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  1. Article ; Online: Optimization of Ultrasonic Flavonoid Extraction from Saussurea involucrate, and the Ability of Flavonoids to Block Melanin Deposition in Human Melanocytes

    Chun-Yan Dai / Pei-Ran Liao / Ming-Zhuo Zhao / Chao Gong / Yue Dang / Yuan Qu / Li-Sha Qiu

    Molecules, Vol 25, Iss 2, p

    2020  Volume 313

    Abstract: 1) Background: Flavonoids are the primary medicinal ingredient of Saussurea involucrate , which have significant antioxidant capacity. Optimizing the extraction of Saussurea involucrate flavonoids (SIFs) and exploring the ability to block melanin ... ...

    Abstract (1) Background: Flavonoids are the primary medicinal ingredient of Saussurea involucrate , which have significant antioxidant capacity. Optimizing the extraction of Saussurea involucrate flavonoids (SIFs) and exploring the ability to block melanin deposition caused by reactive oxygen can greatly promote the development of S. involucrate whitening products. (2) Methods: Ultrasonic extraction process was optimized using the Box−Behnken design (BBD) and response surface methodology (RSM). Then, the effect of SIFs on antioxidant activity and anti-deposition of melanin, and genes related to the melanin synthesis are studied. (3) Results: The optimal extraction procedures are as follows: the extraction time, ethanol content, and solvent ratio ( v / w ) are 64 min, 54%, and 54:1, respectively. The reducing activity and scavenging rates of 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide anion, hydroxyl radical, and ABTS + were promoted as more S. involucrate flavonoid extract was added. The SIFs extract induced a decrease in the melanin synthesis by inhibiting the human melanoma A375 cell tyrosinase activity. SIFs also depress expression of melanin synthesis related genes. (4) Conclusions: the highest SIFs content was obtained by using 54% ethanol and 54:1 solvent ratio ( v / w ) for 64 min. The extract of SIFs exhibited good ability of antioxidant and anti-deposition of melanin in human melanocytes.
    Keywords saussurea involucrate ; ultrasonic extraction process ; response surface ; antioxidant ; human melanoma a375 cells ; Organic chemistry ; QD241-441
    Subject code 660
    Language English
    Publishing date 2020-01-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Optimization of Flavonoids Extraction Process in Panax notoginseng Stem Leaf and a Study of Antioxidant Activity and Its Effects on Mouse Melanoma B16 Cells

    Chun-Yan Dai / Peng-Fei Liu / Pei-Ran Liao / Yuan Qu / Cheng-Xiao Wang / Ye Yang / Xiu-Ming Cui

    Molecules, Vol 23, Iss 9, p

    2018  Volume 2219

    Abstract: The Panax notoginseng (P. notoginseng) stem leaf is rich in flavonoids. However, because of a lack of research on the flavonoid extraction process and functional development of P. notoginseng stem leaf, these parts are discarded as agricultural wastes. ... ...

    Abstract The Panax notoginseng (P. notoginseng) stem leaf is rich in flavonoids. However, because of a lack of research on the flavonoid extraction process and functional development of P. notoginseng stem leaf, these parts are discarded as agricultural wastes. Therefore, in this study, we intend to optimize the extraction process and develop the skin-whitening functions of P. notoginseng stem leaf extracts. The extraction process of the stem and leaf of P. notoginseng flavonoid (SLPF) is optimized based on the Box–Behnken design (BBD) and the response surface methodology (RSM). The optimum extraction conditions of the SLPF are as follows: the extraction time, the ethanol concentration, the sodium dodecyl sulfate (SDS) content and the liquid material ratio (v/w, which are 52 min, 48.7%, 1.9%, and 20:1, respectively. Under the optimal extraction conditions, the average total SLPF content is 2.10%. The antioxidant activity and anti-deposition of melanin of mouse B16 cells of P. notoginseng stem leaf extracts are studied. The results indicate that the EC50 values of reducing activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activities, the superoxide anion removal ability, and the 2,2-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) free radical removal ability are 7.212, 2.893, 2.949, and 0.855 mg/mL, respectively. The extracts IC50 values of the tyrosinase and melanin synthesis are 0.045 and 0.046 mg/mL, respectively. Therefore, the optimal processing technology for the SLPF obtained in this study not only increases its utilization rate, but also decreases material costs. The extracts from the P. notoginseng stem leaf may be developed as food or beauty products.
    Keywords antioxidant ; flavonoids ; mouse melanoma B16 cells ; Panax notoginseng ; surfactant ; Organic chemistry ; QD241-441
    Subject code 660
    Language English
    Publishing date 2018-09-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: A new triterpenoid glucoside from

    Chen, Guang-Ying / Zhang, Bin / Zhao, Ting / Nidhal, Najat / Jia-Li, Wang / Zhou, Xue-Ming / Chun-Yan, Dai

    Natural product research

    2019  Volume 34, Issue 13, Page(s) 1874–1878

    Abstract: A new triterpenoid glucoside, leuctriterpencoside ( ...

    Abstract A new triterpenoid glucoside, leuctriterpencoside (
    MeSH term(s) Glucosides/chemistry ; Glucosides/isolation & purification ; Glycoside Hydrolase Inhibitors/isolation & purification ; Glycoside Hydrolase Inhibitors/pharmacology ; Lamiaceae/chemistry ; Lamiaceae/metabolism ; Molecular Structure ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Triterpenes/chemistry ; Triterpenes/isolation & purification ; alpha-Glucosidases/drug effects ; alpha-Glucosidases/metabolism
    Chemical Substances Glucosides ; Glycoside Hydrolase Inhibitors ; Plant Extracts ; Triterpenes ; alpha-Glucosidases (EC 3.2.1.20)
    Language English
    Publishing date 2019-01-28
    Publishing country England
    Document type Journal Article
    ZDB-ID 2185747-7
    ISSN 1478-6427 ; 1478-6419
    ISSN (online) 1478-6427
    ISSN 1478-6419
    DOI 10.1080/14786419.2019.1566820
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: On the Solvability of Superlinear and Nonhomogeneous Quasilinear Equations

    Gao Jia / Qing Zhao / Chun-yan Dai

    Boundary Value Problems, Vol

    2009  Volume 2009

    Abstract: Using Mountain Pass Lemma, we obtain the existence of nontrivial weak solutions for a class of superlinear and nonhomogeneous quasilinear equations. The key factor in this paper is to use the new idea of near p-homogeneity in conjunction with variational ...

    Abstract Using Mountain Pass Lemma, we obtain the existence of nontrivial weak solutions for a class of superlinear and nonhomogeneous quasilinear equations. The key factor in this paper is to use the new idea of near p-homogeneity in conjunction with variational techniques to obtain a new multiplicity result for a vast set of nonlinear equations, such as the mean curvature equation and so on.
    Keywords Mathematics ; QA1-939 ; Science ; Q ; DOAJ:Mathematics ; DOAJ:Mathematics and Statistics
    Language English
    Publishing date 2009-01-01T00:00:00Z
    Publisher Springer
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article: A new isoflavone from the roots of Ficus auriculata

    Qi, Cui-Cui / Yan-Hui Fu / Wen-Hao Chen / Guang-Ying Chen / Chun-Yan Dai / Xiao-Ping Song / Chang-Ri Han

    Natural product research. 2018 Jan. 2, v. 32, no. 1

    2018  

    Abstract: A new isoflavone, 5,7,4′-trihydroxy-3′-hydroxymethylisoflavone (1), together with three known isoflavones, 3′-formyl-5,4′-dihydroxy-7-methoxyisoflavone (2), ficuisoflavone (3) and alpinumisoflavone (4), were isolated from the roots of Ficus auriculata. ... ...

    Abstract A new isoflavone, 5,7,4′-trihydroxy-3′-hydroxymethylisoflavone (1), together with three known isoflavones, 3′-formyl-5,4′-dihydroxy-7-methoxyisoflavone (2), ficuisoflavone (3) and alpinumisoflavone (4), were isolated from the roots of Ficus auriculata. Among them, 1 is a rare isoflavone containing 16 carbon atoms on the carbon skeleton. The structure of 1 was elucidated by extensive spectroscopic methods and the known compounds were identified by comparisons with data reported in the literature. All compounds were evaluated for their antibacterial activities against five terrestrial pathogenic bacteria in vitro. Compounds 1–4 showed significant antibacterial activities against various terrestrial pathogenic bacteria with MIC values ranging from 1.30 to 39.93 μM.
    Keywords Ficus ; antibacterial properties ; bacteria ; carbon ; isoflavones ; minimum inhibitory concentration ; roots ; spectroscopy
    Language English
    Dates of publication 2018-0102
    Size p. 43-47.
    Publishing place Taylor & Francis
    Document type Article
    ZDB-ID 2185747-7
    ISSN 1478-6427 ; 1478-6419
    ISSN (online) 1478-6427
    ISSN 1478-6419
    DOI 10.1080/14786419.2017.1329728
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: iTRAQ-based proteomics screen for potential regulators of wheat (Triticum aestivum L.) root cell wall component response to Al stress

    Yang, Ye / Cai-Xia Li / Chun-Yan Dai / Hong Zeng / Hui-Lin Guan / Li Ma / Lu-Yao Chen / Nan Wu / Xi Mu / Xiu-Ming Cui / Ying Liu / Yun Zheng / Ze-Ping Yang

    Gene. 2018,

    2018  

    Abstract: The inhibition of Aluminum (Al)-induced root tip cell elongation is a major cause of plant root elongation suppression. The inhibition of root tip cell elongation is caused by a disruption of cell wall component metabolism, growth signaling, or cellular ... ...

    Abstract The inhibition of Aluminum (Al)-induced root tip cell elongation is a major cause of plant root elongation suppression. The inhibition of root tip cell elongation is caused by a disruption of cell wall component metabolism, growth signaling, or cellular damage. The aim of this study was to identify the proteins involved in the metabolism of the root cell wall components under Al stress in the Al-tolerant wheat (Triticum aestivum L.) cultivar ET8.Differentially expressed proteins of Al-tolerant wheat roots were screened via isobaric tags for relative and absolute quantification (iTRAQ). Furthermore, their expression changes were detected via RT-PCR analysis. The contents of major components of the cell wall and their changes in metabolic enzyme activities were also investigated.A total of 97 differentially expressed proteins from Al-tolerant wheat roots were screened and nine of these 97 proteins were root cell wall component related. The known nucleic acid sequences of proteins were 14-3-3 protein, the plasm membrane (PM) H+-ATPase, phospholipase D, peroxidase, and glycosyltransferase. For 14-3-3 protein, phospholipase D and peroxidase, the protein expression and mRNA expression were consistent with Al-treatment; however, for PM H+-ATPase and glycosyltransferase, the protein expression and mRNA expression were inconsistent under Al-stress. Furthermore, both cellulase activity and callase activity were down-regulated by Al stress, while the phenylalanineammonialyase (PAL), cinnamyl alcohol dehydrogenase (CAD), and peroxidase (POD) activities were up-regulated. Furthermore, the PM H+-ATPase activity was decreased in response to Al stress. In addition, the contents of callose, cellulose, lignin, and H2O2 varied significantly.The cell wall components, relative metabolism enzymes activity, and gene expression also changed followed by protein expression changed in response to Al stress. The results suggest that Al stress leads to marked variations in metabolic enzyme activity, carbohydrate content, followed by changes of root cell components in wheat roots.
    Keywords aluminum ; callose ; carbohydrate content ; cell growth ; cell wall components ; cellulose ; cinnamyl alcohol dehydrogenase ; cultivars ; endo-1,4-beta-glucanase ; enzyme activity ; gene expression ; glycosyltransferases ; H-transporting ATP synthase ; hydrogen peroxide ; lignin ; messenger RNA ; nucleotide sequences ; peroxidase ; phospholipase D ; protein synthesis ; proteins ; proteomics ; reverse transcriptase polymerase chain reaction ; root growth ; root tips ; Triticum aestivum ; wheat
    Language English
    Size p. .
    Publishing place Elsevier B.V.
    Document type Article
    Note Pre-press version
    ZDB-ID 391792-7
    ISSN 1879-0038 ; 0378-1119
    ISSN (online) 1879-0038
    ISSN 0378-1119
    DOI 10.1016/j.gene.2018.07.008
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: A new flavonol from the stem-bark of Premna fulva

    Guang-Ying Chen / Chun-Yan Dai / Tian-Shan Wang / Cai-Wu Jiang / Chang-Ri Han / Xiao-Ping Song

    ARKIVOC, Vol 2010, Iss 2, Pp 179-

    2010  Volume 185

    Keywords Organic chemistry ; QD241-441
    Language English
    Publishing date 2010-05-01T00:00:00Z
    Publisher Arkat USA, Inc.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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