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  1. Article ; Online: Potential Significance of Serum Autoantibodies to Endometrial Antigens, α-Enolase and Hormones in Non-Invasive Diagnosis and Pathogenesis of Endometriosis.

    Menzhinskaya, Irina V / Pavlovich, Stanislav V / Melkumyan, Arika G / Chuprynin, Vladimir D / Yarotskaya, Ekaterina L / Sukhikh, Gennady T

    International journal of molecular sciences

    2023  Volume 24, Issue 21

    Abstract: The objective of the study was to evaluate the profile of serum autoantibodies and their diagnostic and pathogenetic significance in ovarian endometrioma (OEM) and deep infiltrative endometriosis (DIE). The study enrolled 74 patients with endometriosis ( ... ...

    Abstract The objective of the study was to evaluate the profile of serum autoantibodies and their diagnostic and pathogenetic significance in ovarian endometrioma (OEM) and deep infiltrative endometriosis (DIE). The study enrolled 74 patients with endometriosis (Group 1), including 53 patients with OEM (Subgroup 1a); 21 patients with DIE without ovarian lesions (Subgroup 1b); and 27 patients without endometriosis (Group 2). The diagnosis was confirmed by laparoscopic surgery and histologic examination of resected tissues. Antibodies (M, G) to tropomyosin 3 (TPM), tropomodulin 3 (TMOD), α-enolase (ENO), estradiol (E2), progesterone (PG), and human chorionic gonadotropin (hCG) were identified in blood serum using modified ELISA. In endometriosis, antibodies to endometrial antigens, hormones, and ENO were detected more often than antiphospholipid and antinuclear antibodies. Higher levels of IgM to TPM, hCG, E2, and PG and IgG to TMOD, ENO, E2, and hCG were found in Subgroup 1a compared to Group 2. IgM to TPM, hCG, E2, PG, and IgG to E2 and ENO had a high diagnostic value for OEM (AUC > 0.7), with antibodies to TPM having the highest sensitivity and specificity (73.6% and 81.5%). In Subgroup 1b, only the levels of IgM to TPM and hCG were higher than in Group 2. These antibodies had a high diagnostic value for DIE. Thus, endometriosis is associated with autoantibodies to endometrial antigens, α-enolase, steroid, and gonadotropic hormones. A wider spectrum of antibodies is detected in OEM than in DIE. These antibodies have a high diagnostic value for OEM and DIE and potential pathogenetic significance for endometriosis and associated infertility.
    MeSH term(s) Female ; Humans ; Endometriosis ; Ovarian Cysts ; Ovarian Neoplasms ; Autoantibodies ; Progesterone ; Chorionic Gonadotropin ; Immunoglobulin G ; Phosphopyruvate Hydratase ; Immunoglobulin M
    Chemical Substances Autoantibodies ; Progesterone (4G7DS2Q64Y) ; Chorionic Gonadotropin ; Immunoglobulin G ; Phosphopyruvate Hydratase (EC 4.2.1.11) ; Immunoglobulin M
    Language English
    Publishing date 2023-10-25
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms242115578
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Altered Glycolysis, Mitochondrial Biogenesis, Autophagy and Apoptosis in Peritoneal Endometriosis in Adolescents.

    Khashchenko, Elena P / Vysokikh, Mikhail Yu / Marey, Maria V / Sidorova, Ksenia O / Manukhova, Ludmila A / Shkavro, Natalya N / Uvarova, Elena V / Chuprynin, Vladimir D / Fatkhudinov, Timur Kh / Adamyan, Leila V / Sukhikh, Gennady T

    International journal of molecular sciences

    2024  Volume 25, Issue 8

    Abstract: Energy metabolism plays a pivotal role in the pathogenesis of endometriosis. For the initial stages of the disease in adolescents, this aspect remains unexplored. The objective of this paper was to analyze the association of cellular and endosomal ... ...

    Abstract Energy metabolism plays a pivotal role in the pathogenesis of endometriosis. For the initial stages of the disease in adolescents, this aspect remains unexplored. The objective of this paper was to analyze the association of cellular and endosomal profiles of markers of glycolysis, mitochondrial biogenesis, apoptosis, autophagy and estrogen signaling in peritoneal endometriosis (PE) in adolescents. We included 60 girls aged 13-17 years in a case-control study: 45 with laparoscopically confirmed PE (main group) and 15 with paramesonephric cysts (comparison group). Samples of plasma and peritoneal fluid exosomes, endometrioid foci and non-affected peritoneum were tested for estrogen receptor (Erα/β), hexokinase (Hex2), pyruvate dehydrogenase kinase (PDK1), glucose transporter (Glut1), monocarboxylate transporters (MCT1 and MCT2), optic atrophy 1 (OPA1, mitochondrial fusion protein), dynamin-related protein 1 (DRP1, mitochondrial fission protein), Bax, Bcl2, Beclin1, Bnip3, P38 mitogen-activated protein kinase (MAPK), hypoxia-inducible factor 1 (Hif-1α), mitochondrial voltage-dependent anion channel (VDAC) and transforming growth factor (TGFβ) proteins as markers of estrogen signaling, glycolysis rates, mitochondrial biogenesis and damage, apoptosis and autophagy (Western-Blot and PCR). The analysis identified higher levels of molecules associated with proliferation (ERβ), glycolysis (MCT2, PDK1, Glut1, Hex2, TGFβ and Hif-1α), mitochondrial biogenesis (OPA1, DRP1) and autophagy (P38, Beclin1 and Bnip3) and decreased levels of apoptosis markers (
    MeSH term(s) Female ; Humans ; Adolescent ; Endometriosis/metabolism ; Endometriosis/pathology ; Autophagy ; Apoptosis ; Glycolysis ; Case-Control Studies ; Organelle Biogenesis ; Estrogen Receptor beta/metabolism ; Signal Transduction ; Estrogen Receptor alpha/metabolism ; Biomarkers
    Chemical Substances Estrogen Receptor beta ; Estrogen Receptor alpha ; Biomarkers
    Language English
    Publishing date 2024-04-11
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms25084238
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Endometriosis in Adolescents: Diagnostics, Clinical and Laparoscopic Features.

    Khashchenko, Elena P / Uvarova, Elena V / Fatkhudinov, Timur Kh / Chuprynin, Vladimir D / Asaturova, Aleksandra V / Kulabukhova, Elena A / Vysokikh, Mikhail Yu / Allakhverdieva, Elvina Z / Alekseeva, Maria N / Adamyan, Leila V / Sukhikh, Gennady T

    Journal of clinical medicine

    2023  Volume 12, Issue 4

    Abstract: Background: The early diagnosis of endometriosis in adolescents is not developed.: Objective: We aim to conduct clinical, imaging, laparoscopic and histological analyses of peritoneal endometriosis (PE) in adolescents in order to improve early ... ...

    Abstract Background: The early diagnosis of endometriosis in adolescents is not developed.
    Objective: We aim to conduct clinical, imaging, laparoscopic and histological analyses of peritoneal endometriosis (PE) in adolescents in order to improve early diagnosis.
    Methods: In total, 134 girls (from menarche to 17 years old) were included in a case-control study: 90 with laparoscopically (LS) confirmed PE, 44 healthy controls underwent full examination and LS was analyzed in the PE group.
    Results: Patients with PE were characterized with heredity for endometriosis, persistent dysmenorrhea, decreased daily activity, gastrointestinal symptoms, higher LH, estradiol, prolactin and Ca-125 (<0.05 for each). Ultrasound detected PE in 3.3% and MRI in 78.9%. The most essential MRI signs are as follows: hypointense foci, the heterogeneity of the pelvic tissue (paraovarian, parametrial and rectouterine pouch) and sacro-uterine ligaments lesions (<0.05 for each). Adolescents with PE mostly exhibit initial rASRM stages. Red implants correlated with the rASRM score, and sheer implants correlated with pain (VAS score) (<0.05). In 32.2%, foci consisted of fibrous, adipose and muscle tissue; black lesions were more likely to be histologically verified (0.001).
    Conclusion: Adolescents exhibit mostly initial PE stages, which are associated with greater pain. Persistent dysmenorrhea and detected MRI parameters predict the laparoscopic confirmation of initial PE in adolescents in 84.3% (OR 15.4; <0.01), justifying the early surgical diagnostics and shortening the time delay and suffering of the young patients.
    Language English
    Publishing date 2023-02-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662592-1
    ISSN 2077-0383
    ISSN 2077-0383
    DOI 10.3390/jcm12041678
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Pelvic Pain, Mental Health and Quality of Life in Adolescents with Endometriosis after Surgery and Dienogest Treatment.

    Khashchenko, Elena P / Uvarova, Elena V / Chuprynin, Vladimir D / Pustynnikova, Margarita Yu / Fatkhudinov, Timur Kh / Elchaninov, Andrey V / Gardanova, Zhanna R / Ivanets, Tatyana Yu / Vysokikh, Mikhail Yu / Sukhikh, Gennady T

    Journal of clinical medicine

    2023  Volume 12, Issue 6

    Abstract: Background: Diagnostic and treatment delays have caused significant impacts on the physical and emotional well-being of adolescents with endometriosis, though such research is limited. This study aimed to assess the effects of one-year dienogest therapy ...

    Abstract Background: Diagnostic and treatment delays have caused significant impacts on the physical and emotional well-being of adolescents with endometriosis, though such research is limited. This study aimed to assess the effects of one-year dienogest therapy on the clinical picture, pain patterns, psycho-emotional status, and quality-of-life indicators in adolescents with endometriosis after surgical treatment.
    Methods: The study enrolled 32 girls aged 13-17 with peritoneal endometriosis to analyze one-year dynamics of the Visual Analog Scale (VAS), McGill Pain Questionnaire, Beck Depression Scale (BDI), Hospital Anxiety and Depression Scale (HADS), Spielberger State-Trait Anxiety Inventory (STAI) and SF-36 quality-of-life survey scores along with clinical and laboratory indicators before surgery and after one-year dienogest therapy.
    Results: The therapy provided a significant alleviation of endometriosis-associated clinical symptoms, including dysmenorrhea, pelvic pain, gastrointestinal/dysuria symptoms, decreased everyday activity (<0.001), a decrease in anxiety/depression scores (BDI, HADS, STAI), and quality-of-life improvement (<0.001). These effects were accompanied by beneficial dynamics in hormone and inflammatory markers (prolactin, cortisol, testosterone, estradiol, CA-125, neutrophil/lymphocyte ratio; <0.005) within reference ranges. A low body mass index and high C-reactive protein levels were associated with higher VAS scores; a high estradiol level was a factor for anxiety/depression aggravation (<0.05).
    Conclusions: Dienogest, after surgical treatment, significantly improved quality of life and reduced pain symptoms while showing good tolerability and compliance, and reasoning with timely hormonal therapy in adolescents with endometriosis.
    Language English
    Publishing date 2023-03-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662592-1
    ISSN 2077-0383
    ISSN 2077-0383
    DOI 10.3390/jcm12062400
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: The Levels of Ghrelin, Glucagon, Visfatin and Glp-1 Are Decreased in the Peritoneal Fluid of Women with Endometriosis along with the Increased Expression of the CD10 Protease by the Macrophages.

    Krasnyi, Aleksey M / Sadekova, Alsu A / Smolnova, Tatyana Y / Chursin, Vyacheslav V / Buralkina, Natalya A / Chuprynin, Vladimir D / Yarotskaya, Ekaterina / Pavlovich, Stanislav V / Sukhikh, Gennadiy T

    International journal of molecular sciences

    2022  Volume 23, Issue 18

    Abstract: The aim of this study was to evaluate the levels of ten energy metabolism factors: C-peptide, ghrelin, GIP, GLP-1, glucagon, insulin, leptin, PAI-1 (total), resistin, and visfatin, and to determine the expression of GLP1R receptors, CD10, CD26 proteases, ...

    Abstract The aim of this study was to evaluate the levels of ten energy metabolism factors: C-peptide, ghrelin, GIP, GLP-1, glucagon, insulin, leptin, PAI-1 (total), resistin, and visfatin, and to determine the expression of GLP1R receptors, CD10, CD26 proteases, and pro-inflammatory marker CD86 by macrophages in the peritoneal fluid (PF) in patients with endometriosis. The study included 54 women with endometriosis and a control group of 30 women with uterine myoma without signs of endometriosis. The levels of factors in PF were assessed by a multiplex method. Expression of GLP1R receptors, CD10, CD26 proteases, and CD86 by macrophages was evaluated using flow cytometry. It was found that in women with endometriosis, the concentrations of ghrelin, GLP-1, glucagon, and visfatin in PF were reduced (
    MeSH term(s) Ascitic Fluid/metabolism ; Biomarkers/metabolism ; C-Peptide/metabolism ; Dipeptidyl Peptidase 4/metabolism ; Endometriosis/metabolism ; Female ; Ghrelin/metabolism ; Glucagon/metabolism ; Glucagon-Like Peptide 1/metabolism ; Humans ; Leptin/metabolism ; Macrophages/metabolism ; Nicotinamide Phosphoribosyltransferase/metabolism ; Peptide Hydrolases/metabolism ; Plasminogen Activator Inhibitor 1/metabolism ; Resistin/metabolism
    Chemical Substances Biomarkers ; C-Peptide ; Ghrelin ; Leptin ; Plasminogen Activator Inhibitor 1 ; Resistin ; Glucagon-Like Peptide 1 (89750-14-1) ; Glucagon (9007-92-5) ; Nicotinamide Phosphoribosyltransferase (EC 2.4.2.12) ; Peptide Hydrolases (EC 3.4.-) ; Dipeptidyl Peptidase 4 (EC 3.4.14.5)
    Language English
    Publishing date 2022-09-08
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms231810361
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Mesenchymal Stromal Cells Isolated from Ectopic but Not Eutopic Endometrium Display Pronounced Immunomodulatory Activity In Vitro.

    Lupatov, Alexey Yu / Saryglar, Roza Yu / Vtorushina, Valentina V / Poltavtseva, Rimma A / Bystrykh, Oxana A / Chuprynin, Vladimir D / Krechetova, Lyubov V / Pavlovich, Stanislav V / Yarygin, Konstantin N / Sukhikh, Gennady T

    Biomedicines

    2021  Volume 9, Issue 10

    Abstract: A comparative analysis of the cell surface markers and immunological properties of cell cultures originating from normal endometrium and endometrioid heterotopias of women with extragenital endometriosis was carried out. Both types of cell cultures ... ...

    Abstract A comparative analysis of the cell surface markers and immunological properties of cell cultures originating from normal endometrium and endometrioid heterotopias of women with extragenital endometriosis was carried out. Both types of cell cultures expressed surface molecules typical of mesenchymal stromal cells and did not express hematopoietic and epithelial markers. Despite similar phenotype, the mesenchymal stromal cells derived from the two sources had different immunomodulation capacities: the cells of endometrioid heterotopias but not eutopic endometrium could suppress dendritic cell differentiation from monocytes as well as lymphocyte proliferation in allogeneic co-cultures. A comparative multiplex analysis of the secretomes revealed a significant increase in the secretion of pro-inflammatory mediators, including IL6, IFN-γ, and several chemokines associated with inflammation by the stromal cells of ectopic lesions. The results demonstrate that the stromal cells of endometrioid heterotopias display enhanced pro-inflammatory and immunosuppressive activities, which most likely impact the pathogenesis and progression of the disease.
    Language English
    Publishing date 2021-09-22
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720867-9
    ISSN 2227-9059
    ISSN 2227-9059
    DOI 10.3390/biomedicines9101286
    Database MEDical Literature Analysis and Retrieval System OnLINE

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