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Article ; Online: Correction: Practical Compass of Single-Cell RNA-Seq Analysis.

Okada, Hiroyuki / Chung, Ung-Il / Hojo, Hironori

2024 Volume 22, Issue 2, Page(s) 299

Language	English
Publishing date	2024-02-19
Publishing country	United States
Document type	Published Erratum
ZDB-ID	2186581-4
ISSN	1544-2241 ; 1544-1873
ISSN (online)	1544-2241
ISSN	1544-1873
DOI	10.1007/s11914-024-00861-7
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Article ; Online: Probing the Molecular Mechanism of Viscoelastic Relaxation in Transient Networks.

Michida, Shota / Chung, Ung-Il / Katashima, Takuya

Gels (Basel, Switzerland)

2023 Volume 9, Issue 12

Abstract: Hydrogels, which have polymer networks through supramolecular and reversible interactions, exhibit various mechanical responsibilities to its surroundings. The influence of the reversible bonds on a hydrogel's macroscopic properties, such as ... ...

Abstract	Hydrogels, which have polymer networks through supramolecular and reversible interactions, exhibit various mechanical responsibilities to its surroundings. The influence of the reversible bonds on a hydrogel's macroscopic properties, such as viscoelasticity and dynamics, is not fully understood, preventing further innovative material development. To understand the relationships between the mechanical properties and molecular structures, it is required to clarify the molecular understanding of the networks solely crosslinked by reversible interactions, termed "transient networks". This review introduces our recent progress on the studies on the molecular mechanism of viscoelasticity in transient networks using multiple methods and model materials. Based on the combination of the viscoelasticity and diffusion measurements, the viscoelastic relaxation of transient networks does not undergo the diffusion of polymers, which is not explained by the framework of conventional molecular models for the viscoelasticity of polymers. Then, we show the results of the comparison between the viscoelastic relaxation and binding dynamics of reversible bonds. Viscoelastic relaxation is primarily affected by "dissociation dynamics of the bonds" and "network structures". These results are explained in the framework that the backbone, which is composed of essential chains supporting the stress, is broken by multiple dissociation events. This understanding of molecular dynamics in viscoelasticity will provide the foundation for designing transient networks.
Language	English
Publishing date	2023-12-01
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2813982-3
ISSN	2310-2861 ; 2310-2861
ISSN (online)	2310-2861
ISSN	2310-2861
DOI	10.3390/gels9120945
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Article ; Online: Practical Compass of Single-Cell RNA-Seq Analysis.

Okada, Hiroyuki / Chung, Ung-Il / Hojo, Hironori

Current osteoporosis reports

2023

Abstract: Purpose of review: This review paper provides step-by-step instructions on the fundamental process, from handling fastq datasets to illustrating plots and drawing trajectories.: Recent findings: The number of studies using single-cell RNA-seq (scRNA- ... ...

Abstract	Purpose of review: This review paper provides step-by-step instructions on the fundamental process, from handling fastq datasets to illustrating plots and drawing trajectories. Recent findings: The number of studies using single-cell RNA-seq (scRNA-seq) is increasing. scRNA-seq revealed the heterogeneity or diversity of the cellular populations. scRNA-seq also provides insight into the interactions between different cell types. User-friendly scRNA-seq packages for ligand-receptor interactions and trajectory analyses are available. In skeletal biology, osteoclast differentiation, fracture healing, ectopic ossification, human bone development, and the bone marrow niche have been examined using scRNA-seq. scRNA-seq data analysis tools are still being developed, even at the fundamental step of dataset integration. However, updating the latest information is difficult for many researchers. Investigators and reviewers must share their knowledge of in silico scRNA-seq for better biological interpretation. This review article aims to provide a useful guide for complex analytical processes in single-cell RNA-seq data analysis.
Language	English
Publishing date	2023-11-29
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2186581-4
ISSN	1544-2241 ; 1544-1873
ISSN (online)	1544-2241
ISSN	1544-1873
DOI	10.1007/s11914-023-00840-4
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Article ; Online: A policy overview of Japan's progress on dementia care in a super-aged society and future challenges.

Ishihara, Miwa / Matsunaga, Sanae / Islam, Rubana / Shibata, Ogusa / Chung, Ung-Il

Global health & medicine

2024 Volume 6, Issue 1, Page(s) 13–18

Abstract: Dementia is highly prevalent in Japan, a super-aged society where almost a third of the population is above 65 years old. Japan has been implementing ageing and dementia policies since 2000 and now has a wealth of experience to share with other nations ... ...

Abstract	Dementia is highly prevalent in Japan, a super-aged society where almost a third of the population is above 65 years old. Japan has been implementing ageing and dementia policies since 2000 and now has a wealth of experience to share with other nations who are anticipating a similar future regarding dementia. This article focuses on the 2019 National Framework for Promotion of Dementia Policies that, based on its philosophy of Inclusion and Risk Reduction, lays out five complementary strategies. Together, these five strategies encourage a whole of society approach in dementia care. We first elaborate on the activities being undertaken under each of these strategies and then discuss the future challenges that Japan needs to address. These policy and social innovations spearheaded by Japan can be useful information for other countries that are anticipating similar future as Japan.
Language	English
Publishing date	2024-02-17
Publishing country	Japan
Document type	Journal Article ; Review
ZDB-ID	3140544-7
ISSN	2434-9194 ; 2434-9194 ; 2434-9186
ISSN (online)	2434-9194
ISSN	2434-9194 ; 2434-9186
DOI	10.35772/ghm.2023.01047
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Article ; Online: Validity and reliability of the Oura Ring Generation 3 (Gen3) with Oura sleep staging algorithm 2.0 (OSSA 2.0) when compared to multi-night ambulatory polysomnography: A validation study of 96 participants and 421,045 epochs.

Svensson, Thomas / Madhawa, Kaushalya / Nt, Hoang / Chung, Ung-Il / Svensson, Akiko Kishi

Sleep medicine

2024 Volume 115, Page(s) 251–263

Abstract: Purpose: To evaluate the validity and the reliability of the Oura Ring Generation 3 (Gen3) with Oura Sleep Staging Algorithm 2.0 (OSSA 2.0) through multi-night polysomnography (PSG).: Participants and methods: Participants were 96 generally healthy ... ...

Abstract	Purpose: To evaluate the validity and the reliability of the Oura Ring Generation 3 (Gen3) with Oura Sleep Staging Algorithm 2.0 (OSSA 2.0) through multi-night polysomnography (PSG). Participants and methods: Participants were 96 generally healthy Japanese men and women aged between 20 and 70 years contributing with 421,045 30-s epochs. Sleep scoring was performed according to American Academy of Sleep Medicine criteria. Each participant could contribute with a maximum of three polysomnography (PSG) nights. Within-participant means were created for each sleep measure and paired t-tests were used to compare equivalent measures obtained from the PSG and Oura Rings (non-dominant and dominant hand). Agreement between sleep measures were assessed using Bland-Altman plots. Interrater reliability for epoch accuracy was determined by prevalence-adjusted and bias-adjusted kappa (PABAK). Results: The Oura Ring did not significantly differ from PSG for the measures time in bed, total sleep time, sleep onset latency, sleep period time, wake after sleep onset, time spent in light sleep, and time spent in deep sleep. Oura Rings worn on the non-dominant- and dominant-hand underestimated sleep efficiency by 1.1 %-1.5 % and time spent in REM sleep by 4.1-5.6 min. The Oura Ring had a sensitivity of 94.4 %-94.5 %, specificity of 73.0 %-74.6 %, a predictive value for sleep of 95.9 %-96.1 %, a predictive value for wake of 66.6 %-67.0 %, and accuracy of 91.7 %-91.8 %. PABAK was 0.83-0.84 and reliability was 94.8 %. Sleep staging accuracy ranged between 75.5 % (light sleep) and 90.6 % (REM sleep). Conclusions: The Oura Ring Gen3 with OSSA 2.0 shows good agreement with PSG for global sleep measures and time spent in light and deep sleep.
MeSH term(s)	Male ; Humans ; Female ; Young Adult ; Adult ; Middle Aged ; Aged ; Polysomnography ; Actigraphy ; Reproducibility of Results ; Sleep ; Algorithms
Language	English
Publishing date	2024-01-26
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2012041-2
ISSN	1878-5506 ; 1389-9457
ISSN (online)	1878-5506
ISSN	1389-9457
DOI	10.1016/j.sleep.2024.01.020
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Article ; Online: Response to comment on "Validity and reliability of the Oura Ring Generation 3 (Gen3) with Oura Sleep Staging Algorithm 2.0 (OSSA 2.0) when compared to multi-night ambulatory polysomnography: A validation study of 96 participants and 421,045 epochs".

Svensson, Thomas / Madhawa, Kaushalya / Nt, Hoang / Chung, Ung-Il / Svensson, Akiko Kishi

Sleep medicine

2024 Volume 117, Page(s) 217–218

MeSH term(s)	Humans ; Polysomnography ; Reproducibility of Results ; Sleep/physiology ; Algorithms
Language	English
Publishing date	2024-03-14
Publishing country	Netherlands
Document type	Letter
ZDB-ID	2012041-2
ISSN	1878-5506 ; 1389-9457
ISSN (online)	1878-5506
ISSN	1389-9457
DOI	10.1016/j.sleep.2024.03.014
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Article ; Online: Association between Metabolic Syndrome Status and Daily Physical Activity Measured by a Wearable Device in Japanese Office Workers.

Yamaga, Yukako / Svensson, Thomas / Chung, Ung-Il / Svensson, Akiko Kishi

International journal of environmental research and public health

2023 Volume 20, Issue 5

Abstract: 1) Background: This study examined the cross-sectional association between metabolic syndrome (MetS) status classified into three groups and daily physical activity (PA; step count and active minutes) using a wearable device in Japanese office workers. ( ...

Abstract	(1) Background: This study examined the cross-sectional association between metabolic syndrome (MetS) status classified into three groups and daily physical activity (PA; step count and active minutes) using a wearable device in Japanese office workers. (2) Methods: This secondary analysis used data from 179 participants in the intervention group of a randomized controlled trial for 3 months. Individuals who had received an annual health check-up and had MetS or were at a high risk of MetS based on Japanese guidelines were asked to use a wearable device and answer questionnaires regarding their daily life for the entire study period. Multilevel mixed-effects logistic regression models adjusted for covariates associated with MetS and PA were used to estimate associations. A sensitivity analysis investigated the associations between MetS status and PA level according to the day of the week. (3) Results: Compared to those with no MetS, those with MetS were not significantly associated with PA, while those with pre-MetS were inversely associated with PA [step count Model 3: OR = 0.60; 95% CI: 0.36, 0.99; active minutes Model 3: OR = 0.62; 95% CI: 0.40, 0.96]. In the sensitivity analysis, day of the week was an effect modifier for both PA (
MeSH term(s)	Humans ; Cross-Sectional Studies ; East Asian People ; Exercise ; Metabolic Syndrome/epidemiology ; Wearable Electronic Devices
Language	English
Publishing date	2023-02-28
Publishing country	Switzerland
Document type	Randomized Controlled Trial ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2175195-X
ISSN	1660-4601 ; 1661-7827
ISSN (online)	1660-4601
ISSN	1661-7827
DOI	10.3390/ijerph20054315
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Article: A Disentangled VAE-BiLSTM Model for Heart Rate Anomaly Detection.

Staffini, Alessio / Svensson, Thomas / Chung, Ung-Il / Svensson, Akiko Kishi

Bioengineering (Basel, Switzerland)

2023 Volume 10, Issue 6

Abstract: Cardiovascular diseases (CVDs) remain a leading cause of death globally. According to the American Heart Association, approximately 19.1 million deaths were attributed to CVDs in 2020, in particular, ischemic heart disease and stroke. Several known risk ... ...

Abstract	Cardiovascular diseases (CVDs) remain a leading cause of death globally. According to the American Heart Association, approximately 19.1 million deaths were attributed to CVDs in 2020, in particular, ischemic heart disease and stroke. Several known risk factors for CVDs include smoking, alcohol consumption, lack of regular physical activity, and diabetes. The last decade has been characterized by widespread diffusion in the use of wristband-style wearable devices which can monitor and collect heart rate data, among other information. Wearable devices allow the analysis and interpretation of physiological and activity data obtained from the wearer and can therefore be used to monitor and prevent potential CVDs. However, these data are often provided in a manner that does not allow the general user to immediately comprehend possible health risks, and often require further analytics to draw meaningful conclusions. In this paper, we propose a disentangled variational autoencoder (
Language	English
Publishing date	2023-06-03
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2746191-9
ISSN	2306-5354
ISSN	2306-5354
DOI	10.3390/bioengineering10060683
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Article ; Online: Tissue-Adhesive Hydrogel Spray System for Live Cell Immobilization on Biological Surfaces.

Ishikawa, Shohei / Kamata, Hiroyuki / Chung, Ung-Il / Sakai, Takamasa

ACS applied bio materials

2023 Volume 6, Issue 11, Page(s) 4613–4619

Abstract: Gelatin hydrogels are used as three-dimensional cell scaffolds and can be prepared using various methods. One widely accepted approach involves crosslinking gelatin amino groups with poly(ethylene glycol) (PEG) modified ... ...

Abstract	Gelatin hydrogels are used as three-dimensional cell scaffolds and can be prepared using various methods. One widely accepted approach involves crosslinking gelatin amino groups with poly(ethylene glycol) (PEG) modified with
MeSH term(s)	Humans ; Hydrogels/chemistry ; Tissue Adhesives ; Gelatin/pharmacology ; Gelatin/chemistry ; Polyethylene Glycols/chemistry ; Biocompatible Materials/chemistry
Chemical Substances	Hydrogels ; Tissue Adhesives ; Gelatin (9000-70-8) ; Polyethylene Glycols (3WJQ0SDW1A) ; Biocompatible Materials
Language	English
Publishing date	2023-07-19
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2576-6422
ISSN (online)	2576-6422
DOI	10.1021/acsabm.3c00378
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Article ; Online: Sleep Satisfaction May Modify the Association between Metabolic Syndrome and BMI, Respectively, and Occupational Stress in Japanese Office Workers.

Pham, Helena / Svensson, Thomas / Chung, Ung-Il / Svensson, Akiko Kishi

International journal of environmental research and public health

2022 Volume 19, Issue 9

Abstract: The association between obesity and psychological stress is ambiguous. The aim is to investigate the association between metabolic syndrome (MetS) and body mass index (BMI), respectively, with occupational stress among Japanese office workers. The study ... ...

Abstract	The association between obesity and psychological stress is ambiguous. The aim is to investigate the association between metabolic syndrome (MetS) and body mass index (BMI), respectively, with occupational stress among Japanese office workers. The study is a secondary analysis of the intervention group from a randomized controlled trial. There are 167 participants included in the analysis. Occupational stress is self-reported using the Brief Job Stress Questionnaire (BJSQ). BMI and the classification of MetS/pre-MetS was based on the participants' annual health check-up data. The primary exposure is divided into three groups: no MetS, pre-MetS, and MetS in accordance with Japanese guidelines. The secondary exposure, BMI, remains as a continuous variable. Multiple linear regression is implemented. Sensitivity analyses are stratified by sleep satisfaction. Pre-MetS is significantly associated with occupational stress (7.84 points; 95% CI: 0.17, 15.51). Among participants with low sleep satisfaction, pre-MetS (14.09 points; 95% CI: 1.71, 26.48), MetS (14.72 points; 95% CI: 0.93, 28.51), and BMI (2.54 points; 95% CI: 0.05, 4.99) are all significantly associated with occupational stress. No significant associations are observed in participants with high sleep satisfaction. The findings of this study indicate that sleep satisfaction may modify the association between MetS and BMI, respectively, and occupational stress.
MeSH term(s)	Body Mass Index ; Humans ; Japan/epidemiology ; Metabolic Syndrome/epidemiology ; Occupational Stress/epidemiology ; Personal Satisfaction ; Risk Factors ; Sleep
Language	English
Publishing date	2022-04-22
Publishing country	Switzerland
Document type	Journal Article ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't
ZDB-ID	2175195-X
ISSN	1660-4601 ; 1661-7827
ISSN (online)	1660-4601
ISSN	1661-7827
DOI	10.3390/ijerph19095095
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