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  1. AU="Chenxiang Xi"
  2. AU="Alatawi, Mohammed Naif"
  3. AU="Jacquemet, Elise"
  4. AU="Cappelleri, Joseph C"
  5. AU="Frank, Samuel A"
  6. AU="Srensen, Henrik Toft"
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  1. Article ; Online: Pan-Genomic and Transcriptomic Analyses of Marine Pseudoalteromonas agarivorans Hao 2018 Revealed Its Genomic and Metabolic Features

    Yuhao Jv / Chenxiang Xi / Yanqiu Zhao / Wei Wang / Yiling Zhang / Kai Liu / Wenlin Liu / Kai Shan / Chunlei Wang / Ruiwen Cao / Cunxi Dai / Yiting Jv / Wenxing Zhu / Haiyong Wang / Qiuxia He / Lujiang Hao

    Marine Drugs, Vol 20, Iss 248, p

    2022  Volume 248

    Abstract: The genomic and carbohydrate metabolic features of Pseudoalteromonas agarivorans Hao 2018 ( P. agarivorans Hao 2018) were investigated through pan-genomic and transcriptomic analyses, and key enzyme genes that may encode the process involved in its ... ...

    Abstract The genomic and carbohydrate metabolic features of Pseudoalteromonas agarivorans Hao 2018 ( P. agarivorans Hao 2018) were investigated through pan-genomic and transcriptomic analyses, and key enzyme genes that may encode the process involved in its extracellular polysaccharide synthesis were screened. The pan-genome of the P. agarivorans strains consists of a core-genome containing 2331 genes, an accessory-genome containing 956 genes, and a unique-genome containing 1519 genes. Clusters of Orthologous Groups analyses showed that P. agarivorans harbors strain-specifically diverse metabolisms, probably representing high evolutionary genome changes. The Kyoto Encyclopedia of Genes and Genomes and reconstructed carbohydrate metabolic pathways displayed that P. agarivorans strains can utilize a variety of carbohydrates, such as d -glucose, d -fructose, and d -lactose. Analyses of differentially expressed genes showed that compared with the stationary phase (24 h), strain P. agarivorans Hao 2018 had upregulated expression of genes related to the synthesis of extracellular polysaccharides in the logarithmic growth phase (2 h), and that the expression of these genes affected extracellular polysaccharide transport, nucleotide sugar synthesis, and glycosyltransferase synthesis. This is the first investigation of the genomic and metabolic features of P. agarivorans through pan-genomic and transcriptomic analyses, and these intriguing discoveries provide the possibility to produce novel marine drug lead compounds with high biological activity.
    Keywords Pseudoalteromonas agarivorans ; pan-genome ; genomic and metabolic features ; KEGG analysis ; transcriptome analysis ; Biology (General) ; QH301-705.5
    Subject code 570
    Language English
    Publishing date 2022-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Effect of Different Initial Fermentation pH on Exopolysaccharides Produced by Pseudoalteromonas agarivorans Hao 2018 and Identification of Key Genes Involved in Exopolysaccharide Synthesis via Transcriptome Analysis

    Yuhao Ju / Kai Shan / Wenlin Liu / Chenxiang Xi / Yiling Zhang / Wei Wang / Chunlei Wang / Ruiwen Cao / Wenxing Zhu / Haiyong Wang / Yanqiu Zhao / Lujiang Hao

    Marine Drugs, Vol 20, Iss 89, p

    2022  Volume 89

    Abstract: Exopolysaccharides (EPSs) are carbohydrate polymers produced and secreted by microorganisms. In a changing marine environment, EPS secretion can reduce damage from external environmental disturbances to microorganisms. Meanwhile, EPSs have promising ... ...

    Abstract Exopolysaccharides (EPSs) are carbohydrate polymers produced and secreted by microorganisms. In a changing marine environment, EPS secretion can reduce damage from external environmental disturbances to microorganisms. Meanwhile, EPSs have promising application prospects in the fields of food, cosmetics, and pharmaceuticals. Changes in external environmental pH have been shown to affect the synthesis of EPSs in microorganisms. In this study, we analyzed the effects of different initial fermentation pHs on the production, monosaccharide composition, and antioxidant activity of the EPSs of Pseudoalteromonas agarivorans Hao 2018. In addition, the transcriptome sequence of P. agarivorans Hao 2018 under different initial fermentation pH levels was determined. GO and KEGG analyses showed that the differentially expressed genes were concentrated in the two-component regulatory system and bacterial chemotaxis pathways. We further identified the expression of key genes involved in EPS synthesis during pH changes. In particular, the expression of genes encoding the glucose/galactose MFS transporter, phosphomannomutase, and mannose-1-phosphate guanylyltransferase was upregulated when the environmental pH increased, thus promoting EPS synthesis. This study not only contributes to elucidating the environmental adaptation mechanisms of P. agarivorans , but also provides important theoretical guidance for the directed development of new products using biologically active polysaccharides.
    Keywords marine bacteria ; structural analysis ; transcriptome sequence ; KEGG analysis ; GO analysis ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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