LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 6 of total 6

Search options

  1. Article ; Online: Establishment and characterization of transformed goat primary cells by expression of simian virus 40 large T antigen for orf virus propagations.

    Yumiko Yamada / Guan-Ru Liao / Ching-Yu Tseng / Yeu-Yang Tseng / Wei-Li Hsu

    PLoS ONE, Vol 14, Iss 12, p e

    2019  Volume 0226105

    Abstract: Due to the limited host range of orf virus (ORFV), primary cells derived from its natural hosts, such as goats and sheep, are recommended for isolation and propagation of wild type ORFV. This situation limits the option for the study of virus-host ... ...

    Abstract Due to the limited host range of orf virus (ORFV), primary cells derived from its natural hosts, such as goats and sheep, are recommended for isolation and propagation of wild type ORFV. This situation limits the option for the study of virus-host interaction during ORFV infection since primary cells only support a few numbers of passages. SV40 T antigen is a viral oncoprotein that can abrogate replicative senescence, leading to an extended life span of cells. In this study, the transformation of two goat primary cells, fibroblast (FB) and testis (GT) cells, were achieved by stably expressing SV40 T antigen using the lentiviral technique. The presence of the gene encoding SV40 T antigen was validated by polymerase chain reaction (PCR) and western blot analyses. As evidenced by immunofluorescent microscopy, the two types of cells expressing SV40 T antigen (namely, FBT and GTT) were purified to homogeneity. Moreover, faster growth kinetics and a lower serum dependency were noticed in FBT and GTT, as compared with their counterpart parental cells. FBT and GTT remain permissive and can form plaque of ORFV, despite with different profiles; generally speaking, with SV40 T expression, ORFV forms plaques with smaller size and distinct margin. Most importantly, the prolonged life span of goat FBT and GTT serves as an ideal cell culture resource for ORFV isolation from the field, studies of ORFV pathogenesis and efficient vaccine development.
    Keywords Medicine ; R ; Science ; Q
    Subject code 570
    Language English
    Publishing date 2019-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Article ; Online: The Association between Physical Fitness Performance and Subjective Happiness among Taiwanese Adults

    Hui-Ling Chen / Po-Fu Lee / Yun-Chi Chang / Fu-Shu Hsu / Ching-Yu Tseng / Xin-Yu Hsieh / Chien-Chang Ho

    International Journal of Environmental Research and Public Health, Vol 17, Iss 3774, p

    2020  Volume 3774

    Abstract: The purpose of the present study was to determine the association between health-related physical fitness (HRPF) performance and perceived happiness status among adults in Taiwan. A cross-sectional study was conducted, and data derived from the National ... ...

    Abstract The purpose of the present study was to determine the association between health-related physical fitness (HRPF) performance and perceived happiness status among adults in Taiwan. A cross-sectional study was conducted, and data derived from the National Physical Fitness Survey in Taiwan 2014–2015 were reviewed. The participants included 27,930 men and 30,885 women, aged 23 to 64 years. Each participant completed a standardized, structured questionnaire and underwent anthropometric variable and HRPF measurements. The happiness outcome of an individual was obtained using the questionnaire, and the results were stratified into happy (very happy, quite happy, and fair) and unhappy (unhappy and not at all happy) groups for perceived happiness status. HRPF measurements were evaluated using cardiorespiratory endurance (3 min step test), muscle strength and endurance (1 min sit-up test), flexibility (sit-and-reach test), and body composition (body mass index (BMI) and waist-to-hip ratio). To determine the existence of a dose–response relationship between HRPF component levels and happiness status, four quartiles of HRPF components were analyzed using multiple logistic regression models. Multiple logistic regression results indicated that with the worst performance level of HRPF components as a baseline, significant associations were observed for the sit-and-reach test (third level: OR = 1.24, 95% CI: 1.02–1.49) and BMI (second level: OR = 0.78, 95% CI: 0.64–0.95) among men. For women, significant associations were observed for the 1 min sit-up test (second level: OR = 1.28, 95% CI: 1.03–1.60; third level: OR = 1.32, 95% CI: 1.04–1.67; fourth (the best) level: OR = 1.48, 95% CI: 1.12–1.95) and BMI (third level: OR = 0.73, 95% CI: 0.58–0.92). The current study suggested that higher values in flexibility and body composition, happiness-related factors, potentially improve the occurrence of happiness among men. Moreover, this positive effect of higher values of muscle strength, endurance, and BMI was observed for the ...
    Keywords physical activity ; health related physical fitness ; quality of life ; mental health ; Medicine ; R
    Subject code 796
    Language English
    Publishing date 2020-05-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article ; Online: Coenzyme Q10 status, glucose parameters, and antioxidative capacity in college athletes

    Chien-Chang Ho / Ching-Yu Tseng / Hung-Wun Chen / Yi-Wen Chiu / Ming-Chih Tsai / Po-Sheng Chang / Ping-Ting Lin

    Journal of the International Society of Sports Nutrition, Vol 17, Iss 1, Pp 1-

    2020  Volume 8

    Abstract: Abstract Background Glycemia is related to energy production during exercise. Coenzyme Q10 is an antioxidant that participates in adenosine triphosphate synthesis in mitochondria. The aim of this study was to investigate the level of coenzyme Q10, ... ...

    Abstract Abstract Background Glycemia is related to energy production during exercise. Coenzyme Q10 is an antioxidant that participates in adenosine triphosphate synthesis in mitochondria. The aim of this study was to investigate the level of coenzyme Q10, glucose parameters, and antioxidant capacity in athletes. Methods This study was designed as a cross-sectional study. Well-trained college athletes (n = 43) and age-gender matched healthy subjects (n = 25) were recruited from a college. The levels of glucose parameters, oxidative stress, antioxidant enzymes activity, Trolox equivalent antioxidant capacity (TAC), and coenzyme Q10 status were measured in the present study. Results The athletes had a significantly lower level of white blood cells (WBC) coenzyme Q10 than the healthy subjects (0.34 ± 0.24 vs. 0.65 ± 0.43 nmol/g, p < 0.01); however, no significant difference was detected in plasma coenzyme Q10 between the two groups. Regarding the glucose parameters, the athletes had significantly higher values for HbA1c (5.5 ± 0.3 vs. 5.3 ± 0.3%, p < 0.05) and quantitative insulin sensitivity check index (QUICKI, 0.37 ± 0.03 vs. 0.34 ± 0.03, p < 0.05), and lower homeostatic model assessment-insulin resistance (HOMA-IR, 1.5 ± 0.8 vs. 2.9 ± 3.8, p < 0.05) than the healthy subjects. A higher level of TAC was found in the athletes (serum, 5.7 ± 0.3 vs. 5.4 ± 0.2 mM Trolox; erythrocyte, 10.5 ± 0.6 vs. 10.0 ± 0.5 mM Trolox, p < 0.05). In addition, WBC coenzyme Q10 status was significantly correlated with catalase activity (r = 0.56, p < 0.01), GPx activity (r = 0.56, p < 0.01), serum TAC (r = 0.54, p < 0.01), fasting glucose (β = − 1.10, p < 0.01), HbA1c (β = − 0.82, p < 0.01), HOMA-IR (β = − 1.81, p < 0.01), and QUICK (β = 0.08, p < 0.01). Conclusions Athletes may suffer from a marginal coenzyme Q10 deficiency, and the level was related to glycemic control and antioxidant capacity. Further interventional studies are needed to clarify an adequate dose of coenzyme Q10 supplementation in athletes ...
    Keywords Athletes ; Coenzyme Q10 ; Glucose parameters ; Antioxidant capacity ; Sport nutrition ; Nutrition. Foods and food supply ; TX341-641 ; Sports medicine ; RC1200-1245
    Subject code 796
    Language English
    Publishing date 2020-01-01T00:00:00Z
    Publisher Taylor & Francis Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  4. Article ; Online: The Association between Physical Fitness Performance and Abdominal Obesity Risk among Taiwanese Adults

    Po-Fu Lee / Chien-Chang Ho / Nai-Wen Kan / Ding-Peng Yeh / Yun-Chi Chang / Yu-Jui Li / Ching-Yu Tseng / Xin-Yu Hsieh / Chih-Hui Chiu

    International Journal of Environmental Research and Public Health, Vol 17, Iss 5, p

    A Cross-Sectional Study

    2020  Volume 1722

    Abstract: The present study aims to investigate the associations between physical fitness performance and abdominal obesity risk among Taiwanese adults. We conducted a cross-sectional study and reviewed the data derived from the National Physical Fitness Survey in ...

    Abstract The present study aims to investigate the associations between physical fitness performance and abdominal obesity risk among Taiwanese adults. We conducted a cross-sectional study and reviewed the data derived from the National Physical Fitness Survey in Taiwan (HPFSIT). Data from a total of 62,486 respondents aged 23−64 years were collected in this study. The participants completed a standardized structural questionnaire and a series of anthropometric characteristics (body mass index and waist-to-hip ratio) and physical fitness (3-min step tests, 1-min sit-up tests, and sit-and-reach tests) assessments. Waist circumference was used to define abdominal obesity status. A multiple logistic regression analysis was conducted. Our results presented almost entirely significant associations (except for women) on the 3-min step test. Moreover, the results suggest that muscular strength, endurance, and flexibility may be effective predictors of abdominal obesity among men and women, whereas cardiorespiratory fitness predicted abdominal obesity only in men. According to the results of this study, the fitness−abdominal obesity associations are minor based on a mixed population analysis. However, dose−response relationships have been observed. The present study provides a new perspective by using different types of fitness performance to predict abdominal obesity.
    Keywords physical fitness performance ; abdominal obesity risk ; waist circumference ; taiwan ; Medicine ; R
    Subject code 796
    Language English
    Publishing date 2020-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  5. Article ; Online: Cross-Sectional Associations of Physical Fitness Performance Level and Sleep Duration among Older Adults

    Po-Fu Lee / Chien-Chang Ho / Ding-Peng Yeh / Chang-Tsen Hung / Yun-Chi Chang / Chia-Chen Liu / Ching-Yu Tseng / Xin-Yu Hsieh

    International Journal of Environmental Research and Public Health, Vol 17, Iss 2, p

    Results from the National Physical Fitness Survey in Taiwan

    2020  Volume 388

    Abstract: Research on relationships between physical fitness and sleep duration among older adults is scarce, especially in Taiwanese representative samples of elderly people who undergo physical fitness measurements. This study aimed to determine the associations ...

    Abstract Research on relationships between physical fitness and sleep duration among older adults is scarce, especially in Taiwanese representative samples of elderly people who undergo physical fitness measurements. This study aimed to determine the associations between physical fitness and short and long sleep durations among older adults in Taiwan. We conducted a cross-sectional study and reviewed data derived from the National Physical Fitness Survey in Taiwan. A total of 24,125 Taiwanese adults aged 65 years and older participated in this study between October 2014 and March 2015. Each individual’s sleep duration was recorded with a standard questionnaire method. Sleep duration data were stratified into short (≤5 h), normal (6−7 h), and long (≥8 h) sleep duration groups. Physical fitness was assessed by five components: aerobic endurance (2 min step test), muscle strength and endurance (30 s arm curl and 30 s chair stand tests), flexibility (back scratch and chair sit-and-reach tests), body composition (body mass index (BMI) and waist-to-hip ratio (WHR)), and balance (one-leg stance with eye open and 8-foot up-and-go tests). To understand whether a dose−response relationship exists between physical fitness and short or long sleep duration, we analyzed four levels of performance on the basis of quartiles of physical fitness measurements by using logistic regression. The first quartile of physical fitness performance was the baseline level. The odds ratio (OR) for short sleep duration for the third quartile of BMI was 0.8031 times (95% CI, 0.7119−0.9061) lower than the baseline. For the fourth quartile of BMI, the OR was 0.8660 times (95% CI, 0.7653−0.9800) lower than the baseline. The adjusted OR for long sleep duration significantly decreased in the second, third, and fourth quartiles of the 30 s chair stand, back scratch, chair sit-and-reach test, one-leg stance with one eye open, and BMI. The adjusted OR was increased in the third and fourth quartiles of the 8-foot up-and-go and WHR. The results of the current ...
    Keywords physical fitness ; sleep duration ; elderly ; taiwan ; Medicine ; R
    Subject code 796
    Language English
    Publishing date 2020-01-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  6. Article ; Online: Benefits of different intensity of aerobic exercise in modulating body composition among obese young adults

    Chih-Hui Chiu / Ming-Chen Ko / Long-Shan Wu / Ding-Peng Yeh / Nai-Wen Kan / Po-Fu Lee / Jenn-Woei Hsieh / Ching-Yu Tseng / Chien-Chang Ho

    Health and Quality of Life Outcomes, Vol 15, Iss 1, Pp 1-

    a pilot randomized controlled trial

    2017  Volume 9

    Abstract: Abstract Background The aim of present study was to compare the effects of different aerobic exercise intensities and energy expenditures on the body composition of sedentary obese college students in Taiwan. Methods Forty-eight obese participants [body ... ...

    Abstract Abstract Background The aim of present study was to compare the effects of different aerobic exercise intensities and energy expenditures on the body composition of sedentary obese college students in Taiwan. Methods Forty-eight obese participants [body mass index (BMI) ≥ 27 kg/m2, age 18–26 years] were randomized into four equal groups (n = 12): light-intensity training group (LITG), 40%–50% heart rate reserve (HRR); middle-intensity training group (MITG), 50%–70% HRR; high-intensity training group (HITG), 70%–80% HRR; and control group (CG). The aerobic exercise training program was conducted for 60 min per day on a treadmill 3 days per week for 12 weeks. All participant anthropometric data, blood biochemical parameters, and health-related physical fitness components were measured at baseline and after 12 weeks. Results At baseline, the anthropometric indices did not differ significantly among the four groups (p > 0.05). After 12-week exercise intervention, the HITG and MITG had significantly more changes in body weight, waist circumference (WC), waist-to-hip ratio (WHR), and waist-to-height ratio (WHtR) than the LITG. The changes in BMI and body fat percentage differed among all four groups (p < 0.05). Conclusions A 12-week high-intensity exercise intervention with high energy expenditure can considerably reduce body weight, body fat, WC, WHR, and WHtR, whereas a light-intensity exercise intervention can significantly reduce body weight and body fat. Trial registration Current Controlled Trials TPECTR09831410900 , registered on 24th Dec 2009.
    Keywords Obesity ; Aerobic exercise ; Exercise intensity ; Body composition ; Computer applications to medicine. Medical informatics ; R858-859.7
    Subject code 796
    Language English
    Publishing date 2017-08-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top