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  1. Article ; Online: Human gasdermin D and MLKL disrupt mitochondria, endocytic traffic and TORC1 signalling in budding yeast.

    Valenti, Marta / Molina, María / Cid, Víctor J

    Open biology

    2023  Volume 13, Issue 5, Page(s) 220366

    Abstract: Gasdermin D (GSDMD) and mixed lineage kinase domain-like protein (MLKL) are the pore-forming effectors of pyroptosis and necroptosis, respectively, with the capacity to disturb plasma membrane selective permeability and induce regulated cell death. The ... ...

    Abstract Gasdermin D (GSDMD) and mixed lineage kinase domain-like protein (MLKL) are the pore-forming effectors of pyroptosis and necroptosis, respectively, with the capacity to disturb plasma membrane selective permeability and induce regulated cell death. The budding yeast
    MeSH term(s) Humans ; Gasdermins ; Mitochondria ; Protein Kinases ; Saccharomyces cerevisiae ; Transcription Factors ; Endocytosis ; Mechanistic Target of Rapamycin Complex 1
    Chemical Substances Gasdermins ; MLKL protein, human (EC 2.7.-) ; Protein Kinases (EC 2.7.-) ; Transcription Factors ; GSDMD protein, human ; Mechanistic Target of Rapamycin Complex 1 (EC 2.7.11.1)
    Language English
    Publishing date 2023-05-24
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2630944-0
    ISSN 2046-2441 ; 2046-2441
    ISSN (online) 2046-2441
    ISSN 2046-2441
    DOI 10.1098/rsob.220366
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Heterologous Expression and Auto-Activation of Human Pro-Inflammatory Caspase-1 in

    Valenti, Marta / Molina, María / Cid, Víctor J

    Frontiers in immunology

    2021  Volume 12, Page(s) 668602

    Abstract: Caspases are a family of cysteine proteases that play an essential role in inflammation, apoptosis, cell death, and development. Here we delve into the effects caused by heterologous expression of human caspase-1 in the ... ...

    Abstract Caspases are a family of cysteine proteases that play an essential role in inflammation, apoptosis, cell death, and development. Here we delve into the effects caused by heterologous expression of human caspase-1 in the yeast
    MeSH term(s) Actin Cytoskeleton/enzymology ; Actin Cytoskeleton/genetics ; Caspase 1/biosynthesis ; Caspase 1/genetics ; Caspase 8/biosynthesis ; Caspase 8/genetics ; Enzyme Activation ; Enzyme Induction ; Galactokinase/genetics ; Galactokinase/metabolism ; Gene Expression Regulation, Fungal ; Humans ; Intracellular Signaling Peptides and Proteins/genetics ; Intracellular Signaling Peptides and Proteins/metabolism ; Microbial Viability ; Microfilament Proteins/genetics ; Microfilament Proteins/metabolism ; Mitochondria/enzymology ; Mitochondria/genetics ; Phosphate-Binding Proteins/genetics ; Phosphate-Binding Proteins/metabolism ; Saccharomyces cerevisiae/enzymology ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/growth & development ; Saccharomyces cerevisiae Proteins/genetics ; Saccharomyces cerevisiae Proteins/metabolism ; Substrate Specificity
    Chemical Substances Bni1 protein, S cerevisiae ; GSDMD protein, human ; Intracellular Signaling Peptides and Proteins ; Microfilament Proteins ; Phosphate-Binding Proteins ; Saccharomyces cerevisiae Proteins ; GAL1 protein, S cerevisiae (EC 2.7.1.6) ; Galactokinase (EC 2.7.1.6) ; CASP8 protein, human (EC 3.4.22.-) ; Caspase 8 (EC 3.4.22.-) ; Caspase 1 (EC 3.4.22.36)
    Language English
    Publishing date 2021-07-14
    Publishing country Switzerland
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2021.668602
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Teaching microbiology in times of plague.

    Sánchez-Angulo, Manuel / López-Goñi, Ignacio / Cid, Víctor J

    International microbiology : the official journal of the Spanish Society for Microbiology

    2021  Volume 24, Issue 4, Page(s) 665–670

    Abstract: The COVID-19 pandemic has imposed several challenges and strains at all levels of the educational system, especially as a consequence of lockdown and social distance measures. After a period of exclusive use of the online educational environment, ... ...

    Abstract The COVID-19 pandemic has imposed several challenges and strains at all levels of the educational system, especially as a consequence of lockdown and social distance measures. After a period of exclusive use of the online educational environment, educators have adapted to the new circumstances and, by a combination of different strategies, have fought to overcome the limitations and deficiencies of virtual learning. Student motivation, productivity, and creativity continue to be the main pedagogical issues that have to be reached with the new didactic tools developed during the pandemic. At the same time, this pandemic has shown the importance of the inclusion of microbiology as a core element of the educational curriculum and the opportunity to raise public awareness of the importance of microbes to everyday life.
    MeSH term(s) COVID-19/epidemiology ; COVID-19/psychology ; Curriculum ; Education, Distance/methods ; Humans ; Learning ; Microbiology/education ; Teaching/psychology
    Language English
    Publishing date 2021-05-03
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 1454951-7
    ISSN 1618-1905 ; 1139-6709
    ISSN (online) 1618-1905
    ISSN 1139-6709
    DOI 10.1007/s10123-021-00179-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4964: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  4. Article ; Online: Heterologous Expression and Assembly of Human TLR Signaling Components in

    Coronas-Serna, Julia María / Del Val, Elba / Kagan, Jonathan C / Molina, María / Cid, Víctor J

    Biomolecules

    2021  Volume 11, Issue 11

    Abstract: Toll-like receptor (TLR) signaling is key to detect pathogens and initiating inflammation. Ligand recognition triggers the assembly of supramolecular organizing centers (SMOCs) consisting of large complexes composed of multiple subunits. Building such ... ...

    Abstract Toll-like receptor (TLR) signaling is key to detect pathogens and initiating inflammation. Ligand recognition triggers the assembly of supramolecular organizing centers (SMOCs) consisting of large complexes composed of multiple subunits. Building such signaling hubs relies on Toll Interleukin-1 Receptor (TIR) and Death Domain (DD) protein-protein interaction domains. We have expressed TIR domain-containing components of the human myddosome (TIRAP and MyD88) and triffosome (TRAM and TRIF) SMOCs in
    MeSH term(s) Adaptor Proteins, Signal Transducing ; Saccharomyces cerevisiae ; Signal Transduction ; Toll-Like Receptor 4 ; Toll-Like Receptors
    Chemical Substances Adaptor Proteins, Signal Transducing ; Toll-Like Receptor 4 ; Toll-Like Receptors
    Language English
    Publishing date 2021-11-22
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2701262-1
    ISSN 2218-273X ; 2218-273X
    ISSN (online) 2218-273X
    ISSN 2218-273X
    DOI 10.3390/biom11111737
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Survival of Salmonella inside activated macrophages - why bacteria will not understand the word NO?

    Cid, Victor J

    Microbiology (Reading, England)

    2009  Volume 155, Issue Pt 8, Page(s) 2461

    MeSH term(s) Animals ; Host-Pathogen Interactions ; Humans ; Macrophages/metabolism ; Macrophages/microbiology ; Nitric Oxide/metabolism ; Salmonella/pathogenicity ; Salmonella/physiology ; Salmonella Infections/metabolism ; Salmonella Infections/microbiology ; Virulence
    Chemical Substances Nitric Oxide (31C4KY9ESH)
    Language English
    Publishing date 2009-07-09
    Publishing country England
    Document type Comment ; Journal Article
    ZDB-ID 1180712-x
    ISSN 1465-2080 ; 1350-0872
    ISSN (online) 1465-2080
    ISSN 1350-0872
    DOI 10.1099/mic.0.032219-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.140: Show issues Location:
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  6. Article ; Online: A trans-kingdom T6SS effector induces the fragmentation of the mitochondrial network and activates innate immune receptor NLRX1 to promote infection.

    Sá-Pessoa, Joana / López-Montesino, Sara / Przybyszewska, Kornelia / Rodríguez-Escudero, Isabel / Marshall, Helina / Ova, Adelia / Schroeder, Gunnar N / Barabas, Peter / Molina, María / Curtis, Tim / Cid, Víctor J / Bengoechea, José A

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 871

    Abstract: Bacteria can inhibit the growth of other bacteria by injecting effectors using a type VI secretion system (T6SS). T6SS effectors can also be injected into eukaryotic cells to facilitate bacterial survival, often by targeting the cytoskeleton. Here, we ... ...

    Abstract Bacteria can inhibit the growth of other bacteria by injecting effectors using a type VI secretion system (T6SS). T6SS effectors can also be injected into eukaryotic cells to facilitate bacterial survival, often by targeting the cytoskeleton. Here, we show that the trans-kingdom antimicrobial T6SS effector VgrG4 from Klebsiella pneumoniae triggers the fragmentation of the mitochondrial network. VgrG4 colocalizes with the endoplasmic reticulum (ER) protein mitofusin 2. VgrG4 induces the transfer of Ca
    MeSH term(s) Cullin Proteins/metabolism ; NF-KappaB Inhibitor alpha ; NF-kappa B/metabolism ; Reactive Oxygen Species/metabolism ; Immunity, Innate
    Chemical Substances Cullin Proteins ; NF-KappaB Inhibitor alpha (139874-52-5) ; NF-kappa B ; Reactive Oxygen Species
    Language English
    Publishing date 2023-02-16
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-36629-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Microbiology in the "-omics" era: a report on the 2nd FEMS Congress (Madrid, 4-8 July 2006).

    Cid, Víctor J

    International microbiology : the official journal of the Spanish Society for Microbiology

    2005  Volume 9, Issue 4, Page(s) 303–308

    MeSH term(s) Animals ; Biodegradation, Environmental ; Birds ; Genomics/methods ; Influenza in Birds/epidemiology ; Microbiology
    Language English
    Publishing date 2005-11-08
    Publishing country Switzerland
    Document type Congress
    ZDB-ID 1454951-7
    ISSN 1618-1905 ; 1139-6709
    ISSN (online) 1618-1905
    ISSN 1139-6709
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4964: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  8. Article ; Online: Functional analysis of PTEN variants of unknown significance from PHTS patients unveils complex patterns of PTEN biological activity in disease.

    Torices, Leire / Mingo, Janire / Rodríguez-Escudero, Isabel / Fernández-Acero, Teresa / Luna, Sandra / Nunes-Xavier, Caroline E / López, José I / Mercadillo, Fátima / Currás, María / Urioste, Miguel / Molina, María / Cid, Víctor J / Pulido, Rafael

    European journal of human genetics : EJHG

    2022  Volume 31, Issue 5, Page(s) 568–577

    Abstract: Heterozygous germline mutations in PTEN gene predispose to hamartomas and tumors in different tissues, as well as to neurodevelopmental disorders, and define at genetic level the PTEN Hamartoma Tumor Syndrome (PHTS). The major physiologic role of PTEN ... ...

    Abstract Heterozygous germline mutations in PTEN gene predispose to hamartomas and tumors in different tissues, as well as to neurodevelopmental disorders, and define at genetic level the PTEN Hamartoma Tumor Syndrome (PHTS). The major physiologic role of PTEN protein is the dephosphorylation of phosphatidylinositol (3,4,5)-trisphosphate (PIP3), counteracting the pro-oncogenic function of phosphatidylinositol 3-kinase (PI3K), and PTEN mutations in PHTS patients frequently abrogate PTEN PIP3 catalytic activity. PTEN also displays non-canonical PIP3-independent functions, but their involvement in PHTS pathogeny is less understood. We have previously identified and described, at clinical and genetic level, novel PTEN variants of unknown functional significance in PHTS patients. Here, we have performed an extensive functional characterization of these PTEN variants (c.77 C > T, p.(Thr26Ile), T26I; c.284 C > G, p.(Pro95Arg), P95R; c.529 T > A, p.(Tyr177Asn), Y177N; c.781 C > G, p.(Gln261Glu), Q261E; c.829 A > G, p.(Thr277Ala), T277A; and c.929 A > G, p.(Asp310Gly), D310G), including cell expression levels and protein stability, PIP3-phosphatase activity, and subcellular localization. In addition, caspase-3 cleavage analysis in cells has been assessed using a C2-domain caspase-3 cleavage-specific anti-PTEN antibody. We have found complex patterns of functional activity on PTEN variants, ranging from loss of PIP3-phosphatase activity, diminished protein expression and stability, and altered nuclear/cytoplasmic localization, to intact functional properties, when compared with PTEN wild type. Furthermore, we have found that PTEN cleavage at the C2-domain by the pro-apoptotic protease caspase-3 is diminished in specific PTEN PHTS variants. Our findings illustrate the multifaceted molecular features of pathogenic PTEN protein variants, which could account for the complexity in the genotype/phenotype manifestations of PHTS patients.
    MeSH term(s) Humans ; Caspase 3/genetics ; Germ-Line Mutation ; Hamartoma Syndrome, Multiple/genetics ; Phosphatidylinositol 3-Kinases/genetics ; PTEN Phosphohydrolase/genetics ; PTEN Phosphohydrolase/metabolism
    Chemical Substances Caspase 3 (EC 3.4.22.-) ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; PTEN Phosphohydrolase (EC 3.1.3.67) ; PTEN protein, human (EC 3.1.3.67)
    Language English
    Publishing date 2022-12-21
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1141470-4
    ISSN 1476-5438 ; 1018-4813
    ISSN (online) 1476-5438
    ISSN 1018-4813
    DOI 10.1038/s41431-022-01265-w
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3589: Show issues Location:
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  9. Article ; Online: Optimizing Small World Initiative service learning by focusing on antibiotics-producing actinomycetes from soil.

    de Groot, Piet W J / Fernández-Pereira, Jordan / Sabariegos, Rosario / Clemente-Casares, Pilar / Parra-Martínez, Javier / Cid, Víctor J / Moreno, Diego A

    FEMS microbiology letters

    2020  Volume 366, Issue 24

    Abstract: Small World Initiative and Tiny Earth are popular citizen science programs that are implemented worldwide in response to the global antibiotic resistance crisis. When starting up the program in Albacete (Spain), we noted that rates of isolated antibiotic- ...

    Abstract Small World Initiative and Tiny Earth are popular citizen science programs that are implemented worldwide in response to the global antibiotic resistance crisis. When starting up the program in Albacete (Spain), we noted that rates of isolated antibiotic-producing bacteria are generally low. To make the activity more stimulating for participating students, we modified the protocol to obtain more positive results by focusing on isolation of actinomycetes, the main producers of most clinically used antibiotics. Adaptations involved redesigning culture media, incubation times and temperatures, and modification of the ESKAPE antibiosis experiment by employing an agar-transplantation step. Of 390 bacterial isolates tested, almost 6% tested positive in antibiosis experiments and DNA sequence analysis confirmed that all positives are actinomycetes, demonstrating that our protocol is efficient toward isolating antibiotic-producing actinomycetes from soil. Evaluation forms filled by participating students indicated that the program was received very positively and that our modifications contribute to make this educational program more stimulating and efficient.
    MeSH term(s) Actinobacteria/isolation & purification ; Actinobacteria/metabolism ; Anti-Bacterial Agents/metabolism ; Drug Resistance, Microbial ; Microbial Sensitivity Tests ; Soil Microbiology
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2020-01-29
    Publishing country England
    Document type Journal Article
    ZDB-ID 752343-9
    ISSN 1574-6968 ; 0378-1097
    ISSN (online) 1574-6968
    ISSN 0378-1097
    DOI 10.1093/femsle/fnaa019
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 1372: Show issues Location:
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  10. Article: A humanized yeast-based toolkit for monitoring phosphatidylinositol 3-kinase activity at both single cell and population levels.

    Coronas-Serna, Julia María / Fernández-Acero, Teresa / Molina, María / Cid, Víctor J

    Microbial cell (Graz, Austria)

    2018  Volume 5, Issue 12, Page(s) 545–554

    Abstract: Phosphatidylinositol 3-kinase (PI3K) is a key regulator of phosphoinositide-dependent signaling in mammalian cells and its dysfunction is related to multiple syndromes, including cancer. By heterologous expression ... ...

    Abstract Phosphatidylinositol 3-kinase (PI3K) is a key regulator of phosphoinositide-dependent signaling in mammalian cells and its dysfunction is related to multiple syndromes, including cancer. By heterologous expression in
    Language English
    Publishing date 2018-11-12
    Publishing country Austria
    Document type Journal Article
    ZDB-ID 2814756-X
    ISSN 2311-2638
    ISSN 2311-2638
    DOI 10.15698/mic2018.12.660
    Database MEDical Literature Analysis and Retrieval System OnLINE

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