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Article ; Online: A Theory of Change for Guiding the Integration of Human-Centered Design Into Global Health Programming.

LaFond, Anne / Cherney, Montana

2021 Volume 9, Issue Suppl 2, Page(s) S209–S216

Abstract: The goal of this theory of change is to provide funders, implementing partners, and designers a tool to increase the understanding and application of human-centered design (HCD) as an approach that enhances global health programming. The theory of change ...

Abstract	The goal of this theory of change is to provide funders, implementing partners, and designers a tool to increase the understanding and application of human-centered design (HCD) as an approach that enhances global health programming. The theory of change also aims to communicate the potential for HCD to introduce new and disruptive mindsets, practices, and techniques to global health programming and to clarify HCD's potential value from the funding application process through to implementation. We seek to answer the key question: how do design and traditional global health practices and mindsets integrate to drive more people-centered, innovative solutions to health challenges and achieve common health sector and global health ecosystem goals?
MeSH term(s)	Ecosystem ; Global Health ; Humans
Language	English
Publishing date	2021-11-29
Publishing country	United States
Document type	Journal Article
ZDB-ID	2710875-2
ISSN	2169-575X ; 2169-575X
ISSN (online)	2169-575X
ISSN	2169-575X
DOI	10.9745/GHSP-D-21-00334
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Article ; Online: Challenges and opportunities in diagnosing and managing mild traumatic brain injury in rural settings.

Daugherty, Jill / Waltzman, Dana / Popat, Shena / Horn Groenendaal, Amy / Cherney, Maggie / Knudson, Alana

Rural and remote health

2022 Volume 22, Issue 2, Page(s) 7241

Abstract: Introduction: There is some evidence to suggest that Americans living in rural areas are at increased risk for sustaining a traumatic brain injury (TBI) compared to those living in urban areas. In addition, once a TBI has been sustained, rural residents ...

Abstract	Introduction: There is some evidence to suggest that Americans living in rural areas are at increased risk for sustaining a traumatic brain injury (TBI) compared to those living in urban areas. In addition, once a TBI has been sustained, rural residents have worse outcomes, including a higher risk of death. Individuals living in rural areas tend to live farther from hospitals and have less access to TBI specialists. Aside from these factors, little is known what challenges healthcare providers practicing in rural areas face in diagnosing and managing TBI in their patients and what can be done to overcome these challenges. Methods: Seven focus groups and one individual interview were conducted with a total of 18 healthcare providers who mostly practiced in primary care or emergency department settings in rural areas. Providers were asked about common mechanisms of TBI in patients that they treat, challenges they face in initial and follow-up care, and opportunities for improvement in their practice. Results: The rural healthcare providers reported that common mechanisms of injury included sports-related injuries for their pediatric and adolescent patients and work-related accidents, motor vehicle crashes, and falls among their adult patients. Most providers felt prepared to diagnose and manage their patients with TBI, but acknowledged a series of challenges they face, including pushback from parents, athletes, and coaches and lack of specialists to whom they could refer. They also noted that patients had their own barriers to overcome for timely and adequate care, including lack of access to transportation, difficulties with cost and insurance, and denial about the seriousness of the injury. Despite these challenges, the focus group participants also outlined benefits to practicing in a rural area and several ways that their practice could improve with support. Conclusion: Rural healthcare providers may be comfortable diagnosing, treating, and managing their patients who present with a suspected TBI, but they also face many challenges in their practice. In this study it was continually noted that there was lack of resources and a lack of awareness, or recognition of the seriousness of TBI, among the providers' patient populations. Education about common symptoms and the need for evaluation after an injury is needed. The use of telemedicine, an increasingly common technology, may help close some gaps in access to services. People living in rural areas may be at increased risk for TBI. Healthcare providers who work in these areas face many challenges but have found ways to successfully manage the treatment of this injury in their patients.
MeSH term(s)	Accidents, Traffic ; Adolescent ; Adult ; Athletic Injuries ; Brain Concussion/diagnosis ; Brain Concussion/therapy ; Brain Injuries, Traumatic/diagnosis ; Brain Injuries, Traumatic/therapy ; Child ; Humans ; Rural Population ; United States
Language	English
Publishing date	2022-06-15
Publishing country	Australia
Document type	Journal Article
ZDB-ID	2105620-1
ISSN	1445-6354 ; 1445-6354
ISSN (online)	1445-6354
ISSN	1445-6354
DOI	10.22605/RRH7241
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Article ; Online: Rural Primary Care Providers' Experience and Usage of Clinical Recommendations in the CDC Pediatric Mild Traumatic Brain Injury Guideline: A Qualitative Study.

Daugherty, Jill / Waltzman, Dana / Popat, Shena / Groenendaal, Amy Horn / Cherney, Margaret / Knudson, Alana

The Journal of rural health : official journal of the American Rural Health Association and the National Rural Health Care Association

2020 Volume 37, Issue 3, Page(s) 487–494

Abstract: Purpose: In 2018, the Centers for Disease Control and Prevention (CDC) released an evidence-based guideline on pediatric mild traumatic brain injury (mTBI) to educate health care providers on best practices of mTBI diagnosis, prognosis, and management/ ... ...

Abstract	Purpose: In 2018, the Centers for Disease Control and Prevention (CDC) released an evidence-based guideline on pediatric mild traumatic brain injury (mTBI) to educate health care providers on best practices of mTBI diagnosis, prognosis, and management/treatment. As residents living in rural areas have higher rates of mTBI, and may have limited access to care, it is particularly important to disseminate the CDC guideline to rural health care providers. The purpose of this paper is to describe rural health care providers' experience with pediatric mTBI patients and their perceptions on incorporating the guideline recommendations into their practice. Method: Interviews with 9 pediatric rural health care providers from all US regions were conducted. Interview transcripts were coded and analyzed for themes for each of the main topic areas covered in the interview guide. Findings: Common causes of mTBI reported by health care providers included sports and all-terrain vehicles. While health care providers found the guideline recommendations to be helpful and feasible, they reported barriers to implementation, such as lack of access to specialists. To help with uptake of the CDC guideline, they suggested the development of concise implementation tools that can be referenced quickly, integrated into electronic health record-based systems, and that are customized by visit type and health care setting (eg, initial vs follow-up visits and emergency department vs primary care visits). Conclusion: Length, accessibility, and usability are important considerations when designing clinical tools for busy rural health care providers caring for pediatric patients with mTBI. Customized information, in both print and digital formats, may help with uptake of best practices.
MeSH term(s)	Brain Concussion ; Centers for Disease Control and Prevention, U.S. ; Child ; Health Personnel ; Humans ; Primary Health Care ; Qualitative Research ; United States
Language	English
Publishing date	2020-10-27
Publishing country	England
Document type	Journal Article
ZDB-ID	639160-6
ISSN	1748-0361 ; 0890-765X
ISSN (online)	1748-0361
ISSN	0890-765X
DOI	10.1111/jrh.12530
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Article ; Online: Insights into the Slow Uptake of Residential Lead Paint Remediation Funds: A Lancaster, Pennsylvania, Case Study.

Cherney, Margaret / Erdman, Sarabeth / Kuon, Madeline / Shupin, Nicholas / Regis, Najeda / Fitzelle-Jones, Emma / Givler, Kylie / Baldrige, Susan / Okatch, Harriet

International journal of environmental research and public health

2021 Volume 18, Issue 2

Abstract: Lead poisoning is a preventable condition that continues to affect thousands of children each year. Given that local governments and municipalities are eligible to apply for federal funds to perform lead remediation in low-income family homes, we sought ... ...

Abstract	Lead poisoning is a preventable condition that continues to affect thousands of children each year. Given that local governments and municipalities are eligible to apply for federal funds to perform lead remediation in low-income family homes, we sought to understand how lead poisoning knowledge levels may affect the uptake of these funds. We recruited and conducted 28 in-depth, semi-structured interviews with community members from Lancaster County in the state of Pennsylvania in the USA. We audio-recorded and transcribed each interview, and analyzed each transcript for salient themes. The interviewed participants displayed a varying degree of knowledge about lead and lead poisoning. Most of the participants were unaware of the lead paint remediation funds. Participants learned about lead from various sources, such as social media, and personal experiences with lead poisoning appeared to enhance knowledge. Some participants assumed lead poisoning prevention would be addressed by other stakeholders if necessary, including healthcare professionals and landlords. The results of this study suggest that in order to increase the timely uptake of the remediation funds, community-based organizations should design interventions that aim to increase awareness and knowledge about lead poisoning and lead poisoning prevention. These interventions should be tailored for different audiences including community members, healthcare professionals, and landlords.
MeSH term(s)	Child ; Cities ; Environmental Exposure ; Financial Management ; Humans ; Lead ; Lead Poisoning/prevention & control ; Paint ; Pennsylvania
Chemical Substances	Lead (2P299V784P)
Language	English
Publishing date	2021-01-14
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1660-4601
ISSN (online)	1660-4601
DOI	10.3390/ijerph18020652
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Article ; Online: Professionals' Perceptions: "Why is Lead Poisoning Prevalent in Lancaster County?"

Okatch, Harriet / Cherney, Margaret / Mokshefsky, Brittany / Kuon, Madeline / Scheuring, Sarah / Ritchey, Emily / Chen, Jiayi

International journal of environmental research and public health

2019 Volume 16, Issue 13

Abstract: Background: The prevalence of lead poisoning in children under the age of six years living in Lancaster County, Pennsylvania continues to be greater than the state-wide prevalence for this age group. This study aims to determine the factors that ... ...

Abstract	Background: The prevalence of lead poisoning in children under the age of six years living in Lancaster County, Pennsylvania continues to be greater than the state-wide prevalence for this age group. This study aims to determine the factors that contribute to the high lead poisoning rates. Methods: For this qualitative study, the researchers recruited a convenience sample of professionals providing healthcare and social welfare services in the county. Researchers conducted in-depth interviews with participants. The research team audio recorded, transcribed verbatim, and analyzed each interview using NVivo 12 software. Results: The 16 interviewed participants identified factors that contribute to high lead poisoning rates including knowledge levels, housing status, and competing interests. Specifically, low knowledge levels, renting as opposed to home ownership, and having competing interests seemed to minimize the attention directed towards preventing lead poisoning. The experts offered recommendations to address the high lead poisoning rates including increasing lead knowledge levels of both community members and landlords, through creating and distributing health promotion material, enacting policies to empower renters, and systematically collaborating to streamline lead poisoning related information and services. Conclusions: Findings provide insights to factors that Lancaster can address to achieve a decrease in lead poisoning rates. This study provides information that can be utilized by public health professionals to develop appropriate interventions.
MeSH term(s)	Child, Preschool ; Epidemiologic Studies ; Female ; Humans ; Infant ; Infant, Newborn ; Lead Poisoning/epidemiology ; Male ; Pennsylvania/epidemiology ; Prevalence ; Public Health/statistics & numerical data ; Qualitative Research
Language	English
Publishing date	2019-06-27
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1660-4601
ISSN (online)	1660-4601
DOI	10.3390/ijerph16132281
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Article ; Online: The relative role of CYP3A4 and CYP3A5 in eplerenone metabolism.

McGraw, Joseph / Cherney, Mitchell / Bichler, Katherine / Gerhardt, Armin / Nauman, Mirielle

Toxicology letters

2019 Volume 315, Page(s) 9–13

Abstract: Cytochrome P450 mediated metabolism is the rate-limiting step of elimination for many drugs. CYP3A4 is the most abundant hepatic isoform and CYP3A4/5 metabolize the largest fraction of drugs. Pharmacogenetic studies have not been able to characterize ... ...

Abstract	Cytochrome P450 mediated metabolism is the rate-limiting step of elimination for many drugs. CYP3A4 is the most abundant hepatic isoform and CYP3A4/5 metabolize the largest fraction of drugs. Pharmacogenetic studies have not been able to characterize population variability in CYP3A4 activity because few variant alleles associated with aberrant enzyme activity have been found. Substrate probes such as midazolam and testosterone have been utilized in-vivo and in-vitro to determine catalytic activity of these enzymes, but they suffer from several limitations. Eplerenone, an aldosterone antagonist, is also metabolized by CYP3A enzymes, and it has the potential to be an excellent substrate probe for CYP3A4/5. Eplerenone's primary metabolite, 6 beta-hydroxyeplerenone is formed preferentially via CYP3A4, however, the relative contribution of CYP3A5 to the 21-hydroxyeplerenone metabolite formation is unknown. Through in-vitro microsomal incubations with recombinant CYP3A4 and CYP3A5 enzymes, we identified their relative contributions to 21-hydroxyeplerenone metabolism. The 21-hydroxy metabolite is formed preferentially via CYP3A5 V
MeSH term(s)	Antihypertensive Agents/metabolism ; Antihypertensive Agents/pharmacology ; Antihypertensive Agents/therapeutic use ; Cytochrome P-450 CYP3A/metabolism ; Cytochrome P-450 Enzyme System/metabolism ; Cytochrome P-450 Enzyme System/pharmacology ; Eplerenone/metabolism ; Eplerenone/pharmacology ; Humans ; Hypertension/drug therapy ; Microsomes/metabolism
Chemical Substances	Antihypertensive Agents ; Eplerenone (6995V82D0B) ; Cytochrome P-450 Enzyme System (9035-51-2) ; Cytochrome P-450 CYP3A (EC 1.14.14.1)
Language	English
Publishing date	2019-08-10
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	433788-8
ISSN	1879-3169 ; 0378-4274
ISSN (online)	1879-3169
ISSN	0378-4274
DOI	10.1016/j.toxlet.2019.08.003
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Article ; Online: Structure of the N-terminal domain of the effector protein LegC3 from Legionella pneumophila.

Yao, Deqiang / Cherney, Maia / Cygler, Miroslaw

Acta crystallographica. Section D, Biological crystallography

2014 Volume 70, Issue Pt 2, Page(s) 436–441

Abstract: Legionella pneumophila secretes over 300 effectors during the invasion of human cells. The functions of only a small number of them have been identified. LegC3 is one of the identified effectors, which is believed to act by inhibiting vacuolar fusion. It ...

Abstract	Legionella pneumophila secretes over 300 effectors during the invasion of human cells. The functions of only a small number of them have been identified. LegC3 is one of the identified effectors, which is believed to act by inhibiting vacuolar fusion. It contains two predicted transmembrane helices that divide the protein into a larger N-terminal domain and a smaller C-terminal domain. The function of LegC3 has been shown to be associated primarily with the N-terminal domain, which contains coiled-coil sequence motifs. The structure of the N-terminal domain has been determined and it is shown that it is highly α-helical and contains a helical bundle followed by a long antiparallel coiled-coil. No similar protein fold has been observed in the PDB. A long loop at the tip of the coiled-coil distal from the membrane is disordered and may be important for interaction with an as yet unidentified protein.
MeSH term(s)	Amino Acid Motifs ; Bacterial Proteins/chemistry ; Bacterial Proteins/genetics ; Crystallography, X-Ray ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Legionella pneumophila/chemistry ; Legionella pneumophila/metabolism ; Models, Molecular ; Molecular Sequence Data ; Protein Folding ; Protein Structure, Secondary ; Protein Structure, Tertiary ; Recombinant Proteins/chemistry ; Recombinant Proteins/genetics
Chemical Substances	Bacterial Proteins ; Recombinant Proteins
Language	English
Publishing date	2014-02
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2020492-9
ISSN	1399-0047 ; 2059-7983 ; 0907-4449
ISSN (online)	1399-0047 ; 2059-7983
ISSN	0907-4449
DOI	10.1107/S139900471302991X
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Article ; Online: Quaternary structure of WzzB and WzzE polysaccharide copolymerases.

Kalynych, Sergei / Cherney, Maia / Bostina, Mihnea / Rouiller, Isabelle / Cygler, Miroslaw

Protein science : a publication of the Protein Society

2014 Volume 24, Issue 1, Page(s) 58–69

Abstract: Bacteria have evolved cellular control mechanisms to ensure proper length specification for surface-bound polysaccharides. Members of the Polysaccharide Copolymerase (PCP) family are central to this process. PCP-1 family members are anchored to the inner ...

Abstract	Bacteria have evolved cellular control mechanisms to ensure proper length specification for surface-bound polysaccharides. Members of the Polysaccharide Copolymerase (PCP) family are central to this process. PCP-1 family members are anchored to the inner membrane through two transmembrane helices and contain a large periplasm-exposed domain. PCPs are known to form homooligomers but their exact stoichiometry is controversial in view of conflicting structural and biochemical data. Several prior investigations addressing this question indicated a nonameric, hexameric, or tetrameric organization of several PCP-1 family members. In this work, we gathered additional evidence that E.coli WzzB and WzzE PCPs form octameric homo-oligomeric complexes. Detergent-solubilized PCPs were purified to homogeneity and subjected to blue native gel analysis, which indicated the presence of a predominant high-molecular product of over 500 kDa in mass. Molecular mass of WzzE and WzzB-detergent oligomers was estimated to be 550 kDA by size-exclusion coupled to multiangle laser light scattering (SEC-MALLS). Oligomeric organization of purified WzzB and WzzE was further investigated by negative stain electron microscopy and by X-ray crystallography, respectively. Analysis of EM-derived molecular envelope of WzzB indicated that the full-length protein is composed of eight protomers. Crystal structure of LDAO-solubilized WzzE was solved to 6 Å resolutions and revealed its octameric subunit stoichiometry. In summary, we identified a possible biological unit utilized for the glycan chain length determination by two PCP-1 family members. This provides an important step toward further unraveling of the mechanistic basis of chain length control of the O-antigen and the enterobacterial common antigen.
MeSH term(s)	Crystallography, X-Ray ; Escherichia coli/chemistry ; Escherichia coli Proteins/chemistry ; Models, Molecular ; Protein Multimerization ; Protein Structure, Quaternary
Chemical Substances	Escherichia coli Proteins ; wzzB protein, E coli
Language	English
Publishing date	2014-11-04
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1106283-6
ISSN	1469-896X ; 0961-8368
ISSN (online)	1469-896X
ISSN	0961-8368
DOI	10.1002/pro.2586
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Zs.A 3407: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: NleH defines a new family of bacterial effector kinases.

Grishin, Andrey M / Cherney, Maia / Anderson, Deborah H / Phanse, Sadhna / Babu, Mohan / Cygler, Miroslaw

Structure (London, England : 1993)

2014 Volume 22, Issue 2, Page(s) 250–259

Abstract: Upon host cell infection, pathogenic Escherichia coli hijacks host cellular processes with the help of 20-60 secreted effector proteins that subvert cellular processes to create an environment conducive to bacterial survival. The NleH effector kinases ... ...

Abstract	Upon host cell infection, pathogenic Escherichia coli hijacks host cellular processes with the help of 20-60 secreted effector proteins that subvert cellular processes to create an environment conducive to bacterial survival. The NleH effector kinases manipulate the NF-κB pathway and prevent apoptosis. They show low sequence similarity to human regulatory kinases and contain two domains, the N-terminal, likely intrinsically unfolded, and a C-terminal kinase-like domain. We show that these effectors autophosphorylate on sites located predominantly in the N-terminal segment. The kinase domain displays a minimal kinase fold, but lacks an activation loop and the GHI subdomain. Nevertheless, all catalytically important residues are conserved. ATP binding proceeds with minimal structural rearrangements. The NleH structure is the first for the bacterial effector kinases family. NleHs and their homologous effector kinases form a new kinase family within the cluster of eukaryotic-like kinases that includes also Rio, Bud32, and KdoK families.
MeSH term(s)	Adenosine Triphosphate/chemistry ; Amino Acid Sequence ; Apoptosis ; Catalysis ; Escherichia coli/enzymology ; Escherichia coli Proteins/chemistry ; Mass Spectrometry ; Molecular Sequence Data ; NF-kappa B/chemistry ; Phosphorylation ; Phosphotransferases/chemistry ; Protein Folding ; Protein Structure, Tertiary ; Recombinant Proteins/chemistry ; Sequence Homology, Amino Acid
Chemical Substances	Escherichia coli Proteins ; NF-kappa B ; NleH protein, E coli ; Recombinant Proteins ; Adenosine Triphosphate (8L70Q75FXE) ; Phosphotransferases (EC 2.7.-)
Language	English
Publishing date	2014-02-04
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1213087-4
ISSN	1878-4186 ; 0969-2126
ISSN (online)	1878-4186
ISSN	0969-2126
DOI	10.1016/j.str.2013.11.006
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Article: Dynamics of the His79-Heme Alkaline Transition of Yeast Iso-1-cytochrome c Probed by Conformationally Gated Electron Transfer with Co(II)bis(terpyridine)

Cherney, MelisaM / Junior, Carolyn C / Bergquist, BryanB / Bowler, Bruce E

Journal of the American Chemical Society. 2013 Aug. 28, v. 135, no. 34

2013

Abstract: Alkaline conformers of cytochrome c may be involved in both its electron transport and apoptotic functions. We use cobalt(II)bis(terpyridine), Co(terpy)22+, as a reagent for conformationally gated electron-transfer (gated ET) experiments to study the ... ...

Abstract	Alkaline conformers of cytochrome c may be involved in both its electron transport and apoptotic functions. We use cobalt(II)bis(terpyridine), Co(terpy)22+, as a reagent for conformationally gated electron-transfer (gated ET) experiments to study the alkaline conformational transition of K79H variants of yeast iso-1-cytochrome c expressed in Escherichia coli, WTK79H, with alanine at position 72 and Saccharomyces cerevisiae, yK79H, with trimethyllysine (Tml) at position 72. Co(terpy)22+ is well-suited to the 100 ms to 1 s time scale of the His79-mediated alkaline conformational transition of these variants. Reduction of the His79-heme alkaline conformer by Co(terpy)22+ occurs primarily by gated ET, which involves conversion to the native state followed by reduction, with a small fraction of the His79-heme alkaline conformer directly reduced by Co(terpy)22+. The gated ET experiments show that the mechanism of formation of the His79-heme alkaline conformer involves only two ionizable groups. In previous work, we showed that the mechanism of the His73-mediated alkaline conformational transition requires three ionizable groups. Thus, the mechanism of heme crevice opening depends upon the position of the ligand mediating the process. The microscopic rate constants provided by gated ET studies show that mutation of Tml72 (yK79H variant) in the heme crevice loop to Ala72 (WTK79H variant) affects the dynamics of heme crevice opening through a small destabilization of both the native conformer and the transition state relative to the His79-heme alkaline conformer. Previous pH jump data had indicated that the Tml72→Ala mutation primarily stabilized the transition state for the His79-mediated alkaline conformational transition.
Keywords	Escherichia coli ; Saccharomyces cerevisiae ; alanine ; apoptosis ; cobalt ; cytochrome c ; electron transfer ; heme ; ligands ; mutation ; pH ; yeasts
Language	English
Dates of publication	2013-0828
Size	p. 12772-12782.
Publishing place	American Chemical Society
Document type	Article
ZDB-ID	3155-0
ISSN	1520-5126 ; 0002-7863
ISSN (online)	1520-5126
ISSN	0002-7863
DOI	10.1021%2Fja405725f
Database	NAL-Catalogue (AGRICOLA)

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