Article ; Online: Synthesis of Cationic Quaternized Nanolevan Derivative for Small Molecule and Nucleic Acid Delivery
Gels, Vol 9, Iss 188, p
2023 Volume 188
Abstract: Levan is a biopolymer composed of fructose chains covalently linked by β−2,6 glycosidic linkages. This polymer self−assembles into a nanoparticle of uniform size, making it useful for a wide range of applications. Also, levan exhibits various biological ... ...
| Abstract | Levan is a biopolymer composed of fructose chains covalently linked by β−2,6 glycosidic linkages. This polymer self−assembles into a nanoparticle of uniform size, making it useful for a wide range of applications. Also, levan exhibits various biological activities such as antioxidants, anti-inflammatory, and anti-tumor, that make this polymer very attractive for biomedical application. In this study, levan synthesized from Erwinia tasmaniensis was chemically modified by glycidyl trimethylammonium chloride (GTMAC) to produce cationized nanolevan (QA-levan). The structure of the obtained GTMAC−modified levan was determined by FT-IR, 1 H-NMR and elemental (CHN) analyzer. The size of the nanoparticle was calculated using the dynamic light scattering method (DLS). The formation of DNA/QA-levan polyplex was then investigated by gel electrophoresis. The modified levan was able to increase the solubility of quercetin and curcumin by 11-folds and 205-folds, respectively, compared to free compounds. Cytotoxicity of levan and QA−levan was also investigated in HEK293 cells. This finding suggests that GTMAC−modified levan should have a potential application for drug and nucleic acid delivery. |
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| Keywords | levan ; fructan ; exopolysaccharide ; drug delivery ; Science ; Q ; Chemistry ; QD1-999 ; Inorganic chemistry ; QD146-197 ; General. Including alchemy ; QD1-65 |
| Subject code | 540 |
| Language | English |
| Publishing date | 2023-02-01T00:00:00Z |
| Publisher | MDPI AG |
| Document type | Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
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