LIVIVO - Search results -

Search results

Result 1 - 10 of total 11

Search options

Article ; Online: Glucose Oxidase Activity Colorimetric Assay Using Redox-Sensitive Electrochromic Nanoparticle-Functionalized Paper Sensors.

Lee, Taeha / Park, Jeongmin / Oh, Seung Hyeon / Cheong, Da Yeon / Roh, Seokbeom / You, Jae Hyun / Hong, Yoochan / Lee, Gyudo

ACS omega

2024 Volume 9, Issue 13, Page(s) 15493–15501

Abstract: Glucose oxidase (GOx) activity assays are vital for various applications, including glucose metabolism estimation and fungal testing. However, conventional methods involve time-consuming and complex procedures. In this study, we present a colorimetric ... ...

Abstract	Glucose oxidase (GOx) activity assays are vital for various applications, including glucose metabolism estimation and fungal testing. However, conventional methods involve time-consuming and complex procedures. In this study, we present a colorimetric platform for in situ GOx activity measurement utilizing redox-sensitive electrochromic nanoparticles based on polyaniline (PAni). The glucose-adsorbed colorimetric paper sensor, herein termed Glu@CPS, is created by immobilizing ferrocene and glucose onto paper substrates that have been functionalized with PAni nanoparticles. Glu@CPS not only demonstrated rapid detection (within 5 min) but also exhibited remarkable selectivity for GOx and a limit of detection as low as 1.25 μM. Moreover, Glu@CPS demonstrated consistent accuracy in the measurement of GOx activity, exhibiting no deviations even after being stored at ambient temperature for a duration of one month. To further corroborate the effectiveness of this method, we applied Glu@CPS in the detection of GOx activity in a moldy red wine. The results highlight the promising potential of Glu@CPS as a convenient and precise platform for GOx activity measurement in diverse applications including food quality control, environmental monitoring, and early detection of fungal contamination.
Language	English
Publishing date	2024-03-20
Publishing country	United States
Document type	Journal Article
ISSN	2470-1343
ISSN (online)	2470-1343
DOI	10.1021/acsomega.4c00335
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Direct observation of surface charge and stiffness of human metaphase chromosomes.

Roh, Seokbeom / Lee, Taeha / Cheong, Da Yeon / Kim, Yeonjin / Oh, Soohwan / Lee, Gyudo

Nanoscale advances

2022 Volume 5, Issue 2, Page(s) 368–377

Abstract: Metaphase chromosomes in which both polynucleotides and proteins are condensed with hierarchies are closely related to life phenomena such as cell division, cancer development, and cellular senescence. Nevertheless, their nature is rarely revealed, owing ...

Abstract	Metaphase chromosomes in which both polynucleotides and proteins are condensed with hierarchies are closely related to life phenomena such as cell division, cancer development, and cellular senescence. Nevertheless, their nature is rarely revealed, owing to their structural complexity and technical limitations in analytical methods. In this study, we used surface potential and nanomechanics mapping technology based on atomic force microscopy to measure the surface charge and intrinsic stiffness of metaphase chromosomes. We found that extra materials covering the chromosomes after the extraction process were positively charged. With the covering materials, the chromosomes were positively charged (
Language	English
Publishing date	2022-12-20
Publishing country	England
Document type	Journal Article
ISSN	2516-0230
ISSN (online)	2516-0230
DOI	10.1039/d2na00620k
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Amyloid Formation in Nanoliter Droplets.

Cheong, Da Yeon / Lee, Wonseok / Park, Insu / Park, Jinsung / Lee, Gyudo

International journal of molecular sciences

2022 Volume 23, Issue 10

Abstract: Processes that monitor the nucleation of amyloids and characterize the formation of amyloid fibrils are vital to medicine and pharmacology. In this study, we observe the nucleation and formation of lysozyme amyloid fibrils using a facile microfluidic ... ...

Abstract	Processes that monitor the nucleation of amyloids and characterize the formation of amyloid fibrils are vital to medicine and pharmacology. In this study, we observe the nucleation and formation of lysozyme amyloid fibrils using a facile microfluidic system to generate nanoliter droplets that can control the flow rate and movement of monomer-in-oil emulsion droplets in a T-junction microchannel. Using a fluorescence assay, we monitor the nucleation and growth process of amyloids based on the volume of droplets. Using the microfluidic system, we demonstrate that the lag phase, which is vital to amyloid nucleation and growth, is reduced at a lower droplet volume. Furthermore, we report a peculiar phenomenon of high amyloid formation at the edge of a bullet-shaped droplet, which is likely due to the high local monomer concentration. Moreover, we discovered that amyloid fibrils synthesized in the nanoliter droplets are shorter and thicker than fibrils synthesized from a bulk solution via the conventional heating method. Herein, a facile procedure to observe and characterize the nucleation and growth of amyloid fibrils using nanoliter droplets is presented, which is beneficial for investigating new features of amyloid fibril formation as an unconventional synthetic method for amyloid fibrils.
MeSH term(s)	Amyloid ; Amyloidogenic Proteins ; Emulsions ; Microfluidics
Chemical Substances	Amyloid ; Amyloidogenic Proteins ; Emulsions
Language	English
Publishing date	2022-05-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms23105480
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Hydrophobic Barriers for Directing

Lee, Taeha / Kang, Dain / Kim, Minsu / Choi, Sukyung / Cheong, Da Yeon / Roh, Seokbeom / Oh, Seung Hyeon / Park, Insu / Lee, Gyudo

ACS omega

2023 Volume 8, Issue 44, Page(s) 41649–41654

Abstract: ... Physarum ... ...

Abstract	Physarum polycephalum
Language	English
Publishing date	2023-10-25
Publishing country	United States
Document type	Journal Article
ISSN	2470-1343
ISSN (online)	2470-1343
DOI	10.1021/acsomega.3c05560
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Bioinspired lotus fiber-based graphene electronic textile for gas sensing

Cheong, Da Yeon / Lee, Sang Won / Park, Insu / Jung, Hyo Gi / Roh, Seokbeom / Lee, Dongtak / Lee, Taeha / Lee, Saebomeena / Lee, Wonseok / Yoon, Dae Sung / Lee, Gyudo

Cellulose. 2022 May, v. 29, no. 7

2022

Abstract: Graphene electronic textiles (e-textiles) have attracted significant attention in various sensing applications owing to their strong advantages. During the fabrication of these textiles, there are factors to consider, such as electrical conductivity, ... ...

Abstract	Graphene electronic textiles (e-textiles) have attracted significant attention in various sensing applications owing to their strong advantages. During the fabrication of these textiles, there are factors to consider, such as electrical conductivity, mechanical flexibility, weight, and applicability in other practical applications. Bioinspired lotus fiber has appropriate advantages to be used as graphene e-textiles, including lightweight (< 1 mg), eco-friendliness, crease-resistant, pilling resistance, and flexibility. However, lotus fiber-based graphene e-textiles have not yet been reported. In this study, we developed a reduced graphene oxide‒coated lotus fiber (RGOLF) which was successfully fabricated by the hydrogen interaction between graphene flakes and cellulose fiber. The higher the GO concentration (~ 3 g/L) and fiber diameter (~ 300 μm), the higher the electrical conductivity of the RGOLF was measured. The RGOLF exhibited a higher electrical conductivity (4.63 ± 0.22 μS) and a remarkable sensing performance for hazardous NO₂ gas molecules within a short exposure time (~ 3 min), including a low detection limit (~ 1 ppm), selectivity, and resistance to relative humidity. Moreover, we verified the mechanical flexibility and elasticity of RGOLF through a 1,000-cycle bending test, and tensile test, respectively. These results suggest that the bioinspired RGOLF could be used as a gas sensor in environmental air with a strong potential for use in various wearable applications.
Keywords	air ; cellulose ; cellulosic fibers ; detection limit ; electrical conductivity ; exposure duration ; fabrics ; graphene ; hydrogen ; relative humidity
Language	English
Dates of publication	2022-05
Size	p. 4071-4082.
Publishing place	Springer Netherlands
Document type	Article
ZDB-ID	1496831-9
ISSN	1572-882X ; 0969-0239
ISSN (online)	1572-882X
ISSN	0969-0239
DOI	10.1007/s10570-022-04541-6
Database	NAL-Catalogue (AGRICOLA)

Full text online

Accessible to users with ZB MED library card

Order via subito

Details ▾
- Full text online
- Order with fees

Article ; Online: Bioengineered amyloid peptide for rapid screening of inhibitors against main protease of SARS-CoV-2.

Lee, Dongtak / Jung, Hyo Gi / Park, Dongsung / Bang, Junho / Cheong, Da Yeon / Jang, Jae Won / Kim, Yonghwan / Lee, Seungmin / Lee, Sang Won / Lee, Gyudo / Kim, Yeon Ho / Hong, Ji Hye / Hwang, Kyo Seon / Lee, Jeong Hoon / Yoon, Dae Sung

Nature communications

2024 Volume 15, Issue 1, Page(s) 2108

Abstract: The coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has evoked a worldwide pandemic. As the emergence of variants has hampered the neutralization capacity of currently available vaccines, ... ...

Abstract	The coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has evoked a worldwide pandemic. As the emergence of variants has hampered the neutralization capacity of currently available vaccines, developing effective antiviral therapeutics against SARS-CoV-2 and its variants becomes a significant challenge. The main protease (M
MeSH term(s)	Humans ; SARS-CoV-2 ; COVID-19 ; Gold/pharmacology ; Protease Inhibitors/pharmacology ; Viral Nonstructural Proteins ; Metal Nanoparticles ; Peptides ; Peptide Hydrolases ; Antiviral Agents/pharmacology ; Molecular Docking Simulation
Chemical Substances	Gold (7440-57-5) ; Protease Inhibitors ; Viral Nonstructural Proteins ; Peptides ; Peptide Hydrolases (EC 3.4.-) ; Antiviral Agents
Language	English
Publishing date	2024-03-07
Publishing country	England
Document type	Journal Article
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-024-46296-7
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Dual regulatory effects of neferine on amyloid-β and tau aggregation studied by in silico, in vitro, and lab-on-a-chip technology.

Nam, Yunkwon / Prajapati, Ritu / Kim, Sujin / Shin, Soo Jung / Cheong, Da Yeon / Park, Yong Ho / Park, Hyun Ha / Lim, Danyou / Yoon, Yoojeong / Lee, Gyudo / Jung, Hyun Ah / Park, Insu / Kim, Dong-Hyun / Choi, Jae Sue / Moon, Minho

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

2024 Volume 172, Page(s) 116226

Abstract: Alzheimer's disease (AD) is characterized by the presence of two critical pathogenic factors: amyloid-β (Aβ) and tau. Aβ and tau become neurotoxic aggregates via self-assembly, and these aggregates contribute to the pathogenesis of AD. Therefore, there ... ...

Abstract	Alzheimer's disease (AD) is characterized by the presence of two critical pathogenic factors: amyloid-β (Aβ) and tau. Aβ and tau become neurotoxic aggregates via self-assembly, and these aggregates contribute to the pathogenesis of AD. Therefore, there has been growing interest in therapeutic strategies that simultaneously target Aβ and tau aggregates. Although neferine has attracted attention as a suitable candidate agent for alleviating AD pathology, there has been no study investigating whether neferine affects the modulation of Aβ or tau aggregation/dissociation. Herein, we investigated the dual regulatory effects of neferine on Aβ and tau aggregation/dissociation. We predicted the binding characteristics of neferine to Aβ and tau using molecular docking simulations. Next, thioflavin T and atomic force microscope analyses were used to evaluate the effects of neferine on the aggregation or dissociation of Aβ
MeSH term(s)	Humans ; Molecular Docking Simulation ; Amyloid beta-Peptides ; Alzheimer Disease/drug therapy ; Lab-On-A-Chip Devices ; Neurotoxicity Syndromes ; Technology ; Benzylisoquinolines
Chemical Substances	neferine (2292-16-2) ; Amyloid beta-Peptides ; Benzylisoquinolines
Language	English
Publishing date	2024-02-01
Publishing country	France
Document type	Journal Article
ZDB-ID	392415-4
ISSN	1950-6007 ; 0753-3322 ; 0300-0893
ISSN (online)	1950-6007
ISSN	0753-3322 ; 0300-0893
DOI	10.1016/j.biopha.2024.116226
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ud II Zs.198: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Colorimetric Nanoparticle-Embedded Hydrogels for a Biosensing Platform.

Lee, Taeha / Kim, Changheon / Kim, Jiyeon / Seong, Jung Bae / Lee, Youngjeon / Roh, Seokbeom / Cheong, Da Yeon / Lee, Wonseok / Park, Jinsung / Hong, Yoochan / Lee, Gyudo

Nanomaterials (Basel, Switzerland)

2022 Volume 12, Issue 7

Abstract: Hydrogels containing colorimetric nanoparticles have been used for ion sensing, glucose detection, and microbial metabolite analyses. In particular, the rapid chemical reaction owing to both the hydrogel form of water retention and the sensitive color ... ...

Abstract	Hydrogels containing colorimetric nanoparticles have been used for ion sensing, glucose detection, and microbial metabolite analyses. In particular, the rapid chemical reaction owing to both the hydrogel form of water retention and the sensitive color change of nanoparticles enables the rapid detection of target substances. Despite this advantage, the poor dispersibility of nanoparticles and the mechanical strength of nanoparticle-hydrogel complexes have limited their application. In this study, we demonstrate a milliliter agarose gel containing homogeneously synthesized polyaniline nanoparticles (PAni-NPs), referred to as PAni-NP-hydrogel complexes (PNHCs). To fabricate the optimal PNHC, we tested various pH solvents based on distilled water and phosphate-buffered saline and studied the colorimetric response of the PNHC with thickness. The colorimetric response of the prepared PNHC to the changes in the pH of the solution demonstrated excellent linearity, suggesting the possibility of using PNHC as a pH sensor. In addition, it was verified that the PNHC could detect minute pH changes caused by the cancer cell metabolites without cytotoxicity. Furthermore, the PNHC can be stably maintained outside water for approximately 12 h without deformation, indicating that it can be used as a disposable patch-type wearable biosensing platform.
Language	English
Publishing date	2022-03-30
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662255-5
ISSN	2079-4991
ISSN	2079-4991
DOI	10.3390/nano12071150
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: A regression-based machine learning approach for pH and glucose detection with redox-sensitive colorimetric paper sensors.

Lee, Taeha / Lee, Hyung-Tak / Hong, Jiho / Roh, Seokbeom / Cheong, Da Yeon / Lee, Kyungwon / Choi, Yeojin / Hong, Yoochan / Hwang, Han-Jeong / Lee, Gyudo

Analytical methods : advancing methods and applications

2022 Volume 14, Issue 46, Page(s) 4749–4755

Abstract: Colorimetric paper sensors are used in various fields due to their convenience and intuitive manner. However, these sensors present low accuracy in practical use because it is difficult to distinguish color changes for a minute amount of analyte with the ...

Abstract	Colorimetric paper sensors are used in various fields due to their convenience and intuitive manner. However, these sensors present low accuracy in practical use because it is difficult to distinguish color changes for a minute amount of analyte with the naked eye. Herein, we demonstrate that a machine learning (ML)-based paper sensor platform accurately determines the color changes. We fabricated a colorimetric paper sensor by adsorbing polyaniline nanoparticles (PAni-NPs), whose color changes from blue to green when the ambient pH decreases. Adding glucose oxidase (GOx) to the paper sensor enables colorimetric glucose detection. Target analytes (10 μL) were aliquoted onto the paper sensors, and their images were taken with a smartphone under the same conditions in a darkroom. The red-green-blue (RGB) data from the images were extracted and used to train and test three regression models: support vector regression (SVR), decision tree regression (DTR), and random forest regression (RFR). Of the three regression models, RFR performed the best at estimating pH levels (
MeSH term(s)	Colorimetry/methods ; Machine Learning ; Oxidation-Reduction ; Glucose ; Hydrogen-Ion Concentration
Chemical Substances	Glucose (IY9XDZ35W2)
Language	English
Publishing date	2022-12-01
Publishing country	England
Document type	Journal Article
ZDB-ID	2515210-5
ISSN	1759-9679 ; 1759-9660
ISSN (online)	1759-9679
ISSN	1759-9660
DOI	10.1039/d2ay01329k
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Proteolysis-driven proliferation and rigidification of pepsin-resistant amyloid fibrils.

Cheong, Da Yeon / Roh, Seokbeom / Park, Insu / Lin, Yuxi / Lee, Young-Ho / Lee, Taeha / Lee, Sang Won / Lee, Dongtak / Jung, Hyo Gi / Kim, Hyunji / Lee, Wonseok / Yoon, Dae Sung / Hong, Yoochan / Lee, Gyudo

International journal of biological macromolecules

2022 Volume 227, Page(s) 601–607

Abstract: Proteolysis of amyloids is related to prevention and treatment of amyloidosis. What if the conditions for proteolysis were the same to those for amyloid formation? For example, pepsin, a gastric protease is activated in an acidic environment, which, ... ...

Abstract	Proteolysis of amyloids is related to prevention and treatment of amyloidosis. What if the conditions for proteolysis were the same to those for amyloid formation? For example, pepsin, a gastric protease is activated in an acidic environment, which, interestingly, is also a condition that induces the amyloid formation. Here, we investigate the competition reactions between proteolysis and synthesis of amyloid under pepsin-activated conditions. The changes in the quantities and nanomechanical properties of amyloids after pepsin treatment were examined by fluorescence assay, circular dichroism and atomic force microscopy. We found that, in the case of pepsin-resistant amyloid, a secondary reaction can be accelerated, thereby proliferating amyloids. Moreover, after this reaction, the amyloid became 32.4 % thicker and 24.2 % stiffer than the original one. Our results suggest a new insight into the proteolysis-driven proliferation and rigidification of pepsin-resistant amyloids.
Language	English
Publishing date	2022-12-18
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2022.12.104
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.B 2374: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

To top

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito