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Article ; Online: An Alternative Cell Therapy for Cancers

Li-Jie Hsu / Chao-Lin Liu / Ming-Ling Kuo / Chia-Ning Shen / Chia-Rui Shen

Biomedicines, Vol 9, Iss 1323, p

Induced Pluripotent Stem Cell (iPSC)-Derived Natural Killer Cells

2021 Volume 1323

Abstract: Cell therapy is usually defined as the treatment or prevention of human disease by supplementation with cells that have been selected, manipulated, and pharmacologically treated or altered outside the body (ex vivo). Induced pluripotent stem cells (iPSCs) ...

Abstract	Cell therapy is usually defined as the treatment or prevention of human disease by supplementation with cells that have been selected, manipulated, and pharmacologically treated or altered outside the body (ex vivo). Induced pluripotent stem cells (iPSCs), with their unique characteristics of indefinite expansion in cultures and genetic modifications, represent an ideal cell source for differentiation into specialized cell types. Cell therapy has recently become one of the most promising therapeutic approaches for cancers, and different immune cell types are selected as therapeutic platforms. Natural killer (NK) cells are shown to be effective tumor cell killers and do not cause graft-vs-host disease (GVHD), making them excellent candidates for, and facilitating the development of, “off-the-shelf” cell therapies. In this review, we summarize the progress in the past decade in the advent of iPSC technology and review recent developments in gene-modified iPSC-NK cells as readily available “off-the-shelf” cellular therapies.
Keywords	induced pluripotent stem cells (iPSCs) ; natural killer cells (NK cells) ; cell therapy ; Biology (General) ; QH301-705.5
Subject code	610
Language	English
Publishing date	2021-09-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Transparent tissue in solid state for solvent-free and antifade 3D imaging

Fu-Ting Hsiao / Hung-Jen Chien / Ya-Hsien Chou / Shih-Jung Peng / Mei-Hsin Chung / Tzu-Hui Huang / Li-Wen Lo / Chia-Ning Shen / Hsiu-Pi Chang / Chih-Yuan Lee / Chien-Chia Chen / Yung-Ming Jeng / Yu-Wen Tien / Shiue-Cheng Tang

Nature Communications, Vol 14, Iss 1, Pp 1-

2023 Volume 12

Abstract: Abstract Optical clearing with high-refractive-index (high-n) reagents is essential for 3D tissue imaging. However, the current liquid-based clearing condition and dye environment suffer from solvent evaporation and photobleaching, causing difficulties ... ...

Abstract	Abstract Optical clearing with high-refractive-index (high-n) reagents is essential for 3D tissue imaging. However, the current liquid-based clearing condition and dye environment suffer from solvent evaporation and photobleaching, causing difficulties in maintaining the tissue optical and fluorescent features. Here, using the Gladstone-Dale equation [(n−1)/density=constant] as a design concept, we develop a solid (solvent-free) high-n acrylamide-based copolymer to embed mouse and human tissues for clearing and imaging. In the solid state, the fluorescent dye-labeled tissue matrices are filled and packed with the high-n copolymer, minimizing scattering in in-depth imaging and dye fading. This transparent, liquid-free condition provides a friendly tissue and cellular environment to facilitate high/super-resolution 3D imaging, preservation, transfer, and sharing among laboratories to investigate the morphologies of interest in experimental and clinical conditions.
Keywords	Science ; Q
Language	English
Publishing date	2023-06-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Generation of three induced pluripotent stem cell lines from type 2 diabetic patients with ocular complications

Edward Po-Fan Chu / Candy Hsin-Hua Cho / Wen-Jane Lee / I-Te Lee / I-Fen Cheng / Tzu-Chien Kuo / Ruei-Ying Chen / Wayne Huey-Herng Sheu / Chia-Ning Shen

Stem Cell Research, Vol 49, Iss , Pp 102109- (2020)

2020

Abstract: Retinopathy is a well-known ocular complication that occurs in patients with type 2 diabetes (T2D). Recent evidence also indicates that diabetic patients have an increased prevalence of dry eye syndrome. However, the etiologies of both diabetic ... ...

Abstract	Retinopathy is a well-known ocular complication that occurs in patients with type 2 diabetes (T2D). Recent evidence also indicates that diabetic patients have an increased prevalence of dry eye syndrome. However, the etiologies of both diabetic retinopathy (DR) and dry eye disease are complex, and their associations with T2D remains to be fully understood. Patient-derived human induced pluripotent stem cells (hiPSCs) enable the generation of disease-specific retinal tissues such as retinal pigment epithelium and lacrimal gland to model disease pathogenesis. Here, we describe the establishment of three hiPSC lines from T2D patients with PDR or dry eye disease.
Keywords	Biology (General) ; QH301-705.5
Language	English
Publishing date	2020-12-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Correction

Amani Al-Adsani / Zoë D Burke / Daniel Eberhard / Katherine L Lawrence / Chia-Ning Shen / Anil K Rustgi / Hiroshi Sakaue / J Mark Farrant / David Tosh

PLoS ONE, Vol 14, Iss 7, p e

Dexamethasone Treatment Induces the Reprogramming of Pancreatic Acinar Cells to Hepatocytes and Ductal Cells.

2019 Volume 0219419

Abstract: This corrects the article DOI:10.1371/journal.pone.0013650.]. ...

Abstract	[This corrects the article DOI:10.1371/journal.pone.0013650.].
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2019-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Establishment of three human induced pluripotent stem cell lines from a type 1 diabetic family harboring sequence variants associated with autoimmunity

Edward Po-Fan Chu / Chia-Hung Lin / Candy Hsin-Hua Cho / I-Fen Cheng / Tzu-Chien Kuo / Ruei-Ying Chen / Chia-Nan Liao / Jen-Chien Cheng / Jason Isheng Tsai / Po-Chuan Wang / Shing-Jyh Chang / Chia-Ning Shen

Stem Cell Research, Vol 49, Iss , Pp 102029- (2020)

2020

Abstract: Type 1 diabetes (T1D) is characterized by the autoimmune destruction of insulin-producing β cells. Genetic studies have identified > 60 T1D risk loci that harbor genes with disease-causative alleles. However, determining the biological effects of such ... ...

Abstract	Type 1 diabetes (T1D) is characterized by the autoimmune destruction of insulin-producing β cells. Genetic studies have identified > 60 T1D risk loci that harbor genes with disease-causative alleles. However, determining the biological effects of such loci is often difficult due to limited tissue availability. Disease-specific human induced pluripotent stem cells (hiPSCs) are a valuable resource for modeling T1D pathogenesis. In particular, families with complete disease penetrance offer an opportunity to further dissect T1D risk loci. Here, we describe the generation of three hiPSC lines from a T1D family with sequence variants associated with autoimmunity.
Keywords	Biology (General) ; QH301-705.5
Language	English
Publishing date	2020-12-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Lumenal Galectin-9-Lamp2 interaction regulates lysosome and autophagy to prevent pathogenesis in the intestine and pancreas

Janaki N. Sudhakar / Hsueh-Han Lu / Hung-Yu Chiang / Ching-Shu Suen / Ming-Jing Hwang / Sung-Yu Wu / Chia-Ning Shen / Yao-Ming Chang / Fu-An Li / Fu-Tong Liu / Jr-Wen Shui

Nature Communications, Vol 11, Iss 1, Pp 1-

2020 Volume 17

Abstract: Galectins are carbohydrate binding proteins previously implicated in sensing and repairing damaged lysosomes. Here, the authors show that galectin-9 has specific lysosomal roles in autophagy and contributes to cell degeneration and apoptosis in colitis ... ...

Abstract	Galectins are carbohydrate binding proteins previously implicated in sensing and repairing damaged lysosomes. Here, the authors show that galectin-9 has specific lysosomal roles in autophagy and contributes to cell degeneration and apoptosis in colitis and pancreatitis in mice.
Keywords	Science ; Q
Language	English
Publishing date	2020-08-01T00:00:00Z
Publisher	Nature Publishing Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Ex Vivo Expansion and Drug Sensitivity Profiling of Circulating Tumor Cells from Patients with Small Cell Lung Cancer

Hsin-Lun Lee / Jeng-Fong Chiou / Peng-Yuan Wang / Long-Sheng Lu / Chia-Ning Shen / Han-Lin Hsu / Thierry Burnouf / Lai-Lei Ting / Pai-Chien Chou / Chi-Li Chung / Kai-Ling Lee / Her-Shyong Shiah / Yen-Lin Liu / Yin-Ju Chen

Cancers, Vol 12, Iss 3394, p

2020 Volume 3394

Abstract: Small cell lung cancer (SCLC) represents one of the most aggressive malignancies among cancer types. Not only tumor sample availability is limited, but also the ability for tumor cells to rapidly acquire drug resistance are the rate-limiting bottlenecks ... ...

Abstract	Small cell lung cancer (SCLC) represents one of the most aggressive malignancies among cancer types. Not only tumor sample availability is limited, but also the ability for tumor cells to rapidly acquire drug resistance are the rate-limiting bottlenecks for overall survival in current clinical settings. A liquid biopsy capable of capturing and enriching circulating tumor cells (CTCs), together with the possibility of drug screening, is a promising solution. Here, we illustrate the development of a highly efficient ex vivo CTC expansion system based on binary colloidal crystals substrate. Clinical samples were enrolled from 22 patients with SCLC in the study. The CTCs were enriched and expanded from the collected peripheral blood samples. Expanded cells were analyzed for protein expression and observed for drug sensitivity with the use of immunofluorescence and ATP titer evaluation, respectively. Successful CTC spheroid proliferation was established after 4 weeks within 82% of all the collected peripheral blood samples from enrolled patients. Upon immunofluorescence analysis, the enriched cells showed positive markers for EpCAM, TTF-1, synaptophysin and negative for CD45. Additionally, the expanded CTCs demonstrated marked heterogeneity in the expression of E-cadherin and N-cadherin. In a preliminary case series, the drug sensitivity of patient-derived CTC to cisplatin and etoposide was studied to see the correlation with the corresponding therapeutic outcome. In conclusion, our study demonstrates that it is possible to efficiently expand CTCs from SCLC within a clinically relevant time frame; the biomarker information generated from enriched CTCs can assist the selection of effective drugs and improve disease outcome.
Keywords	small cell lung cancer ; circulating tumor cells ; primary cell culture ; liquid biopsy ; personalized medicine ; Neoplasms. Tumors. Oncology. Including cancer and carcinogens ; RC254-282
Subject code	610
Language	English
Publishing date	2020-11-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Lumenal Galectin-9-Lamp2 interaction regulates lysosome and autophagy to prevent pathogenesis in the intestine and pancreas

Janaki N. Sudhakar / Hsueh-Han Lu / Hung-Yu Chiang / Ching-Shu Suen / Ming-Jing Hwang / Sung-Yu Wu / Chia-Ning Shen / Yao-Ming Chang / Fu-An Li / Fu-Tong Liu / Jr-Wen Shui

Nature Communications, Vol 11, Iss 1, Pp 1-

2020 Volume 17

Abstract	Galectins are carbohydrate binding proteins previously implicated in sensing and repairing damaged lysosomes. Here, the authors show that galectin-9 has specific lysosomal roles in autophagy and contributes to cell degeneration and apoptosis in colitis and pancreatitis in mice.
Keywords	Science ; Q
Language	English
Publishing date	2020-08-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: All-trans retinoic acid ameliorates glycemic control in diabetic mice via modulating pancreatic islet production of vascular endothelial growth factor-A

Chien, Chiao-Yun / Tze-An Yuan / Candy Hsin-Hua Cho / Fang-Pei Chang / Wan-Yu Mao / Ruei-Ren Wu / Hsuan-Shu Lee / Chia-Ning Shen

Biochemical and biophysical research communications. 2016,

2016

Abstract: Patients with type 1 diabetes mellitus are associated with impairment in vitamin A metabolism. This study evaluated whether treatment with retinoic acid, the biologically active metabolite of vitamin A, can ameliorate diabetes. All-trans retinoic acid ( ... ...

Abstract	Patients with type 1 diabetes mellitus are associated with impairment in vitamin A metabolism. This study evaluated whether treatment with retinoic acid, the biologically active metabolite of vitamin A, can ameliorate diabetes. All-trans retinoic acid (atRA) was used to treat streptozotocin (STZ)-induced diabetic mice which revealed atRA administration ameliorated blood glucose levels of diabetic mice. This hyperglycemic amelioration was accompanied by an increase in the amount of Î² cells co-expressed Pdx1 and insulin and by restoration of the vascular laminin expression. The atRA-induced production of vascular endothelial growth factor-A from the pancreatic islets was possibly the key factor that mediated the restoration of islet vascularity and recovery of Î²âcell mass. Furthermore, the combination of islet transplantation and atRA administration significantly rescued hyperglycemia in diabetic mice. These findings suggest that vitamin A derivatives can potentially be used as a supplementary treatment to improve diabetes management and glycemic control.
Keywords	animal disease models ; blood glucose ; disease control ; glycemic control ; hyperglycemia ; insulin ; insulin-dependent diabetes mellitus ; islets of Langerhans ; laminin ; metabolites ; mice ; patients ; retinoic acid ; streptozotocin ; vascular endothelial growth factor A ; vitamin A ; vitamin metabolism ; All-trans retinoic acid ; Vascular endothelial growth factor ; Islet vascularization ; Type 1 diabetes mellitus ; AtRA ; STZ ; T1DM ; VEGF-A ; RAR
Language	English
Size	p. .
Publishing place	Elsevier Inc.
Document type	Article
Note	Pre-press version
ZDB-ID	205723-2
ISSN	0006-291X ; 0006-291X
ISSN (online)	0006-291X
ISSN	0006-291X
DOI	10.1016/j.bbrc.2016.06.151
Database	NAL-Catalogue (AGRICOLA)

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Article: Maternal vitamin A deficiency during pregnancy affects vascularized islet development

Chien, Chiao-Yun / Candy Hsin-Hua Cho / Chia-Ning Shen / David Tosh / Hsuan-Shu Lee / Kuo-I Lin / Ruei-Ren Wu / Wan-Yu Mao

Journal of nutritional biochemistry. 2016 Oct., v. 36

2016

Abstract: Vitamin A deficiency is known to affect 20 million pregnant women worldwide. However, the prenatal effects of maternal vitamin A deficiency on pancreas development have not been clearly determined. The present study examined how maternal vitamin A ... ...

Abstract	Vitamin A deficiency is known to affect 20 million pregnant women worldwide. However, the prenatal effects of maternal vitamin A deficiency on pancreas development have not been clearly determined. The present study examined how maternal vitamin A deficiency affects fetal islet development. Vitamin A-deficient mice were generated by feeding female mice with a chemically defined diet lacking vitamin A prior to mating as well as during pregnancy. We found that maternal vitamin A deficiency during pregnancy affected fetal pancreas development. Although the exocrine differentiation appeared normal, development of islet tissue was impaired. In the pancreas of neonatal mice, only a few endocrine cell clusters were formed, and these cell clusters lacked capillary endothelial cells. To further determine how vitamin A metabolites, such as retinoic acid, regulate vascularized islet development, ex vivo culture of embryonic pancreas either in the presence of 4-diethylaminobenzaldehyde (DEAB; an inhibitor of retinaldehyde dehydrogenase), all-trans retinoic acid (atRA) or retinoic acid receptor agonist (E)-4-[2-(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthylenyl)-1-propenyl] benzoic acid (TTNPB) was carried out. We found that the addition of DEAB blocked vascularization and suppressed β-cell differentiation. Conversely, atRA or TTNPB promoted β-cell differentiation accompanied by enhanced expression of vascular basement component, laminin. We further demonstrated that atRA regulated vascularization via upregulating vascular endothelial growth factor-A (VEGF-A) secretion in embryonic pancreas and treatment with VEGF-A was able to partially rescue vascularization and β-cell differentiation in DEAB-treated embryonic pancreas cultures. The findings explain why maternal vitamin A deficiency affects fetal islet development and support an essential role of retinoid signaling in regulating vascularized islet development.
Keywords	agonists ; diet ; endothelial cells ; females ; islets of Langerhans ; laminin ; maternal effect ; metabolites ; mice ; neonates ; pregnancy ; retinoic acid ; secretion ; vascular endothelial growth factor A ; vitamin A ; vitamin A deficiency
Language	English
Dates of publication	2016-10
Size	p. 51-59.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	1014929-6
ISSN	1873-4847 ; 0955-2863
ISSN (online)	1873-4847
ISSN	0955-2863
DOI	10.1016/j.jnutbio.2016.07.010
Database	NAL-Catalogue (AGRICOLA)

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