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  1. Article ; Online: Structural analysis of cancer-relevant TCR-CD3 and peptide-MHC complexes by cryoEM.

    Saotome, Kei / Dudgeon, Drew / Colotti, Kiersten / Moore, Michael J / Jones, Jennifer / Zhou, Yi / Rafique, Ashique / Yancopoulos, George D / Murphy, Andrew J / Lin, John C / Olson, William C / Franklin, Matthew C

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 2401

    Abstract: The recognition of antigenic peptide-MHC (pMHC) molecules by T-cell receptors (TCR) initiates the T-cell mediated immune response. Structural characterization is key for understanding the specificity of TCR-pMHC interactions and informing the development ...

    Abstract The recognition of antigenic peptide-MHC (pMHC) molecules by T-cell receptors (TCR) initiates the T-cell mediated immune response. Structural characterization is key for understanding the specificity of TCR-pMHC interactions and informing the development of therapeutics. Despite the rapid rise of single particle cryoelectron microscopy (cryoEM), x-ray crystallography has remained the preferred method for structure determination of TCR-pMHC complexes. Here, we report cryoEM structures of two distinct full-length α/β TCR-CD3 complexes bound to their pMHC ligand, the cancer-testis antigen HLA-A2/MAGEA4 (230-239). We also determined cryoEM structures of pMHCs containing MAGEA4 (230-239) peptide and the closely related MAGEA8 (232-241) peptide in the absence of TCR, which provided a structural explanation for the MAGEA4 preference displayed by the TCRs. These findings provide insights into the TCR recognition of a clinically relevant cancer antigen and demonstrate the utility of cryoEM for high-resolution structural analysis of TCR-pMHC interactions.
    MeSH term(s) Humans ; Cryoelectron Microscopy ; Protein Binding ; Receptors, Antigen, T-Cell/metabolism ; Peptides/chemistry ; Histocompatibility Antigens/metabolism ; Neoplasms ; Major Histocompatibility Complex
    Chemical Substances Receptors, Antigen, T-Cell ; Peptides ; Histocompatibility Antigens
    Language English
    Publishing date 2023-04-26
    Publishing country England
    Document type Journal Article
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-37532-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Structural Analysis of Cancer-relevant TCR-CD3 and Peptide-MHC Complexes by CryoEM.

    Saotome, Kei / Dudgeon, Drew / Colotti, Kiersten / Moore, Michael J / Jones, Jennifer / Zhou, Yi / Rafique, Ashique / Yancopoulos, George D / Murphy, Andrew J / Lin, John C / Olson, William C / Franklin, Matthew C

    Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada

    2023  Volume 29, Issue 29 Suppl 1, Page(s) 892

    Language English
    Publishing date 2023-08-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 1385710-1
    ISSN 1435-8115 ; 1431-9276
    ISSN (online) 1435-8115
    ISSN 1431-9276
    DOI 10.1093/micmic/ozad067.440
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Genome and transcriptome of a pathogenic yeast, Candida nivariensis.

    Fan, Yunfan / Gale, Andrew N / Bailey, Anna / Barnes, Kali / Colotti, Kiersten / Mass, Michal / Morina, Luke B / Robertson, Bailey / Schwab, Remy / Tselepidakis, Niki / Timp, Winston

    G3 (Bethesda, Md.)

    2021  Volume 11, Issue 7

    Abstract: We present a highly contiguous genome and transcriptome of the pathogenic yeast, Candida nivariensis. We sequenced both the DNA and RNA of this species using both the Oxford Nanopore Technologies and Illumina platforms. We assembled the genome into an 11. ...

    Abstract We present a highly contiguous genome and transcriptome of the pathogenic yeast, Candida nivariensis. We sequenced both the DNA and RNA of this species using both the Oxford Nanopore Technologies and Illumina platforms. We assembled the genome into an 11.8 Mb draft composed of 16 contigs with an N50 of 886 Kb, including a circular mitochondrial sequence of 28 Kb. Using direct RNA nanopore sequencing and Illumina cDNA sequencing, we constructed an annotation of our new assembly, supplemented by lifting over genes from Saccharomyces cerevisiae and Candida glabrata.
    MeSH term(s) Saccharomyces cerevisiae ; Transcriptome ; Genome ; RNA ; Sequence Analysis, DNA
    Chemical Substances RNA (63231-63-0)
    Language English
    Publishing date 2021-04-22
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ZDB-ID 2629978-1
    ISSN 2160-1836 ; 2160-1836
    ISSN (online) 2160-1836
    ISSN 2160-1836
    DOI 10.1093/g3journal/jkab137
    Database MEDical Literature Analysis and Retrieval System OnLINE

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