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  1. Article ; Online: Unveiling host-guest-solvent interactions in solution by identifying highly unstable host-guest configurations in thermal non-equilibrium gas phase.

    Choi, Hyoju / Oh, Young-Ho / Park, Soojin / Lee, Sung-Sik / Oh, Han Bin / Lee, Sungyul

    Scientific reports

    2022  Volume 12, Issue 1, Page(s) 8169

    Abstract: We propose a novel scheme of examining the host-guest-solvent interactions in solution from their gas phase structures. By adopting the permethylated β-cyclodextrin (perm β-CD)-protonated L-Lysine non-covalent complex as a prototypical system, we present ...

    Abstract We propose a novel scheme of examining the host-guest-solvent interactions in solution from their gas phase structures. By adopting the permethylated β-cyclodextrin (perm β-CD)-protonated L-Lysine non-covalent complex as a prototypical system, we present the infrared multiple photon dissociation (IRMPD) spectrum of the gas phase complex produced by electrospray ionization technique. In order to elucidate the structure of perm β-CD)/LysH
    Language English
    Publishing date 2022-05-17
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-022-12226-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Copper-Catalyzed Regiodivergent Internal Allylic Alkylations.

    Manna, Madhu Sudan / Yoo, Seok Yeol / Sharique, Mohammed / Choi, Hyoju / Pudasaini, Bimal / Baik, Mu-Hyun / Tambar, Uttam K

    Angewandte Chemie (International ed. in English)

    2023  Volume 62, Issue 35, Page(s) e202304848

    Abstract: We report a copper-catalyzed, regioselective, and stereospecific alkylation of unbiased internal allylic carbonates with functionalized alkyl and aryl Grignard reagents. The reactions exhibit high stereospecificity and regioselectivity for either ... ...

    Abstract We report a copper-catalyzed, regioselective, and stereospecific alkylation of unbiased internal allylic carbonates with functionalized alkyl and aryl Grignard reagents. The reactions exhibit high stereospecificity and regioselectivity for either S
    Language English
    Publishing date 2023-07-17
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2011836-3
    ISSN 1521-3773 ; 1433-7851
    ISSN (online) 1521-3773
    ISSN 1433-7851
    DOI 10.1002/anie.202304848
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Harnessing Ionic Interactions and Hydrogen Bonding for Nucleophilic Fluorination.

    Oh, Young-Ho / Choi, Hyoju / Park, Chanho / Kim, Dong Wook / Lee, Sungyul

    Molecules (Basel, Switzerland)

    2020  Volume 25, Issue 3

    Abstract: We review recent works for nucleophilic fluorination of organic compounds in which the Coulombic interactions between ionic species and/or hydrogen bonding affect the outcome of the reaction. ... ...

    Abstract We review recent works for nucleophilic fluorination of organic compounds in which the Coulombic interactions between ionic species and/or hydrogen bonding affect the outcome of the reaction. S
    MeSH term(s) Anions/chemistry ; Cations/chemistry ; Fluorides/chemistry ; Guanidine/chemistry ; Hydrogen Bonding ; Ionic Liquids/chemistry ; Metals, Alkali/chemistry ; Solvents/chemistry
    Chemical Substances Anions ; Cations ; Ionic Liquids ; Metals, Alkali ; Solvents ; Guanidine (JU58VJ6Y3B) ; Fluorides (Q80VPU408O)
    Language English
    Publishing date 2020-02-07
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules25030721
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.MO 81: Show issues

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  4. Article ; Online: Enhanced cell growth, production, and mAb quality produced in Chinese hamster ovary-K1 cells by supplementing polyamine in the media.

    Kang, Da Eun / An, Yeong Bin / Kim, Yeunju / Ahn, Seawon / Kim, Young Jin / Lim, Jung Soo / Ryu, Soo Hyun / Choi, Hyoju / Yoo, Jiseon / You, Weon-Kyoo / Lee, Dong-Yup / Park, Junsoo / Hong, Minsun / Lee, Gyun Min / Baik, Jong Youn / Hong, Jong Kwang

    Applied microbiology and biotechnology

    2023  Volume 107, Issue 9, Page(s) 2855–2870

    Abstract: Polyamines such as putrescine (PUT), spermidine (SPD), and spermine (SPM) are amine group-containing biomolecules that regulate multiple intracellular functions such as proliferation, differentiation, and stress response in mammalian cells. Although ... ...

    Abstract Polyamines such as putrescine (PUT), spermidine (SPD), and spermine (SPM) are amine group-containing biomolecules that regulate multiple intracellular functions such as proliferation, differentiation, and stress response in mammalian cells. Although these biomolecules can be generated intracellularly, lack of polyamine-synthesizing activity has occasionally been reported in a few mammalian cell lines such as Chinese hamster ovary (CHO)-K1; thus, polyamine supplementation in serum-free media is required to support cell growth and production. In the present study, the effects of biogenic polyamines PUT, SPD, and SPM in media on cell growth, production, metabolism, and antibody quality were explored in cultures of antibody-producing CHO-K1 cells. Polyamine withdrawal from media significantly suppressed cell growth and production. On the other hand, enhanced culture performance was achieved in polyamine-containing media conditions in a dose-dependent manner regardless of polyamine type. In addition, in polyamine-deprived medium, distinguishing metabolic features, such as enriched glycolysis and suppressed amino acid consumption, were observed and accompanied by higher heterogeneity of antibody quality compared with the optimal concentration of polyamines. Furthermore, an excessive concentration of polyamines negatively affected culture performance as well as antibody quality. Hence, the results suggest that polyamine-related metabolism needs to be further investigated and polyamines in cell growth media should be optimized as a controllable parameter in CHO cell culture bioprocessing. KEY POINTS: • Polyamine supplementation enhanced cell growth and production in a dose-dependent manner • Polyamine type and concentration in the media affected mAb quality • Optimizing polyamines in the media is suggested in CHO cell bioprocessing.
    MeSH term(s) Cricetinae ; Animals ; Polyamines/pharmacology ; Polyamines/metabolism ; CHO Cells ; Cricetulus ; Spermidine/metabolism ; Putrescine/pharmacology ; Putrescine/metabolism ; Spermine/metabolism ; Spermine/pharmacology ; Cell Proliferation
    Chemical Substances Polyamines ; Spermidine (U87FK77H25) ; Putrescine (V10TVZ52E4) ; Spermine (2FZ7Y3VOQX)
    Language English
    Publishing date 2023-03-22
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 392453-1
    ISSN 1432-0614 ; 0171-1741 ; 0175-7598
    ISSN (online) 1432-0614
    ISSN 0171-1741 ; 0175-7598
    DOI 10.1007/s00253-023-12459-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2229: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Silencing of an α-dioxygenase gene, Ca-DOX, retards growth and suppresses basal disease resistance responses in Capsicum annum.

    Hong, Chi Eun / Ha, Young-Im / Choi, Hyoju / Moon, Ju Yeon / Lee, Jiyoung / Shin, Ah-Young / Park, Chang Jin / Yoon, Gyeong Mee / Kwon, Suk-Yoon / Jo, Ick-Hyun / Park, Jeong Mee

    Plant molecular biology

    2017  Volume 93, Issue 4-5, Page(s) 497–509

    Abstract: Alpha-dioxygenases (α-DOX) catalyzing the primary oxygenation of fatty acids to oxylipins were recently found in plants. Here, the biological roles of the pepper α-DOX (Ca-DOX) gene, which is strongly induced during non-host pathogen infection in chili ... ...

    Abstract Alpha-dioxygenases (α-DOX) catalyzing the primary oxygenation of fatty acids to oxylipins were recently found in plants. Here, the biological roles of the pepper α-DOX (Ca-DOX) gene, which is strongly induced during non-host pathogen infection in chili pepper, were examined. Virus-induced gene silencing demonstrated that down-regulation of Ca-DOX enhanced susceptibility to bacterial pathogens and suppressed the hypersensitive response via the suppression of pathogenesis-related genes such as PR4, proteinase inhibitor II and lipid transfer protein (PR14). Ca-DOX-silenced pepper plants also exhibited more retarded growth with lower epidermal cell numbers and reduced cell wall thickness than control plants. To better understand regulation of Ca-DOX, transgenic Arabidopsis plants harboring the β-glucuronidase (GUS) reporter gene driven from a putative Ca-DOX promoter were generated. GUS expression was significantly induced upon avirulent pathogen infection in transgenic Arabidopsis leaves, whereas GUS induction was relatively weak upon virulent pathogen treatment. After treatment with plant hormones, early and strong GUS expression was seen after treatment of salicylic acid, whereas ethylene and methyl jasmonate treatments produced relatively weak and late GUS signals. These results will enable us to further understand the role of α-DOX, which is important in lipid metabolism, defense responses, and growth development in plants.
    MeSH term(s) Amino Acid Sequence ; Capsicum/genetics ; Capsicum/microbiology ; Dioxygenases/genetics ; Dioxygenases/metabolism ; Disease Resistance/genetics ; Gene Expression Regulation, Plant/drug effects ; Gene Silencing ; Host-Pathogen Interactions ; Microscopy, Electron, Transmission ; Plant Diseases/genetics ; Plant Diseases/microbiology ; Plant Growth Regulators/pharmacology ; Plant Leaves/genetics ; Plant Leaves/microbiology ; Plant Leaves/ultrastructure ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Plants, Genetically Modified ; Pseudomonas syringae/physiology ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Homology, Amino Acid ; Xanthomonas/physiology
    Chemical Substances Plant Growth Regulators ; Plant Proteins ; Dioxygenases (EC 1.13.11.-)
    Language English
    Publishing date 2017-03
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 778032-1
    ISSN 1573-5028 ; 0167-4412
    ISSN (online) 1573-5028
    ISSN 0167-4412
    DOI 10.1007/s11103-016-0575-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3458: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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