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  1. Article ; Online: Determination of aztreonam/ceftazidime-avibactam synergism and proposal of a new methodology for the evaluation of susceptibility in vitro.

    Collar, Gabriela da Silva / Moreira, Natália Kehl / Becker, Julia / Barth, Afonso Luís / Caierão, Juliana

    Diagnostic microbiology and infectious disease

    2024  Volume 109, Issue 2, Page(s) 116236

    Abstract: We proposed a new methodology, the microelution ATM/CZA (mATM/CZA), based on the antibiotic disc elution and the use of resazurin, for rapid (<4h) determination of in vitro susceptibility to aztreonam combined with ceftazidime-avibactam among ... ...

    Abstract We proposed a new methodology, the microelution ATM/CZA (mATM/CZA), based on the antibiotic disc elution and the use of resazurin, for rapid (<4h) determination of in vitro susceptibility to aztreonam combined with ceftazidime-avibactam among Enterobacterales. The mATM/CZA presented excellent accuracy with 1.9 %, 98.1 % and 100 % of major error, specificity and sensitivity, respectively. Furthermore, we assessed synergism between aztreonam and ceftazidime-avibactam in Enterobacterales and Pseudomonas aeruginosa, which was observed in 37/55 Enterobacterales and 31/56 P. aeruginosa. As reference methodologies (checkerboard, time-kill curve) are not compatible with the routine of the clinical microbiology laboratories, mATM/CZA is an important alternative to evaluate susceptibility of the combination in a scenario where its clinical use is increasingly important.
    MeSH term(s) Aztreonam/pharmacology ; Azabicyclo Compounds/pharmacology ; Drug Combinations ; Microbial Sensitivity Tests/methods ; Microbial Sensitivity Tests/standards ; Anti-Bacterial Agents/pharmacology ; Ceftazidime/pharmacology ; Humans ; Drug Synergism ; Pseudomonas aeruginosa/drug effects ; Enterobacteriaceae/drug effects ; Sensitivity and Specificity ; Xanthenes ; Oxazines
    Chemical Substances Aztreonam (G2B4VE5GH8) ; Azabicyclo Compounds ; Drug Combinations ; Anti-Bacterial Agents ; avibactam, ceftazidime drug combination ; Ceftazidime (9M416Z9QNR) ; resazurin (1FN9YD6968) ; Xanthenes ; Oxazines
    Language English
    Publishing date 2024-03-01
    Publishing country United States
    Document type Journal Article ; Evaluation Study
    ZDB-ID 604920-5
    ISSN 1879-0070 ; 0732-8893
    ISSN (online) 1879-0070
    ISSN 0732-8893
    DOI 10.1016/j.diagmicrobio.2024.116236
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1845: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Rapid colorimetric polymyxin B microelution directly from positive blood bottles: because patients with serious infections should not have to wait for results of culture-based methodologies.

    Collar, Gabriela da Silva / Becker, Julia / Moreira, Natália Kehl / Dornelles, Luana Silva / Mott, Mariana Preussler / Barth, Afonso Luís / Caierão, Juliana

    European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology

    2024  

    Abstract: Purpose: To evaluate the performance of the rapid colorimetric polymyxin B microelution (RCPEm) in determining polymyxin B resistance directly from Enterobacterales-positive blood cultures.: Methods: A set volume of positive blood culture bottles ( ... ...

    Abstract Purpose: To evaluate the performance of the rapid colorimetric polymyxin B microelution (RCPEm) in determining polymyxin B resistance directly from Enterobacterales-positive blood cultures.
    Methods: A set volume of positive blood culture bottles (diluted 1:10) was inoculated into a glucose-broth-phenol red solution (NP solution), where a polymyxin B disk was previously eluted (final concentration of 3 µg/mL). Test was read each 1 h for up to 4 h. Color change from red/orange to yellow indicated resistant isolates. Results were compared to the reference method, broth microdilution (BMD), performed from colonies grown on solid media from the same blood culture bottle.
    Results: One hundred fifty-two Enterobacterales-positive blood cultures were evaluated, 22.4% (34/152) of them resistant to polymyxin B (including 6.6% with borderline MICs). When performing directly from positive blood cultures (RCPEm-BC), specificity and sensitivity were 99.1% and 94.1%, respectively. Of note, 79.4% (27/34) of truly resistant isolates required 3 h of incubation, compared to the 18 ± 2 h incubation that microtiter plates of BMD demand before reading can be performed.
    Conclusions: RCPEm directly from blood cultures has great potential to be part of the routine of clinical microbiology laboratories to establish polymyxin B susceptibility, impacting outcome of patients with bloodstream infections caused by carbapenem-resistant Enterobacterales.
    Language English
    Publishing date 2024-05-11
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 603155-9
    ISSN 1435-4373 ; 0934-9723 ; 0722-2211
    ISSN (online) 1435-4373
    ISSN 0934-9723 ; 0722-2211
    DOI 10.1007/s10096-024-04846-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 1732: Show issues Location:
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  3. Article ; Online: Detection of KPC directly from positive blood cultures by MALDI-TOF: From research to the clinical microbiology laboratory routine.

    Moreira, Natália Kehl / Wilhelm, Camila Mörschbächer / Collar, Gabriela da Silva / Echevarria, Aymê Duarte / Becker, Júlia / Barth, Afonso Luís / Caierão, Juliana

    Journal of microbiological methods

    2024  Volume 221, Page(s) 106940

    Abstract: Bloodstream infections (BSI) caused by carbapenem-resistant Gram-negative bacilli (CR-GNB) are a subject of major clinical concern, mainly those associated with carbapenemase-producing isolates. Matrix-assisted laser desorption ionization time-of-flight ... ...

    Abstract Bloodstream infections (BSI) caused by carbapenem-resistant Gram-negative bacilli (CR-GNB) are a subject of major clinical concern, mainly those associated with carbapenemase-producing isolates. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been proposed to detect specific β-lactamases, including KPC. We aimed to detect KPC enzyme directly from positive blood cultures using MALDI-TOF MS. Overall, 146 clinical Gram-negative bacilli (46 CR-GNB) recovered from consecutive blood cultures were evaluated. Proteins were extracted using formic acid, isopropyl alcohol, and water and spotted onto a steel target plate using the double-layer sinapinic acid method. The relative ions intensity ≥120 arbitrary units (a.u.) of a peak close to 28,700 m/z indicated the presence of KPC. The results were compared to HRM-qPCR methodology. This specific peak was observed in 11/14 blood bottles with bla
    MeSH term(s) Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods ; Humans ; beta-Lactamases/genetics ; Blood Culture/methods ; Bacterial Proteins/genetics ; Sensitivity and Specificity ; Gram-Negative Bacteria/isolation & purification ; Gram-Negative Bacteria/enzymology ; Gram-Negative Bacteria/classification ; Gram-Negative Bacteria/genetics ; Bacteremia/microbiology ; Bacteremia/diagnosis ; Gram-Negative Bacterial Infections/microbiology ; Gram-Negative Bacterial Infections/diagnosis ; Gram-Negative Bacterial Infections/blood ; Anti-Bacterial Agents/pharmacology ; Carbapenems/pharmacology
    Chemical Substances carbapenemase
    Language English
    Publishing date 2024-05-01
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 604916-3
    ISSN 1872-8359 ; 0167-7012
    ISSN (online) 1872-8359
    ISSN 0167-7012
    DOI 10.1016/j.mimet.2024.106940
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1849: Show issues Location:
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  4. Article ; Online: Detection of polymyxins resistance among Enterobacterales: evaluation of available methods and proposal of a new rapid and feasible methodology.

    Collar, Gabriela da Silva / Moreira, Natália Kehl / Wink, Priscila Lamb / Barth, Afonso Luís / Raro, Otávio Hallal Ferreira / Dias, Cícero / de Lima Machado, Adriano / Mott, Mariana Preussler / Caierão, Juliana

    Annals of clinical microbiology and antimicrobials

    2023  Volume 22, Issue 1, Page(s) 71

    Abstract: Background: Fast and accurate detection of polymyxins resistance is necessary as they remain the last resources to treat infections caused by Carbapenem-resistant Enterobacterales in many regions. We evaluated the rapid colorimetric polymyxin B elution ( ...

    Abstract Background: Fast and accurate detection of polymyxins resistance is necessary as they remain the last resources to treat infections caused by Carbapenem-resistant Enterobacterales in many regions. We evaluated the rapid colorimetric polymyxin B elution (RCPE) and developed its miniaturized version, RCPE microelution (RCPEm), aiming to detect polymyxins resistance among Enterobacterales.
    Methods: The methodologies consist of exposing the bacterial population in a solution (NP solution) where polymyxin B disks were previously eluted to obtain a concentration of 2 µg/mL for RCPE and 3 µg/mL for RCPEm.
    Results: Two hundred sixty-seven Enterobacterales were evaluated, 90 (33.7%) resistant to polymyxin B by broth microdilution. It was observed 0.6% of major error (ME) by RCPE, with a specificity of 99.4%. The miniaturized version (RCPEm) presented the same ME and specificity values, but slightly higher sensitivity (97.8% vs. 95.6%) with 2.2% of very major error (VME).
    Conclusions: RCPE and RCPEm proved to be useful alternatives to determine polymyxin B susceptibility in clinical microbiology laboratories, presenting low cost, being easy to perform, and demanding short incubation time.
    MeSH term(s) Humans ; Polymyxins/pharmacology ; Polymyxin B/pharmacology ; Anti-Bacterial Agents/pharmacology ; Colistin ; Microbial Sensitivity Tests
    Chemical Substances Polymyxins ; Polymyxin B (J2VZ07J96K) ; Anti-Bacterial Agents ; Colistin (Z67X93HJG1)
    Language English
    Publishing date 2023-08-10
    Publishing country England
    Document type Journal Article
    ZDB-ID 2097873-X
    ISSN 1476-0711 ; 1476-0711
    ISSN (online) 1476-0711
    ISSN 1476-0711
    DOI 10.1186/s12941-023-00618-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Evaluation of EDTA and Dipicolinic Acid in Broth Microdilution with Polymyxin B as a Phenotypic Test to Detect the

    Wink, Priscila Lamb / Caierão, Juliana / Nunes, Aline Gabrielle Alves / Collar, Gabriela da Silva / Martins, Jessica Berghahn / Dalmolin, Tanise Vendruscolo / Pilonetto, Marcelo / Barth, Afonso Luís

    Microbial drug resistance (Larchmont, N.Y.)

    2019  Volume 26, Issue 4, Page(s) 329–333

    Abstract: Polymyxins (colistin and polymyxin B) have recently regained significant importance as last-line drugs to treat infectious diseases due to multidrug-resistant gram-negative bacteria. However, resistance to polymyxins has increased, and the recognition of ...

    Abstract Polymyxins (colistin and polymyxin B) have recently regained significant importance as last-line drugs to treat infectious diseases due to multidrug-resistant gram-negative bacteria. However, resistance to polymyxins has increased, and the recognition of plasmid-mediated resistance (by the
    MeSH term(s) Animals ; Anti-Bacterial Agents/pharmacology ; Colistin/pharmacology ; Drug Resistance, Multiple, Bacterial/genetics ; Edetic Acid/pharmacology ; Escherichia coli/drug effects ; Escherichia coli/genetics ; Escherichia coli Infections/drug therapy ; Escherichia coli Infections/microbiology ; Escherichia coli Proteins/genetics ; Humans ; Klebsiella pneumoniae/drug effects ; Klebsiella pneumoniae/genetics ; Microbial Sensitivity Tests/methods ; Picolinic Acids/pharmacology ; Polymyxin B/pharmacology ; Salmonella/drug effects ; Salmonella/genetics ; Sensitivity and Specificity
    Chemical Substances Anti-Bacterial Agents ; Escherichia coli Proteins ; MCR-1 protein, E coli ; Picolinic Acids ; Edetic Acid (9G34HU7RV0) ; Polymyxin B (J2VZ07J96K) ; dipicolinic acid (UE81S5CQ0G) ; Colistin (Z67X93HJG1)
    Language English
    Publishing date 2019-10-08
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1290490-9
    ISSN 1931-8448 ; 1076-6294
    ISSN (online) 1931-8448
    ISSN 1076-6294
    DOI 10.1089/mdr.2019.0172
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4435: Show issues Location:
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