Article ; Online: Multiplexed orthogonal genome editing and transcriptional activation by Cas12a.
2018 Volume 16, Issue 1, Page(s) 51–54
Abstract: CRISPR-Cas9-based combinatorial perturbation approaches for orthogonal knockout and gene activation have been impeded by complex vector designs and co-delivery of multiple constructs. Here, we demonstrate that catalytically active CRISPR-Cas12a fused to ... ...
| Abstract | CRISPR-Cas9-based combinatorial perturbation approaches for orthogonal knockout and gene activation have been impeded by complex vector designs and co-delivery of multiple constructs. Here, we demonstrate that catalytically active CRISPR-Cas12a fused to a transcriptional-activator domain enables flexible switching between genome editing and transcriptional activation by altering guide length. By leveraging Cas12a-mediated CRISPR-RNA array processing, we illustrate that Cas12a-VPR enables simplified multiplexed knockout and transcriptional activation in vitro and in vivo. |
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| MeSH term(s) | Animals ; CRISPR-Cas Systems ; Cell Line, Tumor ; Gene Editing ; HEK293 Cells ; Humans ; Mice ; Transcriptional Activation | |||||
| Language | English | |||||
| Publishing date | 2018-12-17 | |||||
| Publishing country | United States | |||||
| Document type | Journal Article ; Research Support, Non-U.S. Gov't | |||||
| ZDB-ID | 2169522-2 | |||||
| ISSN | 1548-7105 ; 1548-7091 | |||||
| ISSN (online) | 1548-7105 | |||||
| ISSN | 1548-7091 | |||||
| DOI | 10.1038/s41592-018-0262-1 | |||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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