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  1. Article ; Online: A Graphene-Coated Silicon Wafer Plate Improves the Sensitivity and Reproducibility of MALDI-TOF MS Analysis of Proteins.

    Choi, Yoon Kyung / Cheon, Dong Huey / Yang, Won Suk / Baek, Je-Hyun

    Journal of the American Society for Mass Spectrometry

    2023  Volume 34, Issue 9, Page(s) 2034–2042

    Abstract: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is widely used to analyze small and large molecules. However, proteins are difficult to analyze with MALDI-TOF MS in clinical applications because of their low ... ...

    Abstract Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is widely used to analyze small and large molecules. However, proteins are difficult to analyze with MALDI-TOF MS in clinical applications because of their low ionization efficiency and heterogeneous crystallization with the matrix on the sample spots. Here, we investigate the potential of a customized graphene-coated silicon wafer (G/SiO
    MeSH term(s) Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods ; Graphite ; Silicon ; Reproducibility of Results ; Silicon Dioxide ; Proteins
    Chemical Substances Graphite (7782-42-5) ; Silicon (Z4152N8IUI) ; Silicon Dioxide (7631-86-9) ; Proteins
    Language English
    Publishing date 2023-08-04
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1073671-2
    ISSN 1879-1123 ; 1044-0305
    ISSN (online) 1879-1123
    ISSN 1044-0305
    DOI 10.1021/jasms.3c00201
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  2. Article ; Online: Simultaneous Quantification of Apolipoprotein C-III

    Kim, Hyojin / Cheon, Dong Huey / Yang, Won Suk / Baek, Je-Hyun

    Journal of proteome research

    2022  Volume 22, Issue 1, Page(s) 91–100

    Abstract: Apolipoprotein C-III (APOC-III) regulates triglyceride levels, associated with a risk of cardiovascular disease. One gene generates several proteoforms, each with a different molecular mass and a unique function. Unlike peptide multiple reaction ... ...

    Abstract Apolipoprotein C-III (APOC-III) regulates triglyceride levels, associated with a risk of cardiovascular disease. One gene generates several proteoforms, each with a different molecular mass and a unique function. Unlike peptide multiple reaction monitoring (MRM), protein-MRM without digestion is required to analyze clinically relevant individual proteoforms. We developed a protein-MRM method without digestion to individually quantify APOC-III proteoforms in human serum. We optimized the protein-MRM method following 60% acetonitrile extraction with C18 filtration. Bovine serum and myoglobin served as supporting cushions and the internal standard during sample preparation, respectively. Furthermore, we evaluated the LOD, lower limit of quantification, linearity, accuracy, and precision. Good correlation compared with turbidimetric immunoassay (TIA) and peptide-MRM was observed using 30 clinical sera. Individual APOC-III
    MeSH term(s) Humans ; Apolipoprotein C-III/metabolism ; Cardiovascular Diseases/diagnosis ; Proteins ; Protein Processing, Post-Translational
    Chemical Substances Apolipoprotein C-III ; Proteins
    Language English
    Publishing date 2022-11-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2078618-9
    ISSN 1535-3907 ; 1535-3893
    ISSN (online) 1535-3907
    ISSN 1535-3893
    DOI 10.1021/acs.jproteome.2c00490
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  3. Article ; Online: Clinical Performance of the Osmotic Shock-MALDI MS Method to Detect Klebsiella pneumoniae Carbapenemase in Clinical Isolates.

    Lee, Saeyoung / Hwang, Seohyun / Cheon, Dong Huey / Jang, Heejung / Yang, Won Suk / Kim, Min Jin / Lee, SunHwa / Baek, Je-Hyun

    Journal of clinical microbiology

    2022  Volume 60, Issue 11, Page(s) e0106222

    Abstract: The World Health Organization recently highlighted the serious worldwide problem of the emergence of antibiotic-resistant or antibiotic multidrug-resistant bacteria. Carbapenem-resistant Enterobacterales, including carbapenemase-producing ... ...

    Abstract The World Health Organization recently highlighted the serious worldwide problem of the emergence of antibiotic-resistant or antibiotic multidrug-resistant bacteria. Carbapenem-resistant Enterobacterales, including carbapenemase-producing Enterobacterales (CPE), are major antibiotic-resistant bacteria that can be identified by various methods, including antibiotic susceptibility testing, PCR, and immunologic assays. However, there is a need for a faster, more accurate, low-cost, and easy method to detect CPE strains. We previously developed an osmotic shock matrix-assisted laser desorption/ionization mass spectrometry (OS-MALDI MS) method for directly detecting intact Klebsiella pneumoniae carbapenemase (KPC) using osmotic shock cell lysis. In this study, we evaluated the OS-MALDI MS method and compared it with two other methods (octyl-glucoside-aided direct KPC detection method [OG-MALDI MS] and Bruker's MBT subtyping module indirect method [MBT-SM MALDI MS]). We first completed an analytical performance evaluation of the OS-MALDI MS method according to Clinical and Laboratory Standards Institute guidelines. Clinical testing was performed with 437 clinical isolates, including 292 KPC-producing bacteria and 145 non-KPC-producing bacteria. The OS-MALDI MS method exhibited 95.9% sensitivity, 100.0% specificity, and 100.0% precision for detecting KPC. Accuracy of the OS-MALDI MS, OG-MALDI MS, and MBT-SM MALDI MS methods was 97.3%, 55.9%, and 50.2%, respectively. In conclusion, the OS-MALDI MS method clearly outperformed the other methods, exhibiting the highest accuracy and sensitivity of the three methods. We propose the OS-MALDI MS method as a practical, useful method for clinic environments, which may help guide appropriate antibiotic treatment and contribute to the prevention of the spread of CPE.
    MeSH term(s) Humans ; Klebsiella pneumoniae ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods ; Osmotic Pressure ; beta-Lactamases ; Bacterial Proteins ; Anti-Bacterial Agents/pharmacology ; Microbial Sensitivity Tests
    Chemical Substances carbapenemase (EC 3.5.2.6) ; beta-Lactamases (EC 3.5.2.6) ; Bacterial Proteins ; Anti-Bacterial Agents
    Language English
    Publishing date 2022-11-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 390499-4
    ISSN 1098-660X ; 0095-1137
    ISSN (online) 1098-660X
    ISSN 0095-1137
    DOI 10.1128/jcm.01062-22
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  4. Article ; Online: Optimization of a lysis method to isolate periplasmic proteins from Gram-negative bacteria for clinical mass spectrometry.

    Cheon, Dong Huey / Lee, Saeyoung / Yang, Won Suk / Hwang, Seohyun / Jang, Heejung / Kim, Min Jin / Baek, Je-Hyun

    Proteomics. Clinical applications

    2021  Volume 15, Issue 6, Page(s) e2100044

    Abstract: Purpose: Clinical mass spectrometry requires a simple step process for sample preparation. This study aims to optimize the method for isolating periplasmic protein from Gram-negative bacteria and apply to clinical mass spectrometry.: Experimental ... ...

    Abstract Purpose: Clinical mass spectrometry requires a simple step process for sample preparation. This study aims to optimize the method for isolating periplasmic protein from Gram-negative bacteria and apply to clinical mass spectrometry.
    Experimental design: The Klebsiella pneumoniae carbapenemase (KPC)-producing E. coli standard cells were used for optimizing the osmotic shock (OS) lysis method. The supernatant from OS lysis was analysed by LC-MS/MS and MALDI-TOF MS. The effectiveness of the OS lysis method for KPC-2-producing Enterobacteriaceae clinical isolates were then confirmed by MALDI-TOF MS.
    Results: The optimized OS lysis using KPC-2 producing E. coli standard cells showed a high yield of KPC-2 protein and enriches periplasmic proteins. Compared with other lysis methods, the detection sensitivity of KPC-2 protein significantly increased in MALDI-TOF MS analysis. Nineteen clinical isolates were validated by MALDI-TOF MS using the OS method, which also showed higher detection sensitivity compared to other lysis method (e.g., 1.5% n-octyl-β-D-glucopyranoside) (p < 0.001).
    Conclusions and clinical relevance: This study provides a straightforward, rapid, affordable, and detergent-free method for the analysis of periplasmic proteins from Enterobacteriaceae clinical isolates. This approach may contribute to MS-based clinical diagnostics.
    MeSH term(s) Chromatography, High Pressure Liquid ; Escherichia coli/enzymology ; Escherichia coli/isolation & purification ; Escherichia coli/metabolism ; Osmotic Pressure ; Periplasmic Proteins/analysis ; Periplasmic Proteins/isolation & purification ; Periplasmic Proteins/metabolism ; Sodium Chloride/chemistry ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; beta-Lactamases/metabolism
    Chemical Substances Periplasmic Proteins ; Sodium Chloride (451W47IQ8X) ; beta-lactamase KPC-2 (EC 3.5.2.-) ; beta-Lactamases (EC 3.5.2.6)
    Language English
    Publishing date 2021-08-18
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2261788-7
    ISSN 1862-8354 ; 1862-8346
    ISSN (online) 1862-8354
    ISSN 1862-8346
    DOI 10.1002/prca.202100044
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  5. Article ; Online: Low-Molecular-Weight Plasma Proteome Analysis Using Top-Down Mass Spectrometry.

    Cheon, Dong Huey / Yang, Eun Gyeong / Lee, Cheolju / Lee, Ji Eun

    Methods in molecular biology (Clifton, N.J.)

    2017  Volume 1619, Page(s) 103–117

    Abstract: While human plasma has a wealth of diagnostic information regarding the state of the human body in heath and disease, low molecular weight (LMW) proteome (<30 kDa) has been shown to contain a rich source of diagnostic biomarkers. Here we describe a ... ...

    Abstract While human plasma has a wealth of diagnostic information regarding the state of the human body in heath and disease, low molecular weight (LMW) proteome (<30 kDa) has been shown to contain a rich source of diagnostic biomarkers. Here we describe a protocol for top-down proteomic analysis to identify and characterize the LMW proteoforms present in four types of human plasma samples without immunoaffinity depletion and with depletion of the top two, six, and seven high-abundance proteins. Each type of plasma sample was first fractionated based on molecular weight using gel-eluted liquid fraction entrapment electrophoresis (GELFrEE). Then, the GELFrEE fractions containing up to 30 kDa were subjected to nanocapillary-LC-MS/MS, and the high-resolution MS and MS/MS data were processed using ProSightPC software. As a result, a total of 442 LMW proteins and cleaved products, including those with posttranslational modifications (PTMs) and single amino acid variations (SAAVs), were identified with a threshold E-value of 1 × 10
    MeSH term(s) Blood Proteins/chemistry ; Chemical Fractionation ; Chromatography, Liquid ; Molecular Weight ; Protein Processing, Post-Translational ; Proteome ; Proteomics/methods ; Tandem Mass Spectrometry
    Chemical Substances Blood Proteins ; Proteome
    Language English
    Publishing date 2017
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-7057-5_8
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  6. Article ; Online: Antibody Microarray Analysis of Plasma Proteins for the Prediction of Histologic Chorioamnionitis in Women With Preterm Premature Rupture of Membranes.

    Park, Jeong Woo / Park, Kyo Hoon / Lee, Ji Eun / Kim, Yu Mi / Lee, Se Jin / Cheon, Dong Huey

    Reproductive sciences (Thousand Oaks, Calif.)

    2019  Volume 26, Issue 11, Page(s) 1476–1484

    Abstract: We aimed to identify maternal blood biomarkers predictive of histologic chorioamnionitis (HCA) in the plasma of women with preterm premature rupture of membranes (PPROM) and to determine whether the combination of these biomarkers with conventional ... ...

    Abstract We aimed to identify maternal blood biomarkers predictive of histologic chorioamnionitis (HCA) in the plasma of women with preterm premature rupture of membranes (PPROM) and to determine whether the combination of these biomarkers with conventional clinical variables can improve the prediction of HCA. This retrospective cohort study included 82 consecutive women with PPROM (23-34 gestational weeks) who delivered within 96 hours of blood sampling. A membrane-based human antibody microarray was used to analyze the plasma proteome. The validation of 5 candidate biomarkers of interest was performed by enzyme-linked immunosorbent assay (ELISA) in the final cohort (n = 82). Serum C-reactive protein (CRP) levels were measured at sampling. Seventy-nine molecules studied exhibited intergroup differences. Validation by ELISA confirmed higher levels of plasma matrix metalloproteinase-9 (MMP-9), interleukin-6 (IL-6), S100 A8/A9, and insulin-like growth factor-binding protein 1 (IGFBP-1), but not tissue inhibitor of metalloproteinase 1 (TIMP-1), in women with HCA than in women without HCA. Using a stepwise regression analysis, a combined prediction model was developed, which included the plasma MMP-9, serum CRP levels, and gestational age (area under the curve [AUC], 0.932). The AUC for this model was significantly greater than that for any single variable included in the predictive model. Protein-antibody microarray technology can be useful in identifying plasma-based predictors for HCA. This study suggests that plasma MMP-9, IL-6, IGFBP-1, and S100 A8/A9 are important noninvasive predictors for HCA in women with PPROM and that the best predictive model, which combined these biomarkers with conventional clinical factors, can significantly improve the predictability for HCA.
    MeSH term(s) Adult ; Autoantibodies/blood ; Autoantibodies/genetics ; Biomarkers/blood ; Biomarkers/metabolism ; Blood Proteins/genetics ; Blood Proteins/metabolism ; Chorioamnionitis/blood ; Chorioamnionitis/diagnosis ; Chorioamnionitis/genetics ; Cohort Studies ; Female ; Fetal Membranes, Premature Rupture/blood ; Fetal Membranes, Premature Rupture/diagnosis ; Fetal Membranes, Premature Rupture/genetics ; Humans ; Microarray Analysis/methods ; Predictive Value of Tests ; Pregnancy ; Retrospective Studies
    Chemical Substances Autoantibodies ; Biomarkers ; Blood Proteins
    Language English
    Publishing date 2019-02-06
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2276411-2
    ISSN 1933-7205 ; 1933-7191
    ISSN (online) 1933-7205
    ISSN 1933-7191
    DOI 10.1177/1933719119828043
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  7. Article ; Online: Processing of syndecan-2 by matrix metalloproteinase-14 and effect of its cleavage on VEGF-induced tube formation of HUVECs.

    Lee, Young Hun / Park, Jun Hyoung / Cheon, Dong Huey / Kim, Taeyoung / Park, Yae Eun / Oh, Eok-Soo / Lee, Ji Eun / Lee, Seung-Taek

    The Biochemical journal

    2017  Volume 474, Issue 22, Page(s) 3719–3732

    Abstract: Syndecans (SDCs) are transmembrane proteoglycans that are involved in cell adhesion and cell communication. Specifically, SDC2 plays a key role in tumorigenesis, metastasis, and angiogenesis. Previously, we found that rat SDC2 is shed by matrix ... ...

    Abstract Syndecans (SDCs) are transmembrane proteoglycans that are involved in cell adhesion and cell communication. Specifically, SDC2 plays a key role in tumorigenesis, metastasis, and angiogenesis. Previously, we found that rat SDC2 is shed by matrix metalloproteinase-7 (MMP-7) in colon cancer cells. Here, we analyzed the susceptibility of rat SDC2 to various MMPs. We found that the rat SDC2 ectodomain (ECD) fused to the C-terminal Fc region, which was expressed in mammalian cells, was cleaved more efficiently by MMP-14 than MMP-7. Likewise, when anchored on the surface of HeLa cells, rat SDC2 was cleaved more efficiently by the treatment of MMP-14 than MMP-7 and was shed more readily by membrane-anchored MMP-14 than soluble MMP-14. Furthermore, MMP-14 cleaved recombinant SDC2-ECD expressed in
    MeSH term(s) Animals ; COS Cells ; Cercopithecus aethiops ; Dose-Response Relationship, Drug ; HeLa Cells ; Human Umbilical Vein Endothelial Cells/drug effects ; Human Umbilical Vein Endothelial Cells/physiology ; Humans ; Insecta ; Matrix Metalloproteinase 14/metabolism ; Matrix Metalloproteinase 14/pharmacology ; Rats ; Syndecan-2/biosynthesis ; Vascular Endothelial Growth Factor A/pharmacology
    Chemical Substances Sdc2 protein, rat ; VEGFA protein, human ; Vascular Endothelial Growth Factor A ; Syndecan-2 (149769-25-5) ; MMP14 protein, human (EC 3.4.24.80) ; Matrix Metalloproteinase 14 (EC 3.4.24.80)
    Language English
    Publishing date 2017-11-01
    Publishing country England
    Document type Journal Article
    ZDB-ID 2969-5
    ISSN 1470-8728 ; 0006-2936 ; 0306-3275 ; 0264-6021
    ISSN (online) 1470-8728
    ISSN 0006-2936 ; 0306-3275 ; 0264-6021
    DOI 10.1042/BCJ20170340
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  8. Article: Circulating MicroRNA Expression Levels Associated With Internet Gaming Disorder.

    Lee, Minho / Cho, Hyeyoung / Jung, Seung Hyun / Yim, Seon-Hee / Cho, Sung-Min / Chun, Ji-Won / Paik, Soo-Hyun / Park, Yae Eun / Cheon, Dong Huey / Lee, Ji Eun / Choi, Jung-Seok / Kim, Dai-Jin / Chung, Yeun-Jun

    Frontiers in psychiatry

    2018  Volume 9, Page(s) 81

    Abstract: Background: Addictive use of the Internet and online games is a potential psychiatric disorder termed Internet gaming disorder (IGD). Altered microRNA (miRNA) expression profiles have been reported in blood and brain tissue of patients with certain ... ...

    Abstract Background: Addictive use of the Internet and online games is a potential psychiatric disorder termed Internet gaming disorder (IGD). Altered microRNA (miRNA) expression profiles have been reported in blood and brain tissue of patients with certain psychiatric disorders and suggested as biomarkers. However, there have been no reports on blood miRNA profiles in IGD.
    Methods: To discover IGD-associated miRNAs, we analyzed the miRNA expression profiles of 51 samples (25 IGD and 26 controls) using the TaqMan Low Density miRNA Array. For validation, we performed quantitative reverse transcription PCR with 36 independent samples (20 IGD and 16 controls).
    Results: Through discovery and independent validation, we identified three miRNAs (hsa-miR-200c-3p, hsa-miR-26b-5p, hsa-miR-652-3p) that were significantly downregulated in the IGD group. Individuals with all three miRNA alterations had a much higher risk of IGD than those with no alteration [odds ratio (OR) 22, 95% CI 2.29-211.11], and the ORs increased dose dependently with number of altered miRNAs. The predicted target genes of the three miRNAs were associated with neural pathways. We explored the protein expression of the three downstream target genes by western blot and confirmed that expression of GABRB2 and DPYSL2 was significantly higher in the IGD group.
    Conclusion: We observed that expressions of hsa-miR-200c-3p, hsa-miR-26b-5p, and hsa-miR-652-3p were downregulated in the IGD patients. Our results will be helpful to understand the pathophysiology of IGD.
    Language English
    Publishing date 2018-03-12
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2564218-2
    ISSN 1664-0640
    ISSN 1664-0640
    DOI 10.3389/fpsyt.2018.00081
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  9. Article: Comprehensive Analysis of Low-Molecular-Weight Human Plasma Proteome Using Top-Down Mass Spectrometry

    Cheon, Dong Huey / Nam Eun Ji / Park Kyu Hyung / Woo Se Joon / Lee Hye Jin / Kim Hee Cheol / Yang Eun Gyeong / Lee Cheolju / Lee Ji Eun

    Journal of Proteome Research. 2016 Jan. 04, v. 15, no. 1

    2016  

    Abstract: While human plasma serves as a great source for disease diagnosis, low-molecular-weight (LMW) proteome (<30 kDa) has been shown to contain a rich source of diagnostic biomarkers. Here we employ top-down mass spectrometry to analyze the LMW proteoforms ... ...

    Abstract While human plasma serves as a great source for disease diagnosis, low-molecular-weight (LMW) proteome (<30 kDa) has been shown to contain a rich source of diagnostic biomarkers. Here we employ top-down mass spectrometry to analyze the LMW proteoforms present in four types of human plasma samples pooled from three healthy controls (HCs) without immunoaffinity depletion and with depletion of the top two, six, and seven high-abundance proteins. The LMW proteoforms were first fractionated based on molecular weight using gel-eluted liquid fraction entrapment electrophoresis (GELFrEE). Then, the GELFrEE fractions containing up to 30 kDa were subjected to nanocapillary–LC–MS/MS, and the high-resolution MS and MS/MS data were processed using ProSightPC 3.0. As a result, a total of 442 LMW proteins and cleaved products, including those with post-translational modifications and single amino acid variations, were identified. From additional comparative analysis of plasma samples without immunoaffinity depletion between HCs and colorectal cancer (CRC) patients via top-down approach, tens of LMW proteoforms, including platelet factor 4, were found to show >1.5-fold changes between the plasma samples of HCs and CRC patients, and six of the LMW proteins were verified by Western blot analysis.
    Keywords Western blotting ; amino acids ; biomarkers ; chemokine CXCL4 ; colorectal neoplasms ; disease diagnosis ; electrophoresis ; humans ; liquids ; mass spectrometry ; molecular weight ; patients ; post-translational modification ; proteins ; proteome
    Language English
    Dates of publication 2016-0104
    Size p. 229-244.
    Publishing place American Chemical Society
    Document type Article
    ZDB-ID 2078618-9
    ISSN 1535-3907 ; 1535-3893
    ISSN (online) 1535-3907
    ISSN 1535-3893
    DOI 10.1021%2Facs.jproteome.5b00773
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  10. Article ; Online: Effect of two lipid-lowering strategies on high-density lipoprotein function and some HDL-related proteins: a randomized clinical trial.

    Lee, Chan Joo / Choi, Seungbum / Cheon, Dong Huey / Kim, Kyeong Yeon / Cheon, Eun Jeong / Ann, Soo-Jin / Noh, Hye-Min / Park, Sungha / Kang, Seok-Min / Choi, Donghoon / Lee, Ji Eun / Lee, Sang-Hak

    Lipids in health and disease

    2017  Volume 16, Issue 1, Page(s) 49

    Abstract: Background: The influence of lipid-lowering therapy on high-density lipoprotein (HDL) is incompletely understood. We compared the effect of two lipid-lowering strategies on HDL functions and identified some HDL-related proteins.: Methods: Thirty two ... ...

    Abstract Background: The influence of lipid-lowering therapy on high-density lipoprotein (HDL) is incompletely understood. We compared the effect of two lipid-lowering strategies on HDL functions and identified some HDL-related proteins.
    Methods: Thirty two patients were initially screened and HDLs of 21 patients were finally analyzed. Patients were randomized to receive atorvastatin 20 mg (n = 11) or atorvastatin 5 mg/ezetimibe 10 mg combination (n = 10) for 8 weeks. The cholesterol efflux capacity and other anti-inflammatory functions were assessed based on HDLs of the participants before and after treatment. Pre-specified HDL proteins of the same HDL samples were measured.
    Results: The post-treatment increase in cholesterol efflux capacities was similar between the groups (35.6% and 34.6% for mono-therapy and combination, respectively, p = 0.60). Changes in nitric oxide (NO) production, vascular cell adhesion molecule-1 (VCAM-1) expression, and reactive oxygen species (ROS) production were similar between the groups. The baseline cholesterol efflux capacity correlated positively with apolipoprotein (apo)A1 and C3, whereas apoA1 and apoC1 showed inverse associations with VCAM-1 expression. The changes in the cholesterol efflux capacity were positively correlated with multiple HDL proteins, especially apoA2.
    Conclusions: Two regimens increased the cholesterol efflux capacity of HDL comparably. Multiple HDL proteins, not limited to apoA1, showed a correlation with HDL functions. These results indicate that conventional lipid therapy may have additional effects on HDL functions with changes in HDL proteins.
    Trial registration: ClinicalTrials.gov, number NCT02942602 .
    MeSH term(s) Anticholesteremic Agents/pharmacology ; Anticholesteremic Agents/therapeutic use ; Apolipoproteins/blood ; Atorvastatin Calcium/pharmacology ; Atorvastatin Calcium/therapeutic use ; Cholesterol, HDL/blood ; Drug Therapy, Combination ; Ezetimibe/pharmacology ; Ezetimibe/therapeutic use ; Female ; Humans ; Hypercholesterolemia/blood ; Hypercholesterolemia/drug therapy ; Male ; Middle Aged ; Treatment Outcome ; Vascular Cell Adhesion Molecule-1/blood
    Chemical Substances Anticholesteremic Agents ; Apolipoproteins ; Cholesterol, HDL ; Vascular Cell Adhesion Molecule-1 ; Atorvastatin Calcium (48A5M73Z4Q) ; Ezetimibe (EOR26LQQ24)
    Language English
    Publishing date 2017-02-28
    Publishing country England
    Document type Comparative Study ; Journal Article ; Randomized Controlled Trial
    ISSN 1476-511X
    ISSN (online) 1476-511X
    DOI 10.1186/s12944-017-0433-6
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    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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