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Article ; Online: Correction: Evaluation of separation performance for eggshell-based reversed-phase HPLC columns by controlling particle size and application in quantitative therapeutic drug monitoring.

Yoshii, Tomoka / Nakano, Kohei / Okuda, Tomoaki / Citterio, Daniel / Hiruta, Yuki

Analytical methods : advancing methods and applications

2024 Volume 16, Issue 9, Page(s) 1416

Abstract: Correction for 'Evaluation of separation performance for eggshell-based reversed-phase HPLC columns by controlling particle size and application in quantitative therapeutic drug monitoring' by Tomoka ... ...

Abstract	Correction for 'Evaluation of separation performance for eggshell-based reversed-phase HPLC columns by controlling particle size and application in quantitative therapeutic drug monitoring' by Tomoka Yoshii
Language	English
Publishing date	2024-02-29
Publishing country	England
Document type	Published Erratum
ZDB-ID	2515210-5
ISSN	1759-9679 ; 1759-9660
ISSN (online)	1759-9679
ISSN	1759-9660
DOI	10.1039/d4ay90022g
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Evaluation of separation performance for eggshell-based reversed-phase HPLC columns by controlling particle size and application in quantitative therapeutic drug monitoring.

Yoshii, Tomoka / Nakano, Kohei / Okuda, Tomoaki / Citterio, Daniel / Hiruta, Yuki

Analytical methods : advancing methods and applications

2023 Volume 15, Issue 14, Page(s) 1790–1796

Abstract: Eggshell-based reversed-phase packing materials were applied to an analytical column for high-performance liquid chromatography. Commercially available eggshell powder was classified by a cyclone system to obtain three types of particles with different ... ...

Abstract	Eggshell-based reversed-phase packing materials were applied to an analytical column for high-performance liquid chromatography. Commercially available eggshell powder was classified by a cyclone system to obtain three types of particles with different diameters (arithmetic mean ± standard deviation: 4.3 ± 3.8, 5.6 ± 3.3, and 9.5 ± 5.5 μm). Sedimentation separation removed tiny particles from each sample, resulting in particles with arithmetic means of 6.6 ± 5.5, 7.3 ± 4.5, and 10.2 ± 5.0 μm, respectively. The unclassified particles and three particle types treated with sedimentation separation were subsequently packed into analytical columns (150 mm × 4.6 mm I.D.), and their separation efficiencies were evaluated by comparing their height equivalent to a theoretical plate (HETP). The column without sedimentation separation exhibited the highest HETP, whereas the columns with sedimentation separation showed better separation efficiency and lower back pressure. The column with the best separation efficiency was applied for the separation of 10 alkylbenzenes and 5 steroids, and all peaks were observed with complete separation (peak resolution:
MeSH term(s)	Animals ; Chromatography, High Pressure Liquid/methods ; Particle Size ; Drug Monitoring ; Egg Shell ; Porosity
Language	English
Publishing date	2023-04-06
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2515210-5
ISSN	1759-9679 ; 1759-9660
ISSN (online)	1759-9679
ISSN	1759-9660
DOI	10.1039/d3ay00219e
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Article ; Online: Evaluation of cellophane as platform for colorimetric assays on microfluidic analytical devices.

Shigemori, Hiroki / Maejima, Kento / Shibata, Hiroyuki / Hiruta, Yuki / Citterio, Daniel

Mikrochimica acta

2023 Volume 190, Issue 2, Page(s) 48

Abstract: Due to their low cost, simplicity, and pump-free liquid transport properties, colorimetric assays on paper spots and microfluidic paper-based analytical devices (µPADs) are regarded as useful tools for point-of-care testing (POCT). However, for certain ... ...

Abstract	Due to their low cost, simplicity, and pump-free liquid transport properties, colorimetric assays on paper spots and microfluidic paper-based analytical devices (µPADs) are regarded as useful tools for point-of-care testing (POCT). However, for certain types of colorimetric assays, the "non-transparent" and "white" characters of paper can be a disadvantage. In this work, the possibilities of using cellophane as an alternative platform for colorimetric assays have been investigated. Cellophane is a low cost and easy-to-handle transparent film made of regenerated cellulose. Owing to its hydrophilic character, cellophane-based microfluidic channels fabricated through a print-cut-laminate approach enabled pump-free liquid transport into multiple detection areas, similar to µPADs. In addition, the water absorption characteristics of cellophane allowed the stable immobilization of water-soluble colorimetric indicators without any surface modification or additional reagents. The transparency of cellophane provides possibilities for simple background coloring of the substrates, increasing the dynamic signal range for hue-based colorimetric assays, as demonstrated for two model assays targeting H
MeSH term(s)	Humans ; Microfluidics ; Microfluidic Analytical Techniques ; Cellophane ; Colorimetry ; Creatinine ; Hydrogen Peroxide ; Water
Chemical Substances	Cellophane (9005-81-6) ; Creatinine (AYI8EX34EU) ; Hydrogen Peroxide (BBX060AN9V) ; Water (059QF0KO0R)
Language	English
Publishing date	2023-01-09
Publishing country	Austria
Document type	Journal Article
ZDB-ID	89-9
ISSN	1436-5073 ; 0026-3672
ISSN (online)	1436-5073
ISSN	0026-3672
DOI	10.1007/s00604-022-05622-w
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Article ; Online: Improvement in sensitivity for lateral flow immunoassay of ferritin using novel device design based on gold-enhanced gold nanoparticles.

Preechakasedkit, Pattarachaya / Teekayupak, Kanyapat / Citterio, Daniel / Ruecha, Nipapan

Scientific reports

2022 Volume 12, Issue 1, Page(s) 7831

Abstract: This work introduces a low-cost adhesive tape combined with a hydroxylamine/polyvinyl alcohol/polyethylene oxide (HA/PVA/PEO) blend film to fabricate novel devices for improving sensitivity of gold nanoparticle (AuNP)-based lateral flow immunoassays ( ... ...

Abstract	This work introduces a low-cost adhesive tape combined with a hydroxylamine/polyvinyl alcohol/polyethylene oxide (HA/PVA/PEO) blend film to fabricate novel devices for improving sensitivity of gold nanoparticle (AuNP)-based lateral flow immunoassays (LFIAs) via two platforms: (1) LFIA device with integrated gold enhancement and (2) LFIA device with two independent sample inlets. The detection of ferritin has been used for proof-of-concept. The adhesive tape inserted in the devices assists to separate two solutions independently flowing from two different inlets toward a nitrocellulose membrane. On-device gold enhancement was achieved by the enlargement of AuNPs via the catalytic reaction of KAuCl
MeSH term(s)	Equipment Design ; Ferritins ; Gold ; Humans ; Immunoassay/methods ; Limit of Detection ; Metal Nanoparticles
Chemical Substances	Gold (7440-57-5) ; Ferritins (9007-73-2)
Language	English
Publishing date	2022-05-12
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-022-11732-5
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Article ; Online: Traffic light type paper-based analytical device for intuitive and semi-quantitative naked-eye signal readout.

Ohta, Sera / Hiraoka, Ryuya / Hiruta, Yuki / Citterio, Daniel

Lab on a chip

2022 Volume 22, Issue 4, Page(s) 717–726

Abstract: Microfluidic paper-based analytical devices (μPADs) have attracted great attention as potential candidates for point-of-care testing (POCT). Nevertheless, only a limited number of μPADs expected to satisfy the standard of Clinical Laboratory Improvement ... ...

Abstract	Microfluidic paper-based analytical devices (μPADs) have attracted great attention as potential candidates for point-of-care testing (POCT). Nevertheless, only a limited number of μPADs expected to satisfy the standard of Clinical Laboratory Improvement Amendments (CLIA) waived tests as issued by the US Food and Drug Administration (FDA) have been reported. This work introduces a "traffic light type μPAD", enabling highly intuitive semi-quantitative equipment-free naked-eye readout with no need for calibration, subjective interpretation or calculation. Assay results are displayed as traffic light colours reporting 5 analyte concentration levels (green/green & yellow/yellow/yellow & red/red). The device has been designed to never display all three colours simultaneously, eliminating any risk for misinterpretation. The mechanism relies on the modulation of sample flow through a network of paperfluidic channels modified with a hydrophobic to hydrophilic phase-switching substance responsive to H
MeSH term(s)	Hydrogen Peroxide ; Lab-On-A-Chip Devices ; Microfluidic Analytical Techniques ; Paper ; Point-of-Care Testing
Chemical Substances	Hydrogen Peroxide (BBX060AN9V)
Language	English
Publishing date	2022-02-15
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2056646-3
ISSN	1473-0189 ; 1473-0197
ISSN (online)	1473-0189
ISSN	1473-0197
DOI	10.1039/d1lc01060c
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Article ; Online: Quantitative evaluation of reversed-phase packing material based on calcium carbonate microspheres modified with an alternating copolymer.

Kaizu, Kogi / Mochida, Mai / Imai, Hiroaki / Citterio, Daniel / Hiruta, Yuki

Journal of chromatography. A

2022 Volume 1677, Page(s) 463294

Abstract: Considering the vulnerability of silica gel to alkaline mobile phases, a highly alkaline stable stationary phase for HPLC is required to separate basic compounds with high separation efficiency. To address this issue, we have developed a high alkaline ... ...

Abstract	Considering the vulnerability of silica gel to alkaline mobile phases, a highly alkaline stable stationary phase for HPLC is required to separate basic compounds with high separation efficiency. To address this issue, we have developed a high alkaline stable packing material (CaCO
MeSH term(s)	Calcium Carbonate ; Chromatography, High Pressure Liquid/methods ; Chromatography, Reverse-Phase/methods ; Hydrophobic and Hydrophilic Interactions ; Microspheres ; Polymers/chemistry ; Silica Gel/chemistry ; Silicon Dioxide/chemistry
Chemical Substances	Polymers ; Silica Gel (60650-90-0) ; Silicon Dioxide (7631-86-9) ; Calcium Carbonate (H0G9379FGK)
Language	English
Publishing date	2022-06-30
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1171488-8
ISSN	1873-3778 ; 0021-9673
ISSN (online)	1873-3778
ISSN	0021-9673
DOI	10.1016/j.chroma.2022.463294
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Article ; Online: Dual Colorimetric/Electrochemical Detection of

Preechakasedkit, Pattarachaya / Panphut, Wattana / Lomae, Atchara / Wonsawat, Wanida / Citterio, Daniel / Ruecha, Nipapan

Analytical chemistry

2023 Volume 95, Issue 37, Page(s) 13904–13912

Abstract: Foodborne illnesses caused by the ingestion of contaminated foods or beverages are a serious concern due to the millions of reported cases per year. It is essential to develop sensitive and rapid detection methods of foodborne pathogens to ensure food ... ...

Abstract	Foodborne illnesses caused by the ingestion of contaminated foods or beverages are a serious concern due to the millions of reported cases per year. It is essential to develop sensitive and rapid detection methods of foodborne pathogens to ensure food safety for producers and consumers. Unfortunately, current detection techniques still suffer from time-consuming operations and the need for highly skilled personnel. Here, we introduce a highly sensitive dual colorimetric/electrochemical detection approach for
MeSH term(s)	Colorimetry ; Gold ; Graphite ; Metal Nanoparticles ; Salmonella typhimurium ; Silver ; Immunoassay ; Lasers
Chemical Substances	Gold (7440-57-5) ; Graphite (7782-42-5) ; Silver (3M4G523W1G)
Language	English
Publishing date	2023-08-28
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.3c02252
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Article ; Online: Simplified capture, extraction, and amplification of cellular DNA from water samples.

Kawaguchi, Mai / Aoki, Hiroshi / Kamo, Hiroki / Miura, Kazuki / Hiruta, Yuki / Simizu, Siro / Citterio, Daniel

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

2023 Volume 40, Issue 3, Page(s) 501–510

Abstract: DNA analysis in water samples is attracting attention in various fields. However, conventional methods for DNA analysis require a work-intensive and time-consuming sample pre-treatment. In this study, a simplified pre-treatment method for analyzing DNA ... ...

Abstract	DNA analysis in water samples is attracting attention in various fields. However, conventional methods for DNA analysis require a work-intensive and time-consuming sample pre-treatment. In this study, a simplified pre-treatment method for analyzing DNA in water samples was evaluated. The process consists of filtration, DNA extraction, and amplification, which can be achieved within a short time. In the filtration process, two types of filters, firstly a tissue paper (Kimwipe) and then a glass filter (GF/F), were used in sequence. The first large pore size filter enabled a reduction in filtration time by removing large particulate matter impurities present in river water matrix. Cells spiked into 1 L of river water were recovered at more than 90% within approximately 5 min filtration time. Also, DNA was extracted from the captured cells directly on the surface of the filter in only 5 min. Thus, DNA collection and extraction from a water sample can be completed within about 10 min. Furthermore, PCR amplification was performed directly from DNA-attached filter sections, which greatly reduced the number of required pre-treatment steps. Finally, we succeeded in establishing a simple and fast on-site pre-treatment system by using a hand-driven syringe filtration method. This pre-treatment system is expected to offer the possibility for the future establishment of a rapid and easy DNA analysis method applicable to various types of water samples.
MeSH term(s)	DNA/genetics ; Fresh Water ; Filtration/methods ; Water
Chemical Substances	DNA (9007-49-2) ; Water (059QF0KO0R)
Language	English
Publishing date	2023-12-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1483376-1
ISSN	1348-2246 ; 1348-2246
ISSN (online)	1348-2246
ISSN	1348-2246
DOI	10.1007/s44211-023-00482-7
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Article ; Online: All-printed semiquantitative paper-based analytical devices relying on QR code array readout.

Katoh, Aya / Maejima, Kento / Hiruta, Yuki / Citterio, Daniel

The Analyst

2020 Volume 145, Issue 18, Page(s) 6071–6078

Abstract: The wide spread of smartphones and QR codes for various end-user applications has had an impact beyond traditional fields of use, recently also reaching point-of-care testing (POCT). This work presents the integration of QR code recognition into paper- ... ...

Abstract	The wide spread of smartphones and QR codes for various end-user applications has had an impact beyond traditional fields of use, recently also reaching point-of-care testing (POCT). This work presents the integration of QR code recognition into paper-based analytical devices (PADs) with "distance-based" colorimetric signalling, resulting in semiquantitative readout fully relying on straightforward barcode reader solutions. PADs consist of an array of QR codes arranged in series inside a paperfluidic channel. A mask dye concept has been developed, which enables utilisation of colour changing indicators by initially hiding QR codes. The colour change of the indicator induced by the presence of an analyte of interest results in gradual unmasking of QR codes, which become recognisable by the smartphone barcode reader app. To reproducibly fabricate devices, all fabrication steps were performed by commercial desktop solid ink and inkjet printing. The QR code masking function was optimised by controlling the amount of printed mask dye through adjusting the opacity of printing patterns during the inkjet deposition process. For proof-of-concept, a model assay in the form of colorimetric copper ion (Cu2+) detection in the concentration range of 0.4 mM to 3.2 mM was evaluated. Consistent results independent of the smartphone model and environmental light condition were achieved with a free barcode reader app. To the best of our knowledge, this work is the first demonstration of a semiquantitative assay approach fully relying on QR code readout without digital colour analysis, customised app or hardware modification.
MeSH term(s)	Colorimetry ; Smartphone
Language	English
Publishing date	2020-08-19
Publishing country	England
Document type	Journal Article
ZDB-ID	210747-8
ISSN	1364-5528 ; 0003-2654
ISSN (online)	1364-5528
ISSN	0003-2654
DOI	10.1039/d0an00955e
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Article ; Online: Centrifugal Paperfluidic Platform for Accelerated Distance-Based Colorimetric Signal Readout.

Maejima, Kento / Hiruta, Yuki / Citterio, Daniel

Analytical chemistry

2020 Volume 92, Issue 7, Page(s) 4749–4754

Abstract: Distance-based readout is one of the most user-friendly and simple colorimetric signaling methods widely applied for paper-based analytical devices (PADs). This work presents the integration of distance readout PADs into a centrifugal platform enabling ... ...

Abstract	Distance-based readout is one of the most user-friendly and simple colorimetric signaling methods widely applied for paper-based analytical devices (PADs). This work presents the integration of distance readout PADs into a centrifugal platform enabling affordable, rapid, and sample volume-independent colorimetric assays. Centrifugally assisted distance-based PADs (CD-PADs) eliminate the requirement to use micropipets for sample introduction and reduce the overall time for distance-based assays. All device fabrication steps were performed through computer-controlled print, cut, and laminate (PCL) techniques oriented toward mass production. The inexpensive centrifugal platform was built on a recycled DVD player combined with an open source microcomputer (Arduino). Assay protocols, including rotational velocity and rotation time, were optimized to obtain a maximum dynamic range and reproducible results for sample volume metering (coefficient of variation 3.62%). A colorimetric Ni
Language	English
Publishing date	2020-03-24
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.9b05782
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