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  1. Article ; Online: Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry.

    Perrenoud, Loni / Conley, James / Berg, Leslie J

    Journal of visualized experiments : JoVE

    2022  , Issue 190

    Abstract: Calcium influx in response to T-cell receptor stimulation is a common measure of T-cell signaling. Several calcium indicator dyes have been developed to assess calcium signaling by band-pass flow cytometry. This protocol is designed to measure calcium ... ...

    Abstract Calcium influx in response to T-cell receptor stimulation is a common measure of T-cell signaling. Several calcium indicator dyes have been developed to assess calcium signaling by band-pass flow cytometry. This protocol is designed to measure calcium responses in primary murine T-cells using full spectrum flow cytometry. Total splenocytes are labeled with the ratiometric calcium indicator dye Indo-1, along with a panel of fluorochrome-conjugated antibodies to cell surface molecules. Leveraging the capabilities of full spectrum flow cytometry provides a platform for utilizing a wide array of cell surface stains in combination with Indo-1. Cells are then analyzed in real-time at 37 °C before and after the addition of an anti-CD3 antibody to stimulate the T-cell receptor. After unmixing the spectral signals, the ratio of calcium-bound to calcium-free Indo-1 is calculated and can be visualized over time for each gated population of splenocytes. This technique can allow for the simultaneous analysis of calcium responses in multiple cell populations.
    MeSH term(s) Animals ; Mice ; T-Lymphocytes ; Flow Cytometry/methods ; Calcium/metabolism ; Receptors, Antigen, T-Cell/metabolism ; Cytoplasm/metabolism
    Chemical Substances Calcium (SY7Q814VUP) ; Receptors, Antigen, T-Cell
    Language English
    Publishing date 2022-12-16
    Publishing country United States
    Document type Journal Article ; Video-Audio Media ; Research Support, N.I.H., Extramural
    ZDB-ID 2259946-0
    ISSN 1940-087X ; 1940-087X
    ISSN (online) 1940-087X
    ISSN 1940-087X
    DOI 10.3791/64526
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Light chain crystalline podocytopathy.

    Nasr, Samih H / Conley, James / Said, Samar M

    Kidney international

    2021  Volume 100, Issue 3, Page(s) 713

    MeSH term(s) Heterocyclic Compounds ; Humans ; Kidney Diseases ; Podocytes
    Chemical Substances Heterocyclic Compounds
    Language English
    Publishing date 2021-08-22
    Publishing country United States
    Document type Case Reports
    ZDB-ID 120573-0
    ISSN 1523-1755 ; 0085-2538
    ISSN (online) 1523-1755
    ISSN 0085-2538
    DOI 10.1016/j.kint.2021.02.031
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 797: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Medical magnificat: Souls proclaiming God's greatness.

    Conley, James D

    The Linacre quarterly

    2015  Volume 82, Issue 3, Page(s) 203–206

    Language English
    Publishing date 2015-08
    Publishing country United States
    Document type Journal Article
    ZDB-ID 604903-5
    ISSN 2050-8549 ; 0024-3639
    ISSN (online) 2050-8549
    ISSN 0024-3639
    DOI 10.1179/0024363915Z.000000000119
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 2617: Show issues Location:
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  4. Article ; Online: TCR signaling: it's all about the numbers.

    Conley, James M / Berg, Leslie J

    Nature immunology

    2019  Volume 20, Issue 11, Page(s) 1415–1416

    MeSH term(s) Animals ; Humans ; Lymphocyte Activation/immunology ; Mass Spectrometry/methods ; Protein Interaction Domains and Motifs/immunology ; Receptors, Antigen, T-Cell/immunology ; Signal Transduction/immunology ; T-Lymphocytes/immunology
    Chemical Substances Receptors, Antigen, T-Cell
    Language English
    Publishing date 2019-10-07
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2016987-5
    ISSN 1529-2916 ; 1529-2908
    ISSN (online) 1529-2916
    ISSN 1529-2908
    DOI 10.1038/s41590-019-0520-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5294: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 42: Show issues

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  5. Article ; Online: Activation of the Tec Kinase ITK Controls Graded IRF4 Expression in Response to Variations in TCR Signal Strength.

    Conley, James M / Gallagher, Michael P / Rao, Anjana / Berg, Leslie J

    Journal of immunology (Baltimore, Md. : 1950)

    2020  Volume 205, Issue 2, Page(s) 335–345

    Abstract: TCR signal strength is critical for ... ...

    Abstract TCR signal strength is critical for CD8
    MeSH term(s) Animals ; CD8-Positive T-Lymphocytes/immunology ; Cell Proliferation ; Cells, Cultured ; Female ; Gene Expression Regulation ; Interferon Regulatory Factors/genetics ; Interferon Regulatory Factors/metabolism ; Lymphocyte Activation ; Male ; Mice ; Mice, Knockout ; Protein-Tyrosine Kinases/genetics ; Protein-Tyrosine Kinases/metabolism ; Receptors, Antigen, T-Cell/metabolism ; Signal Transduction
    Chemical Substances Interferon Regulatory Factors ; Receptors, Antigen, T-Cell ; interferon regulatory factor-4 ; Tec protein-tyrosine kinase (EC 2.7.1.-) ; Protein-Tyrosine Kinases (EC 2.7.10.1) ; emt protein-tyrosine kinase (EC 2.7.10.2)
    Language English
    Publishing date 2020-06-03
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 3056-9
    ISSN 1550-6606 ; 0022-1767 ; 1048-3233 ; 1047-7381
    ISSN (online) 1550-6606
    ISSN 0022-1767 ; 1048-3233 ; 1047-7381
    DOI 10.4049/jimmunol.1900853
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.37: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 28: Show issues

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  6. Article ; Online: Hierarchy of signaling thresholds downstream of the T cell receptor and the Tec kinase ITK.

    Gallagher, Michael P / Conley, James M / Vangala, Pranitha / Garber, Manuel / Reboldi, Andrea / Berg, Leslie J

    Proceedings of the National Academy of Sciences of the United States of America

    2021  Volume 118, Issue 35

    Abstract: The strength of peptide:MHC interactions with the T cell receptor (TCR) is correlated with the time to first cell division, the relative scale of the effector cell response, and the graded expression of activation-associated proteins like IRF4. To ... ...

    Abstract The strength of peptide:MHC interactions with the T cell receptor (TCR) is correlated with the time to first cell division, the relative scale of the effector cell response, and the graded expression of activation-associated proteins like IRF4. To regulate T cell activation programming, the TCR and the TCR proximal interleukin-2-inducible T cell kinase (ITK) simultaneously trigger many biochemically separate signaling cascades. T cells lacking ITK exhibit selective impairments in effector T cell responses after activation, but under the strongest signaling conditions, ITK activity is dispensable. To gain insight into whether TCR signal strength and ITK activity tune observed graded gene expression through the unequal activation of distinct signaling pathways, we examined Erk1/2 phosphorylation or nuclear factor of activated T cells (NFAT) and nuclear factor (NF)-κB translocation in naïve OT-I CD8
    MeSH term(s) Animals ; Cells, Cultured ; DNA/metabolism ; Female ; Gene Expression Regulation ; Lymphocyte Activation ; MAP Kinase Signaling System/genetics ; Male ; Mice ; NF-kappa B/metabolism ; NFATC Transcription Factors/metabolism ; Protein-Tyrosine Kinases/metabolism ; Receptors, Antigen, T-Cell/metabolism ; Signal Transduction/genetics ; T-Lymphocytes/enzymology ; T-Lymphocytes/immunology ; T-Lymphocytes/metabolism
    Chemical Substances NF-kappa B ; NFATC Transcription Factors ; Nfatc2 protein, mouse ; Receptors, Antigen, T-Cell ; DNA (9007-49-2) ; Tec protein-tyrosine kinase (EC 2.7.1.-) ; Protein-Tyrosine Kinases (EC 2.7.10.1) ; emt protein-tyrosine kinase (EC 2.7.10.2)
    Language English
    Publishing date 2021-08-24
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.2025825118
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 1148: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  7. Article: Peptide Antigen Concentration Modulates Digital NFAT1 Activation in Primary Mouse Naive CD8

    Gallagher, Michael P / Conley, James M / Berg, Leslie J

    ImmunoHorizons

    2018  Volume 2, Issue 7, Page(s) 208–215

    Abstract: Circulating naive T cells exist in a quiescent state. After TCR contact with the cognate peptide presented by APCs in secondary lymphoid structures, T cells undergo a period of rapid transcriptional changes that set the stage for fate-determining ... ...

    Abstract Circulating naive T cells exist in a quiescent state. After TCR contact with the cognate peptide presented by APCs in secondary lymphoid structures, T cells undergo a period of rapid transcriptional changes that set the stage for fate-determining effector or memory programming. We describe a novel method to analyze TCR signaling pathway activation in nuclei isolated from primary mouse naive T cells after stimulation with natural peptide Ags. We prelabeled cells with cell tracking dye to easily distinguish CD8
    Language English
    Publishing date 2018-07-02
    Publishing country United States
    Document type Journal Article
    ISSN 2573-7732
    ISSN 2573-7732
    DOI 10.4049/immunohorizons.1800032
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Working-class formation in Canada

    Conley, James

    Social inequality in Canada : patterns, problems, and policies , p. 44-60

    2009  , Page(s) 44–60

    Author's details James Conley
    Keywords Soziale Ungleichheit ; Wirtschaftliche Macht ; Arbeiterklasse ; Kanada
    Language English
    Publisher Pearson Prentice Hall
    Publishing place Toronto
    Document type Article
    ISBN 978-013-198-475-2 ; 013-198-475-6
    Database ECONomics Information System

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  9. Article: T Cells and Gene Regulation: The Switching On and Turning Up of Genes after T Cell Receptor Stimulation in CD8 T Cells.

    Conley, James M / Gallagher, Michael P / Berg, Leslie J

    Frontiers in immunology

    2016  Volume 7, Page(s) 76

    Abstract: Signaling downstream of the T cell receptor (TCR) is directly regulated by the dose and affinity of peptide antigen. The strength of TCR signaling drives a multitude of T cell functions from development to differentiation. CD8 T cells differentiate into ... ...

    Abstract Signaling downstream of the T cell receptor (TCR) is directly regulated by the dose and affinity of peptide antigen. The strength of TCR signaling drives a multitude of T cell functions from development to differentiation. CD8 T cells differentiate into a diverse pool of effector and memory cells after activation, a process that is critical for pathogen clearance and is highly regulated by TCR signal strength. T cells rapidly alter their gene expression upon activation. Multiple signaling pathways downstream of the TCR activate transcription factors, which are critical for this process. The dynamics between proximal TCR signaling, transcription factor activation and CD8 T cell function are discussed here. We propose that inducible T cell kinase (ITK) acts as a rheostat for gene expression. This unique regulation of TCR signaling by ITK provides a possible signaling mechanism for the promotion of a diverse T cell repertoire in response to pathogen.
    Language English
    Publishing date 2016
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2606827-8
    ISSN 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2016.00076
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  10. Article: Patent expiry and pharmaceutical market opportunities at the nexus of pricing and innovation policy

    Jain, Dipak C / Conley, James G

    Innovation and marketing in the pharmaceutical industry : emerging practices, research, and policies , p. 255-285

    2014  , Page(s) 255–285

    Author's details Dipak C. Jain and James G. Conley
    Keywords Patentrecht ; Innovationsmanagement ; Pharmazeutisches Produkt ; Produktentwicklung ; Wettbewerbsvorteil ; USA
    Language English
    Size Ill., graph. Darst.
    Publisher Springer
    Publishing place New York, NY [u.a.]
    Document type Article
    ISBN 978-1-4614-7800-3 ; 1-4614-7800-6
    Database ECONomics Information System

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