LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 3 of total 3

Search options

  1. Article ; Online: Fluorescent nanodiamond labels

    Claudia Reyes San-Martin / Yue Zhang / Thamir Hamoh / Lotte Berendse / Carline Klijn / Runrun Li / Alina Sigaeva / Jakub Kawałko / Hui Ting Li / Jian Tehrani / Aldona Mzyk / Romana Schirhagl

    Materials Today Bio, Vol 20, Iss , Pp 100629- (2023)

    Size and concentration matters for sperm cell viability

    2023  

    Abstract: Nanodiamonds are increasingly popular in biomedical applications, including optical labelling, drug delivery and nanoscale sensing. Potential new applications are in studying infertility or labelling sperm cells. However, for these applications, it is ... ...

    Abstract Nanodiamonds are increasingly popular in biomedical applications, including optical labelling, drug delivery and nanoscale sensing. Potential new applications are in studying infertility or labelling sperm cells. However, for these applications, it is necessary that nanodiamonds are inert and do not alter sperm properties. In this article, we assessed the biocompatibility of nanodiamonds in detail. We investigated different sizes and concentrations of nanodiamonds and sperm preparation methods. We evaluated if the metabolic activity, membrane integrity, morphology and formation of reactive oxygen species were altered. These parameters were tested for sperm cells in their uncapacitated and capacitated states. Unfortunately, FNDs are not universally biocompatible. Generally, cells in the capacitated state are more prone to stress. Additionally, larger particles and lower concentrations are tolerated better than smaller and higher concentrated particles.
    Keywords Nanodiamonds ; NV centers ; Biocompatibility ; Sperm cells ; Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2023-06-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Article ; Online: Applying NV center-based quantum sensing to study intracellular free radical response upon viral infections

    Kaiqi Wu / Thea A. Vedelaar / Viraj G. Damle / Aryan Morita / Julie Mougnaud / Claudia Reyes San Martin / Yue Zhang / Denise P.I. van der Pol / Heidi Ende-Metselaar / Izabela Rodenhuis-Zybert / Romana Schirhagl

    Redox Biology, Vol 52, Iss , Pp 102279- (2022)

    2022  

    Abstract: Although viruses are known to modify the free radical concentration in infected cells, the exact location and concentrations of such changes remain unknown. Although this information is important to understand the virus pathogenesis and design better ... ...

    Abstract Although viruses are known to modify the free radical concentration in infected cells, the exact location and concentrations of such changes remain unknown. Although this information is important to understand the virus pathogenesis and design better anti-viral drugs or vaccines, obtaining it with the conventional free radical/ROS detection techniques is impossible. Here, we elucidate the utility of diamond magnetometry for studying the free radical response of baby hamster kidney-21 cells upon Semliki Forest virus infection. Specifically, we optically probe the alterations in free radical concentration near infectious viruses via measuring the spin–lattice relaxation (T1) of NV defect ensembles embedded in intracellular nanodiamonds. We performed measurements both at random locations as well as close to the virus entry by conjugating viruses to nanodiamond sensors. We observed alterations of T1, which represent the intracellular free radical concentration during the viral replication process. Moreover, relaxometry is also used to monitor real-time free radical variation during the early infectious process.
    Keywords Fluorescent nanodiamonds ; Free radicals ; Viral infections ; Diamond magnetometry ; NV centers ; ROS ; Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
    Subject code 500
    Language English
    Publishing date 2022-06-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article ; Online: Male subfertility and oxidative stress

    Emily P.P. Evans / Jorien T.M. Scholten / Aldona Mzyk / Claudia Reyes-San-Martin / Arturo E. Llumbet / Thamir Hamoh / Eus G.J.M. Arts / Romana Schirhagl / Astrid E.P. Cantineau

    Redox Biology, Vol 46, Iss , Pp 102071- (2021)

    2021  

    Abstract: To date 15% of couples are suffering from infertility with 45–50% of males being responsible. With an increase in paternal age as well as various environmental and lifestyle factors worsening these figures are expected to increase. As the so-called free ... ...

    Abstract To date 15% of couples are suffering from infertility with 45–50% of males being responsible. With an increase in paternal age as well as various environmental and lifestyle factors worsening these figures are expected to increase. As the so-called free radical theory of infertility suggests, free radicals or reactive oxygen species (ROS) play an essential role in this process. However, ROS also fulfill important functions for instance in sperm maturation. The aim of this review article is to discuss the role reactive oxygen species play in male fertility and how these are influenced by lifestyle, age or disease. We will further discuss how these ROS are measured and how they can be avoided during in-vitro fertilization.
    Keywords Reactive oxygen species ; Oxidative stress ; Infertility ; Subfertility ; Sperm ; Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2021-10-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top