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Article ; Online: Medial gastrocnemius rotational flap for the reconstruction of extensor mechanism disruption in periprosthetic knee infections: a retrospective series with minimum two year follow-up.

Russo, Antonio / Clemente, Antonio / Massè, Alessandro / Burastero, Giorgio

2023 Volume 47, Issue 4, Page(s) 983–993

Abstract: Purpose: Extensor mechanism disruption following total knee arthroplasty has a prevalence ranging from 0.3 to 3%. Its management is challenging, especially in case of associated infection of the prosthetic implant. Surgical options are limited due to ... ...

Abstract	Purpose: Extensor mechanism disruption following total knee arthroplasty has a prevalence ranging from 0.3 to 3%. Its management is challenging, especially in case of associated infection of the prosthetic implant. Surgical options are limited due to the septic process, and the use of allograft or synthetic mesh are not recommended. The aim of this study was to report clinical outcomes, complications, survival, and surgical technique of medial gastrocnemius flap for the treatment of extensor mechanism disruptions associated with periprosthetic knee infection. Methods: This is a retrospective study from a prospectively collected arthroplasty registry from 2012 to 2019. Patients who received the gastrocnemius flap in the setting of a two-stage knee replacement for periprosthetic infection were included. Results of physical examination, Knee Society Score, Oxford Knee Score, and measurement of the range of motion registered pre-operatively were compared to those obtained at last follow-up. Survival was analysed through Kaplan-Meier curve. Results: A total of 15 patients were included, with a mean age of 63.4 years (range 36-77). The reconstruction of the extensor mechanism demonstrated a success rate of 73.3%. The mean extension lag at final follow-up was 7.5° (range, 0-30). The mean Knee Society Score and Oxford Knee Score improved from 29.0 (range, 21-36) and 17.5 (range, 13-22) respectively, to 82.9 (range, 74-89) and 36.0 (range, 33-39). Conclusion: Medial gastrocnemius rotational flap is a reliable option for joint and limb salvage in case of periprosthetic knee infection associated with wide soft tissue degeneration and extensor mechanism disruption. The technique and surgical protocol presented in this study are reproducible and guaranteed good clinical outcomes and infection control.
MeSH term(s)	Humans ; Adult ; Middle Aged ; Aged ; Retrospective Studies ; Follow-Up Studies ; Treatment Outcome ; Muscle, Skeletal/surgery ; Knee Joint/surgery ; Arthroplasty, Replacement, Knee/adverse effects ; Arthroplasty, Replacement, Knee/methods ; Knee Prosthesis/adverse effects ; Reoperation
Language	English
Publishing date	2023-02-08
Publishing country	Germany
Document type	Journal Article
ZDB-ID	80384-4
ISSN	1432-5195 ; 0341-2695
ISSN (online)	1432-5195
ISSN	0341-2695
DOI	10.1007/s00264-023-05716-x
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Article ; Online: Le Covidor

Clemente, Antonio Alberto

2020

Abstract: Il mantenimento della distanza interpersonale di almeno un metro e l’obbligo di evitare abbracci e strette di mano è un’opera di distanziamento sociale, introdotta dalla legge, per contenere gli effetti nefasti del Covid-19. Un provvedimento emergenziale ...

Abstract	Il mantenimento della distanza interpersonale di almeno un metro e l’obbligo di evitare abbracci e strette di mano è un’opera di distanziamento sociale, introdotta dalla legge, per contenere gli effetti nefasti del Covid-19. Un provvedimento emergenziale che è stato, al contempo, una forte limitazione delle libertà personali, un cambiamento radicale del comportamento in pubblico, una mutazione del rituale di interazione. E che ha creato una condizione del tutto peculiare: l’uomo è diventato un’isola e la società un arcipelago in cui l’unico rapporto di reciprocità è stato quello visivo-sonoro. Sempre a condizione di rispettare la distanza di sicurezza interpersonale. Nato come soluzione temporanea, il distanziamento sociale continuerà a essere praticato, volontariamente, in ogni città, anche dopo la fine della fase emergenziale.
Keywords	Distanziamento sociale ; contagio ; abitare ; sconfinamento ; covid19
Language	Italian
Publisher	Lettera ventidue
Publishing country	it
Document type	Article ; Online
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Article ; Online: Design of a binary programmable transmitarray based on phase change material for beam steering applications in D-band.

Gharbieh, Samara / Milbrandt, Jorick / Reig, Bruno / Mercier, Denis / Allain, Marjolaine / Clemente, Antonio

Scientific reports

2024 Volume 14, Issue 1, Page(s) 2966

Abstract: This paper introduces the design of a reconfigurable transmitarray operating within the D-band frequency range (110-170 GHz). The transmitarray unit cell is composed of three metal layers and two quartz dielectric substrates. It achieves a 1-bit phase ... ...

Abstract	This paper introduces the design of a reconfigurable transmitarray operating within the D-band frequency range (110-170 GHz). The transmitarray unit cell is composed of three metal layers and two quartz dielectric substrates. It achieves a 1-bit phase shift resolution through the alternating states of two innovative switches integrated into the active transmitting patch of the unit cell. To address the challenge of miniaturization in the D-band, compact switches compatible with the proposed unit cell dimensions are introduced. These switches are constructed using phase change materials (PCM) that change between amorphous and crystalline states when exposed to heat. The paper includes a full-wave simulation of the unit cell, demonstrating an insertion loss below 1.5 dB across a wide frequency band of 27%. Additionally, a 10 [Formula: see text] 10 elements transmitarray is synthesized using a numerical tool and its theoretical results are compared to full-wave electromagnetic simulations for validation purposes. The results indicate that by incorporating the proposed switches into the unit cell, the transmitarray achieves promising reconfiguration capabilities within the D-band. Moreover, the paper presents the architecture of a command line designed to bias the PCM switches. Notably, this command line represents a novel approach, as it enables individual biasing of each PCM switch using direct current (DC). The influence of these command lines on the transmitarray's performance is thoroughly investigated. Although there is a compromise in the 1-dB gain bandwidth, the overall behavior of the transmitarray remains encouraging.
Language	English
Publishing date	2024-02-05
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-024-53150-9
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Article ; Online: Dysfunctional mitochondria, disrupted levels of reactive oxygen species, and autophagy in B cells from common variable immunodeficiency patients.

Berman-Riu, Maria / Cunill, Vanesa / Clemente, Antonio / López-Gómez, Antonio / Pons, Jaime / Ferrer, Joana M

Frontiers in immunology

2024 Volume 15, Page(s) 1362995

Abstract: Introduction: Common Variable Immunodeficiency (CVID) patients are characterized by hypogammaglobulinemia and poor response to vaccination due to deficient generation of memory and antibody-secreting B cells. B lymphocytes are essential for the ... ...

Abstract	Introduction: Common Variable Immunodeficiency (CVID) patients are characterized by hypogammaglobulinemia and poor response to vaccination due to deficient generation of memory and antibody-secreting B cells. B lymphocytes are essential for the development of humoral immune responses, and mitochondrial function, hreactive oxygen species (ROS) production and autophagy are crucial for determining B-cell fate. However, the role of those basic cell functions in the differentiation of human B cells remains poorly investigated. Methods: We used flow cytometry to evaluate mitochondrial function, ROS production and autophagy processes in human naïve and memory B-cell subpopulations in unstimulated and stimulated PBMCs cultures. We aimed to determine whether any alterations in these processes could impact B-cell fate and contribute to the lack of B-cell differentiation observed in CVID patients. Results: We described that naïve CD19 Conclusion: The failure in ROS cell signalling could impair CVID naïve B cell activation and differentiation to memory B cells. Decreased levels of ROS in CVID memory CD19
MeSH term(s)	Humans ; Reactive Oxygen Species/metabolism ; Common Variable Immunodeficiency ; Tumor Necrosis Factor Receptor Superfamily, Member 7/metabolism ; B-Lymphocytes ; Antigens, CD19/metabolism ; Autophagy ; Mitochondria/metabolism
Chemical Substances	Reactive Oxygen Species ; Tumor Necrosis Factor Receptor Superfamily, Member 7 ; Antigens, CD19
Language	English
Publishing date	2024-03-26
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2024.1362995
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Article ; Online: OriPlex: Origami-enabled multiplexed detection of respiratory pathogens.

Vaquer, Andreu / Adrover-Jaume, Cristina / Clemente, Antonio / Viana, Julia / Rodríguez, Rocío / Rojo-Molinero, Estrella / Oliver, Antonio / de la Rica, Roberto

Biosensors & bioelectronics

2024 Volume 257, Page(s) 116341

Abstract: Origami biosensors leverage paper foldability to develop total analysis systems integrated in a single piece of paper. This capability can also be utilized to incorporate additional features that would be difficult to achieve with rigid substrates. In ... ...

Abstract	Origami biosensors leverage paper foldability to develop total analysis systems integrated in a single piece of paper. This capability can also be utilized to incorporate additional features that would be difficult to achieve with rigid substrates. In this article, we report a new design for 3D origami biosensors called OriPlex, which leverages the foldability of filter paper for the multiplexed detection of bacterial pathogens. OriPlex immunosensors detect pathogens by folding nanoparticle reservoirs containing different types of nanoprobes. This releases antibody-coated nanoparticles in a central channel where targets are captured through physical interactions. The OriPlex concept was demonstrated by detecting the respiratory pathogens Pseudomonas aeruginosa (PA) and Klebsiella pneumoniae (KP) with a limit of detection of 3.4·10
Language	English
Publishing date	2024-04-25
Publishing country	England
Document type	Journal Article
ZDB-ID	1011023-9
ISSN	1873-4235 ; 0956-5663
ISSN (online)	1873-4235
ISSN	0956-5663
DOI	10.1016/j.bios.2024.116341
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Article ; Online: Improving the Quantification of Colorimetric Signals in Paper-Based Immunosensors with an Open-Source Reader.

Russell, Steven M / Alba-Patiño, Alejandra / Vaquer, Andreu / Clemente, Antonio / de la Rica, Roberto

Sensors (Basel, Switzerland)

2022 Volume 22, Issue 5

Abstract: Measuring the colorimetric signals produced by the biospecific accumulation of colorimetric probes and recording the results is a key feature for next-generation paper-based rapid tests. Manual processing of these tests is time-consuming and prone to a ... ...

Abstract	Measuring the colorimetric signals produced by the biospecific accumulation of colorimetric probes and recording the results is a key feature for next-generation paper-based rapid tests. Manual processing of these tests is time-consuming and prone to a loss of accuracy when interpreting faint and patchy signals. Proprietary, closed-source readers and software companies offering automated smartphone-based assay readings have both been criticized for interoperability issues. Here, we introduce a minimal reader prototype composed of open-source hardware and open-source software that has the benefits of automatic assay quantification while avoiding the interoperability issues associated with closed-source readers. An image-processing algorithm was developed to automate the selection of an optimal region of interest and measure the average pixel intensity. When used to quantify signals produced by lateral flow immunoassays for detecting antibodies against SARS-CoV-2, results obtained with the proposed algorithm were comparable to those obtained with a manual method but with the advantage of improving the precision and accuracy when quantifying small spots or faint and patchy signals.
MeSH term(s)	Biosensing Techniques ; COVID-19/diagnosis ; Colorimetry/methods ; Humans ; Immunoassay/methods ; SARS-CoV-2
Language	English
Publishing date	2022-02-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2052857-7
ISSN	1424-8220 ; 1424-8220
ISSN (online)	1424-8220
ISSN	1424-8220
DOI	10.3390/s22051880
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Article ; Online: Spacer exchange in persistent periprosthetic joint infection: microbiological evaluation and survivorship analysis.

Clemente, Antonio / Cavagnaro, Luca / Russo, Antonio / Chiarlone, Francesco / Massè, Alessandro / Burastero, Giorgio

Archives of orthopaedic and trauma surgery

2022 Volume 143, Issue 3, Page(s) 1361–1370

Abstract: Purpose: The purposes of this study were to determine demographics and characteristics of patients who underwent spacer exchange for persistent infection in the setting of two-stage arthroplasty for periprosthetic joint infection, to describe the ... ...

Abstract	Purpose: The purposes of this study were to determine demographics and characteristics of patients who underwent spacer exchange for persistent infection in the setting of two-stage arthroplasty for periprosthetic joint infection, to describe the microbiology of pathogens involved, to analyze survivorship free from infection in these patients. Methods: The institutional prospectively collected database was reviewed to enroll patients with minimum 2 years follow-up. Patients who underwent two-stage procedure for septic arthritis were excluded, as were patients who had spacer fracture or dislocation. Results: A total of 34 patients (41 procedures) were included. Mean age was 65.0 ± 12.8 years. Mean follow-up was 53.4 ± 24.8 months. Mean number of previous procedures was 3.6 ± 1.2. A total of 27 (79.4%) patients underwent final reimplantation. The most frequently isolated pathogen in spacer exchange was Staphylococcus epidermidis (10 cases, 28.6%). Polymicrobial cultures were obtained from 9 (25.71%) patients, 10 (28.6%) presented culture-negative infections. A total of 11 (32.4%) resistant pathogens were isolated, and 16 (47.0%) difficult to treat pathogens were detected. Eradication rate was 78.8%. Overall survivorship of implants after final reimplantation was 72.8% at 51.8 months. Conclusion: Surgeons should be aware that subjects necessitating spacer exchange often present multiple comorbidities, previous staged revision failures, soft-tissue impairment and difficult to treat infection. In these patients, spacer exchange provides good clinical results and infection eradication, preventing arthrodesis or amputation.
MeSH term(s)	Humans ; Middle Aged ; Aged ; Arthroplasty, Replacement, Knee/methods ; Persistent Infection ; Prosthesis-Related Infections/surgery ; Arthroplasty, Replacement, Hip/methods ; Arthritis, Infectious/surgery ; Reoperation/methods ; Retrospective Studies ; Anti-Bacterial Agents/therapeutic use ; Treatment Outcome
Chemical Substances	Anti-Bacterial Agents
Language	English
Publishing date	2022-01-14
Publishing country	Germany
Document type	Journal Article
ZDB-ID	80407-1
ISSN	1434-3916 ; 0003-9330 ; 0344-8444
ISSN (online)	1434-3916
ISSN	0003-9330 ; 0344-8444
DOI	10.1007/s00402-021-04300-5
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Article: Rapid Identification and Classification of Pathogens That Produce Carbapenemases and Cephalosporinases with a Colorimetric Paper-Based Multisensor

Santopolo, Giulia / Clemente, Antonio / Rojo-Molinero, Estrella / Oliver, Antonio / Noé, Camilla / de la Rica, Roberto

Analytical chemistry. 2022 June 24, v. 94, no. 26

2022

Abstract: Infections caused by bacteria that produce β-lactamases (BLs) are a major problem in hospital settings. The phenotypic detection of these bacterial strains requires culturing samples prior to analysis. This procedure may take up to 72 h, and therefore it ...

Abstract	Infections caused by bacteria that produce β-lactamases (BLs) are a major problem in hospital settings. The phenotypic detection of these bacterial strains requires culturing samples prior to analysis. This procedure may take up to 72 h, and therefore it cannot be used to guide the administration of the first antibiotic regimen. Here, we propose a multisensor for identifying pathogens bearing different types of β-lactamases above the infectious dose threshold within 90 min that does not require culturing samples. Instead, bacterial cells are preconcentrated in the cellulose scaffold of a paper-based multisensor. Then, 12 assays are performed in parallel to identify whether the pathogens produce carbapenemases and/or cephalosporinases, including metallo-β-lactamases, extended-spectrum β-lactamases (ESBLs), and AmpC enzymes. The multisensor generates an array of colored spots that can be quantified with image processing software and whose interpretation leads to the detection of the different enzymes depending on their specificity toward the hydrolysis of certain antibiotics, and/or their pattern of inhibition or cofactor activation. The test was validated for the diagnosis of urinary tract infections. The inexpensive paper platform along with the uncomplicated colorimetric readout makes the proposed prototypes promising for developing fully automated platforms for streamlined clinical diagnosis.
Keywords	analytical chemistry ; antibiotics ; automation ; beta-lactamase ; cellulose ; colorimetry ; computer software ; hospitals ; hydrolysis ; paper ; phenotype ; urinary tract
Language	English
Dates of publication	2022-0624
Size	p. 9442-9449.
Publishing place	American Chemical Society
Document type	Article
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.2c01724
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Article ; Online: Clinical outcomes and survivorship of two-stage total hip or knee arthroplasty in septic arthritis: a retrospective analysis with a minimum five-year follow-up.

Russo, Antonio / Cavagnaro, Luca / Chiarlone, Francesco / Clemente, Antonio / Romagnoli, Sergio / Burastero, Giorgio

International orthopaedics

2021 Volume 45, Issue 7, Page(s) 1683–1691

Abstract: Purpose: Septic arthritis of the native joint is challenging for orthopedic surgeons because it may lead to wide bone defects and severe impairment of joint function. This study aimed to analyze clinical functional outcomes, the rate of infection ... ...

Abstract	Purpose: Septic arthritis of the native joint is challenging for orthopedic surgeons because it may lead to wide bone defects and severe impairment of joint function. This study aimed to analyze clinical functional outcomes, the rate of infection eradication, and survival of implants of patients who underwent two-stage arthroplasty for septic arthritis of the hip and knee. Methods: A retrospective single-centre analysis was conducted of patients treated for septic arthritis of the hip and knee joints through a two-stage surgery between 2012 and 2015. Clinical and radiological records were gathered from the prospectively collected Institutional Arthroplasty Registry. Patients' pre-operative Harris hip scores and Knee Society scores were compared with those obtained at the latest follow-up. Kaplan-Meier curves were generated to assess survival of implants. Results: Forty-seven patients were included. The mean follow-up was 85.2 ± 15.4 months. The Harris hip score improved from 39.4 ± 9.9 to 84.5 ± 10.8 points (p < 0.001). The Knee Society score improved from 40.7 ± 8.4 to 86.0 ± 7.8 points (p < 0.001). Knee Society score-function increased from 25.7 ± 14.2 to 85.4 ± 23.4 points (p < 0.001). The infection eradication rates were 92.0% and 90.9% in patients who underwent hip and knee operation, respectively (p = 0.891). Overall survivorship of implants after the second stage was 93.6%. Conclusions: Two-stage arthroplasty provides good to excellent clinical outcomes in cases of active septic arthritis of the hip and the knee, high rates of infection control, and implant survival.
MeSH term(s)	Arthritis, Infectious/epidemiology ; Arthritis, Infectious/surgery ; Arthroplasty, Replacement, Hip/adverse effects ; Arthroplasty, Replacement, Knee/adverse effects ; Follow-Up Studies ; Humans ; Reoperation ; Retrospective Studies ; Survivorship
Language	English
Publishing date	2021-03-27
Publishing country	Germany
Document type	Journal Article
ZDB-ID	80384-4
ISSN	1432-5195 ; 0341-2695
ISSN (online)	1432-5195
ISSN	0341-2695
DOI	10.1007/s00264-021-05013-5
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Article: Improved cytometric analysis of untouched lung leukocytes by enzymatic liquefaction of sputum samples.

Santopolo, Giulia / Clemente, Antonio / Rojo-Molinero, Estrella / Fernández, Sara / Álvarez, María Concepción / Oliver, Antonio / de la Rica, Roberto

Biological procedures online

2022 Volume 24, Issue 1, Page(s) 17

Abstract: Background: Phenotyping sputum-resident leukocytes and evaluating their functional status are essential analyses for exploring the cellular basis of pathological processes in the lungs, and flow cytometry is widely recognized as the gold-standard ... ...

Abstract	Background: Phenotyping sputum-resident leukocytes and evaluating their functional status are essential analyses for exploring the cellular basis of pathological processes in the lungs, and flow cytometry is widely recognized as the gold-standard technique to address them. However, sputum-resident leukocytes are found in respiratory samples which need to be liquefied prior to cytometric analysis. Traditional liquefying procedures involve the use of a reducing agent such as dithiothreitol (DTT) in temperature-controlled conditions, which does not homogenize respiratory samples efficiently and impairs cell viability and functionality. Methods: Here we propose an enzymatic method that rapidly liquefies samples by means of generating O Results: Enzymatically treated samples were better liquefied than those treated with DTT or PBS, which resulted in a more accurate cytometric analysis. Frequencies of all cell subsets analyzed within liquefied samples were comparable between liquefaction methods. However, the gentle cell handling rendered by the enzymatic method improves cell viability and retains in vivo functional characteristics of sputum-resident leukocytes (with regard to HLA-DR, CD63 and CD11b expression). Conclusion: In conclusion, the proposed enzymatic liquefaction method improves the cytometric analysis of respiratory samples and leaves the cells widely untouched for properly addressing functional analysis of lung leukocytes.
Language	English
Publishing date	2022-11-17
Publishing country	England
Document type	Journal Article
ZDB-ID	2027823-8
ISSN	1480-9222
ISSN	1480-9222
DOI	10.1186/s12575-022-00181-z
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