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Article ; Online: Effect of early oseltamivir on outpatients without hypoxia with suspected COVID-19.

Chiba, Satoru

2020 Volume 133, Issue 7-8, Page(s) 292–297

Abstract: Background: Since December 2019 coronavirus disease 2019 (COVID-19) outbreaks have occurred in China and many countries around the world. The effectiveness of oseltamivir on COVID-19 suspected outpatients without hypoxia was evaluated.: Methods: A ... ...

Abstract	Background: Since December 2019 coronavirus disease 2019 (COVID-19) outbreaks have occurred in China and many countries around the world. The effectiveness of oseltamivir on COVID-19 suspected outpatients without hypoxia was evaluated. Methods: A total of 16 COVID-19 suspected medical personnel and their cohabitation families without hypoxia, who came to the adult fever clinic from March to June 2020 were studied. All patients received antiviral therapy (oseltamivir) and antibacterial therapy together. Results: Most of the infected patients were female (10, 63%), 1 had diabetes (6%) and 3 hypertension (19%). The median age was 45 years (interquartile range [IQR] 31-60 years). Oseltamivir administration made the temperature fall within 24 h in part (10, 63%). Clinical data were compared between patients receiving early treatment (ET) with oseltamivir, initiated within 24 h, and patients administered late treatment (LT), initiated after this time point. Duration of fever was shorter in the ET group than in the LT group (31 ± 21 h versus 94 ± 38 h; p < 0.001). The time from fever onset to treatment initiation correlated with duration of fever (r = 0.77; p < 0.001) and the time from peak to decline (r = 0.58; p < 0.05). Conclusion: These findings suggest that early oseltamivir administration may lower the duration of fever in COVID-19 suspected outpatients without hypoxia when it is used in combination with antibacterial therapy.
MeSH term(s)	Adult ; Antiviral Agents/adverse effects ; COVID-19 ; China/epidemiology ; Female ; Humans ; Hypoxia/diagnosis ; Hypoxia/drug therapy ; Influenza, Human/diagnosis ; Influenza, Human/drug therapy ; Influenza, Human/epidemiology ; Male ; Middle Aged ; Oseltamivir/therapeutic use ; Outpatients ; SARS-CoV-2
Chemical Substances	Antiviral Agents ; Oseltamivir (20O93L6F9H)
Language	English
Publishing date	2020-12-09
Publishing country	Austria
Document type	Journal Article
ZDB-ID	200462-8
ISSN	1613-7671 ; 0043-5325 ; 0300-5178
ISSN (online)	1613-7671
ISSN	0043-5325 ; 0300-5178
DOI	10.1007/s00508-020-01780-0
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Article ; Online: Keigai-rengyo-to as post-exposure prophylaxis for severe acute respiratory syndrome coronavirus 2 infection.

Chiba, Satoru / Shinohara, Kaoru

Respiratory investigation

2023 Volume 61, Issue 6, Page(s) 669–674

Abstract: Background: Effective prevention against COVID-19 is urgently required to control vaccine breakthrough infection. Laboratory and clinical data suggested that Keigai-rengyo-to (KRT) performs biological activity against severe acute respiratory syndrome ... ...

Abstract	Background: Effective prevention against COVID-19 is urgently required to control vaccine breakthrough infection. Laboratory and clinical data suggested that Keigai-rengyo-to (KRT) performs biological activity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We investigated whether KRT could prevent SARS-CoV-2 in medical personnel exposed to patients with COVID-19. Methods: We conducted an open-label controlled clinical trial of medical personnel after COVID-19 vaccination at our hospital (ClinicalTrials.gov: UMIN000048389). Participants were close contacts recently exposed (<72 h) to patients with COVID-19. We provided the participants with KRT (7.5 g/day for 5 days) or no drug as a control. The primary endpoint was nicking endonuclease amplification reaction or polymerase chain reaction confirming incident SARS-CoV-2 infection. Safety was assessed in all treated participants. Results: Between January and September 2022, 38 close contacts were assigned: 20 to the KRT group and 18 to the control group. During 2 weeks of follow-up, 10/38 (26%) participants had new-onset COVID-19. The incidence of COVID-19 was significantly lower in the KRT group (2/20; 10%) than in the control group (8/18; 44%), with a medium effect size (p < 0.05; phi coefficient = -0.391; total absolute risk reduction: 34.4% points). The number needed to treat to prevent the occurrence of a COVID-19 case was 2.9. The overall relative risk was 0.23 (95% confidence interval: 0.06-0.78). No serious safety problems were detected. Conclusion: Post-exposure prophylaxis with KRT can prevent the onset of COVID-19 in close contacts after vaccination. More randomized clinical trials with larger samples are required to better evaluate KRT as a post-exposure prophylaxis of SARS-CoV-2.
MeSH term(s)	Humans ; COVID-19/prevention & control ; COVID-19 Vaccines ; Post-Exposure Prophylaxis ; SARS-CoV-2
Chemical Substances	COVID-19 Vaccines ; keigai-rengyo-to
Language	English
Publishing date	2023-09-12
Publishing country	Netherlands
Document type	Controlled Clinical Trial ; Journal Article
ZDB-ID	2660821-2
ISSN	2212-5353 ; 2212-5345
ISSN (online)	2212-5353
ISSN	2212-5345
DOI	10.1016/j.resinv.2023.07.004
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Article ; Online: Restoration of the Functional nif Gene Cluster by Complex Recombination Events during Heterocyst Development in the Nitrogen-Fixing Cyanobacterium Calothrix sp. NIES-4101.

Uesaka, Kazuma / Banba, Mari / Chiba, Sotaro / Fujita, Yuichi

Plant & cell physiology

2024

Abstract: In the genome of the heterocystous cyanobacterium Calothrix sp. NIES-4101 (NIES-4101), the four genes essential for nitrogen fixation (nifB, nifH, nifD, and nifK) are highly fragmented into 13 parts in a 350-kb chromosomal region, and four of these parts ...

Abstract	In the genome of the heterocystous cyanobacterium Calothrix sp. NIES-4101 (NIES-4101), the four genes essential for nitrogen fixation (nifB, nifH, nifD, and nifK) are highly fragmented into 13 parts in a 350-kb chromosomal region, and four of these parts are encoded in the reverse strand. Such a complex fragmentation feature makes it difficult to restore the intact nifBHDK genes by the excision mechanism found in the nifD gene of the Anabaena sp. PCC 7120 heterocyst. To examine the nitrogen-fixing ability of NIES-4101, we confirmed that NIES-4101 grew well on combined nitrogen-free medium and showed high nitrogenase activity, which strongly suggested that the complete nifBHDK genes are restored by a complex recombination process in heterocysts. Next, we resequenced the genome prepared from cells grown under nitrogen-fixing conditions. Two contigs covering the complete nifHDK and nifB genes were found by de novo assembly of the sequencing reads. In addition, DNA fragments covering the nifBHDK operon were successfully amplified by PCR. We propose that the process of nifBHDK restoration occurs as follows. First, the nifD-nifK genes are restored by four excision events. Then the complete nifH and nifB genes are restored by two excision events followed by two successive inversion events between the inverted repeat sequences and one excision event, forming the functional nif gene cluster, nifB-fdxN-nifS-nifU-nifH-nifD-nifK. All genes coding recombinases responsible for these nine recombination events are located close to the terminal repeat sequences. The restoration of the nifBHDK genes in NIES-4101 is the most complex genome reorganization reported in heterocystous cyanobacteria.
Language	English
Publishing date	2024-02-02
Publishing country	Japan
Document type	Journal Article
ZDB-ID	208907-5
ISSN	1471-9053 ; 0032-0781
ISSN (online)	1471-9053
ISSN	0032-0781
DOI	10.1093/pcp/pcae011
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Article: [Conflict of interest management in the Japanese Society of Hematology].

Chiba, Shigeru

Rinsho ketsueki] The Japanese journal of clinical hematology

2019 Volume 60, Issue 9, Page(s) 1366–1371

Abstract: A conflict of interest (COI) is a conflict between private interests and one's official responsibilities. COI is unavoidable and thus needs to be managed. Debates about COI in medical research area began in the United States in the 1980s. COI clause was ... ...

Abstract	A conflict of interest (COI) is a conflict between private interests and one's official responsibilities. COI is unavoidable and thus needs to be managed. Debates about COI in medical research area began in the United States in the 1980s. COI clause was added to the Helsinki Declaration in the year of 2000 after the Gelsinger affair. Discussions in Japan subsequently followed, and COI management guidelines have been sequentially presented by different organizations, particularly after the Diovan incident in 2013. Guidelines presented by the Japan Medical Association (JMA) in 2017 are currently regarded as standard. COI disclosure is required at research presentations and medical practice guideline announcements, among others, and should be carefully managed in the latter. In 2018, the Japanese Society of Hematology announced revision of the Japanese Society of Internal Medicine common guidance, presenting it in accordance with the JMA's guidelines. In the "Specific Clinical Research" section defined in the "Clinical Research Method Enforcement Rule", people who should not take a representative position or who should pay special attention when taking it are defined.
MeSH term(s)	Conflict of Interest ; Disclosure ; Hematology/standards ; Japan ; Societies, Medical/standards
Language	Japanese
Publishing date	2019-10-09
Publishing country	Japan
Document type	Journal Article
ZDB-ID	390900-1
ISSN	0485-1439
ISSN	0485-1439
DOI	10.11406/rinketsu.60.1366
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Article ; Online: Clarification of Psychiatric Nurses' Intentions and Analysis Contents in Observing Schizophrenia Patient.

Chiba, Shinichi / Tomotake, Masahito / Tsutsumi, Rie

The journal of medical investigation : JMI

2024 Volume 71, Issue 1.2, Page(s) 54–61

Abstract: The purpose of this study was to clarify what psychiatric nurses intended to observe when observing schizophrenia patient and what they analyzed from their observations. Twenty-one experienced nurses were included in the study. Data were collected ... ...

Abstract	The purpose of this study was to clarify what psychiatric nurses intended to observe when observing schizophrenia patient and what they analyzed from their observations. Twenty-one experienced nurses were included in the study. Data were collected through semi-structured interviews, and content analysis was conducted. The results were as follows:Nurses' intentions in observing patient were [Observation of psychiatric symptoms], [Observation of normality or abnormality], [Possibility of self-harm or harming others], [Side effects of antipsychotics], [Degree of communication disorder], [Degree of self-care], [Observation of nutritional status], [Effects of external stimuli on patient], and [Less importance of observing doctor]. Nurses' analysis contents were [Analysis of psychiatric symptoms], [No risk of self-harm or harming others], [Effects of antipsychotics], [Presence of communication disorder], [Lack of trust in doctor], [Problems in daily life], [Consideration of future nursing intervention], [Assessment of normality or abnormality], [Unhealthy physical state], and [Unnecessary analysis]. The results of this study revealed the nurses' observational process. J. Med. Invest. 71 : 54-61, February, 2024.
MeSH term(s)	Humans ; Schizophrenia ; Psychiatric Nursing ; Female ; Adult ; Male ; Intention ; Middle Aged
Language	English
Publishing date	2024-05-11
Publishing country	Japan
Document type	Journal Article
ZDB-ID	1435233-3
ISSN	1349-6867 ; 1343-1420
ISSN (online)	1349-6867
ISSN	1343-1420
DOI	10.2152/jmi.71.54
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Article ; Online: Collective Synthesis of Highly Oxygenated (Furano)germacranolides Derived from Elephantopus mollis and Elephantopus tomentosus.

Patouret, Rémi / Cham, Ning / Chiba, Shunsuke

Angewandte Chemie (International ed. in English)

2024 Volume 63, Issue 19, Page(s) e202402050

Abstract: Germacranolides, secondary metabolites produced by plants, have garnered academic and industrial interest due to their diverse and complex topology as well as a wide array of pharmacological activities. Molephantin, a highly oxygenated germacranolide ... ...

Abstract	Germacranolides, secondary metabolites produced by plants, have garnered academic and industrial interest due to their diverse and complex topology as well as a wide array of pharmacological activities. Molephantin, a highly oxygenated germacranolide isolated from medicinal plants, Elephantopus mollis and Elephantopus tomentosus, has exhibited antitumor, inflammatory, and leishmanicidal activities. Its chemical structure is based on a highly strained ten-membered macrocyclic backbone with an (E,Z)-dienone moiety, which is fused with an α-methylene-γ-butyrolactone and adorned with four successive stereogenic centers. Herein, we report the first synthesis of molephantin in 12 steps starting from readily available building blocks. The synthesis features the highly diastereoselective intermolecular Barbier allylation of the β,γ-unsaturated aldehyde with optically active 3-bromomethyl-5H-furan-2-one intermediate and ensuing Nozaki-Hiyama-Kishi (NHK) macrocyclization for the construction of the highly oxygenated ten-membered macrocyclic framework. This synthetic route enabled access to another germacranolide congener, tomenphantopin F. Furthermore, cycloisomerization of molephantin into 2-deethoxy-2β-hydroxyphantomolin could be facilitated by irradiation with ultraviolet A light (λ
MeSH term(s)	Oxygen/chemistry ; Asteraceae/chemistry ; Stereoisomerism ; Sesquiterpenes, Germacrane/chemistry ; Sesquiterpenes, Germacrane/chemical synthesis ; Furans/chemistry ; Furans/chemical synthesis ; Molecular Structure
Chemical Substances	Oxygen (S88TT14065) ; germacranolide ; Sesquiterpenes, Germacrane ; Furans
Language	English
Publishing date	2024-04-05
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2011836-3
ISSN	1521-3773 ; 1433-7851
ISSN (online)	1521-3773
ISSN	1433-7851
DOI	10.1002/anie.202402050
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Article: Innovative power generator using dielectric elastomers (creating the foundations of an environmentally sustainable society)

Chiba, S / Waki, M

Sustainable chemistry and pharmacy. 2020 Mar., v. 15

2020

Abstract: Power generation using dielectric elastomer (DE) artificial muscle has attracted attention because it is light-weight, low-cost and high-efficiency. This method generates carbon dioxide-free electric power without exhausting rare earth materials or ... ...

Abstract	Power generation using dielectric elastomer (DE) artificial muscle has attracted attention because it is light-weight, low-cost and high-efficiency. This method generates carbon dioxide-free electric power without exhausting rare earth materials or contributing to global warming, earning it the status of an eco-friendly system.This paper considers the opportunities for a DE generator system, namely using them to create the foundations of a Recycling Energy Society. If these opportunities are to be commercially successful, they will have to leverage the DE's advantages over conventional technologies. In this paper, we discuss two ways to use DEs more practically in applications: 1) point power generation, in which a single DE is used alone, and 2) distributed power generation, in which a large number of DEs are gathered as one cluster and distributed. We will also discuss the current status and future of DE generators.
Keywords	carbon dioxide ; generators (equipment) ; global warming ; green chemistry ; muscles ; power generation ; sustainable communities
Language	English
Dates of publication	2020-03
Publishing place	Elsevier B.V.
Document type	Article
ISSN	2352-5541
DOI	10.1016/j.scp.2019.100205
Database	NAL-Catalogue (AGRICOLA)

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Article: [Biomarkers predicting the efficacy of DNA hypomethylating agents in the treatment of myelodysplastic syndromes and acute myeloid leukemia: TET enzymes].

Chiba, Shigeru

Rinsho ketsueki] The Japanese journal of clinical hematology

2018 Volume 59, Issue 5, Page(s) 594–601

Abstract: Azacitidine (AZA), a hypomethylating agent, is widely used in patients with high-risk (HR) myelodysplastic syndromes (MDS). AZA is also believed to aid in the treatment of acute myeloid leukemia (AML). In randomized clinical trials for MDS, major and ... ...

Abstract	Azacitidine (AZA), a hypomethylating agent, is widely used in patients with high-risk (HR) myelodysplastic syndromes (MDS). AZA is also believed to aid in the treatment of acute myeloid leukemia (AML). In randomized clinical trials for MDS, major and overall response rates for AZA alone were 16-33% and 38-60%, respectively. In the AZA-001 trial, the median overall survival (OS) for the AZA group was >24 months compared to 15 months for the control. However, other clinical trials have reported OS periods of 15-21 months, while population-based studies have reported OS rates much shorter than 20 months. Only a fraction of HR-MDS patients appears to benefit from AZA treatment. Furthermore, despite its low-intensity nature, the risk may outweigh the benefit in a significant proportion of patients; therefore, the indication of AZA should be reconsidered if good biomarkers that predict its efficacy are discovered. The response rate to AZA was moderately higher in MDS patients with TET2 mutations than in those without the mutations. Recently, increasing attention has been paid to a report, according to which, majority of the patients with TP53 mutations showed good response to a higher dose of decitabine. The use of AZA and the biomarkers predicting its efficacy is discussed.
MeSH term(s)	Antimetabolites, Antineoplastic ; Azacitidine ; Biomarkers ; DNA ; DNA Methylation ; Humans ; Leukemia, Myeloid, Acute ; Myelodysplastic Syndromes ; Treatment Outcome
Chemical Substances	Antimetabolites, Antineoplastic ; Biomarkers ; DNA (9007-49-2) ; Azacitidine (M801H13NRU)
Language	Japanese
Publishing date	2018-06-06
Publishing country	Japan
Document type	Journal Article
ZDB-ID	390900-1
ISSN	0485-1439
ISSN	0485-1439
DOI	10.11406/rinketsu.59.594
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Article ; Online: A predictive model to estimate fever after receipt of the second dose of Pfizer-BioNTech coronavirus disease 2019 vaccine: An observational cohort study.

Chiba, Satoru / Shinohara, Kaoru

Health science reports

2022 Volume 5, Issue 4, Page(s) e742

Abstract: Background and aims: Fever after coronavirus disease 2019 (COVID-19) vaccination is generally a mild and benign event, but can cause excessive anxiety in younger adults. This study aimed to find key factors that include allergic diseases or physique ... ...

Abstract	Background and aims: Fever after coronavirus disease 2019 (COVID-19) vaccination is generally a mild and benign event, but can cause excessive anxiety in younger adults. This study aimed to find key factors that include allergic diseases or physique that determine fever after vaccination. Methods: We conducted an observational cohort study in our hospital to assess post-COVID-19 vaccination fever from April to June 2021. A total of 153 medical personnel aged 22-86 years of age were involved in the study to receive two doses, intramuscularly 21 days apart, of the Pfizer-BioNTech COVID-19 vaccine (30 μg per dose). Vaccination records were taken more than 72 h after vaccination. Clinical and laboratory variables (age, sex, allergy history, weight, height, serum hemoglobin concentration, and these derivatives) were examined by multivariable logistic regression analysis using the peak axillary temperature in the 4-day period after the second vaccination as a dependent variable. Results: No serious safety problems were detected. The incidence of a postsecond vaccination fever of 37.3°C or above was 29.4%. Logistic regression analysis found age, history of perennial allergic rhinitis, body surface area, body weight, percent overweight, and serum hemoglobin concentration as independent predictors of postvaccination fever. The characteristics of this individual were incorporated into the numerical model of human thermoregulation. The evaluation of this model had a sensitivity of 66.1% and a specificity of 90.7% in the detection of postvaccination fever. The multiple coefficient of determination ( Conclusion: The COVID-19 vaccine induced higher rates of fever during the 4-day period after the second vaccination. Younger age, part of the allergy history, small and light body, and concentrated blood were associated with postvaccination fever.
Language	English
Publishing date	2022-07-20
Publishing country	United States
Document type	Journal Article
ISSN	2398-8835
ISSN (online)	2398-8835
DOI	10.1002/hsr2.742
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Article ; Online: A Case Report of Respiratory Syncytial Virus-Infected 8p Inverted Duplication Deletion Syndrome with Low Natural Killer Cell Activity.

Tokutake, Haruka / Chiba, Satoko

The Tohoku journal of experimental medicine

2022 Volume 257, Issue 4, Page(s) 347–352

Abstract: The 8p inverted duplication deletion [inv dup del(8p)] is a complex structural rearrangement in chromosome 8. Patients with this chromosomal abnormality exhibit developmental delay, facial dysmorphism, central nervous abnormalities, hypotonia, orthopedic ...

Abstract	The 8p inverted duplication deletion [inv dup del(8p)] is a complex structural rearrangement in chromosome 8. Patients with this chromosomal abnormality exhibit developmental delay, facial dysmorphism, central nervous abnormalities, hypotonia, orthopedic abnormalities, and congenital heart defects. However, cellular immune function in inv dup del(8p) syndrome has never been reported. We present the case of a 1-month-old boy with inv dup del(8p) syndrome who had severe respiratory syncytial (RS) virus bronchiolitis. Natural killer (NK) cells are recruited to airway epithelium in the early phase of RS viral infection. A cluster of defensin genes (DEFs), which are deleted in inv dup del(8p), are located in 8p23.1. Human defensins are involved in antiviral activity through the NK cell-mediated cytotoxic pathway and envelope disruption in the normal immune response. This patient showed lower NK cell activity and α-defensin level compared with healthy controls. These results suggest that decreased NK cell activity can result from DEF haploinsufficiency. In addition to a skeletal deformity with chromosomal abnormality, NK cell-mediated immune deficiency may account for the exacerbation of RS virus bronchiolitis.
MeSH term(s)	Bronchiolitis ; Chromosome Deletion ; Chromosome Disorders/complications ; Chromosome Disorders/genetics ; Chromosome Inversion ; Humans ; Infant ; Killer Cells, Natural ; Male ; Respiratory Syncytial Viruses
Language	English
Publishing date	2022-06-30
Publishing country	Japan
Document type	Case Reports ; Journal Article
ZDB-ID	123477-8
ISSN	1349-3329 ; 0040-8727
ISSN (online)	1349-3329
ISSN	0040-8727
DOI	10.1620/tjem.2022.J052
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