes associated with sensorineural hearing loss, verifying its effectiveness.
    Language English
    Publishing date 2023-10-18
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2620864-7
    ISSN 2072-666X
    ISSN 2072-666X
    DOI 10.3390/mi14101938
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Rapid identification of chemical components in vitro and in vivo of Menispermi Rhizoma by integrating UPLC-Q-TOF-MS with data post-processing strategy.

    Wei, Jinxia / Wang, Yuanyuan / Zhang, Yue / Zheng, Yanxue / Shao, Jia / Cheng, Wenbo / Li, Yubo

    Phytochemical analysis : PCA

    2023  Volume 34, Issue 3, Page(s) 347–362

    Abstract: Introduction: Menispermi Rhizoma (MR), the dried rhizome of Menispermum dauricum DC. (Menispermaceae), has been used to treat sore throat, enteritis, dysentery, and rheumatic arthralgia. Despite extensive research on its pharmacological effects, the ... ...

    Abstract Introduction: Menispermi Rhizoma (MR), the dried rhizome of Menispermum dauricum DC. (Menispermaceae), has been used to treat sore throat, enteritis, dysentery, and rheumatic arthralgia. Despite extensive research on its pharmacological effects, the chemical components in vitro and in vivo have not been thoroughly studied.
    Objective: To establish an efficient method for rapid classification and identification of alkaloids in MR and its preparations, as well as metabolites in vivo after oral administration of MR.
    Methods: Rapid identification of alkaloids and absorbed components of MR was performed using ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) coupled with UNIFI software. Moreover, the characteristic fragmentations and neutral losses of different types of alkaloids in MR were summarised to realise the rapid classification of alkaloids.
    Results: A total of 55 components were unambiguously or tentatively identified in MR. Among them, 37 and 31 components were found in MR capsules and tablets, respectively. Meanwhile, 109 compounds were tentatively identified in rat plasma, urine and faeces, including 55 prototypes and 54 metabolites. Hydrogenation, hydroxylation, methylation, glucuronic acid and sulphate conjugations were the dominating metabolic fates of alkaloids.
    Conclusion: The data post-processing strategy established could greatly enhance the structural identification efficiency. The results obtained might lay the foundation for further interpretation of clinical effects, mechanism of action and quality control of MR.
    MeSH term(s) Rats ; Animals ; Tandem Mass Spectrometry/methods ; Chromatography, High Pressure Liquid/methods ; Rhizome/chemistry ; Drugs, Chinese Herbal/chemistry ; Alkaloids/analysis
    Chemical Substances Drugs, Chinese Herbal ; Alkaloids
    Language English
    Publishing date 2023-02-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 1073576-8
    ISSN 1099-1565 ; 0958-0344
    ISSN (online) 1099-1565
    ISSN 0958-0344
    DOI 10.1002/pca.3214
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4608: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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