Article ; Online: Antibody Light Chains: Key to Increased Monoclonal Antibody Yields in Expi293 Cells?
Antibodies (Basel, Switzerland)
2022 Volume 11, Issue 2
Abstract: When constructing isogenic recombinant IgM-IgG pairs, we discovered that μ heavy chains strongly prefer partnering with λ light chains for optimal IgM expression in transiently cotransfected Expi293 cells. When μ chains were paired with κ light chains, ... ...
| Abstract | When constructing isogenic recombinant IgM-IgG pairs, we discovered that μ heavy chains strongly prefer partnering with λ light chains for optimal IgM expression in transiently cotransfected Expi293 cells. When μ chains were paired with κ light chains, IgM yields were low but increased by logs-up to 20,000 X-by using λ chains instead. Switching light chains did not alter epitope specificity. For dimeric IgA2, optimal expression involved pairing with λ chains, whereas light-chain preference varied for other immunoglobulin classes. In summary, recombinant IgM production can be drastically increased by using λ chains, an important finding in the use of IgM for mucosal immunoprophylaxis. |
|---|---|
| Language | English |
| Publishing date | 2022-05-18 |
| Publishing country | Switzerland |
| Document type | Journal Article |
| ZDB-ID | 2661514-9 |
| ISSN | 2073-4468 ; 2073-4468 |
| ISSN (online) | 2073-4468 |
| ISSN | 2073-4468 |
| DOI | 10.3390/antib11020037 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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