Article: Unusual phenotypic features in a patient with a novel splice mutation in the GHRHR gene.
Molecular medicine (Cambridge, Mass.)
2008 Volume 14, Issue 5-6, Page(s) 286–292
Abstract: Isolated growth hormone deficiency (IGHD) may be of genetic origin. One of the few genes involved in that condition encodes the growth hormone releasing hormone receptor (GHRHR) that, through its ligand (GHRH), plays a pivotal role in the GH synthesis ... ...
| Abstract | Isolated growth hormone deficiency (IGHD) may be of genetic origin. One of the few genes involved in that condition encodes the growth hormone releasing hormone receptor (GHRHR) that, through its ligand (GHRH), plays a pivotal role in the GH synthesis and secretion by the pituitary. Our objective is to describe the phenotype of two siblings born to a consanguineous union presenting with short stature (IGHD) and Magnetic Resonance Imaging (MRI) abnormalities, and to identify the molecular basis of this condition. Our main outcome measures were clinical and endocrinological investigations, MRI of the pituitary region, study of the GHRHR gene sequence and transcripts. In both patients, the severe growth retardation (-5SD) was combined with anterior pituitary hypoplasia. In addition to these classical phenotypic features for IGHD, one of the patients had a Chiari I malformation, an arachnoid cyst, and a dysmorphic anterior pituitary. A homozygous sequence variation in the consensus donor splice site of intron 1 (IVS1 + 2T > G) of the GHRHR gene was identified in both patients. Using in vitro transcription assay, we showed that this mutation results in abnormal splicing of GHRHR transcripts. In this report, which broadens the phenotype associated with GHRHR defects, we discuss the possible role of the GHRHR in the proper development of extrapituitary structures, through a mechanism that could be direct or secondary to severe GH deficiency. |
|||||
|---|---|---|---|---|---|---|
| MeSH term(s) | Alternative Splicing ; Child ; DNA Mutational Analysis ; Dwarfism, Pituitary/drug therapy ; Dwarfism, Pituitary/genetics ; Dwarfism, Pituitary/pathology ; Female ; Growth Hormone/therapeutic use ; Humans ; Magnetic Resonance Imaging ; Male ; Mutation ; Pedigree ; Phenotype ; RNA Splice Sites/genetics ; Receptors, Neuropeptide/genetics ; Receptors, Neuropeptide/physiology ; Receptors, Pituitary Hormone-Regulating Hormone/genetics ; Receptors, Pituitary Hormone-Regulating Hormone/physiology ; Reverse Transcriptase Polymerase Chain Reaction | |||||
| Chemical Substances | RNA Splice Sites ; Receptors, Neuropeptide ; Receptors, Pituitary Hormone-Regulating Hormone ; Growth Hormone (9002-72-6) ; somatotropin releasing hormone receptor (F8L0ODC9D7) | |||||
| Language | English | |||||
| Publishing date | 2008-02-20 | |||||
| Publishing country | England | |||||
| Document type | Case Reports ; Journal Article ; Research Support, Non-U.S. Gov't | |||||
| ZDB-ID | 1283676-x | |||||
| ISSN | 1076-1551 | |||||
| ISSN | 1076-1551 | |||||
| DOI | 10.2119/2007-00128.Hilal | |||||
| Shelf mark |
|
|||||
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
| Zs.A 4456: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.