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Article ; Online: Development of the Refugees and Asylum Seekers Occupational Satisfaction (RASOS) Assessment Tool.

Psychouli, Pavlina / Louta, Ioulia / Christodoulou, Constantina

International journal of environmental research and public health

2023 Volume 20, Issue 19

Abstract: The objective of this study was to develop an occupation-centered and client-centered assessment tool for refugees and asylum seekers. A preliminary tool outline was produced based on a literature review, while considering previous published tools' ... ...

Abstract	The objective of this study was to develop an occupation-centered and client-centered assessment tool for refugees and asylum seekers. A preliminary tool outline was produced based on a literature review, while considering previous published tools' strengths and limitations. A qualitative study was undertaken via focus groups to improve on the tool's design and adequacy for its purpose, resulting in the creation of a pilot version of the tool. Convenience sampling included 8 Greek and Cypriot professional and student occupational therapists with experience in the field, 8 international expert occupational therapists, 4 laypeople, 4 humanitarian professionals, and 5 refugees and asylum seekers. Basic qualitative content and thematic analysis led to topics regarding tool modifications that concerned categorization, formation/structure, wording, administration, and assessment scale. Corresponding tool revisions ensued. This study led to the development of the pilot version of the Refugees and Asylum Seekers Occupational Satisfaction (RASOS), which can also be used to identify underlying personal and environmental factors that contribute to self-perceived low satisfaction. A future quantitative study is required to establish the psychometric properties of the tool.
MeSH term(s)	Humans ; Refugees ; Health Services Accessibility ; Qualitative Research ; Focus Groups ; Occupational Therapists
Language	English
Publishing date	2023-09-25
Publishing country	Switzerland
Document type	Review ; Journal Article
ZDB-ID	2175195-X
ISSN	1660-4601 ; 1661-7827
ISSN (online)	1660-4601
ISSN	1661-7827
DOI	10.3390/ijerph20196826
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Article: The Utilization of an Aloe Vera Rind By-Product: Deep Eutectic Solvents as Eco-Friendly and Recyclable Extraction Media of Polyphenolic Compounds.

Ioannou, Georgia D / Ioannou, Katerina A / Christou, Atalanti / Stavrou, Ioannis J / Kapnissi-Christodoulou, Constantina P

Antioxidants (Basel, Switzerland)

2024 Volume 13, Issue 2

Abstract: In this study, an optimized environmentally friendly procedure was employed to enhance the sustainable utilization of phenolic antioxidants derived from aloe vera rind by-products. The procedure involved the application of ultrasound-assisted extraction ( ...

Abstract	In this study, an optimized environmentally friendly procedure was employed to enhance the sustainable utilization of phenolic antioxidants derived from aloe vera rind by-products. The procedure involved the application of ultrasound-assisted extraction (UAE) in combination with deep eutectic solvents (DESs). Eleven different DESs and three conventional solvents were employed as extraction media for polyphenolic compounds. Choline chloride-citric acid (ChCl-CA) was selected as the most suitable extractant, considering its extraction efficiency in relation to the total phenolic content. The operating conditions of UAE were optimized and modeled by the use of response surface methodology in order to maximize the yield of total phenolics and antioxidant capacity. The optimal operational parameters for the UAE procedure were determined to be 16.5 min, 74% (
Language	English
Publishing date	2024-01-26
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704216-9
ISSN	2076-3921
ISSN	2076-3921
DOI	10.3390/antiox13020162
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Article ; Online: Phenolic Profile, Antioxidant Activity, and Chemometric Classification of Carob Pulp and Products.

Ioannou, Georgia D / Savva, Ioanna K / Christou, Atalanti / Stavrou, Ioannis J / Kapnissi-Christodoulou, Constantina P

Molecules (Basel, Switzerland)

2023 Volume 28, Issue 5

Abstract: In recent years, carob and its derived products have gained wide attention due to their health-promoting effects, which are mainly attributed to their phenolic compounds. Carob samples (carob pulps, powders, and syrups) were analyzed to investigate their ...

Abstract	In recent years, carob and its derived products have gained wide attention due to their health-promoting effects, which are mainly attributed to their phenolic compounds. Carob samples (carob pulps, powders, and syrups) were analyzed to investigate their phenolic profile using high-performance liquid chromatography (HPLC), with gallic acid and rutin being the most abundant compounds. Moreover, the antioxidant capacity and total phenolic content of the samples were estimated through DPPH (IC
MeSH term(s)	Antioxidants/chemistry ; Flavonoids/chemistry ; Chemometrics ; Phenols/chemistry ; Fabaceae/chemistry
Chemical Substances	Antioxidants ; Flavonoids ; locust bean gum (V4716MY704) ; Phenols
Language	English
Publishing date	2023-02-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules28052269
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Article ; Online: Continuous and pulsed ultrasound-assisted extraction of carob's antioxidants: Processing parameters optimization and identification of polyphenolic composition.

Christou, Atalanti / Stavrou, Ioannis J / Kapnissi-Christodoulou, Constantina P

Ultrasonics sonochemistry

2021 Volume 76, Page(s) 105630

Abstract: Polyphenols in carobs have recently attracted great attention due to their wide range of biological and health promoting effects. A comprehensive study was conducted to find an optimum method for the extraction, purification and characterization of these ...

Abstract	Polyphenols in carobs have recently attracted great attention due to their wide range of biological and health promoting effects. A comprehensive study was conducted to find an optimum method for the extraction, purification and characterization of these valuable bioactive substances. Under this framework, the ultrasound-assisted extraction (UAE) of polyphenols from carob pulp was optimized by the maximization of the yield in total phenolics using response surface methodology. In particular, the effects of solid-solvent ratio, solvent concentration, extraction time, sonication amplitude, and sonication mode were investigated and optimized using a complete experimental design. In comparison to conventional extraction techniques, UAE offered a higher yield of antioxidants and a shorter processing time. Solid-phase extraction was evaluated as a clean-up strategy prior to the electrophoretic analysis of extracts. The results from the analysis of real samples revealed the predominance of gallic acid and highlighted the great influence of the ripening stage on carobs composition.
MeSH term(s)	Antioxidants/chemistry ; Antioxidants/isolation & purification ; Chemical Fractionation/methods ; Galactans/chemistry ; Mannans/chemistry ; Plant Gums/chemistry ; Polyphenols/analysis ; Solvents/chemistry ; Ultrasonic Waves
Chemical Substances	Antioxidants ; Galactans ; Mannans ; Plant Gums ; Polyphenols ; Solvents ; locust bean gum (V4716MY704)
Language	English
Publishing date	2021-06-12
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1208333-1
ISSN	1873-2828 ; 1350-4177
ISSN (online)	1873-2828
ISSN	1350-4177
DOI	10.1016/j.ultsonch.2021.105630
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Article ; Online: Novel supramolecular deep eutectic solvent (SUPRADES) as a sole chiral selector in capillary electrophoresis for the enantiomeric separation of fluorine-substituted amphetamine analogs.

Ioannou, Katerina A / Ioannou, Georgia D / Christou, Atalanti / Schmid, Martin G / Stavrou, Ioannis J / Kapnissi-Christodoulou, Constantina P

Journal of chromatography. A

2024 Volume 1715, Page(s) 464628

Abstract: In this study, a novel supramolecular deep eutectic solvent consisting of sulfated-β-CD and citric acid (S-β-CD-CA) is reported for the first time. This innovative system was evaluated as a sole chiral selector in capillary electrophoresis for the ... ...

Abstract	In this study, a novel supramolecular deep eutectic solvent consisting of sulfated-β-CD and citric acid (S-β-CD-CA) is reported for the first time. This innovative system was evaluated as a sole chiral selector in capillary electrophoresis for the enantioseparation of six fluorine-substituted amphetamine analogs, yielding remarkable outcomes. Baseline separations of all amphetamine analogs under study were achieved in less than 21.00 min using the S-β-CD-CA as the chiral selector. It was observed that the addition of 0.050 % v/v S-β-CD-CA into the background electrolyte resulted in the baseline separation of five out of the six fluorine-substituted amphetamine analogs, while in the case of the para-substituted amphetamine analog, 4-fluoramphetamine (4-FA), a higher percentage (0.15 % v/v) was required to achieve baseline enantioseparation. These findings emphasized the potential of this new supramolecular system in providing a class of solvents with promising chiral recognition properties.
MeSH term(s)	beta-Cyclodextrins ; Amphetamine ; Deep Eutectic Solvents ; Fluorine ; Solvents ; Electrophoresis, Capillary/methods ; Sulfates ; Stereoisomerism
Chemical Substances	beta-Cyclodextrins ; Amphetamine (CK833KGX7E) ; Deep Eutectic Solvents ; Fluorine (284SYP0193) ; Solvents ; Sulfates
Language	English
Publishing date	2024-01-03
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1171488-8
ISSN	1873-3778 ; 0021-9673
ISSN (online)	1873-3778
ISSN	0021-9673
DOI	10.1016/j.chroma.2024.464628
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Article ; Online: Comparison of cyclofructan-, cyclodextrin-, and polysaccharide-based chiral stationary phases for the separation of pharmaceuticals.

Agathokleous, Eliana A / Stavrou, Ioannis J / Kapnissi-Christodoulou, Constantina

Analytical and bioanalytical chemistry

2021 Volume 414, Issue 3, Page(s) 1323–1333

Abstract: In this study, cyclofructan (CF)-, cyclodextrin (CD)-, and polysaccharide-based chiral stationary phases (CSPs) were exploited in high-performance liquid chromatography (HPLC) for the chiral separations of different clinically and pharmaceutically ... ...

Abstract	In this study, cyclofructan (CF)-, cyclodextrin (CD)-, and polysaccharide-based chiral stationary phases (CSPs) were exploited in high-performance liquid chromatography (HPLC) for the chiral separations of different clinically and pharmaceutically important compounds. In particular, R-naphthylethyl carbamate CF6 (RN-CF6), 3,5-dimethylphenyl carbamate CF7 (DMP-CF7), neutral beta cyclodextrin (β-CD), 3,5-dimethylphenyl carbamate β-CD (DMP-β-CD), and cellulose tris-(3,5-dimethylphenylcarbamate) (Cellulose-Tris DMP) columns were utilized under isocratic elution. The performance of these CSPs as chiral separation media was evaluated by use of nine analytes: acidic, basic, and amphiprotic. A possible correlation between the functional groups of these analytes and the chiral-recognition ability of each chiral column was also examined. The enantioseparations were optimized by varying different parameters, such as mobile phase additives, column temperature, and flow rate. Finally, a comparison was made between all CSPs, and it was expressed in terms of resolution (R
MeSH term(s)	Chromatography, High Pressure Liquid/methods ; Cyclodextrins/chemistry ; Fructans/chemistry ; Pharmaceutical Preparations/isolation & purification ; Polysaccharides/chemistry ; Stereoisomerism
Chemical Substances	Cyclodextrins ; Fructans ; Pharmaceutical Preparations ; Polysaccharides ; cyclofructan 6
Language	English
Publishing date	2021-11-15
Publishing country	Germany
Document type	Comparative Study ; Journal Article
ZDB-ID	201093-8
ISSN	1618-2650 ; 0016-1152 ; 0372-7920
ISSN (online)	1618-2650
ISSN	0016-1152 ; 0372-7920
DOI	10.1007/s00216-021-03754-1
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Article ; Online: Combined use of β-cyclodextrin and ionic liquid as electrolyte additives in EKC for separation and determination of carob's phenolics-A study of the synergistic effect.

Christou, Atalanti / Stavrou, Ioannis J / Kapnissi-Christodoulou, Constantina P

Electrophoresis

2021 Volume 42, Issue 19, Page(s) 1945–1955

Abstract: In this work, a simple, reliable, and fast capillary electrophoretic method was developed and validated for the simultaneous determination of 12 polyphenolic compounds, the most frequently found in carob's pulp and seeds. The present work deals with the ... ...

Abstract	In this work, a simple, reliable, and fast capillary electrophoretic method was developed and validated for the simultaneous determination of 12 polyphenolic compounds, the most frequently found in carob's pulp and seeds. The present work deals with the development of a novel dual electrophoretic system based on the combined use of β-CD and ionic liquid (IL) as buffer additives. A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC
MeSH term(s)	Electrolytes ; Fabaceae ; Galactans ; Ionic Liquids ; Mannans ; Phenols ; Plant Gums ; beta-Cyclodextrins
Chemical Substances	Electrolytes ; Galactans ; Ionic Liquids ; Mannans ; Phenols ; Plant Gums ; beta-Cyclodextrins ; locust bean gum (V4716MY704)
Language	English
Publishing date	2021-07-19
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	619001-7
ISSN	1522-2683 ; 0173-0835
ISSN (online)	1522-2683
ISSN	0173-0835
DOI	10.1002/elps.202100085
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Article ; Online: The potential of the use of deep eutectic solvents and amino acid-based ionic liquids to enhance the chiral discrimination ability of different chiral selectors in capillary electrophoresis

Ioannou, Katerina A. / Ioannou, Georgia D. / Christou, Atalanti / Stavrou, Ioannis J. / Schmid, Martin G. / Kapnissi-Christodoulou, Constantina P.

Journal of Chromatography A. 2023 Aug., v. 1705 p.464152-

2023

Abstract: The effect of the combined use of amino acid-based ionic liquids (AAILs) and deep eutectic solvents (DESs) with either cyclodextrin- (CD) or cyclofructan- (CF) based chiral selectors for the chiral separation of amphetamine derivatives was investigated ... ...

Abstract	The effect of the combined use of amino acid-based ionic liquids (AAILs) and deep eutectic solvents (DESs) with either cyclodextrin- (CD) or cyclofructan- (CF) based chiral selectors for the chiral separation of amphetamine derivatives was investigated in the present study. A non-significant improvement in enantiomeric separation of target analytes was observed when AAILs were combined with either CF or CD. On the other side, a markedly improved chiral separation of enantiomers was obtained using the dual carboxymethyl-β-cyclodextrin/DES system, highlighting the existence of a synergistic effect. After the addition of 0.5% v/v of choline chloride-ethylene glycol, the resolution of the enantiomers of amphetamine, methamphetamine and 3-fluorethamphetamine, increased from 1.4, 1.1, 1.0 to 1.8, 1.8, and 1.5 min, and the analysis times increased from 19.54, 20.48, 18.71 to 35.71, 35.78 and 32.90 min, respectively. This was not the case for the CF/DES dual system, in which the separation of amphetamines worsened, indicating an antagonistic effect. In conclusion, DESs are a very promising additive in capillary electrophoresis that can improve the separation of chiral molecules in combination with CDs but not CFs.
Keywords	amphetamine ; capillary electrophoresis ; chemical species ; choline ; chromatography ; enantiomers ; methamphetamines ; synergism ; Amino acid-based ionic liquids ; Deep eutectic solvents ; Cyclodextrin ; Cyclofructan ; Amphetamine derivatives
Language	English
Dates of publication	2023-08
Publishing place	Elsevier B.V.
Document type	Article ; Online
ZDB-ID	218139-3
ISSN	0021-9673 ; 0378-4355 ; 0376-737X
ISSN	0021-9673 ; 0378-4355 ; 0376-737X
DOI	10.1016/j.chroma.2023.464152
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Article: Comparison of cyclofructan-, cyclodextrin-, and polysaccharide-based chiral stationary phases for the separation of pharmaceuticals

Agathokleous, Eliana A. / Stavrou, Ioannis J. / Kapnissi-Christodoulou, Constantina

Analytical and bioanalytical chemistry. 2022 Jan., v. 414, no. 3

2022

Abstract	In this study, cyclofructan (CF)-, cyclodextrin (CD)-, and polysaccharide-based chiral stationary phases (CSPs) were exploited in high-performance liquid chromatography (HPLC) for the chiral separations of different clinically and pharmaceutically important compounds. In particular, R-naphthylethyl carbamate CF6 (RN–CF6), 3,5-dimethylphenyl carbamate CF7 (DMP–CF7), neutral beta cyclodextrin (β–CD), 3,5-dimethylphenyl carbamate β–CD (DMP–β–CD), and cellulose tris-(3,5-dimethylphenylcarbamate) (Cellulose–Tris DMP) columns were utilized under isocratic elution. The performance of these CSPs as chiral separation media was evaluated by use of nine analytes: acidic, basic, and amphiprotic. A possible correlation between the functional groups of these analytes and the chiral-recognition ability of each chiral column was also examined. The enantioseparations were optimized by varying different parameters, such as mobile phase additives, column temperature, and flow rate. Finally, a comparison was made between all CSPs, and it was expressed in terms of resolution (RS), efficiency (N), selectivity (α), retention factors (k₁′, k₂′) and analysis time (tR₁, tR₂). It was observed that RN–CF6 was the most suitable and efficient CSP for the chiral separation of various types of analytes, including acids, primary and tertiary amines, alcohols, and many neutral compounds. It was the only CSP that provided baseline enantioseparation of thyroxine (RS = 1.6) and cetirizine (RS = 2.0).
Keywords	analytical chemistry ; beta-cyclodextrin ; cellulose ; chemical species ; drugs ; high performance liquid chromatography ; temperature ; thyroxine
Language	English
Dates of publication	2022-01
Size	p. 1323-1333.
Publishing place	Springer Berlin Heidelberg
Document type	Article
ISSN	1618-2642
DOI	10.1007/s00216-021-03754-1
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Article: Evaluation of different extraction procedures for the quantification of seven cannabinoids in cannabis-based edibles by the use of LC-MS

Christodoulou, Marios C. / Christou, Atalanti / Stavrou, Ioannis J. / Kapnissi-Christodoulou, Constantina P.

Journal of food composition and analysis. 2022 Sept. 16,

2022

Abstract: Although still illegal in many countries, food products containing cannabis or marijuana extracts have become very popular in recent years. In the present study, an LC-MS method was developed for the quantitative analysis of seven cannabinoids in various ...

Abstract	Although still illegal in many countries, food products containing cannabis or marijuana extracts have become very popular in recent years. In the present study, an LC-MS method was developed for the quantitative analysis of seven cannabinoids in various solid and liquid cannabis-based goods. The proposed analytical approach demonstrated satisfactory performance characteristics in terms of linearity (R²≥0.995), accuracy (recovery: 70.0-110%), precision (intraday RSD: 0.950-6.03%, interday RSD: 1.02-6.94%), sensitivity (LOD≤2.19ng/mL, LOQ≤6.59ng/mL) and carry-over effect (average carryover signals ≤3.90%). Solid-phase extraction (SPE), and ultrasound-assisted extraction (UAE) were utilized for the extraction of the analytes from liquid cannabis edibles (beer and energy drink), while Soxhlet and ultrasound-assisted extraction (UAE) were used for solid products (chocolates, hemp seeds, hemp tea). Infusion and decoction processes were followed for cannabis hemp tea and roasted coffee, respectively. UAE provided higher extraction efficiencies for cannabis-based edibles in solid form, while infused-cannabis beverages were extracted more efficiently using the SPE procedure. Cannabidiol (CBD) and cannabigerol (CBG) were the most detectable cannabinoids in all examined samples. Significantly high levels of cannabinoids were detected in cannabis tea extract prepared by the UAE procedure (total cannabinoids: 5440μg/g). According to the suppliers, all examined samples were supposed to be free of Δ⁹-tetrahydrocannabinol (Δ⁹-THC). However, five products were found to contain considerable amounts of this compound (0.600-180μg/g). Only in the case of cannabis beer, cannabis roasted coffee, and cannabis energy drink, Δ⁹-THC was not detected.
Keywords	Cannabis sativa ; analytical methods ; beers ; cannabidiol ; chemical species ; energy drinks ; food composition ; hemp ; liquids ; quantitative analysis ; solid phase extraction ; tea ; ultrasonic treatment
Language	English
Dates of publication	2022-0916
Publishing place	Elsevier Inc.
Document type	Article
Note	Pre-press version
ZDB-ID	743572-1
ISSN	0889-1575 ; 1096-0481
ISSN	0889-1575 ; 1096-0481
DOI	10.1016/j.jfca.2022.104915
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