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Article ; Online: Using continuous ST-segment monitoring.

Collins, Michelle L

2010 Volume 40 Suppl, Page(s) 11–13

MeSH term(s)	Electrocardiography/methods ; Humans ; Monitoring, Physiologic/methods ; Myocardial Ischemia/diagnosis ; Myocardial Ischemia/physiopathology
Language	English
Publishing date	2010
Publishing country	United States
Document type	Journal Article
ZDB-ID	197895-0
ISSN	1538-8689 ; 0360-4039
ISSN (online)	1538-8689
ISSN	0360-4039
DOI	10.1097/01.NURSE.0000368965.09753.04
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Using a Lean Six Sigma Approach to Yield Sustained Pressure Ulcer Prevention for Complex Critical Care Patients.

Donovan, Elizabeth A / Manta, Christine J / Goldsack, Jennifer C / Collins, Michelle L

The Journal of nursing administration

2016 Volume 46, Issue 1, Page(s) 43–48

Abstract: Background: Under value-based purchasing, Medicare withholds reimbursements for hospital-acquired pressure ulcer occurrence and rewards hospitals that meet performance standards. With little evidence of a validated prevention process, nurse managers are ...

Abstract	Background: Under value-based purchasing, Medicare withholds reimbursements for hospital-acquired pressure ulcer occurrence and rewards hospitals that meet performance standards. With little evidence of a validated prevention process, nurse managers are challenged to find evidence-based interventions. Objective: The aim of this study was to reduce the unit-acquired pressure ulcer (UAPU) rate on targeted intensive care and step-down units by 15% using Lean Six Sigma (LSS) methodology. Methods: An interdisciplinary team designed a pilot program using LSS methodology to test 4 interventions: standardized documentation, equipment monitoring, patient out-of-bed-to-chair monitoring, and a rounding checklist. Results: During the pilot, the UAPU rate decreased from 4.4% to 2.8%, exceeding the goal of a 15% reduction. The rate remained below the goal through the program control phase at 2.9%, demonstrating a statistically significant reduction after intervention implementation. Conclusions: The program significantly reduced UAPU rates in high-risk populations. LSS methodologies are a sustainable approach to reducing hospital-acquired conditions that should be broadly tested and implemented.
MeSH term(s)	Critical Care/methods ; Delaware ; Evidence-Based Nursing/methods ; Humans ; Iatrogenic Disease/prevention & control ; Nursing Evaluation Research ; Pilot Projects ; Pressure Ulcer/prevention & control ; Skin Care/methods
Language	English
Publishing date	2016-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	193141-6
ISSN	1539-0721 ; 1539-073X ; 0002-0443
ISSN (online)	1539-0721 ; 1539-073X
ISSN	0002-0443
DOI	10.1097/NNA.0000000000000291
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Exploration of Social, Environmental, and Familial Influences on the Sexual Health Practices of Urban African American Adolescents.

Collins, Michelle L / Baiardi, Janet M / Tate, Nutrena H / Rouen, Patricia A

Western journal of nursing research

2015 Volume 37, Issue 11, Page(s) 1441–1457

Abstract: Despite efforts to examine social influences and provide interventions that lead to safer sexual practices for African American (AA) adolescent girls, statistics have demonstrated this population continues to have the highest rates of teen pregnancy and ... ...

Abstract	Despite efforts to examine social influences and provide interventions that lead to safer sexual practices for African American (AA) adolescent girls, statistics have demonstrated this population continues to have the highest rates of teen pregnancy and sexually transmitted infections (STIs) including HIV when compared with adolescent girls of other races. Guided by the principles of Black Feminist Thought and Photovoice and focus group methodology, this descriptive study aimed to discover the social realities influencing a group of eight AA adolescent girls aged 15 to 19 years. Data from individual interviews and a focus group session were collected from November 11, 2012, to February 1, 2013. Analysis of selected photographs, interviews, and focus group conversations through a feminist lens suggests AA adolescents of low socioeconomic status continue to foster racial and mental inferiority stereotypes by using their sexuality as a bargaining tool for acceptance, value, and even love.
MeSH term(s)	Adolescent ; Adolescent Behavior/psychology ; African Americans/psychology ; Family/ethnology ; Family/psychology ; Female ; Health Knowledge, Attitudes, Practice ; Humans ; Pregnancy ; Risk Factors ; Sexual Behavior/psychology ; Sexually Transmitted Diseases/prevention & control ; Sexually Transmitted Diseases/psychology ; United States/ethnology ; Urban Population ; Young Adult
Language	English
Publishing date	2015-11
Publishing country	United States
Document type	Journal Article
ZDB-ID	632788-6
ISSN	1552-8456 ; 0193-9459
ISSN (online)	1552-8456
ISSN	0193-9459
DOI	10.1177/0193945914539794
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Article ; Online: Improving Nursing Knowledge of Alcohol Withdrawal: Second Generation Education Strategies.

Berl, Kimberly / Collins, Michelle L / Melson, Jo / Mooney, Ruth / Muffley, Cheryl / Wright-Glover, Angela

Journal for nurses in professional development

2015 Volume 31, Issue 6, Page(s) 328–332

Abstract: Christiana Care Health System implemented a Care Management Guideline for Alcohol Withdrawal Symptom Management, which provided direction for inpatient screening for alcohol withdrawal risk, assessment, and treatment. Nurses educated on its use expressed ...

Abstract	Christiana Care Health System implemented a Care Management Guideline for Alcohol Withdrawal Symptom Management, which provided direction for inpatient screening for alcohol withdrawal risk, assessment, and treatment. Nurses educated on its use expressed confusion with the use of the assessment tools, pharmacokinetics, and pathophysiology of alcohol withdrawal and delirium tremens. Reeducation was provided by nursing professional development specialists. Pre- and postsurveys revealed that nurses were more confident in caring for patients with alcohol withdrawal.
MeSH term(s)	Alcohol Withdrawal Delirium/therapy ; Alcoholism/diagnosis ; Clinical Competence ; Clinical Protocols/standards ; Educational Measurement ; Focus Groups ; Humans ; Mass Screening ; Nursing Staff, Hospital/education ; Risk Assessment ; Staff Development/methods
Language	English
Publishing date	2015-03-01
Publishing country	United States
Document type	Journal Article
ISSN	2169-981X
ISSN (online)	2169-981X
DOI	10.1097/NND.0000000000000176
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Article: Creation of a stepdown nurse internship program.

Collins, Michelle L / Thomas, Tamekia L

Journal for nurses in staff development : JNSD : official journal of the National Nursing Staff Development Organization

2005 Volume 21, Issue 3, Page(s) 115–119

Abstract: Within Christiana Care Health System, an opportunity evolved to develop a nurse recruitment strategy for our stepdown units. Being a large teaching system, there was specific need for nursing education at this entry level. Through collaboration of ... ...

Abstract	Within Christiana Care Health System, an opportunity evolved to develop a nurse recruitment strategy for our stepdown units. Being a large teaching system, there was specific need for nursing education at this entry level. Through collaboration of nursing colleagues, the Stepdown Nurse Internship Program was created. The program encompassed clinical orientation and didactic classes. Of notable significance at the completion of the program were the Basic Knowledge Assessment Test score improvement and the nurse retention rate.
MeSH term(s)	Curriculum ; Delaware ; Education, Nursing, Continuing/methods ; Education, Nursing, Continuing/organization & administration ; Humans ; Inservice Training/methods ; Inservice Training/organization & administration ; Internship, Nonmedical/methods ; Internship, Nonmedical/organization & administration ; Nursing Evaluation Research/methods ; Personnel Selection/methods ; Preceptorship/organization & administration ; Program Development/methods
Language	English
Publishing date	2005-05-25
Publishing country	United States
Document type	Journal Article
ZDB-ID	632730-8
ISSN	1098-7886 ; 0882-0627
ISSN	1098-7886 ; 0882-0627
DOI	10.1097/00124645-200505000-00007
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Using the gene pulser MXcell electroporation system to transfect primary cells with high efficiency.

McCoy, Adam M / Collins, Michelle L / Ugozzoli, Luis A

Journal of visualized experiments : JoVE

2010 , Issue 35

Abstract: It is becoming increasingly apparent that electroporation is the most effective way to introduce plasmid DNA or siRNA into primary cells. The Gene Pulser MXcell electroporation system and Gene Pulser electroporation buffer (Bio-Rad) were specifically ... ...

Abstract	It is becoming increasingly apparent that electroporation is the most effective way to introduce plasmid DNA or siRNA into primary cells. The Gene Pulser MXcell electroporation system and Gene Pulser electroporation buffer (Bio-Rad) were specifically developed to easily transfect nucleic acids into mammalian cells and difficult-to-transfect cells, such as primary and stem cells. We will demonstrate how to perform a simple experiment to quickly identify the best electroporation conditions. We will demonstrate how to run several samples through a range of electroporation conditions so that an experiment can be conducted at the same time as optimization is performed. We will also show how optimal conditions identified using 96-well electroporation plates can be used with standard electroporation cuvettes, facilitating the switch from electroporation plates to electroporation cuvettes while maintaining the same electroporation efficiency. In the video, we will also discuss some of the key factors that can lead to the success or failure of electroporation experiments.
MeSH term(s)	Animals ; Electroporation/instrumentation ; Electroporation/methods ; Green Fluorescent Proteins/analysis ; Green Fluorescent Proteins/biosynthesis ; Green Fluorescent Proteins/genetics ; Humans ; Microscopy, Fluorescence ; Plasmids/administration & dosage ; Plasmids/genetics ; RNA, Small Interfering/administration & dosage ; RNA, Small Interfering/genetics ; Transfection/instrumentation ; Transfection/methods
Chemical Substances	RNA, Small Interfering ; Green Fluorescent Proteins (147336-22-9)
Language	English
Publishing date	2010-01-07
Publishing country	United States
Document type	Journal Article ; Video-Audio Media
ZDB-ID	2259946-0
ISSN	1940-087X ; 1940-087X
ISSN (online)	1940-087X
ISSN	1940-087X
DOI	10.3791/1662
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Two major accretion epochs in M31 from two distinct populations of globular clusters.

Mackey, Dougal / Lewis, Geraint F / Brewer, Brendon J / Ferguson, Annette M N / Veljanoski, Jovan / Huxor, Avon P / Collins, Michelle L M / Côté, Patrick / Ibata, Rodrigo A / Irwin, Mike J / Martin, Nicolas / McConnachie, Alan W / Peñarrubia, Jorge / Tanvir, Nial / Wan, Zhen

Nature

2019 Volume 574, Issue 7776, Page(s) 69–71

Abstract: Large galaxies grow through the accumulation of dwarf ... ...

Abstract	Large galaxies grow through the accumulation of dwarf galaxies
Language	English
Publishing date	2019-10-02
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	120714-3
ISSN	1476-4687 ; 0028-0836
ISSN (online)	1476-4687
ISSN	0028-0836
DOI	10.1038/s41586-019-1597-1
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Article: Using the gene pulser mxcell electroporation system to transfect primary cells with high efficiency

McCoy, Adam M / Collins, Michelle L / Ugozzoli, Luis A

Journal of visualized experiments. 2010 Jan. 07, , no. 35

2010

Abstract	It is becoming increasingly apparent that electroporation is the most effective way to introduce plasmid DNA or siRNA into primary cells. The Gene Pulser MXcell electroporation system and Gene Pulser electroporation buffer (Bio-Rad) were specifically developed to easily transfect nucleic acids into mammalian cells and difficult-to-transfect cells, such as primary and stem cells. We will demonstrate how to perform a simple experiment to quickly identify the best electroporation conditions. We will demonstrate how to run several samples through a range of electroporation conditions so that an experiment can be conducted at the same time as optimization is performed. We will also show how optimal conditions identified using 96-well electroporation plates can be used with standard electroporation cuvettes, facilitating the switch from electroporation plates to electroporation cuvettes while maintaining the same electroporation efficiency. In the video, we will also discuss some of the key factors that can lead to the success or failure of electroporation experiments.
Keywords	electroporation ; genes ; mammals ; plasmids ; small interfering RNA ; stem cells
Language	English
Dates of publication	2010-0107
Size	p. e1662.
Publishing place	Journal of Visualized Experiments
Document type	Article
ZDB-ID	2259946-0
ISSN	1940-087X
ISSN	1940-087X
DOI	10.3791/1662
Database	NAL-Catalogue (AGRICOLA)

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Article: Using an automated cell counter to simplify gene expression studies: sirna knockdown of il-4 dependent gene expression in namalwa cells

McCoy, Adam M / Litterst, Claudia / Collins, Michelle L / Ugozzoli, Luis A

Journal of visualized experiments. 2010 Apr. 14, , no. 38

2010

Abstract: The use of siRNA mediated gene knockdown is continuing to be an important tool in studies of gene expression. siRNA studies are being conducted not only to study the effects of downregulating single genes, but also to interrogate signaling pathways and ... ...

Abstract	The use of siRNA mediated gene knockdown is continuing to be an important tool in studies of gene expression. siRNA studies are being conducted not only to study the effects of downregulating single genes, but also to interrogate signaling pathways and other complex interaction networks. These pathway analyses require both the use of relevant cellular models and methods that cause less perturbation to the cellular physiology. Electroporation is increasingly being used as an effective way to introduce siRNA and other nucleic acids into difficult to transfect cell lines and primary cells without altering the signaling pathway under investigation. There are multiple critical steps to a successful siRNA experiment, and there are ways to simplify the work while improving the data quality at several experimental stages. To help you get started with your siRNA mediated gene knockdown project, we will demonstrate how to perform a pathway study complete from collecting and counting the cells prior to electroporation through post transfection real-time PCR gene expression analysis. The following study investigates the role of the transcriptional activator STAT6 in IL-4 dependent gene expression of CCL17 in a Burkitt lymphoma cell line (Namalwa). The techniques demonstrated are useful for a wide range of siRNA-based experiments on both adherent and suspension cells. We will also show how to streamline cell counting with the TC10 automated cell counter, how to electroporate multiple samples simultaneously using the MXcell electroporation system, and how to simultaneously assess RNA quality and quantity with the Experion automated electrophoresis system.
Keywords	automation ; cell lines ; chemokine CCL17 ; data quality ; electrophoresis ; electroporation ; gene expression ; gene expression regulation ; gene targeting ; genes ; interleukin-4 ; models ; neoplasm cells ; physiology ; quantitative polymerase chain reaction ; signal transduction ; small interfering RNA ; transactivators ; transfection
Language	English
Dates of publication	2010-0414
Size	p. e1904.
Publishing place	Journal of Visualized Experiments
Document type	Article
ZDB-ID	2259946-0
ISSN	1940-087X
ISSN	1940-087X
DOI	10.3791/1904
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Using an automated cell counter to simplify gene expression studies: siRNA knockdown of IL-4 dependent gene expression in Namalwa cells.

McCoy, Adam M / Litterst, Claudia / Collins, Michelle L / Ugozzoli, Luis A

Journal of visualized experiments : JoVE

2010 , Issue 38

Abstract	The use of siRNA mediated gene knockdown is continuing to be an important tool in studies of gene expression. siRNA studies are being conducted not only to study the effects of downregulating single genes, but also to interrogate signaling pathways and other complex interaction networks. These pathway analyses require both the use of relevant cellular models and methods that cause less perturbation to the cellular physiology. Electroporation is increasingly being used as an effective way to introduce siRNA and other nucleic acids into difficult to transfect cell lines and primary cells without altering the signaling pathway under investigation. There are multiple critical steps to a successful siRNA experiment, and there are ways to simplify the work while improving the data quality at several experimental stages. To help you get started with your siRNA mediated gene knockdown project, we will demonstrate how to perform a pathway study complete from collecting and counting the cells prior to electroporation through post transfection real-time PCR gene expression analysis. The following study investigates the role of the transcriptional activator STAT6 in IL-4 dependant gene expression of CCL17 in a Burkitt lymphoma cell line (Namalwa). The techniques demonstrated are useful for a wide range of siRNA-based experiments on both adherent and suspension cells. We will also show how to streamline cell counting with the TC10 automated cell counter, how to electroporate multiple samples simultaneously using the MXcell electroporation system, and how to simultaneously assess RNA quality and quantity with the Experion automated electrophoresis system.
MeSH term(s)	Burkitt Lymphoma/genetics ; Burkitt Lymphoma/metabolism ; Burkitt Lymphoma/pathology ; Cell Count/instrumentation ; Cell Line, Tumor ; Electroporation/instrumentation ; Electroporation/methods ; Gene Knockdown Techniques/instrumentation ; Gene Knockdown Techniques/methods ; Humans ; Interleukin-4/biosynthesis ; Interleukin-4/genetics ; RNA, Small Interfering/administration & dosage ; RNA, Small Interfering/genetics
Chemical Substances	RNA, Small Interfering ; Interleukin-4 (207137-56-2)
Language	English
Publishing date	2010-04-14
Publishing country	United States
Document type	Journal Article ; Video-Audio Media
ZDB-ID	2259946-0
ISSN	1940-087X ; 1940-087X
ISSN (online)	1940-087X
ISSN	1940-087X
DOI	10.3791/1904
Database	MEDical Literature Analysis and Retrieval System OnLINE

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