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Article ; Online: Stretch-induced reactive oxygen species contribute to the Frank-Starling mechanism.

Kaihara, Keiko / Kai, Hiroaki / Chiba, Yumiko / Naruse, Keiji / Iribe, Gentaro

2023

Abstract: Myocardial stretch physiologically activates NADPH oxidase 2 (NOX2) to increase reactive oxygen species (ROS) production. Although physiological low-level ROS are known to be important as signalling molecules, the role of stretch-induced ROS in the ... ...

Abstract	Myocardial stretch physiologically activates NADPH oxidase 2 (NOX2) to increase reactive oxygen species (ROS) production. Although physiological low-level ROS are known to be important as signalling molecules, the role of stretch-induced ROS in the intact myocardium remains unclear. To address this, we investigated the effects of stretch-induced ROS on myocardial cellular contractility and calcium transients in C57BL/6J and NOX2
Language	English
Publishing date	2023-04-14
Publishing country	England
Document type	Journal Article
ZDB-ID	3115-x
ISSN	1469-7793 ; 0022-3751
ISSN (online)	1469-7793
ISSN	0022-3751
DOI	10.1113/JP284283
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Article ; Online: [Dapagliflozin, a Sodium-Glucose Co-transporter-2 Inhibitor, Acutely Reduces Energy Expenditure in Brown Adipose Tissue via Neural Signals in Mice].

Chiba, Yumiko / Yamada, Tetsuya / Katagiri, Hideki

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan

2018 Volume 138, Issue 7, Page(s) 945–954

Abstract: Selective sodium glucose transporter-2 inhibitor (SGLT2i) treatment promotes urinary glucose excretion, thereby reducing blood glucose as well as body weight. However, only limited body weight reductions are achieved with SGLT2i administration. ... ...

Abstract	Selective sodium glucose transporter-2 inhibitor (SGLT2i) treatment promotes urinary glucose excretion, thereby reducing blood glucose as well as body weight. However, only limited body weight reductions are achieved with SGLT2i administration. Hyperphagia is reportedly one of the causes of this limited weight loss. However, the effects of SGLT2i on systemic energy expenditure have not been fully elucidated. We investigated the acute effects of dapagliflozin, an SGLT2i, on systemic energy expenditure in mice. Eighteen hours after dapagliflozin administration, oxygen consumption and brown adipose tissue (BAT) expression of ucp1, a thermogenesis-related gene, were significantly decreased as compared with those after vehicle administration. In addition, dapagliflozin significantly suppressed norepinephrine (NE) turnover in BAT and c-fos expression in the rostral raphe pallidus nucleus (rRPa), which contains the sympathetic premotor neurons responsible for thermogenesis. These findings indicate that the dapagliflozin-mediated acute decrease in energy expenditure involves a reduction in BAT thermogenesis via decreased sympathetic nerve activity from the rRPa. Furthermore, common hepatic branch vagotomy abolished the reductions in ucp1 expression, NE contents in BAT, and c-fos expression in the rRPa. In addition, alterations in hepatic carbohydrate metabolism, such as decreases in glycogen contents and upregulation of phosphoenolpyruvate carboxykinase, occurred prior to the suppression of BAT thermogenesis, e.g., 6 h after dapagliflozin treatment. Collectively, these results suggest that SGLT2i acutely suppresses energy expenditure in BAT via regulation of an interorgan neural network consisting of the common hepatic vagal branch and sympathetic nerves.
MeSH term(s)	Adipose Tissue, Brown/metabolism ; Animals ; Benzhydryl Compounds/administration & dosage ; Benzhydryl Compounds/pharmacology ; Energy Metabolism/drug effects ; Gene Expression/drug effects ; Glucosides/administration & dosage ; Glucosides/pharmacology ; Humans ; Liver/innervation ; Mice ; Midbrain Raphe Nuclei/metabolism ; Nerve Net/physiology ; Norepinephrine/metabolism ; Oxygen Consumption/drug effects ; Proto-Oncogene Proteins c-fos/genetics ; Proto-Oncogene Proteins c-fos/metabolism ; Signal Transduction/drug effects ; Sodium-Glucose Transporter 2 ; Sodium-Glucose Transporter 2 Inhibitors ; Sympathetic Nervous System/physiology ; Thermogenesis/genetics ; Uncoupling Protein 1/genetics ; Uncoupling Protein 1/metabolism ; Vagus Nerve/physiology
Chemical Substances	Benzhydryl Compounds ; Glucosides ; Proto-Oncogene Proteins c-fos ; Slc5a2 protein, mouse ; Sodium-Glucose Transporter 2 ; Sodium-Glucose Transporter 2 Inhibitors ; Ucp1 protein, mouse ; Uncoupling Protein 1 ; dapagliflozin (1ULL0QJ8UC) ; Norepinephrine (X4W3ENH1CV)
Language	Japanese
Publishing date	2018-06-14
Publishing country	Japan
Document type	Journal Article ; Review
ZDB-ID	200514-1
ISSN	1347-5231 ; 0031-6903 ; 0372-7750 ; 0919-2085 ; 0919-2131
ISSN (online)	1347-5231
ISSN	0031-6903 ; 0372-7750 ; 0919-2085 ; 0919-2131
DOI	10.1248/yakushi.17-00223-3
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Article ; Online: Life concerns of elderly people living at home determined as by Community General Support Center staff: implications for organizing a more effective integrated community care system. The Kurihara Project.

Takada, Junko / Meguro, Kenichi / Sato, Yuko / Chiba, Yumiko

Psychogeriatrics : the official journal of the Japanese Psychogeriatric Society

2014 Volume 14, Issue 3, Page(s) 188–195

Abstract: Background: In Japan, the integrated community care system aims to enable people to continue to live in their homes. Based on the concept, one of the activities of a Community General Support Center (CGSC) is to provide preventive intervention based on ... ...

Abstract	Background: In Japan, the integrated community care system aims to enable people to continue to live in their homes. Based on the concept, one of the activities of a Community General Support Center (CGSC) is to provide preventive intervention based on a Community Support Program. Currently, a Basic Checklist (BC) is sent to elderly people to identify persons appropriate for a Secondary Prevention Program. Methods: To find people who had not responded to the BC, CGSC staff evaluated the files of 592 subjects who had participated in the Kurihara Project to identify activities they cannot do that they did in the past, decreased activity levels at home, loss of interaction with people other than their family, and the need for medical interventions. This information was classified, when applicable, into the following categories: (A) 'no life concerns'; (B) 'undecided'; and (C) 'life concerns'. The relationships between these classifications and clinical information, certified need for long-term care, and items on the BC were examined. Results: The numbers of subjects in categories A, B, and C were 291, 42, and 186, respectively. Life concerns were related to scores on the Clinical Dementia Rating, global cognitive function, depressive state, and apathy. Most items on the BC were not associated with classification into category C, but ≥25% of the subjects had life concerns related to these items. Discussion: Assessment of life concerns by the CGSC staff has clinical validity. The results suggest that there are people who do not respond to the checklist or apply for Long-Term Care Insurance, meaning that they 'hide' in the community, probably due to apathy or depressive state. To organize a more effective integrated community care system, the CGSC staff should focus mainly on preventive care.
MeSH term(s)	Activities of Daily Living ; Aged ; Aged, 80 and over ; Dementia/psychology ; Female ; Health Services Needs and Demand ; Health Services for the Aged/organization & administration ; Home Care Services ; Humans ; Insurance, Long-Term Care ; Japan ; Long-Term Care/organization & administration ; Male ; Outcome Assessment (Health Care) ; Psychiatric Status Rating Scales ; Residence Characteristics ; Retrospective Studies
Language	English
Publishing date	2014-09
Publishing country	England
Document type	Journal Article
ZDB-ID	2213105-X
ISSN	1479-8301 ; 1346-3500
ISSN (online)	1479-8301
ISSN	1346-3500
DOI	10.1111/psyg.12061
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Article: [Influence of protamine sulfate as neutralizer of heparin on a vancomycin assay in serum].

Sasaki, Miho / Horiguchi, Sanae / Chiba, Yumiko

Rinsho byori. The Japanese journal of clinical pathology

2011 Volume 59, Issue 7, Page(s) 656–661

Abstract: Levels of vancomycin (VCM), measured with cobas 6000 c501(c501), were low when blood-collecting tubes for heparinized blood (SQH) were used. It was determined that the phenomenon was due to the effects of protamine sulfate, a heparin neutralizer. VCM ... ...

Abstract	Levels of vancomycin (VCM), measured with cobas 6000 c501(c501), were low when blood-collecting tubes for heparinized blood (SQH) were used. It was determined that the phenomenon was due to the effects of protamine sulfate, a heparin neutralizer. VCM levels decreased by approximately 10% when the concentration of protamine sulfate was 0.01 mg/ml, and were undetectable when the concentration was 0.045 mg/ml. However, the effects of protamine sulfate, with the dose being up to approximately twice, were not seen in the presence of heparin. There were no such effects if specimens in SQH were maintained at a specified volume. The phenomenon was characteristic of the measurement of VCM levels using c501. Measurements of other agents such as valproic acid, which is measured in the same manner as VCM, using the same equipment did not lead to the identification of any effect. In addition, when VCM levels were measured with reagents of INTEGRA 800, no effect was seen. It is difficult to elucidate the mechanisms of action, as the manufacturer has not provided detailed information regarding reagents. Protamine sulfate is estimated to partially influence the antigen-antibody reaction involving anti-VCM antibodies in reagents of c501. Protamine sulfate is also used as an injection, and, hence, the influence of the agent on VCM levels measured with c501 cannot be ruled out even if other blood collecting tubes are used. Attachment to separating mediums presents problems when measuring blood concentrations of drugs, but there has been no report regarding a heparin neutralizer, as seen in this case. This is a new influential factor that requires attention.
MeSH term(s)	Anti-Bacterial Agents/blood ; Blood Specimen Collection/instrumentation ; Blood Specimen Collection/methods ; Heparin ; Heparin Antagonists ; Humans ; Protamines ; Vancomycin/blood
Chemical Substances	Anti-Bacterial Agents ; Heparin Antagonists ; Protamines ; Vancomycin (6Q205EH1VU) ; Heparin (9005-49-6)
Language	Japanese
Publishing date	2011-07
Publishing country	Japan
Document type	English Abstract ; Journal Article
ZDB-ID	604196-6
ISSN	0047-1860 ; 0485-1404
ISSN	0047-1860 ; 0485-1404
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Article ; Online: Olfactory receptors are expressed in pancreatic β-cells and promote glucose-stimulated insulin secretion.

Munakata, Yuichiro / Yamada, Tetsuya / Imai, Junta / Takahashi, Kei / Tsukita, Sohei / Shirai, Yuta / Kodama, Shinjiro / Asai, Yoichiro / Sugisawa, Takashi / Chiba, Yumiko / Kaneko, Keizo / Uno, Kenji / Sawada, Shojiro / Hatakeyama, Hiroyasu / Kanzaki, Makoto / Miyazaki, Jun-Ichi / Oka, Yoshitomo / Katagiri, Hideki

Scientific reports

2018 Volume 8, Issue 1, Page(s) 1499

Abstract: Olfactory receptors (ORs) mediate olfactory chemo-sensation in OR neurons. Herein, we have demonstrated that the OR chemo-sensing machinery functions in pancreatic β-cells and modulates insulin secretion. First, we found several OR isoforms, including ... ...

Abstract	Olfactory receptors (ORs) mediate olfactory chemo-sensation in OR neurons. Herein, we have demonstrated that the OR chemo-sensing machinery functions in pancreatic β-cells and modulates insulin secretion. First, we found several OR isoforms, including OLFR15 and OLFR821, to be expressed in pancreatic islets and a β-cell line, MIN6. Immunostaining revealed OLFR15 and OLFR821 to be uniformly expressed in pancreatic β-cells. In addition, mRNAs of Olfr15 and Olfr821 were detected in single MIN6 cells. These results indicate that multiple ORs are simultaneously expressed in individual β-cells. Octanoic acid, which is a medium-chain fatty acid contained in food and reportedly interacts with OLFR15, potentiated glucose-stimulated insulin secretion (GSIS), thereby improving glucose tolerance in vivo. GSIS potentiation by octanoic acid was confirmed in isolated pancreatic islets and MIN6 cells and was blocked by OLFR15 knockdown. While Gα
MeSH term(s)	Animals ; Cell Line ; Gene Expression Profiling ; Glucose/metabolism ; Immunohistochemistry ; Insulin/metabolism ; Insulin-Secreting Cells/chemistry ; Insulin-Secreting Cells/drug effects ; Mice ; Mice, Inbred C57BL ; RNA, Messenger/analysis ; Receptors, Odorant/analysis ; Receptors, Odorant/genetics
Chemical Substances	Insulin ; Olfr256 protein, mouse ; Olfr821 protein, mouse ; RNA, Messenger ; Receptors, Odorant ; Glucose (IY9XDZ35W2)
Language	English
Publishing date	2018-01-24
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-018-19765-5
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Article ; Online: Dapagliflozin, a Sodium-Glucose Co-Transporter 2 Inhibitor, Acutely Reduces Energy Expenditure in BAT via Neural Signals in Mice.

Chiba, Yumiko / Yamada, Tetsuya / Tsukita, Sohei / Takahashi, Kei / Munakata, Yuichiro / Shirai, Yuta / Kodama, Shinjiro / Asai, Yoichiro / Sugisawa, Takashi / Uno, Kenji / Sawada, Shojiro / Imai, Junta / Nakamura, Kazuhiro / Katagiri, Hideki

PloS one

2016 Volume 11, Issue 3, Page(s) e0150756

Abstract: Selective sodium glucose cotransporter-2 inhibitor (SGLT2i) treatment promotes urinary glucose excretion, thereby reducing blood glucose as well as body weight. However, only limited body weight reductions are achieved with SGLT2i treatment. Hyperphagia ... ...

Abstract	Selective sodium glucose cotransporter-2 inhibitor (SGLT2i) treatment promotes urinary glucose excretion, thereby reducing blood glucose as well as body weight. However, only limited body weight reductions are achieved with SGLT2i treatment. Hyperphagia is reportedly one of the causes of this limited weight loss. However, the effects of SGLT2i treatment on systemic energy expenditure have not been fully elucidated. Herein, we investigated the acute effects of dapagliflozin, a SGLT2i, on systemic energy expenditure in mice. Eighteen hours after dapagliflozin treatment oxygen consumption and brown adipose tissue (BAT) expression of ucp1, a thermogenesis-related gene, were significantly decreased as compared to those after vehicle treatment. In addition, dapagliflozin significantly suppressed norepinephrine (NE) turnover in BAT and c-fos expression in the rostral raphe pallidus nucleus (rRPa) which contains the sympathetic premotor neurons responsible for thermogenesis. These findings indicate that the dapagliflozin-mediated acute decrease in energy expenditure involves a reduction in BAT thermogenesis via decreased sympathetic nerve activity from the rRPa. Furthermore, common hepatic branch vagotomy abolished the reductions in ucp1 expression and NE contents in BAT and c-fos expression in the rRPa. In addition, alterations in hepatic carbohydrate metabolism, such as decreases in glycogen contents and upregulation of phosphoenolpyruvate carboxykinase, manifested prior to the suppression of BAT thermogenesis, e.g. 6 hours after dapagliflozin treatment. Collectively, these results suggest that SGLT2i treatment acutely suppresses energy expenditure in BAT via regulation of an inter-organ neural network consisting of the common hepatic vagal branch and sympathetic nerves.
MeSH term(s)	Adipose Tissue, Brown/metabolism ; Animals ; Benzhydryl Compounds/pharmacology ; Carbohydrate Metabolism/drug effects ; Energy Metabolism/drug effects ; Gene Expression Regulation/drug effects ; Glucosides/pharmacology ; Glycogen/metabolism ; Ion Channels/biosynthesis ; Liver/metabolism ; Male ; Mice ; Midbrain Raphe Nuclei/metabolism ; Mitochondrial Proteins/biosynthesis ; Proto-Oncogene Proteins c-fos/biosynthesis ; Sodium-Glucose Transporter 2/metabolism ; Sodium-Glucose Transporter 2 Inhibitors ; Synaptic Transmission/drug effects ; Thermogenesis/drug effects ; Uncoupling Protein 1 ; Vagus Nerve/metabolism
Chemical Substances	Benzhydryl Compounds ; Glucosides ; Ion Channels ; Mitochondrial Proteins ; Proto-Oncogene Proteins c-fos ; Slc5a2 protein, mouse ; Sodium-Glucose Transporter 2 ; Sodium-Glucose Transporter 2 Inhibitors ; Ucp1 protein, mouse ; Uncoupling Protein 1 ; dapagliflozin (1ULL0QJ8UC) ; Glycogen (9005-79-2)
Language	English
Publishing date	2016-03-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0150756
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Article ; Online: MicroRNAs 106b and 222 Improve Hyperglycemia in a Mouse Model of Insulin-Deficient Diabetes via Pancreatic β-Cell Proliferation.

Tsukita, Sohei / Yamada, Tetsuya / Takahashi, Kei / Munakata, Yuichiro / Hosaka, Shinichiro / Takahashi, Hironobu / Gao, Junhong / Shirai, Yuta / Kodama, Shinjiro / Asai, Yoichiro / Sugisawa, Takashi / Chiba, Yumiko / Kaneko, Keizo / Uno, Kenji / Sawada, Shojiro / Imai, Junta / Katagiri, Hideki

EBioMedicine

2016 Volume 15, Page(s) 163–172

Abstract: Major symptoms of diabetes mellitus manifest, once pancreatic β-cell numbers have become inadequate. Although natural regeneration of β-cells after injury is very limited, bone marrow (BM) transplantation (BMT) promotes their regeneration through ... ...

Abstract	Major symptoms of diabetes mellitus manifest, once pancreatic β-cell numbers have become inadequate. Although natural regeneration of β-cells after injury is very limited, bone marrow (BM) transplantation (BMT) promotes their regeneration through undetermined mechanism(s) involving inter-cellular (BM cell-to-β-cell) crosstalk. We found that two microRNAs (miRNAs) contribute to BMT-induced β-cell regeneration. Screening murine miRNAs in serum exosomes after BMT revealed 42 miRNAs to be increased. Two of these miRNAs (miR-106b-5p and miR-222-3p) were shown to be secreted by BM cells and increased in pancreatic islet cells after BMT. Treatment with the corresponding anti-miRNAs inhibited BMT-induced β-cell regeneration. Furthermore, intravenous administration of the corresponding miRNA mimics promoted post-injury β-cell proliferation through Cip/Kip family down-regulation, thereby ameliorating hyperglycemia in mice with insulin-deficient diabetes. Thus, these identified miRNAs may lead to the development of therapeutic strategies for diabetes.
MeSH term(s)	Animals ; Bone Marrow Cells/metabolism ; Bone Marrow Transplantation ; Calcium-Binding Proteins/genetics ; Carrier Proteins/genetics ; Cell Proliferation ; Diabetes Mellitus, Experimental ; Diabetes Mellitus, Type 1/blood ; Diabetes Mellitus, Type 1/genetics ; Disease Models, Animal ; Exosomes ; Gene Expression Regulation ; Hyperglycemia/genetics ; Insulin-Secreting Cells/metabolism ; Islets of Langerhans/metabolism ; Mice ; MicroRNAs/genetics ; RNA Interference ; Regeneration
Chemical Substances	Calcium-Binding Proteins ; Carrier Proteins ; Cib1 protein, mouse ; MIRN222 microRNA, mouse ; MLIP protein, mouse ; MicroRNAs ; Mirn106 microRNA, mouse
Language	English
Publishing date	2016-12-07
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2851331-9
ISSN	2352-3964
ISSN (online)	2352-3964
DOI	10.1016/j.ebiom.2016.12.002
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Article ; Online: Activation of the Hypoxia Inducible Factor 1α Subunit Pathway in Steatotic Liver Contributes to Formation of Cholesterol Gallstones.

Asai, Yoichiro / Yamada, Tetsuya / Tsukita, Sohei / Takahashi, Kei / Maekawa, Masamitsu / Honma, Midori / Ikeda, Masanori / Murakami, Keigo / Munakata, Yuichiro / Shirai, Yuta / Kodama, Shinjiro / Sugisawa, Takashi / Chiba, Yumiko / Kondo, Yasuteru / Kaneko, Keizo / Uno, Kenji / Sawada, Shojiro / Imai, Junta / Nakamura, Yasuhiro /

Yamaguchi, Hiroaki / Tanaka, Kozo / Sasano, Hironobu / Mano, Nariyasu / Ueno, Yoshiyuki / Shimosegawa, Tooru / Katagiri, Hideki

Gastroenterology

2017 Volume 152, Issue 6, Page(s) 1521–1535.e8

Abstract: Background & aims: Hypoxia-inducible factor 1α subunit (HIF1A) is a transcription factor that controls the cellular response to hypoxia and is activated in hepatocytes of patients with nonalcoholic fatty liver disease (NAFLD). NAFLD increases the risk ... ...

Abstract	Background & aims: Hypoxia-inducible factor 1α subunit (HIF1A) is a transcription factor that controls the cellular response to hypoxia and is activated in hepatocytes of patients with nonalcoholic fatty liver disease (NAFLD). NAFLD increases the risk for cholesterol gallstone disease by unclear mechanisms. We studied the relationship between HIF1A and gallstone formation associated with liver steatosis. Methods: We performed studies with mice with inducible disruption of Hif1a in hepatocytes via a Cre adenoviral vector (inducible hepatocyte-selective HIF1A knockout [iH-HIFKO] mice), and mice without disruption of Hif1a (control mice). Mice were fed a diet rich in cholesterol and cholate for 1 or 2 weeks; gallbladders were collected and the number of gallstones was determined. Livers and biliary tissues were analyzed by histology, quantitative reverse-transcription polymerase chain reaction, immunohistochemistry, and immunoblots. We measured concentrations of bile acid, cholesterol, and phospholipid in bile and rates of bile flow. Primary hepatocytes and cholangiocytes were isolated and analyzed. HIF1A was knocked down in Hepa1-6 cells with small interfering RNAs. Liver biopsy samples from patients with NAFLD, with or without gallstones, were analyzed by quantitative reverse-transcription polymerase chain reaction. Results: Control mice fed a diet rich in cholesterol and cholate developed liver steatosis with hypoxia; levels of HIF1A protein were increased in hepatocytes around central veins and 90% of mice developed cholesterol gallstones. Only 20% of the iH-HIFKO mice developed cholesterol gallstones. In iH-HIFKO mice, the biliary lipid concentration was reduced by 36%, compared with control mice, and bile flow was increased by 35%. We observed increased water secretion from hepatocytes into bile canaliculi to mediate these effects, resulting in suppression of cholelithogenesis. Hepatic expression of aquaporin 8 (AQP8) protein was 1.5-fold higher in iH-HIFKO mice than in control mice. Under hypoxic conditions, cultured hepatocytes increased expression of Hif1a, Hmox1, and Vegfa messenger RNAs (mRNAs), and down-regulated expression of AQP8 mRNA and protein; AQP8 down-regulation was not observed in cells with knockdown of HIF1A. iH-HIFKO mice had reduced inflammation and mucin deposition in the gallbladder compared with control mice. Liver tissues from patients with NAFLD with gallstones had increased levels of HIF1A, HMOX1, and VEGFA mRNAs, compared with livers from patients with NAFLD without gallstones. Conclusions: In steatotic livers of mice, hypoxia up-regulates expression of HIF1A, which reduces expression of AQP8 and concentrates biliary lipids via suppression of water secretion from hepatocytes. This promotes cholesterol gallstone formation. Livers from patients with NAFLD and gallstones express higher levels of HIF1A than livers from patients with NAFLD without gallstones.
MeSH term(s)	Animals ; Aquaporins/genetics ; Aquaporins/metabolism ; Bile/metabolism ; Bile Acids and Salts/metabolism ; Cholates/administration & dosage ; Cholesterol/metabolism ; Cholesterol, Dietary/administration & dosage ; Cholesterol, Dietary/metabolism ; Down-Regulation/genetics ; Female ; Gallbladder/pathology ; Gallstones/genetics ; Gallstones/metabolism ; Gallstones/pathology ; Heme Oxygenase-1/genetics ; Hepatocytes/metabolism ; Humans ; Hypoxia/metabolism ; Hypoxia-Inducible Factor 1, alpha Subunit/genetics ; Hypoxia-Inducible Factor 1, alpha Subunit/metabolism ; Inflammation/etiology ; Liver/metabolism ; Male ; Membrane Proteins/genetics ; Mice ; Mice, Knockout ; Mucins/metabolism ; Non-alcoholic Fatty Liver Disease/complications ; Non-alcoholic Fatty Liver Disease/metabolism ; RNA, Messenger/metabolism ; Signal Transduction ; Vascular Endothelial Growth Factor A/genetics ; Water/metabolism
Chemical Substances	Aquaporins ; Bile Acids and Salts ; Cholates ; Cholesterol, Dietary ; Hif1a protein, mouse ; Hypoxia-Inducible Factor 1, alpha Subunit ; Membrane Proteins ; Mucins ; RNA, Messenger ; Vascular Endothelial Growth Factor A ; aquaporin 8 ; vascular endothelial growth factor A, mouse ; Water (059QF0KO0R) ; Cholesterol (97C5T2UQ7J) ; Heme Oxygenase-1 (EC 1.14.14.18) ; Hmox1 protein, mouse (EC 1.14.14.18)
Language	English
Publishing date	2017-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	80112-4
ISSN	1528-0012 ; 0016-5085
ISSN (online)	1528-0012
ISSN	0016-5085
DOI	10.1053/j.gastro.2017.01.001
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Article ; Online: Establishment of a neutralization test involving reporter gene-expressing virus-like particles of tick-borne encephalitis virus.

Yoshii, Kentaro / Ikawa, Ayae / Chiba, Yumiko / Omori, Yuki / Maeda, Junko / Murata, Ryo / Kariwa, Hiroaki / Takashima, Ikuo

Journal of virological methods

2009 Volume 161, Issue 1, Page(s) 173–176

Abstract: Previously, a system for packaging tick-borne encephalitis virus (TBEV) subgenomic replicon RNAs into single-round infectious virus-like particles (VLPs) was developed. In the present study, VLPs were applied to measuring the levels of neutralizing ... ...

Abstract	Previously, a system for packaging tick-borne encephalitis virus (TBEV) subgenomic replicon RNAs into single-round infectious virus-like particles (VLPs) was developed. In the present study, VLPs were applied to measuring the levels of neutralizing antibodies against TBEV as an alternative to performing neutralization tests with live virus. As markers of VLP infection, the genes for GFP and luciferase were inserted into the TBEV replicon, which was then packaged into VLPs. The reporter genes were expressed in cells that were infected with the VLPs, and this infection was inhibited by neutralizing antibodies to TBEV. Serum samples from wild rodents were used to evaluate the neutralization test using VLPs. All the sera that were positive in the conventional neutralization test were also found to be positive in the neutralization test using VLPs, and there were highly significant correlations between the neutralization titres obtained using the native virus and those using VLPs. These results indicate that VLPs that express reporter genes represent a useful and safe alternative to conventional neutralization testing using live virus.
MeSH term(s)	Animals ; Antibodies, Viral/blood ; Encephalitis Viruses, Tick-Borne/genetics ; Encephalitis Viruses, Tick-Borne/immunology ; Genes, Reporter ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Luciferases/genetics ; Luciferases/metabolism ; Neutralization Tests/methods ; Rodentia ; Virosomes
Chemical Substances	Antibodies, Viral ; Virosomes ; enhanced green fluorescent protein ; Green Fluorescent Proteins (147336-22-9) ; Luciferases (EC 1.13.12.-)
Language	English
Publishing date	2009-10
Publishing country	Netherlands
Document type	Comparative Study ; Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	8013-5
ISSN	1879-0984 ; 0166-0934
ISSN (online)	1879-0984
ISSN	0166-0934
DOI	10.1016/j.jviromet.2009.05.016
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Article: Epizootiological study of tick-borne encephalitis virus infection in Japan.

Yoshii, Kentaro / Mottate, Keita / Omori-Urabe, Yuki / Chiba, Yumiko / Seto, Takahiro / Sanada, Takahiro / Maeda, Junko / Obara, Mayumi / Ando, Shuji / Ito, Naoto / Sugiyama, Makoto / Sato, Hiroshi / Fukushima, Hiroshi / Kariwa, Hiroaki / Takashima, Ikuo

The Journal of veterinary medical science

2010 Volume 73, Issue 3, Page(s) 409–412

Abstract: Tick-borne encephalitis virus (TBEV) is a zoonotic agent causing severe encephalitis in humans. Rodent species that are potential hosts for TBEV are widely distributed in various regions in Japan. In this study, we carried out large-scale ... ...

Abstract	Tick-borne encephalitis virus (TBEV) is a zoonotic agent causing severe encephalitis in humans. Rodent species that are potential hosts for TBEV are widely distributed in various regions in Japan. In this study, we carried out large-scale epizootiological surveys in rodents from various areas of Japan. A total of 931 rodent and insectivore sera were collected from field surveys. Rodents seropositive for TBEV were found in Shimane Prefecture in Honshu and in several areas of Hokkaido Prefecture. These results emphasize the need for further epizootiological and epidemiological research of TBEV and preventive measures for emerging tick-borne encephalitis in Japan.
MeSH term(s)	Animals ; Encephalitis Viruses, Tick-Borne ; Encephalitis, Tick-Borne/epidemiology ; Encephalitis, Tick-Borne/virology ; Humans ; Insectivora/blood ; Insectivora/virology ; Japan/epidemiology ; Rodentia/blood ; Rodentia/virology ; Zoonoses
Language	English
Publishing date	2010-11-02
Publishing country	Japan
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1071753-5
ISSN	1347-7439 ; 0916-7250
ISSN (online)	1347-7439
ISSN	0916-7250
DOI	10.1292/jvms.10-0350
Database	MEDical Literature Analysis and Retrieval System OnLINE

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