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Article ; Online: Prevalence and Molecular Characterization of Bovine Parainfluenza Virus Type 3 in Cattle Herds in China

Yunxin Ren / Cheng Tang / Hua Yue

Animals, Vol 13, Iss 793, p

2023 Volume 793

Abstract: Bovine parainfluenza virus type 3 (BPIV3) is a common respiratory pathogen that causes respiratory illness in cattle and makes a major contribution to the bovine respiratory disease complex (BRDC); however, data on the prevalence and molecular features ... ...

Abstract	Bovine parainfluenza virus type 3 (BPIV3) is a common respiratory pathogen that causes respiratory illness in cattle and makes a major contribution to the bovine respiratory disease complex (BRDC); however, data on the prevalence and molecular features of BPIV3 are still scarce in China. To investigate the epidemiological characteristics of BPIV3 in China, between September 2020 and June 2022, 776 respiratory samples were received from 58 BRDC-affected farms located in 16 provinces and one municipality. Those were screened for BPIV3 using a reverse transcription insulated isothermal PCR (RT-iiPCR) assay. Meanwhile, the HN gene and complete genome sequence of strains from different provinces were amplified, sequenced, and analyzed. The tests showed that 18.17% (141/776) of samples tested were positive for BPIV3, which originated from 21 farms in 6 provinces. Moreover, 22 complete HN gene sequences and 9 nearly complete genome sequences were obtained from the positive samples. Phylogenetic analysis based on the HN gene and complete genome sequences revealed that the sequences were clustered in one large clade for all Chinese BPIV3 genotype C strains, while overseas strain sequences of BPIV3 genotype C clustered into other clades. Moving beyond the known complete genome sequences of BPIV3 in GenBank, a total of five unique amino acid mutations were found in N protein, F protein, and HN protein in Chinese BPIV3 genotype C strains. Taken together, this study reveals that BPIV3 genotype C strains, the dominant strains in China, have a broad geographical distribution and some unique genetic characteristics. These findings contribute to our understanding of the epidemiological characteristics and genetic evolution of BPIV3 in China.
Keywords	bovine parainfluenza virus type 3 ; China ; prevalence ; molecular characterization ; phylogenetic analysis ; evolution ; Veterinary medicine ; SF600-1100 ; Zoology ; QL1-991
Subject code	572
Language	English
Publishing date	2023-02-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Detection and Genomic Characterization of Bovine Rhinitis Virus in China

Yuxing Zhou / Xi Chen / Cheng Tang / Hua Yue

Animals, Vol 13, Iss 312, p

2023 Volume 312

Abstract: Bovine rhinitis virus (BRV) is an etiological agent of bovine respiratory disease complex (BRDC) and can be divided into two genotypes—bovine rhinitis A virus (BRAV) and bovine rhinitis B virus (BRBV). However, knowledge about the prevalence and ... ...

Abstract	Bovine rhinitis virus (BRV) is an etiological agent of bovine respiratory disease complex (BRDC) and can be divided into two genotypes—bovine rhinitis A virus (BRAV) and bovine rhinitis B virus (BRBV). However, knowledge about the prevalence and molecular information of BRV in China is still limited. In this study, 163 deep nasal swabs collected from bovines with BRDC syndrome on 16 farms across nine provinces of China were tested for BRAV and BRBV by a duplex real-time RT-PCR assay. The results showed that 28.22% (46/163) of the samples were BRV-positive, and the positive rates were 22.09% (36/163) for BRAV and 9.2% (15/163) for BRBV. The co-circulation of both BRV genotypes was observed on two farms. Furthermore, five near-complete BRV genomes, including three BRAVs and two BRBVs, were obtained. The phylogenetic analysis showed that the three obtained BRAVs were phylogenetically independent, while the two BRBVs exhibited significant genetic heterogeneity. Recombination analysis revealed that three BRAVs and one BRBV strain obtained in this study were recombinants. The present study confirmed the presence and prevalence of BRAV in China, and it found that both types of BRV are circulating in beef cattle, which contributes to a better understanding of the prevalence and molecular characteristics of BRV.
Keywords	bovine rhinitis A virus ; bovine rhinitis B virus ; prevalence ; genomes ; recombination ; Veterinary medicine ; SF600-1100 ; Zoology ; QL1-991
Subject code	630
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Characteristics of elderly diabetes patients

Xiaofei Yang / Chongxiang Xue / Keyu Chen / Dongyang Gao / Han Wang / Cheng Tang

Frontiers in Pharmacology, Vol

focus on clinical manifestation, pathogenic mechanism, and the role of traditional Chinese medicine

2024 Volume 14

Abstract: Diabetes mellitus has become a major public health issue globally, putting an enormous burden on global health systems and people. Among all diseased groups, a considerable part of patients are elderly, while their clinical features, pathogenic processes, ...

Abstract	Diabetes mellitus has become a major public health issue globally, putting an enormous burden on global health systems and people. Among all diseased groups, a considerable part of patients are elderly, while their clinical features, pathogenic processes, and medication regimens are different from patients of other ages. Despite the availability of multiple therapies and techniques, there are still numerous elderly diabetes patients suffering from poor blood glucose control, severe complications, and drug adverse effects, which negatively affect the quality of life in their golden years. Traditional Chinese Medicine (TCM) has been widely used in the treatment of diabetes for several decades, and its relevant clinical practice has confirmed that it has a satisfactory effect on alleviating clinical symptoms and mitigating the progression of complications. Chinese herbal medicine and its active components were used widely with obvious clinical advantages by multiple targets and signaling pathways. However, due to the particular features of elderly diabetes, few studies were conducted to explore Traditional Chinese Medicine intervention on elderly diabetic patients. This study reviews the research on clinical features, pathogenic processes, treatment principles, and TCM treatments, hoping to provide fresh perspectives on the prevention and management strategies for elderly diabetes.
Keywords	elderly diabetes ; traditional Chinese medicine ; clinical manifestation ; pathogenic mechanism ; treatment ; Therapeutics. Pharmacology ; RM1-950
Subject code	616
Language	English
Publishing date	2024-01-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Phosphorylation of Kapok Fiber with Phytic Acid for Enhanced Flame Retardancy

Xin-Lin Jiang / Ren-Cheng Tang

International Journal of Molecular Sciences, Vol 23, Iss 14950, p

2022 Volume 14950

Abstract: Kapok fiber (KF), with the characteristics of a natural hollow structure, light weight, and low density, can be used as acoustic and thermal insulation, buoyancy, adsorption, filling, and composite material. The flame-retardant treatment can expand the ... ...

Abstract	Kapok fiber (KF), with the characteristics of a natural hollow structure, light weight, and low density, can be used as acoustic and thermal insulation, buoyancy, adsorption, filling, and composite material. The flame-retardant treatment can expand the functionality and application of KF. In this work, the phosphorylation of KF using phytic acid (PA) in the presence of urea at a high temperature was used to enhance its flame retardancy. The phosphorylation reaction conditions were discussed, and the surface topography, thermal degradation, heat release, and combustion properties of phosphorylated KF were studied. The Fourier transform infrared spectroscopy and 31 P solid-state nuclear magnetic resonance spectroscopy analyses confirmed the grafting of PA on cellulose by the formation of phosphate ester bonds. Due to the covalent binding of PA, phosphorylated KF exhibited good washing durability. The surface topography, Raman spectroscopy, thermogravimetric (TG), and microcalorimetry analyses revealed the excellent charring ability of phosphorylated KF. In the TG test in nitrogen, the char residue increased to 42.6% of phosphorylated KF from 8.3% of raw KF at 700 °C. In the vertical combustion, raw KF sheet was almost completely burned out within 30 s, while phosphorylated KF was very difficult to catch fire. In the microcalorimetry analysis, the heat release capacity and total heat release of phosphorylated KF decreased to 67 J/g∙K and 3.9 kJ/g, respectively from 237 J/g∙K and 18.1 kJ/g of raw KF. This work suggests that phosphorylated KF is an excellent flame-retardant material.
Keywords	kapok fiber ; flame retardant ; phosphorylation ; phytic acid ; thermal stability ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	660
Language	English
Publishing date	2022-11-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: First Detection and Genomic Characterization of Bovine Norovirus from Yak

Yuchen Cui / Xi Chen / Hua Yue / Cheng Tang

Pathogens, Vol 11, Iss 192, p

2022 Volume 192

Abstract: Yak are a unique free-grazing bovine species in high-altitude areas. The objective of this study was to investigate the presence and molecular characteristics of BNoV in yak. A total of 205 diarrheal samples of yak (aged ≤ 3 months) were collected from ... ...

Abstract	Yak are a unique free-grazing bovine species in high-altitude areas. The objective of this study was to investigate the presence and molecular characteristics of BNoV in yak. A total of 205 diarrheal samples of yak (aged ≤ 3 months) were collected from 10 farms in Sichuan Province, China, from May 2018 to October 2020, and four samples were detected as BNoV-positive with RT-PCR. Moreover, a nearly full-length genome of SMU-YAK-J1 containing three complete ORFs was successfully sequenced. Sequence analysis with only nine genome sequences of the GIII genogroup showed that SMU-YAK-J1 was most closely related with GIII.P2 GIII.4, sharing 90.9% gnomic nucleotide identity, but only shared 71.6–85.9% with other genotypes, which confirmed that SMU-YAK-J1 belongs to genotype GIII.P2 GIII.4. However, compared with the sole genome of GIII.4 in GenBank, the BNoV in this study also exhibited many unique amino acid changes among all the three ORFs, which may represent the unique genetic evolution of BNoV in yak. This study first determined the presence of BNoV in yak, contributing to a better understanding of the prevalence and genetic evolution of BNoV.
Keywords	yak ; bovine norovirus ; detection ; GIII.P2_GIII.4 genome ; Medicine ; R
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Adsorption Performance of Acid Dyes on Color-Adsorbing Nonwoven and Its Application in the Washing of Silk Textiles

Yao Ji / Ren-Cheng Tang / Jin-Ping Guan

Journal of Natural Fibers, Vol 19, Iss 16, Pp 14171-

2022 Volume 14182

Abstract: In recent years, the use of color-adsorbing nonwoven as a dye transfer inhibitor to prevent the cross-staining of fabrics in home laundering has received great attention. In this work, a color-adsorbing nonwoven was used to adsorb the dyes desorbed from ... ...

Abstract	In recent years, the use of color-adsorbing nonwoven as a dye transfer inhibitor to prevent the cross-staining of fabrics in home laundering has received great attention. In this work, a color-adsorbing nonwoven was used to adsorb the dyes desorbed from dyed silk textiles and thereby provide an anti-staining effect in the washing. The nonwoven consisted of cationic regenerated cellulose and polyolefin fibers and had an isoelectric point of 7.82. The adsorption properties of acid dyes on the nonwoven and the anti-staining of the nonwoven were studied. The adsorption kinetics and isotherms of acid dyes on the nonwoven followed the pseudo-second-order kinetic and Langmuir adsorption models, respectively. With increasing temperature, the adsorption saturation increased, and the Langmuir affinity constant decreased, indicating an exothermic effect. The adsorption of dyes with high molecular weight showed great temperature dependence. During the washing of dyed silk textiles, the strong adsorption capability of cationic cellulose for desorbed acid dyes contributed to the anti-staining effect of color-adsorbing nonwoven.
Keywords	silk ; cationic cellulose ; color-adsorbing nonwoven ; acid dyes ; adsorption ; anti-staining ; Science ; Q ; Textile bleaching ; dyeing ; printing ; etc ; TP890-933
Subject code	660
Language	English
Publishing date	2022-11-01T00:00:00Z
Publisher	Taylor & Francis Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Ligation-assisted endoscopic mucosal resection has high complete resection rate in rectal carcinoid tumor.

Su, Ming-Yao / Chiu, Cheng-Tang

BMC gastroenterology

2021 Volume 21, Issue 1, Page(s) 464

Abstract: Aim: We aimed to compare the outcomes of different therapeutic modalities in rectal carcinoid tumors.: Method: We retrospectively collected 145 patients with rectal carcinoid tumors which were pathologically diagnosed from 2005/01/01 to 2016/12/31. ... ...

Abstract	Aim: We aimed to compare the outcomes of different therapeutic modalities in rectal carcinoid tumors. Method: We retrospectively collected 145 patients with rectal carcinoid tumors which were pathologically diagnosed from 2005/01/01 to 2016/12/31. We compared tumor size, complete resection rate and recurrent rate between different therapeutic modalities. Then, prospectively compared the treatment outcomes of 28 patients treated with ligation assisted endoscopic mucosal resection (LEMR) and 25 patients treated with endoscopic mucosal resection with cap (EMRC). Result: The mean size of tumors was 6.5 mm (1-25 mm), and the mean follow-up duration was 26 months (6-118 months). The therapeutic modalities included ligation-assisted endoscopic mucosal resection (LEMR) (25 tumors, 17%), endoscopic mucosal resection (EMR) (31 tumors, 21%), snare polypectomy (30 tumors, 21%), biopsy forceps removal (46 tumors, 32%) and surgical resection (13 tumors, 11%), including 6 tumors treated with transanal endoscopic microsurgery (TEM) method. In view of pathologically complete resection rate, LEMR was highest (100%), followed by surgical resection (85%). However, EMR only had 42% pathologically complete resection rate. Besides, LEMR and surgical resection had no local recurrence and significantly higher clinically complete resection rate, compared to other treatments. For the further prospective study, complete resection was noted in 28 (100%) patients in LEMR group and 13 (52%) patients in EMRC group. Conclusion: In the treatment of rectal carcinoid tumors, LEMR is safe and effective compared with traditional endoscopic treatments.
MeSH term(s)	Carcinoid Tumor/surgery ; Endoscopic Mucosal Resection ; Humans ; Intestinal Mucosa/surgery ; Prospective Studies ; Rectal Neoplasms/surgery ; Retrospective Studies ; Treatment Outcome
Language	English
Publishing date	2021-12-13
Publishing country	England
Document type	Journal Article
ISSN	1471-230X
ISSN (online)	1471-230X
DOI	10.1186/s12876-021-02061-4
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Design, Manufacture, and Characterization of a Critical-Sized Gradient Porosity Dual-Material Tibial Defect Scaffold.

Lee, Ming-Chan / Pan, Cheng-Tang / Chen, Wen-Fan / Lin, Meng-Chi / Shiue, Yow-Ling

Bioengineering (Basel, Switzerland)

2024 Volume 11, Issue 4

Abstract: This study proposed a composite tibia defect scaffold with radial gradient porosity, utilizing finite element analysis to assess stress in the tibial region with significant critical-sized defects. Simulations for scaffolds with different porosities were ...

Abstract	This study proposed a composite tibia defect scaffold with radial gradient porosity, utilizing finite element analysis to assess stress in the tibial region with significant critical-sized defects. Simulations for scaffolds with different porosities were conducted, designing an optimal tibia defect scaffold with radial gradient porosity for repairing and replacing critical bone defects. Radial gradient porosity scaffolds resulted in a more uniform stress distribution, reducing titanium alloy stiffness and alleviating stress shielding effects. The scaffold was manufactured using selective laser melting (SLM) technology with stress relief annealing to simplify porous structure fabrication. The study used New Zealand white rabbits' tibia defect sites as simulation parameters, reconstructing the 3D model and implanting the composite scaffold. Finite element analysis in ANSYS-Workbench simulated forces under high-activity conditions, analyzing stress distribution and strain. In the simulation, the titanium alloy scaffold bore a maximum stress of 122.8626 MPa, while the centrally encapsulated HAp material delivered 27.92 MPa. The design demonstrated superior structural strength, thereby reducing stress concentration. The scaffold was manufactured using SLM, and the uniform design method was used to determine a collection of optimum annealing parameters. Nanoindentation and compression tests were used to determine the influence of annealing on the elastic modulus, hardness, and strain energy of the scaffold.
Language	English
Publishing date	2024-03-25
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2746191-9
ISSN	2306-5354
ISSN	2306-5354
DOI	10.3390/bioengineering11040308
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Article ; Online: Funneliformis mosseae Inoculation Enhances Cucurbita pepo L. Plant Growth and Fruit Yield by Reshaping Rhizosphere Microbial Community Structure

Junsong Wang / Wenjiang Fu / Chenyu Sun / Shuai Cai / Cheng Tang

Diversity, Vol 14, Iss 932, p

2022 Volume 932

Abstract: Arbuscular mycorrhizal fungi (AMF) are essential components of the soil microbiome that can facilitate plant growth and enhance abiotic and biotic stress resistance. However, the mechanisms via which AMF inoculation influences Cucurbita pepo L. plant ... ...

Abstract	Arbuscular mycorrhizal fungi (AMF) are essential components of the soil microbiome that can facilitate plant growth and enhance abiotic and biotic stress resistance. However, the mechanisms via which AMF inoculation influences Cucurbita pepo L. plant growth and fruit yield remain unclear. Here, we conducted pot experiments to investigate bacterial and fungal community structure in the rhizosphere of C. pepo plants inoculated with Funneliformis mosseae (Nicoll. & Gerd.) Gerd. & Trappe based on 16S ribosomal RNA and internal transcribed spacer gene sequencing. The α-diversity of bacteria increased significantly following F. mosseae inoculation, whereas the α-diversity of fungi exhibited an opposite trend ( p < 0.01). The relative abundances of major bacterial phyla, Actinobacteria, Acidobacteria, and Chloroflexi, together with the fungal phylum Ascomycota, were all higher in inoculated samples than in uninoculated controls. F. mosseae inoculation led to remarkable enrichment of potentially beneficial taxa (e.g., Streptomyces , Sphingomonas , Lysobacter , and Trichoderma ), in stark contrast to depletion of fungal pathogens (e.g., Botryotrichum , Acremonium , Fusarium , and Plectosphaerella ). Pathways related to amino acid metabolism and antibiotic biosynthesis were upregulated by F. mosseae inoculation, whereas pathways involved in infectious diseases were downregulated. The results suggest that F. mosseae inoculation reshapes the rhizosphere microbiome, thereby augmenting C. pepo plant growth and fruit yield.
Keywords	Arbuscular mycorrhizal fungi ; rhizosphere microbiome ; bacterial community ; fungal community ; Biology (General) ; QH301-705.5
Subject code	580
Language	English
Publishing date	2022-10-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Facile Synthesis of Hollow Glass Microsphere Filled PDMS Foam Composites with Exceptional Lightweight, Mechanical Flexibility, and Thermal Insulating Property

Tian-Long Han / Bi-Fan Guo / Guo-Dong Zhang / Long-Cheng Tang

Molecules, Vol 28, Iss 2614, p

2023 Volume 2614

Abstract: The feature of low-density and thermal insulation properties of polydimethylsiloxane (PDMS) foam is one of the important challenges of the silicone industry seeking to make these products more competitive compared to traditional polymer foams. Herein, we ...

Abstract	The feature of low-density and thermal insulation properties of polydimethylsiloxane (PDMS) foam is one of the important challenges of the silicone industry seeking to make these products more competitive compared to traditional polymer foams. Herein, we report a green, simple, and low-cost strategy for synthesizing ultra-low-density porous silicone composite materials via Si-H cross-linking and foaming chemistry, and the sialylation-modified hollow glass microspheres (m-HM) were used to promote the HM/PDMS compatibility. Typically, the presence of 7.5 wt% m-HM decreases the density of pure foam from 135 mg/cm −3 to 104 mg/cm −3 without affecting the foaming reaction between Si-H and Si-OH and produces a stable porous structure. The optimized m-HM-modified PDMS foam composites showed excellent mechanical flexibility (unchanged maximum stress values at a strain of 70% after 100 compressive cycles) and good thermal insulation (from 150.0 °C to 52.1 °C for the sample with ~20 mm thickness). Our results suggest that the use of hollow microparticles is an effective strategy for fabricating lightweight, mechanically flexible, and thermal insulation PDMS foam composite materials for many potential applications.
Keywords	polydimethylsiloxane foam ; hollow glass microsphere ; silicone chemistry ; thermal insulation ; lightweight ; Organic chemistry ; QD241-441
Subject code	600
Language	English
Publishing date	2023-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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