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  1. Article ; Online: Towards characterized convalescent plasma for COVID-19

    Hans P. Verkerke / Cheryl L. Maier

    EClinicalMedicine, Vol 26, Iss , Pp 100545- (2020)

    The dose matters

    2020  

    Keywords Medicine (General) ; R5-920 ; covid19
    Language English
    Publishing date 2020-09-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: A flow-cytometry-based protocol using diverse cell types for detecting autoantibodies from human plasma and serum samples

    Andrew Kam Ho Wong / Deanna A. Kulpa / Guido Silvestri / Cheryl L. Maier

    STAR Protocols, Vol 2, Iss 4, Pp 100924- (2021)

    2021  

    Abstract: Summary: Here, we describe a protocol for cell-based detection of autoantibodies from human plasma and serum samples using a standard flow cytometer. The protocol allows detection of autoantibodies against a wide array of extracellular antigens. Antigen ... ...

    Abstract Summary: Here, we describe a protocol for cell-based detection of autoantibodies from human plasma and serum samples using a standard flow cytometer. The protocol allows detection of autoantibodies against a wide array of extracellular antigens. Antigen coverage is limited to the cell types tested, and researchers will need to further determine if autoantibody-positive samples correlate with cytotoxic or clinical outcomes. This protocol is less expensive and faster to perform when compared to protein microarrays and requires no prior knowledge of potential targets.For complete details on the use and execution of this protocol, please refer to Wong et al. (2021).
    Keywords Antibody ; Cell Biology ; Cell-based Assays ; Flow Cytometry/Mass Cytometry ; Health Sciences ; Science (General) ; Q1-390
    Language English
    Publishing date 2021-12-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Broad auto-reactive IgM responses are common in critically ill patients, including those with COVID-19

    Andrew Kam Ho Wong / Isaac Woodhouse / Frank Schneider / Deanna A. Kulpa / Guido Silvestri / Cheryl L. Maier

    Cell Reports Medicine, Vol 2, Iss 6, Pp 100321- (2021)

    2021  

    Abstract: Summary: The pathogenesis of severe coronavirus disease 2019 (COVID-19) remains poorly understood. While several studies suggest that immune dysregulation plays a central role, the key mediators of this process are yet to be defined. Here, we demonstrate ...

    Abstract Summary: The pathogenesis of severe coronavirus disease 2019 (COVID-19) remains poorly understood. While several studies suggest that immune dysregulation plays a central role, the key mediators of this process are yet to be defined. Here, we demonstrate that plasma from a high proportion (93%) of critically ill COVID-19 patients, but not healthy controls, contains broadly auto-reactive immunoglobulin M (IgM) and less frequently auto-reactive IgG or IgA. Importantly, these auto-IgMs preferentially recognize primary human lung cells in vitro, including pulmonary endothelial and epithelial cells. By using a combination of flow cytometry, analytical proteome microarray technology, and lactose dehydrogenase (LDH)-release cytotoxicity assays, we identify high-affinity, complement-fixing, auto-reactive IgM directed against 260 candidate autoantigens, including numerous molecules preferentially expressed on the cellular membranes of pulmonary, vascular, gastrointestinal, and renal tissues. These findings suggest that broad IgM-mediated autoimmune reactivity may be involved in the pathogenesis of severe COVID-19, thereby identifying a potential target for therapeutic interventions.
    Keywords Medicine (General) ; R5-920
    Subject code 610
    Language English
    Publishing date 2021-06-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Estimating severe acute respiratory coronavirus virus 2 (SARS-CoV-2) seroprevalence from residual clinical blood samples, January–March 2021

    Daniel S. Graciaa / Hans P. Verkerke / Jeannette Guarner / Ana Maria Moldoveanu / Narayanaiah Cheedarla / Connie M. Arthur / Cheryl L. Maier / Andrew Neish / Sara C. Auld / Angie Campbell / Neel R. Gandhi / John D. Roback / N. Sarita Shah

    Antimicrobial Stewardship & Healthcare Epidemiology, Vol

    2022  Volume 2

    Abstract: We describe severe acute respiratory coronavirus virus 2 (SARS-CoV-2) IgG seroprevalence and antigenemia among patients at a medical center in January–March 2021 using residual clinical blood samples. The overall seroprevalences were 17% by infection and ...

    Abstract We describe severe acute respiratory coronavirus virus 2 (SARS-CoV-2) IgG seroprevalence and antigenemia among patients at a medical center in January–March 2021 using residual clinical blood samples. The overall seroprevalences were 17% by infection and 16% by vaccination. Spent or residual samples are a feasible alternative for rapidly estimating seroprevalence or monitoring trends in infection and vaccination.
    Keywords Infectious and parasitic diseases ; RC109-216 ; Public aspects of medicine ; RA1-1270
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher Cambridge University Press
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Validation of an admission coagulation panel for risk stratification of COVID-19 patients.

    Darwish Alabyad / Srikant Rangaraju / Michael Liu / Rajeel Imran / Christine L Kempton / Milad Sharifpour / Sara C Auld / Manila Gaddh / Roman Sniecinski / Cheryl L Maier / Jeannette Guarner / Alexander Duncan / Fadi Nahab

    PLoS ONE, Vol 16, Iss 3, p e

    2021  Volume 0248230

    Abstract: Background There is limited data on the markers of coagulation and hemostatic activation (MOCHA) profile in Coronavirus disease 2019 (COVID-19) and its ability to identify COVID-19 patients at risk for thrombotic events and other complications. Methods ... ...

    Abstract Background There is limited data on the markers of coagulation and hemostatic activation (MOCHA) profile in Coronavirus disease 2019 (COVID-19) and its ability to identify COVID-19 patients at risk for thrombotic events and other complications. Methods Hospitalized patients with confirmed SARS-COV-2 from four Atlanta hospitals were included in this observational cohort study and underwent admission testing of MOCHA parameters (plasma d-dimer, prothrombin fragment 1.2, thrombin-antithrombin complex, fibrin monomer). Clinical outcomes included deep vein thrombosis, pulmonary embolism, myocardial infarction, ischemic stroke, access line thrombosis, ICU admission, intubation and mortality. Main results Of 276 patients (mean age 59 ± 6.4 years, 47% female, 62% African American), 45 (16%) had a thrombotic endpoint. Each MOCHA parameter was independently associated with a thrombotic event (p<0.05) and ≥ 2 abnormalities was associated with thrombotic endpoints (OR 3.3, 95% CI 1.2-8.8) as were admission D-dimer ≥ 2000 ng/mL (OR 3.1, 95% CI 1.5-6.6) and ≥ 3000 ng/mL (OR 3.6, 95% CI 1.6-7.9). However, only ≥ 2 MOCHA abnormalities were associated with ICU admission (OR 3.0, 95% CI 1.7-5.2) and intubation (OR 3.2, 95% CI 1.6-6.4). MOCHA and D-dimer cutoffs were not associated with mortality. MOCHA with <2 abnormalities (26% of the cohort) had 89% sensitivity and 93% negative predictive value for a thrombotic endpoint. Conclusions An admission MOCHA profile is useful to risk-stratify COVID-19 patients for thrombotic complications and more effective than isolated d-dimer for predicting risk of ICU admission and intubation.
    Keywords Medicine ; R ; Science ; Q
    Subject code 610
    Language English
    Publishing date 2021-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Multiplatform analyses reveal distinct drivers of systemic pathogenesis in adult versus pediatric severe acute COVID-19

    Samuel Druzak / Elizabeth Iffrig / Blaine R. Roberts / Tiantian Zhang / Kirby S. Fibben / Yumiko Sakurai / Hans P. Verkerke / Christina A. Rostad / Ann Chahroudi / Frank Schneider / Andrew Kam Ho Wong / Anne M. Roberts / Joshua D. Chandler / Susan O. Kim / Mario Mosunjac / Marina Mosunjac / Rachel Geller / Igor Albizua / Sean R. Stowell /
    Connie M. Arthur / Evan J. Anderson / Anna A. Ivanova / Jun Ahn / Xueyun Liu / Kristal Maner-Smith / Thomas Bowen / Mirko Paiardini / Steve E. Bosinger / John D. Roback / Deanna A. Kulpa / Guido Silvestri / Wilbur A. Lam / Eric A. Ortlund / Cheryl L. Maier

    Nature Communications, Vol 14, Iss 1, Pp 1-

    2023  Volume 22

    Abstract: In this work, authors take a multiomics and microfluidics-based approach to elucidate the mechanism of endothelial damage in critical illness associated with SARS-CoV-2. ...

    Abstract In this work, authors take a multiomics and microfluidics-based approach to elucidate the mechanism of endothelial damage in critical illness associated with SARS-CoV-2.
    Keywords Science ; Q
    Language English
    Publishing date 2023-04-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Transendothelial migration enables subsequent transmigration of neutrophils through underlying pericytes.

    Chantal E Ayres-Sander / Holly Lauridsen / Cheryl L Maier / Parid Sava / Jordan S Pober / Anjelica L Gonzalez

    PLoS ONE, Vol 8, Iss 3, p e

    2013  Volume 60025

    Abstract: During acute inflammation, neutrophil recruitment into extravascular tissue requires neutrophil tethering and rolling on cytokine-activated endothelial cells (ECs), tight adhesion, crawling towards EC junctions and transendothelial migration (TEM). ... ...

    Abstract During acute inflammation, neutrophil recruitment into extravascular tissue requires neutrophil tethering and rolling on cytokine-activated endothelial cells (ECs), tight adhesion, crawling towards EC junctions and transendothelial migration (TEM). Following TEM, neutrophils must still traverse the subendothelial basement membrane and network of pericytes (PCs). Until recently, the contribution of the PC layer to neutrophil recruitment was largely ignored. Here we analyze human neutrophil interactions with interleukin (IL)-1β-activated human EC monolayers, PC monolayers and EC/PC bilayers in vitro. Compared to EC, PC support much lower levels of neutrophil binding (54.6% vs. 7.1%, respectively) and transmigration (63.7 vs. 8.8%, respectively) despite comparable levels of IL-8 (CXCL8) synthesis and display. Remarkably, EC/PC bilayers support intermediate levels of transmigration (37.7%). Neutrophil adhesion to both cell types is Mac-1-dependent and while ICAM-1 transduction of PCs increases neutrophil adhesion to (41.4%), it does not increase transmigration through PC monolayers. TEM, which increases neutrophil Mac-1 surface expression, concomitantly increases the ability of neutrophils to traverse PCs (19.2%). These data indicate that contributions from both PCs and ECs must be considered in evaluation of microvasculature function in acute inflammation.
    Keywords Medicine ; R ; Science ; Q
    Subject code 571
    Language English
    Publishing date 2013-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Initiation and Regulation of Complement during Hemolytic Transfusion Reactions

    Sean R. Stowell / Anne M. Winkler / Cheryl L. Maier / C. Maridith Arthur / Nicole H. Smith / Kathryn R. Girard-Pierce / Richard D. Cummings / James C. Zimring / Jeanne E. Hendrickson

    Clinical and Developmental Immunology, Vol

    2012  Volume 2012

    Abstract: Hemolytic transfusion reactions represent one of the most common causes of transfusion-related mortality. Although many factors influence hemolytic transfusion reactions, complement activation represents one of the most common features associated with ... ...

    Abstract Hemolytic transfusion reactions represent one of the most common causes of transfusion-related mortality. Although many factors influence hemolytic transfusion reactions, complement activation represents one of the most common features associated with fatality. In this paper we will focus on the role of complement in initiating and regulating hemolytic transfusion reactions and will discuss potential strategies aimed at mitigating or favorably modulating complement during incompatible red blood cell transfusions.
    Keywords Medicine ; R ; Internal medicine ; RC31-1245 ; Specialties of internal medicine ; RC581-951 ; Immunologic diseases. Allergy ; RC581-607
    Language English
    Publishing date 2012-01-01T00:00:00Z
    Publisher Hindawi Publishing Corporation
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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