LIVIVO - Search results -

Search results

Result 1 - 10 of total 66

Search options

Article ; Online: Strengthening ancient mtDNA equid sequences from Pompeii.

Cipollaro, Marilena

2011 Volume 112, Issue 2, Page(s) 363–364

MeSH term(s)	Animals ; Base Sequence ; DNA, Mitochondrial/genetics ; Equidae/genetics ; Italy
Chemical Substances	DNA, Mitochondrial
Language	English
Publishing date	2011-02
Publishing country	United States
Document type	Comment ; Journal Article
ZDB-ID	392402-6
ISSN	1097-4644 ; 0730-2312
ISSN (online)	1097-4644
ISSN	0730-2312
DOI	10.1002/jcb.22965
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1114: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾

Article ; Online: Is there a role for autophagy in ascending aortopathy associated with tricuspid or bicuspid aortic valve?

Forte, Amalia / Cipollaro, Marilena / De Feo, Marisa / Della Corte, Alessandro

Clinical science (London, England : 1979)

2019 Volume 133, Issue 7, Page(s) 805–819

Abstract: Autophagy is a conserved process by which cytoplasmatic elements are sequestered in vesicles and degraded after their fusion with lysosomes, thus recycling the precursor molecules. The autophagy-mediated removal of redundant/harmful/damaged organelles ... ...

Abstract	Autophagy is a conserved process by which cytoplasmatic elements are sequestered in vesicles and degraded after their fusion with lysosomes, thus recycling the precursor molecules. The autophagy-mediated removal of redundant/harmful/damaged organelles and biomolecules plays not only a replenishing function, but protects against stressful conditions through an adaptive mechanism. Autophagy, known to play a role in several pathological conditions, is now gaining increasing attention also in the perspective of the identification of the pathogenetic mechanisms at the basis of ascending thoracic aortic aneurysm (TAA), a localized or diffused dilatation of the aorta with an abnormal widening greater than 50 percent of the vessel's normal diameter. TAA is less frequent than abdominal aortic aneurysm (AAA), but is encountered with a higher percentage in patients with congenital heart disease or known genetic syndromes. Several biological aspects of TAA pathophysiology remain to be elucitated and therapeutic needs are still widely unmet. One of the most controversial and epidemiologically important forms of TAA is that associated with the congenital bicuspid malformation of the aortic valve (BAV). Dysregulated autophagy in response, for example, to wall shear stress alterations, has been demonstrated to affect the phenotype of vascular cells relevant to aortopathy, with potential consequences on signaling, remodeling, and angiogenesis. The most recent findings and hypotheses concerning the multiple aspects of autophagy and of its dysregulation are summarized, both in general and in the context of the different vascular cell types and of TAA progression, with particular reference to BAV-related aortopathy.
MeSH term(s)	Animals ; Aorta, Thoracic/metabolism ; Aorta, Thoracic/pathology ; Aortic Aneurysm, Thoracic/etiology ; Aortic Aneurysm, Thoracic/metabolism ; Aortic Aneurysm, Thoracic/pathology ; Aortic Valve/abnormalities ; Aortic Valve/metabolism ; Aortic Valve/pathology ; Autophagy ; Autophagy-Related Proteins/metabolism ; Bicuspid Aortic Valve Disease ; Dilatation, Pathologic ; Heart Valve Diseases/complications ; Heart Valve Diseases/metabolism ; Heart Valve Diseases/pathology ; Humans ; Prognosis ; Risk Factors ; Signal Transduction
Chemical Substances	Autophagy-Related Proteins
Language	English
Publishing date	2019-04-02
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	206835-7
ISSN	1470-8736 ; 0301-0538 ; 0009-0360 ; 0143-5221
ISSN (online)	1470-8736
ISSN	0301-0538 ; 0009-0360 ; 0143-5221
DOI	10.1042/CS20181092
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ua VI Zs.220: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Ascending aortas from heart donors and CABG patients are not equivalent as control in aortopathy studies.

Forte, Amalia / Bancone, Ciro / Cipollaro, Marilena / De Feo, Marisa / Della Corte, Alessandro

Scandinavian cardiovascular journal : SCJ

2018 Volume 52, Issue 5, Page(s) 281–286

Abstract: Objectives: A careful selection of reference samples in studies on the pathogenesis of thoracic ascending aorta (TAA) dilation is crucial for reliability, consistency and reproducibility of experimental results. Several studies include control TAA ... ...

Abstract	Objectives: A careful selection of reference samples in studies on the pathogenesis of thoracic ascending aorta (TAA) dilation is crucial for reliability, consistency and reproducibility of experimental results. Several studies include control TAA samples from heart donors. Others include samples harvested during coronary artery bypass graft (CABG) procedures or a mix of samples from heart donors and CABG patients. We verified the equivalence/homogeneity of TAA samples from heart donors and CABG patients in terms of basal gene expression and thus their reliability as reference groups in aortopathy studies. Design: We analysed by RT-PCR and Western blot the differential expression of smoothelin, α-smooth muscle actin (α-SMA) and transforming growth factor-β1 (TGF-β1), selected as major players in smooth muscle cell and myofibroblast phenotype and remodelling. The mean age and comorbidities of subjects were consistent with data routinely seen in clinical practice. Results: Data revealed the loss of smoothelin in samples from CABG patients, together with a significant increase of α-SMA, while TGF-β1 dimer showed a marked increase in CABG patients versus heart donors, accompanied by a decrease of the corresponding mRNA. Differences in gene expression were maintained after adjustment for age. However, TGF-β1 mRNA and CABG patients' age showed a positive correlation (ρ = 0.89, p < .05), while α-SMA mRNA and age showed a negative correlation (ρ = -0.85, p < .05). Conclusions: We revealed the non-equivalence of samples from heart donors and CABG patients, presumably for the presence of microscopic atherosclerotic lesions in CABG patients, suggesting the necessity of a careful selection of control groups in aortopathy studies.
MeSH term(s)	Actins/analysis ; Actins/genetics ; Adult ; Aged ; Aorta, Thoracic/chemistry ; Aorta, Thoracic/pathology ; Aorta, Thoracic/surgery ; Aortic Diseases/metabolism ; Aortic Diseases/pathology ; Biomarkers/analysis ; Case-Control Studies ; Coronary Artery Bypass ; Cytoskeletal Proteins/analysis ; Cytoskeletal Proteins/genetics ; Female ; Heart Transplantation ; Humans ; Male ; Middle Aged ; Muscle Proteins/analysis ; Muscle Proteins/genetics ; Tissue Donors ; Tissue and Organ Harvesting/methods ; Transforming Growth Factor beta1/analysis ; Transforming Growth Factor beta1/genetics
Chemical Substances	ACTA2 protein, human ; Actins ; Biomarkers ; Cytoskeletal Proteins ; Muscle Proteins ; SMTN protein, human ; TGFB1 protein, human ; Transforming Growth Factor beta1
Language	English
Publishing date	2018-07-25
Publishing country	England
Document type	Comparative Study ; Journal Article
ZDB-ID	1379906-x
ISSN	1651-2006 ; 1401-7431
ISSN (online)	1651-2006
ISSN	1401-7431
DOI	10.1080/14017431.2018.1494303
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 638: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Genetic, epigenetic and stem cell alterations in endometriosis: new insights and potential therapeutic perspectives.

Forte, Amalia / Cipollaro, Marilena / Galderisi, Umberto

Clinical science (London, England : 1979)

2014 Volume 126, Issue 2, Page(s) 123–138

Abstract: Human endometrium is a highly dynamic tissue, undergoing periodic growth and regression at each menstrual cycle. Endometriosis is a frequent chronic pathological status characterized by endometrial tissue with an ectopic localization, causing pelvic pain ...

Abstract	Human endometrium is a highly dynamic tissue, undergoing periodic growth and regression at each menstrual cycle. Endometriosis is a frequent chronic pathological status characterized by endometrial tissue with an ectopic localization, causing pelvic pain and infertility and a variable clinical presentation. In addition, there is well-established evidence that, although endometriosis is considered benign, it is associated with an increased risk of malignant transformation in approximately 1.0% of affected women, with the involvement of multiple pathways of development. Increasing evidence supports a key contribution of different stem/progenitor cell populations not only in the cyclic regeneration of eutopic endometrium, but also in the pathogenesis of at least some types of endometriosis. Evidence has arisen from experiments in animal models of disease through different kinds of assays (including clonogenicity, the label-retaining cell approach, the analysis of undifferentiation markers), as well as from descriptive studies on ectopic and eutopic tissue samples harvested from affected women. Changes in stem cell populations in endometriotic lesions are associated with genetic and epigenetic alterations, including imbalance of miRNA expression, histone and DNA modifications and chromosomal aberrations. The present short review mainly summarizes the latest observations contributing to the current knowledge regarding the presence and the potential contribution of stem/progenitor cells in eutopic endometrium and the aetiology of endometriosis, together with a report of the most recently identified genetic and epigenetic alterations in endometriosis. We also describe the potential advantages of single cell molecular profiling in endometrium and in endometriotic lesions. All these data can have clinical implications and provide a basis for new potential therapeutic applications.
MeSH term(s)	Animals ; DNA/metabolism ; Down-Regulation ; Endometriosis/complications ; Endometriosis/genetics ; Endometriosis/pathology ; Endometrium/cytology ; Endometrium/metabolism ; Endometrium/pathology ; Epigenesis, Genetic/physiology ; Female ; Histones/metabolism ; Humans ; Menstruation/blood ; MicroRNAs/metabolism ; Ovarian Neoplasms/etiology ; RNA, Messenger/metabolism ; Stem Cells/metabolism ; Stem Cells/pathology ; Up-Regulation
Chemical Substances	Histones ; MicroRNAs ; RNA, Messenger ; DNA (9007-49-2)
Language	English
Publishing date	2014-01
Publishing country	England
Document type	Journal Article
ZDB-ID	206835-7
ISSN	1470-8736 ; 0301-0538 ; 0009-0360 ; 0143-5221
ISSN (online)	1470-8736
ISSN	0301-0538 ; 0009-0360 ; 0143-5221
DOI	10.1042/CS20130099
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ua VI Zs.220: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Locally different proteome in aortas from patients with stenotic tricuspid and bicuspid aortic valves†.

Forte, Amalia / Yin, Xiaoke / Fava, Marika / Bancone, Ciro / Cipollaro, Marilena / De Feo, Marisa / Mayr, Manuel / Jahangiri, Marjan / Della Corte, Alessandro

European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery

2019 Volume 56, Issue 3, Page(s) 458–469

Abstract: Objectives: We aimed to compare the intracellular proteome of ascending aortas from patients with stenotic bicuspid (BAV) and tricuspid aortic valves (TAV) to identify BAV-specific pathogenetic mechanisms of aortopathy and to verify the previously ... ...

Abstract	Objectives: We aimed to compare the intracellular proteome of ascending aortas from patients with stenotic bicuspid (BAV) and tricuspid aortic valves (TAV) to identify BAV-specific pathogenetic mechanisms of aortopathy and to verify the previously reported asymmetric expression of BAV aortopathy [concentrated at the convexity (CVX)] in its 'ascending phenotype' form. Methods: Samples were collected from the CVX and concavity sides of non-aneurysmal ascending aortas in 26 TAV and 26 BAV patients undergoing stenotic aortic valve replacement. Aortic lysates were subjected to cellular protein enrichment by subfractionation, and to proteome comparison by 2-dimensional fluorescence difference in-gel electrophoresis. Differentially regulated protein spots were identified by liquid chromatography-tandem mass spectrometry and analysed in silico. Selected results were verified by immunofluorescence and reverse transcription-polymerase chain reaction. Results: In BAV samples, 52 protein spots were differentially regulated versus TAV samples at the CVX and 10 spots at the concavity: liquid chromatography-tandem mass spectrometry identified 35 and 10 differentially regulated proteins, respectively. Charge trains of individual proteins (e.g. annexins) suggested the presence of post-translational modifications possibly modulating their activity. At the CVX, 37 of the 52 different protein spots showed decreased expression in BAV versus TAV. The affected biological pathways included those involved in smooth muscle cell contractile phenotype, metabolism and cell stress. Conclusions: The observed differential proteomics profiles may have a significant impact on the pathogenesis of the aortopathy, pointing the way for further studies. At a preaneurysmal stage, an aorta with BAV shows more protein expression changes and potentially more post-translational modifications at the CVX of the ascending aorta than at the concavity, compared to that of TAV.
MeSH term(s)	Aged ; Aorta/metabolism ; Aortic Valve/abnormalities ; Aortic Valve/metabolism ; Aortic Valve Stenosis/metabolism ; Bicuspid Aortic Valve Disease ; Electrophoresis, Gel, Two-Dimensional ; Female ; Heart Valve Diseases/metabolism ; Humans ; Male ; Microscopy, Fluorescence ; Phenotype ; Proteome/metabolism ; RNA/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Specimen Handling ; Tricuspid Valve/metabolism
Chemical Substances	Proteome ; RNA (63231-63-0)
Language	English
Publishing date	2019-02-22
Publishing country	Germany
Document type	Comparative Study ; Journal Article
ZDB-ID	639293-3
ISSN	1873-734X ; 1010-7940 ; 1567-4258
ISSN (online)	1873-734X
ISSN	1010-7940 ; 1567-4258
DOI	10.1093/ejcts/ezz032
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2303: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Epigenetic regulation of TGF-β1 signalling in dilative aortopathy of the thoracic ascending aorta.

Forte, Amalia / Galderisi, Umberto / Cipollaro, Marilena / De Feo, Marisa / Della Corte, Alessandro

Clinical science (London, England : 1979)

2016 Volume 130, Issue 16, Page(s) 1389–1405

Abstract: The term 'epigenetics' refers to heritable, reversible DNA or histone modifications that affect gene expression without modifying the DNA sequence. Epigenetic modulation of gene expression also includes the RNA interference mechanism. Epigenetic ... ...

Abstract	The term 'epigenetics' refers to heritable, reversible DNA or histone modifications that affect gene expression without modifying the DNA sequence. Epigenetic modulation of gene expression also includes the RNA interference mechanism. Epigenetic regulation of gene expression is fundamental during development and throughout life, also playing a central role in disease progression. The transforming growth factor β1 (TGF-β1) and its downstream effectors are key players in tissue repair and fibrosis, extracellular matrix remodelling, inflammation, cell proliferation and migration. TGF-β1 can also induce cell switch in epithelial-to-mesenchymal transition, leading to myofibroblast transdifferentiation. Cellular pathways triggered by TGF-β1 in thoracic ascending aorta dilatation have relevant roles to play in remodelling of the vascular wall by virtue of their association with monogenic syndromes that implicate an aortic aneurysm, including Loeys-Dietz and Marfan's syndromes. Several studies and reviews have focused on the progression of aneurysms in the abdominal aorta, but research efforts are now increasingly being focused on pathogenic mechanisms of thoracic ascending aorta dilatation. The present review summarizes the most recent findings concerning the epigenetic regulation of effectors of TGF-β1 pathways, triggered by sporadic dilative aortopathy of the thoracic ascending aorta in the presence of a tricuspid or bicuspid aortic valve, a congenital malformation occurring in 0.5-2% of the general population. A more in-depth comprehension of the epigenetic alterations associated with TGF-β1 canonical and non-canonical pathways in dilatation of the ascending aorta could be helpful to clarify its pathogenesis, identify early potential biomarkers of disease, and, possibly, develop preventive and therapeutic strategies.
MeSH term(s)	Animals ; Aorta, Thoracic/metabolism ; Aortic Diseases/genetics ; Aortic Diseases/metabolism ; Epigenesis, Genetic ; Humans ; Signal Transduction ; Transforming Growth Factor beta1/genetics ; Transforming Growth Factor beta1/metabolism
Chemical Substances	Transforming Growth Factor beta1
Language	English
Publishing date	2016-07-07
Publishing country	England
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	206835-7
ISSN	1470-8736 ; 0301-0538 ; 0009-0360 ; 0143-5221
ISSN (online)	1470-8736
ISSN	0301-0538 ; 0009-0360 ; 0143-5221
DOI	10.1042/CS20160222
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ua VI Zs.220: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Author Correction: Pro-inflammatory cytokines activate hypoxia-inducible factor 3α via epigenetic changes in mesenchymal stromal/stem cells.

Cuomo, Francesca / Coppola, Antonietta / Botti, Chiara / Maione, Ciro / Forte, Amalia / Scisciola, Lucia / Liguori, Giuseppina / Caiafa, Ilaria / Ursini, Matilde Valeria / Galderisi, Umberto / Cipollaro, Marilena / Altucci, Lucia / Cobellis, Gilda

Scientific reports

2020 Volume 10, Issue 1, Page(s) 6776

Abstract: An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

Abstract	An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Language	English
Publishing date	2020-04-17
Publishing country	England
Document type	Published Erratum
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-020-62861-8
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Chromatin modification and senescence.

Di Bernardo, Giovanni / Cipollaro, Marilena / Galderisi, Umberto

Current pharmaceutical design

2011 Volume 18, Issue 13, Page(s) 1686–1693

Abstract: Cells are the fundamental structure composing our bodies and hence cellular decline (called senescence) contributes to ageing. Endogenous and exogenous stresses may induce cellular senescence. Stressors are mainly macromolecule damage events, which ... ...

Abstract	Cells are the fundamental structure composing our bodies and hence cellular decline (called senescence) contributes to ageing. Endogenous and exogenous stresses may induce cellular senescence. Stressors are mainly macromolecule damage events, which include: shortening of chromosome telomeres; non-telomeric DNA damage; excessive mitogenic signals, which may cause DNA damage; and non-genotoxic stress, such as perturbations to chromatin organization. For many years the analysis of chromatin perturbation as a leading event in triggering senescence has been overlooked. Now, it is well recognized that chromatin DNA packaging is not immune to the ravages of time. All eukaryotes experience changes in chromatin organization and gene-expression patterns as they age. This can be due to perturbation in the function of chromatin modifiers. In this review we will discuss the role in the senescence process of the different types of chromatin modifiers, such as the ATP-dependent chromatin remodelling complexes, the enzymes that covalently modify histone tails and proteins involved in DNA methylation.
MeSH term(s)	Animals ; Cellular Senescence ; Chromatin Assembly and Disassembly ; Humans
Language	English
Publishing date	2011-08-04
Publishing country	United Arab Emirates
Document type	Journal Article ; Review
ZDB-ID	1304236-1
ISSN	1873-4286 ; 1381-6128
ISSN (online)	1873-4286
ISSN	1381-6128
DOI	10.2174/138161212799859693
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 4714: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Impact of lysosomal storage disorders on biology of mesenchymal stem cells: Evidences from in vitro silencing of glucocerebrosidase (GBA) and alpha-galactosidase A (GLA) enzymes.

Squillaro, Tiziana / Antonucci, Ivana / Alessio, Nicola / Esposito, Anna / Cipollaro, Marilena / Melone, Mariarosa Anna Beatrice / Peluso, Gianfranco / Stuppia, Liborio / Galderisi, Umberto

Journal of cellular physiology

2017 Volume 232, Issue 12, Page(s) 3454–3467

Abstract: Lysosomal storage disorders (LDS) comprise a group of rare multisystemic diseases resulting from inherited gene mutations that impair lysosomal homeostasis. The most common LSDs, Gaucher disease (GD), and Fabry disease (FD) are caused by deficiencies in ... ...

Abstract	Lysosomal storage disorders (LDS) comprise a group of rare multisystemic diseases resulting from inherited gene mutations that impair lysosomal homeostasis. The most common LSDs, Gaucher disease (GD), and Fabry disease (FD) are caused by deficiencies in the lysosomal glucocerebrosidase (GBA) and alpha-galactosidase A (GLA) enzymes, respectively. Given the systemic nature of enzyme deficiency, we hypothesized that the stem cell compartment of GD and FD patients might be also affected. Among stem cells, mesenchymal stem cells (MSCs) are a commonly investigated population given their role in hematopoiesis and the homeostatic maintenance of many organs and tissues. Since the impairment of MSC functions could pose profound consequences on body physiology, we evaluated whether GBA and GLA silencing could affect the biology of MSCs isolated from bone marrow and amniotic fluid. Those cell populations were chosen given the former's key role in organ physiology and the latter's intriguing potential as an alternative stem cell model for human genetic disease. Our results revealed that GBA and GLA deficiencies prompted cell cycle arrest along with the impairment of autophagic flux and an increase of apoptotic and senescent cell percentages. Moreover, an increase in ataxia-telangiectasia-mutated staining 1 hr after oxidative stress induction and a return to basal level at 48 hr, along with persistent gamma-H2AX staining, indicated that MSCs properly activated DNA repair signaling, though some damages remained unrepaired. Our data therefore suggest that MSCs with reduced GBA or GLA activity are prone to apoptosis and senescence due to impaired autophagy and DNA repair capacity.
MeSH term(s)	Amniotic Fluid/cytology ; Apoptosis ; Autophagy ; Bone Marrow Cells/enzymology ; Bone Marrow Cells/pathology ; Cell Separation ; Cells, Cultured ; Cellular Senescence ; Child ; DNA Repair ; Fabry Disease/enzymology ; Fabry Disease/genetics ; Fabry Disease/pathology ; Female ; Gaucher Disease/enzymology ; Gaucher Disease/genetics ; Gaucher Disease/pathology ; Glucosylceramidase/deficiency ; Glucosylceramidase/genetics ; Humans ; Mesenchymal Stem Cells/enzymology ; Mesenchymal Stem Cells/pathology ; RNA Interference ; Retinoblastoma Protein/genetics ; Retinoblastoma Protein/metabolism ; S Phase Cell Cycle Checkpoints ; Signal Transduction ; Stem Cell Niche ; Transfection ; Tumor Suppressor Protein p53/genetics ; Tumor Suppressor Protein p53/metabolism ; alpha-Galactosidase/genetics ; alpha-Galactosidase/metabolism
Chemical Substances	Retinoblastoma Protein ; TP53 protein, human ; Tumor Suppressor Protein p53 ; GLA protein, human (EC 3.2.1.22) ; alpha-Galactosidase (EC 3.2.1.22) ; Glucosylceramidase (EC 3.2.1.45)
Language	English
Publishing date	2017-02-07
Publishing country	United States
Document type	Journal Article
ZDB-ID	3116-1
ISSN	1097-4652 ; 0021-9541
ISSN (online)	1097-4652
ISSN	0021-9541
DOI	10.1002/jcp.25807
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Uc I Zs.212: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Misidentified Human Gene Functions with Mouse Models: The Case of the Retinoblastoma Gene Family in Senescence.

Alessio, Nicola / Capasso, Stefania / Ferone, Angela / Di Bernardo, Giovanni / Cipollaro, Marilena / Casale, Fiorina / Peluso, Gianfranco / Giordano, Antonio / Galderisi, Umberto

Neoplasia (New York, N.Y.)

2017 Volume 19, Issue 10, Page(s) 781–790

Abstract: Although mice models rank among the most widely used tools for understanding human genetics, biology, and diseases, differences between orthologous genes among species as close as mammals are possible, particularly in orthologous gene pairs in which one ... ...

Abstract	Although mice models rank among the most widely used tools for understanding human genetics, biology, and diseases, differences between orthologous genes among species as close as mammals are possible, particularly in orthologous gene pairs in which one or more paralogous (i.e., duplicated) genes appear in the genomes of the species. Duplicated genes can possess overlapping functions and compensate for each other. The retinoblastoma gene family demonstrates typical composite functionality in its three member genes (i.e., RB1, RB2/P130, and P107), all of which participate in controlling the cell cycle and associated phenomena, including proliferation, quiescence, apoptosis, senescence, and cell differentiation. We analyzed the role of the retinoblastoma gene family in regulating senescence in mice and humans. Silencing experiments with each member of the gene family in mesenchymal stromal cells (MSCs) and fibroblasts from mouse and human tissues demonstrated that RB1 may be indispensable for senescence in mouse cells, but not in human ones, as an example of species specificity. Furthermore, although RB2/P130 seems to be implicated in maintaining human cell senescence, the function of RB1 within any given species might differ by cell type, as an example of cell specificity. For instance, silencing RB1 in mouse fibroblasts induced a reduced senescence not observed in mouse MSCs. Our findings could be useful as a general paradigm of cautions to take when inferring the role of human genes analyzed in animal studies and when examining the role of the retinoblastoma gene family in detail.
Language	English
Publishing date	2017-10
Publishing country	United States
Document type	Journal Article
ZDB-ID	1483840-0
ISSN	1476-5586 ; 1522-8002
ISSN (online)	1476-5586
ISSN	1522-8002
DOI	10.1016/j.neo.2017.06.005
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 5203: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito