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  1. Article ; Online: Causes and outcomes of implantable collamer lens explantation in patients with corneal endothelial cell loss.

    Yoon, Hye Yeon / Byun, Yong-Soo / Kim, Hyun Seung / Chung, So-Hyang

    Journal of cataract and refractive surgery

    2024  Volume 50, Issue 5, Page(s) 453–459

    Abstract: Purpose: To investigate the correlated factors of corneal endothelial cell density (ECD) loss and ECD change in implantable collamer lens (ICL) explantation patients.: Setting: Seoul St. Mary's Hospital, Seoul, South Korea.: Design: Retrospective ... ...

    Abstract Purpose: To investigate the correlated factors of corneal endothelial cell density (ECD) loss and ECD change in implantable collamer lens (ICL) explantation patients.
    Setting: Seoul St. Mary's Hospital, Seoul, South Korea.
    Design: Retrospective analysis.
    Methods: The study cohort consisted of 93 eyes from 50 patients who underwent ICL explantation. Correlation analysis was performed to assess the ocular parameters associated with ECD loss, while percentage of ECD change (ΔECD%) was monitored up to 6 months postoperatively. Receiver operating characteristic (ROC) curve was used to set cutoff values of ocular parameters to prevent ECD loss after explantation.
    Results: In multiple regression analysis, high vaulting, high vaulting/anterior chamber depth (ACD), low anterior chamber angle (ACA), and high iris pigmentations are the significant factors of ECD loss. At postoperative 6 months, 14 eyes (15.1%) had decrease (10.5% loss), 47 eyes (51.0%) were stationary, and 32 eyes (34.4%) had increase (12.7% gain) of ECD. The ROC curve analysis showed that vaulting had the highest area under the curve (AUC = 0.822), followed by vaulting/ACD (AUC = 0.821), ECD (AUC = 0.753), and ACA (AUC = 0.723) (all P < .01). Preoperative ECD showed a sensitivity of 77.6% and specificity of 86.7% in preventing ECD loss after explantation, with a cutoff value of 1722 cells/mm 2 , as determined by ROC curve analysis.
    Conclusions: Our study demonstrated that high vaulting is a significant factor in ECD loss among ICL-inserted patients. To prevent continuous ECD loss in ICL patients, close monitoring of ECD and making appropriate decisions regarding explantation may be necessary.
    Language English
    Publishing date 2024-04-18
    Publishing country United States
    Document type Journal Article
    ZDB-ID 632744-8
    ISSN 1873-4502 ; 0886-3350
    ISSN (online) 1873-4502
    ISSN 0886-3350
    DOI 10.1097/j.jcrs.0000000000001406
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  2. Article: Accuracy of Toric Intraocular Lens Calculators with Predicted and Measured Posterior Corneal Astigmatism Across Different Types of Astigmatism.

    Yang, Soonwon / Park, Jaehyun / Whang, Woong Joo / Byun, Yong-Soo / Kim, Hyun Seung / Chung, So-Hyang

    Ophthalmology and therapy

    2024  

    Abstract: Introduction: This study is a retrospective case series to compare the accuracy of the Barrett toric calculator using predicted posterior corneal astigmatism (PCA) and PCA measurements using swept-source optical coherence tomography (SS-OCT) and a ... ...

    Abstract Introduction: This study is a retrospective case series to compare the accuracy of the Barrett toric calculator using predicted posterior corneal astigmatism (PCA) and PCA measurements using swept-source optical coherence tomography (SS-OCT) and a Scheimpflug camera. This evaluation was conducted across different types of anterior and posterior astigmatism.
    Methods: A total of 146 eyes from 146 patients implanted with toric intraocular lenses were included. Mean absolute prediction error, standard deviation of prediction error, and the percentage of eyes with prediction errors within ±0.50 diopters (D) were calculated using vector analysis. Biometric measurements were conducted using the IOLMaster 700 and Pentacam HR. A subgroup analysis was conducted based on the orientation of both anterior and posterior corneal astigmatism.
    Results: The Barrett toric calculator with predicted PCA yielded the best results, with 78.1% having a prediction error ≤ 0.50 D, which was a significantly higher percentage than the Barrett formula with the two versions of measured PCA (P < 0.05). In the subgroup with a horizontally steep meridian PCA using the IOLMaster 700, the Barrett formula with predicted PCA yielded the best results, with 78.3% of cases having a prediction error of less than 0.5 D. This percentage was significantly higher than the other two measured PCA subgroups (P < 0.05).
    Conclusion: The Barrett toric formula with predicted PCA demonstrated a statistically significantly higher proportion of cases with a prediction error ≤ 0.5 D compared to the two measured PCA formulas (from the IOLMaster 700 or Pentacam). This trend persisted even when the posterior corneal astigmatism was horizontally steep.
    Language English
    Publishing date 2024-04-06
    Publishing country England
    Document type Journal Article
    ISSN 2193-8245
    ISSN 2193-8245
    DOI 10.1007/s40123-024-00931-0
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  3. Article ; Online: Efficacy and safety of platelet-rich plasma and autologous-serum eye drops for dry eye in primary Sjögren's syndrome: a randomized trial.

    Kang, Min-Ji / Lee, Jee Hye / Hwang, Jehyung / Chung, So-Hyang

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 19279

    Abstract: We compared the efficacy and safety of autologous-serum (AS) and platelet-rich plasma (PRP) eye drops for dry eye (DE) treatment in primary Sjögren's syndrome (SS). This prospective, randomized, double-blinded clinical study included patients diagnosed ... ...

    Abstract We compared the efficacy and safety of autologous-serum (AS) and platelet-rich plasma (PRP) eye drops for dry eye (DE) treatment in primary Sjögren's syndrome (SS). This prospective, randomized, double-blinded clinical study included patients diagnosed with primary SS DE. Thirty-eight participants were randomly assigned to the AS or PRP groups. Corneal and conjunctival staining scores, Schirmer I test, tear film break-up time (TBUT), and ocular surface disease index (OSDI) scores were evaluated at 4 and 12 weeks. Conjunctival impression cytology (CIC) metaplasia grade and goblet cell density grade at 12 weeks were compared with those at baseline. Corneal and conjunctival staining scores and TBUT significantly improved at 4 and 12 weeks in both groups (all p < 0.005). No significant difference between the AS and PRP groups was observed at 4 and 12 weeks. The Schirmer I values, OSDI scores, CIC metaplasia grade, and goblet cell density grade did not significantly change at 4 and 12 weeks in either group. Both AS and PRP eye drops are effective for primary SS DE without a significant difference. Considering that the preparation time of PRP is shorter than that of AS, PRP can be a good alternative treatment for primary SS DE.
    MeSH term(s) Humans ; Dry Eye Syndromes/drug therapy ; Metaplasia ; Ophthalmic Solutions/adverse effects ; Platelet-Rich Plasma ; Prospective Studies ; Sjogren's Syndrome/complications ; Sjogren's Syndrome/therapy ; Sjogren's Syndrome/diagnosis ; Tears
    Chemical Substances Ophthalmic Solutions
    Language English
    Publishing date 2023-11-07
    Publishing country England
    Document type Journal Article ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-46671-2
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  4. Article ; Online: Comparative Analysis of the Clinical Outcomes of Mix-and-Match Implantation of an Extended Depth-of-Focus and a Diffractive Bifocal Intraocular Lens.

    Kim, Seonjoo / Yi, Rowoon / Chung, So-Hyang

    Eye & contact lens

    2022  Volume 48, Issue 6, Page(s) 261–266

    Abstract: Objectives: To evaluate and compare the clinical outcomes of the extended depth-of-focus intraocular lens (EDOF-IOL) and the diffractive bifocal IOL.: Methods: Fifty-six eyes of 28 patients underwent cataract surgery with implantation of an EDOF-IOL ( ...

    Abstract Objectives: To evaluate and compare the clinical outcomes of the extended depth-of-focus intraocular lens (EDOF-IOL) and the diffractive bifocal IOL.
    Methods: Fifty-six eyes of 28 patients underwent cataract surgery with implantation of an EDOF-IOL (TECNIS Symfony, ZXR00) in their dominant eye and a diffractive bifocal IOL (+3.25 D add TECNIS ZLB00) in their nondominant eye. Monocular and binocular uncorrected distance, intermediate and near visual acuity, defocus curves, contrast sensitivity, patient satisfaction score (0-5), and spectacle dependence at near vision were evaluated at 3 months after the surgery.
    Results: Postoperative binocular uncorrected distant visual acuity was 0.009±0.031 logMAR, uncorrected intermediate visual acuity was 0.090±0.158 logMAR, and uncorrected near visual acuity was 0.099±0.068 logMAR. The monocular defocus curve demonstrated better performance at intermediate distance in eyes with an EDOF-IOL and better performance at near distance in eyes with a diffractive bifocal IOL. An EDOF-IOL exhibited better outcomes in contrast sensitivity compared with a diffractive bifocal IOL. Three patients (10.7%) required glasses for near vision.
    Conclusion: Mix-and-match implantation of a diffractive bifocal IOL with an EDOF-IOL provides an excellent range of vision, and high levels of contrast sensitivity and patient satisfaction.
    MeSH term(s) Eyeglasses ; Humans ; Lens Implantation, Intraocular ; Lenses, Intraocular ; Multifocal Intraocular Lenses ; Patient Satisfaction ; Phacoemulsification ; Prospective Studies ; Prosthesis Design ; Refraction, Ocular ; Vision, Binocular
    Language English
    Publishing date 2022-03-24
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2103049-2
    ISSN 1542-233X ; 1542-2321
    ISSN (online) 1542-233X
    ISSN 1542-2321
    DOI 10.1097/ICL.0000000000000887
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  5. Article ; Online: Prevalence of Macular Abnormalities Identified Only on Optical Coherence Tomography in Korean Patients Scheduled for Cataract Surgery.

    Hong, Seo-Yeon / Kim, Seonjoo / Chung, So-Hyang

    Korean journal of ophthalmology : KJO

    2021  Volume 35, Issue 2, Page(s) 153–158

    Abstract: Purpose: To assess the prevalence of macular abnormalities identified only on macular optical coherence tomography (OCT) which were not suspected by biomicroscopic fundus examination, and examine the clinical outcome of patients with these macular ... ...

    Abstract Purpose: To assess the prevalence of macular abnormalities identified only on macular optical coherence tomography (OCT) which were not suspected by biomicroscopic fundus examination, and examine the clinical outcome of patients with these macular abnormalities during preoperative evaluation for cataract surgery in a large series of Korean patients.<br/>.
    Methods: Macular OCT was performed on patients scheduled for routine cataract surgery by the same physician at Seoul St. Mary's Hospital, between June 2018 and November 2019. The patients' medical records were reviewed retrospectively to obtain demographic data and the results of preoperative evaluation before cataract surgery. Patients were divided into two groups based on the preoperative macular OCT.
    Results: normal and abnormal OCT groups.
    Results: Nine hundred eighty-seven eyes (698 patients) were included in this study. Macular OCT identified abnormalities in 44 eyes (4.5%) of 35 patients (5.0%). Twenty-one eyes (2.1%) had age-related macular degeneration, 20 eyes (2.0%) had epiretinal membrane, and three eyes (0.3%) had lamellar hole. Patients with macular abnormalities identified on macular OCT had a statistically significant higher mean age than those who had normal OCT findings (p < 0.001). Best-corrected visual acuity was worse in patients with abnormal macular OCT after cataract surgery (p = 0.048).
    Conclusions: In the preoperative evaluation for cataract surgery in Korean patients, one in every 20 patients had macular abnormalities identified only on macular OCT in spite of unremarkable macular findings on biomicroscopic funduscopy. Age was significantly higher in patients with abnormal macular OCT findings. Thus, inclusion of macular OCT examination in preoperative screening before routine cataract surgery would be beneficial.
    MeSH term(s) Cataract/diagnosis ; Cataract/epidemiology ; Humans ; Prevalence ; Republic of Korea/epidemiology ; Retrospective Studies ; Tomography, Optical Coherence
    Language English
    Publishing date 2021-02-18
    Publishing country Korea (South)
    Document type Journal Article
    ZDB-ID 639346-9
    ISSN 2092-9382 ; 1011-8942
    ISSN (online) 2092-9382
    ISSN 1011-8942
    DOI 10.3341/kjo.2020.0074
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  6. Article ; Online: One-year Outcomes of Ultrathin Descemet Stripping Automated Endothelial Keratoplasty Combined with Cataract Surgery in the Korean Population.

    Koo, Minjeong Ashley / Yoon, Hye Yeon / Park, Jae Hyun / Chung, So-Hyang / Kim, Hyun-Seung / Byun, Yong-Soo

    Korean journal of ophthalmology : KJO

    2024  Volume 38, Issue 2, Page(s) 105–112

    Abstract: Purpose: To evaluate the refractive outcomes after ultrathin Descemet stripping automated endothelial keratoplasty (UT-DSAEK) combined with phacoemulsification and intraocular lens implantation (triple procedure) in the South Korean population.: ... ...

    Abstract Purpose: To evaluate the refractive outcomes after ultrathin Descemet stripping automated endothelial keratoplasty (UT-DSAEK) combined with phacoemulsification and intraocular lens implantation (triple procedure) in the South Korean population.
    Methods: This retrospective observational study included 37 eyes of 36 patients who underwent the UT-DSAEK triple procedure between 2012 and 2021 in a single tertiary hospital. Preoperative and postoperative refractive outcomes and endothelial parameters at 1, 3, 6, and 12 months were observed.
    Results: At the final postoperative 12-month period, the average best-corrected visual acuity was 0.4 ± 0.5 in logarithm of the minimum angle of resolution. The mean endothelial cell density at 12 months was 1,841.92 ± 731.24 cells/mm2, indicating no significant endothelial cell loss compared to the baseline (p = 0.128). The mean postoperative central corneal thickness at 12 months was 597.41 ± 86.26 μm. The postoperative mean absolute error at 12 months was 0.96 ± 0.89 diopters (D) and mean error was 0.89 ± 0.97 D.
    Conclusions: The results of our South Korean cohort study on UT-DSAEK triple surgery showed favorable and safe outcomes. Regardless of graft thickness, it should be noted that a hyperopic shift of 1.00 to 2.00 D needs to be considered in the case of UT-DSAEK triple surgery.
    MeSH term(s) Humans ; Visual Acuity ; Cohort Studies ; Refraction, Ocular ; Corneal Transplantation ; Cataract ; Retrospective Studies ; Descemet Stripping Endothelial Keratoplasty/methods ; Endothelium, Corneal/transplantation ; Fuchs' Endothelial Dystrophy/surgery
    Language English
    Publishing date 2024-02-14
    Publishing country Korea (South)
    Document type Observational Study ; Journal Article
    ZDB-ID 639346-9
    ISSN 2092-9382 ; 1011-8942
    ISSN (online) 2092-9382
    ISSN 1011-8942
    DOI 10.3341/kjo.2023.0101
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  7. Article ; Online: Comparison of pulsed and continuous accelerated corneal crosslinking for keratoconus: 1-year results at a single center.

    Kang, Min-Ji / Hwang, Jehyung / Chung, So-Hyang

    Journal of cataract and refractive surgery

    2020  Volume 47, Issue 5, Page(s) 641–648

    Abstract: Purpose: To compare the clinical outcomes between pulsed and continuous accelerated crosslinking (CXL) for keratoconus.: Setting: Hospital.: Design: Retrospective comparison study.: Methods: Korean patients who were treated for keratoconus ... ...

    Abstract Purpose: To compare the clinical outcomes between pulsed and continuous accelerated crosslinking (CXL) for keratoconus.
    Setting: Hospital.
    Design: Retrospective comparison study.
    Methods: Korean patients who were treated for keratoconus between September 2015 and January 2018 at Seoul St. Mary's Hospital were included. Eyes were subjected to pulsed accelerated crosslinking (30 mW/cm2 for 8 minutes, 1 second on/1 second off) or continuous accelerated crosslinking (30 mW/cm2 for 4 minutes; delivering 7.2 J/cm2). Outcomes were evaluated after 1 year.
    Results: At 1 year, the 2 groups did not exhibit changes in their corrected and uncorrected distance visual acuity values. The pulsed group (25 eyes in 25 patients) exhibited significantly improved values for sphere (P = .009) and spherical equivalent (P = .033), although no statistically significant difference was observed in the continuous group (20 eyes in 20 patients). All keratometry (k)values (SimKf, SimKs, SimKmean, and Kmax) improved in both groups (all P < .05), although the pulsed group had significantly greater changes in the SimKmean value (P = .036) and the Kmax value (P = .03). Both groups had significantly decreased central and thinnest corneal thicknesses (all P < .001), although the pulsed group had a substantially lower thinnest corneal thickness (P = .017). Corneal densitometry measured using the Pentacam device increased in both groups (all P < .001), with a higher densitometry value in the pulsed group (P = .013). Furthermore, the depth of the demarcation line was deeper in the pulsed group (P = .015).
    Conclusions: Pulsed accelerated crosslinking might provide better postcrosslinking effects than continuous accelerated crosslinking.
    MeSH term(s) Collagen/therapeutic use ; Corneal Topography ; Cross-Linking Reagents/therapeutic use ; Humans ; Keratoconus/diagnosis ; Keratoconus/drug therapy ; Photochemotherapy ; Photosensitizing Agents/therapeutic use ; Retrospective Studies ; Riboflavin/therapeutic use ; Ultraviolet Rays
    Chemical Substances Cross-Linking Reagents ; Photosensitizing Agents ; Collagen (9007-34-5) ; Riboflavin (TLM2976OFR)
    Language English
    Publishing date 2020-11-16
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 632744-8
    ISSN 1873-4502 ; 0886-3350
    ISSN (online) 1873-4502
    ISSN 0886-3350
    DOI 10.1097/j.jcrs.0000000000000488
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  8. Article ; Online: Comparison of Corneal Wavefront-optimized and Wavefront-guided Alcohol-assisted Photorefractive Keratectomy Using Schwind Amaris 750S Laser for Myopia.

    Kang, Min Ji / Hwang, Jehyung / Chung, So Hyang

    Korean journal of ophthalmology : KJO

    2020  Volume 34, Issue 3, Page(s) 210–218

    Abstract: Purpose: To compare the visual outcomes and corneal aberrations between wavefront-optimized (WFO) and corneal wavefront-guided (WFG) photorefractive keratectomy (PRK) in low to moderate myopia.: Methods: Twenty-seven eyes treated with WFO and 29 eyes ...

    Abstract Purpose: To compare the visual outcomes and corneal aberrations between wavefront-optimized (WFO) and corneal wavefront-guided (WFG) photorefractive keratectomy (PRK) in low to moderate myopia.
    Methods: Twenty-seven eyes treated with WFO and 29 eyes treated with WFG PRK using a Schwind Amaris 750S Excimer laser were included after 6 months of postoperative follow-up. Uncorrected distance visual acuity, corrected distance visual acuity, refractive errors, corneal higher-order aberrations (HOA) and corneal thickness obtained using a Scheimpflug system, and central ablation depth and volume were evaluated during the preoperative period and again at the postoperative 6-month visits.
    Results: Postoperatively, uncorrected distance visual acuity, corrected distance visual acuity, manifest spherical equivalent, and refractive astigmatism were improved in both groups, and there was no statistically significant difference between the two groups. There was no significant difference in safety, efficacy, or predictability of the refractive outcome. Postoperative total corneal HOA root mean square (RMS), coma RMS, and spherical aberration were significantly increased in both groups. Among these, only spherical aberration showed a significant difference between the two groups, with greater increase in the WFO group at 6 months postoperatively. The changes in corneal HOA RMS and spherical aberration were smaller in the WFG group, and this benefit was marked in eyes with high HOA RMS (≥0.4 μm) and spherical aberration (≥0.2 μm). Even though ablation volume in the WFG group was much larger than that of the WFO group, there was no significant difference in postoperative central and peripheral corneal thickness between the two groups.
    Conclusions: Both WFO and WFG PRK using a Schwind Amaris 750S laser for low to moderate myopia were safe and effective at improving visual and refractive outcomes. However, WFG PRK induced fewer spherical aberrations than WFO PRK and may be more advantageous for eyes with high HOA root mean square or spherical aberration.
    MeSH term(s) Adolescent ; Adult ; Corneal Topography ; Female ; Humans ; Lasers, Excimer/therapeutic use ; Male ; Myopia/pathology ; Myopia/physiopathology ; Myopia/surgery ; Photorefractive Keratectomy/methods ; Postoperative Period ; Refraction, Ocular/physiology ; Retrospective Studies ; Visual Acuity ; Young Adult
    Language English
    Publishing date 2020-05-19
    Publishing country Korea (South)
    Document type Comparative Study ; Journal Article
    ZDB-ID 639346-9
    ISSN 2092-9382 ; 1011-8942
    ISSN (online) 2092-9382
    ISSN 1011-8942
    DOI 10.3341/kjo.2019.0087
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  9. Article ; Online: Comparative Accuracy of Barrett Toric Calculator With and Without Posterior Corneal Astigmatism Measurements and the Kane Toric Formula.

    Yang, Soonwon / Byun, Yong-Soo / Kim, Hyun Seung / Chung, So-Hyang

    American journal of ophthalmology

    2021  Volume 231, Page(s) 48–57

    Abstract: Purpose: To compare the accuracy of the Barrett toric calculator with and without posterior corneal astigmatism and the Kane toric calculator.: Design: Retrospective cross-sectional study.: Methods: The study included a total of 79 eyes of 79 ... ...

    Abstract Purpose: To compare the accuracy of the Barrett toric calculator with and without posterior corneal astigmatism and the Kane toric calculator.
    Design: Retrospective cross-sectional study.
    Methods: The study included a total of 79 eyes of 79 patients who underwent toric intraocular lens (IOL) insertion during uncomplicated cataract surgery by a single surgeon. Using vector analysis, the mean absolute prediction error, the standard deviation of the prediction error, and the percentage of eyes with a prediction error within ±0.50 diopter (D), ± 0.75 D, and ± 1.00 D were calculated. The IOL Master 700 (Carl Zeiss Meditec AG, Jena, Germany) was used for measuring biometry including posterior corneal astigmatism. The main analysis was designed to provide the clinical outcomes with each formula using the postoperative keratometry values and the measured postoperative IOL axis. Real-world analysis was performed using the preoperative keratometry values and the intended IOL axis.
    Results: There was no significant difference in mean absolute prediction errors calculated with 2 versions of the Barrett toric formula (predicted posterior corneal astigmatism and measured posterior corneal astigmatism) and the Kane toric formula (P > .05). The Barrett toric calculator with predicted and measured posterior corneal astigmatism yielded the best results, with 60.8% <0.50 D prediction error in the main analysis. In the real-world analysis, the Barrett toric calculator with predicted posterior corneal astigmatism showed the best result, with 53.2% <0.50 D prediction error.
    Conclusion: The Barrett toric formula with and without posterior corneal astigmatism measurements using the IOL Master 700 and the Kane toric formula yielded accurate and comparable outcomes in this single-surgeon analysis. Am J Ophthalmol 2021;221:•••-•••. © 2021 Elsevier Inc. All rights reserved.
    MeSH term(s) Astigmatism/diagnosis ; Astigmatism/surgery ; Cross-Sectional Studies ; Humans ; Lens Implantation, Intraocular ; Lenses, Intraocular ; Optics and Photonics ; Phacoemulsification ; Refraction, Ocular ; Retrospective Studies
    Language English
    Publishing date 2021-06-09
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80030-2
    ISSN 1879-1891 ; 0002-9394
    ISSN (online) 1879-1891
    ISSN 0002-9394
    DOI 10.1016/j.ajo.2021.05.028
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  10. Article ; Online: Limbal epithelial stem cell sheets from young donors have better regenerative potential.

    Yang, Soonwon / Lee, Hyun Jung / Shin, Soojung / Park, In Yang / Chung, So-Hyang

    Scientific reports

    2022  Volume 12, Issue 1, Page(s) 14191

    Abstract: To investigate the stemness of limbal epithelial stem cell sheets in relation to the donor's age. Human limbal explants from cadaveric donors were set on human amniotic membrane scaffolds with the xeno-free medium. We evaluated limbal epithelial sheet ... ...

    Abstract To investigate the stemness of limbal epithelial stem cell sheets in relation to the donor's age. Human limbal explants from cadaveric donors were set on human amniotic membrane scaffolds with the xeno-free medium. We evaluated limbal epithelial sheet size, expression of stem/progenitor cell markers, and colony formation efficiency from donors of different age groups (age ≤ 45, age 45-65, and age > 65). Expression of the proliferation marker Ki67, stem/progenitor cell markers p63α and ABCG2, cornea specific marker PANCK, and differentiation marker CK12 were evaluated. To determine the effect of donor age on the storage period of limbal explant sheets, the limbal explant outgrowth sheets were stored in 4 °C for 2 days and analyzed for JC-1, p63α, and PANCK with FACS on each day. From days 6 to 12, the outgrowth area of the limbal epithelial stem cell sheet was significantly larger in the age ≤ 45 groups (296 ± 54.7 mm
    MeSH term(s) Aged ; Antigens, Differentiation/metabolism ; Cells, Cultured ; Epithelial Cells/metabolism ; Epithelium, Corneal/metabolism ; Humans ; Limbus Corneae ; Middle Aged ; Stem Cells
    Chemical Substances Antigens, Differentiation
    Language English
    Publishing date 2022-08-19
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-022-17821-9
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