Article ; Online: RNA Editing Enzyme ADAR1 Suppresses the Mobility of Cancer Cells via ARPIN.
2023 Volume 46, Issue 6, Page(s) 351–359
Abstract: Deamination of adenine or cytosine in RNA, called RNA editing, is a constitutively active and common modification. The primary role of RNA editing is tagging RNA right after its synthesis so that the endogenous RNA is recognized as self and distinguished ...
| Abstract | Deamination of adenine or cytosine in RNA, called RNA editing, is a constitutively active and common modification. The primary role of RNA editing is tagging RNA right after its synthesis so that the endogenous RNA is recognized as self and distinguished from exogenous RNA, such as viral RNA. In addition to this primary function, the direct or indirect effects on gene expression can be utilized in cancer where a high level of RNA editing activity persists. This report identified actin-related protein 2/3 complex inhibitor (ARPIN) as a target of ADAR1 in breast cancer cells. Our comparative RNA sequencing analysis in MCF7 cells revealed that the expression of ARPIN was decreased upon ADAR1 depletion with altered editing on its 3'UTR. However, the expression changes of ARPIN were not dependent on 3'UTR editing but relied on three microRNAs acting on ARPIN. As a result, we found that the migration and invasion of cancer cells were profoundly increased by ADAR1 depletion, and this cellular phenotype was reversed by the exogenous ARPIN expression. Altogether, our data suggest that ADAR1 suppresses breast cancer cell mobility via the upregulation of ARPIN. |
|---|---|
| MeSH term(s) | 3' Untranslated Regions ; Adenosine Deaminase/genetics ; Adenosine Deaminase/metabolism ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Neoplasms/genetics ; RNA Editing ; RNA-Binding Proteins/genetics ; RNA-Binding Proteins/metabolism ; Humans ; Cell Line, Tumor ; Carrier Proteins/metabolism |
| Chemical Substances | 3' Untranslated Regions ; Adenosine Deaminase (EC 3.5.4.4) ; MicroRNAs ; RNA-Binding Proteins ; ADAR protein, human (EC 3.5.4.37) ; arpin protein, human ; Carrier Proteins |
| Language | English |
| Publishing date | 2023-03-16 |
| Publishing country | United States |
| Document type | Journal Article |
| ZDB-ID | 1148964-9 |
| ISSN | 0219-1032 ; 1016-8478 |
| ISSN (online) | 0219-1032 |
| ISSN | 1016-8478 |
| DOI | 10.14348/molcells.2023.2174 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
| Zs.A 4713: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.