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Article ; Online: In Vitro Profiling of Potential Fish Probiotics, Enterococcus hirae Strains, Isolated from Jade Perch, and Safety Properties Assessed Using Whole Genome Sequencing.

Li, Wenrui / Lim, Chiun Hao / Zhao, Zhongtian / Wang, Yulan / Conway, Patricia Lynne / Loo, Say Chye Joachim

2024

Abstract: The demands of intensified aquaculture production and escalating disease prevalence underscore the need for efficacious probiotic strategies to enhance fish health. This study focused on isolating and characterising potential probiotics from the gut ... ...

Abstract	The demands of intensified aquaculture production and escalating disease prevalence underscore the need for efficacious probiotic strategies to enhance fish health. This study focused on isolating and characterising potential probiotics from the gut microbiota of the emerging aquaculture species jade perch (Scortum barcoo). Eighty-seven lactic acid bacteria and 149 other bacteria were isolated from the digestive tract of five adult jade perch. The screening revealed that 24 Enterococcus hirae isolates inhibited the freshwater pathogens Aeromonas sobria and Streptococcus iniae. Co-incubating E. hirae with the host gut suspensions demonstrated a two- to five-fold increase in the size of growth inhibition zones compared to the results when using gut suspensions from tilapia (a non-host), indicating host-specificity. Genome analysis of the lead isolate, E. hirae R44, predicted the presence of antimicrobial compounds like enterolysin A, class II lanthipeptide, and terpenes, which underlay its antibacterial attributes. Isolate R44 exhibited desirable probiotic characteristics, including survival at pH values within the range of 3 to 12, bile tolerance, antioxidant activity, ampicillin sensitivity, and absence of transferable antimicrobial resistance genes and virulence factors commonly associated with hospital Enterococcus strains (IS16, hylEfm, and esp). This study offers a foundation for sourcing host-adapted probiotics from underexplored aquaculture species. Characterisation of novel probiotics like E. hirae R44 can expedite the development of disease mitigation strategies to support aquaculture intensification.
Language	English
Publishing date	2024-03-18
Publishing country	United States
Document type	Journal Article
ZDB-ID	2487792-X
ISSN	1867-1314 ; 1867-1306
ISSN (online)	1867-1314
ISSN	1867-1306
DOI	10.1007/s12602-024-10244-0
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Article ; Online: Lactobacillus fermentum

Esvaran, Meera / Conway, Patricia L

Nutrients

2019 Volume 11, Issue 4

Abstract: ... ...

Abstract	Lactobacillus
MeSH term(s)	Animals ; Arthritis, Experimental/chemically induced ; Arthritis, Experimental/therapy ; Collagen Type II/toxicity ; Inflammation/drug therapy ; Joints/drug effects ; Joints/pathology ; Lactobacillus fermentum/physiology ; Male ; Mice ; Mice, Inbred DBA ; Probiotics/pharmacology
Chemical Substances	Collagen Type II
Language	English
Publishing date	2019-04-05
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2518386-2
ISSN	2072-6643 ; 2072-6643
ISSN (online)	2072-6643
ISSN	2072-6643
DOI	10.3390/nu11040785
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Article: <i>Lactobacillus fermentum</i> PC1 has the Capacity to Attenuate Joint Inflammation in Collagen-Induced Arthritis in DBA/1 Mice

Esvaran, Meera / Conway, Patricia L

Nutrients. 2019 Apr. 05, v. 11, no. 4

2019

Abstract: Lactobacillus strains have shown efficacy in attenuating inflammation. This study evaluated the potential of Lactobacillus fermentum PC1 for the treatment of rheumatoid arthritis (RA) using a murine model of collagen-induced arthritis. On Day 1, healthy ... ...

Abstract	Lactobacillus strains have shown efficacy in attenuating inflammation. This study evaluated the potential of Lactobacillus fermentum PC1 for the treatment of rheumatoid arthritis (RA) using a murine model of collagen-induced arthritis. On Day 1, healthy DBA/1 mice (six to eight weeks of age) were immunized, with 100 μg of Chicken Type 11 collagen emulsified in complete Freund’s adjuvant (CFA) by intradermal injection, at the base of the tail. On Day 21, the mice were immunized intraperitoneally with 100 μg of Bovine Type11 collagen in phosphate buffered saline (PBS). On Day 28, the mice were immunized intraperitoneally with 50 μg of lipopolysaccharide (LPS). Viable L. fermentum PC1 (1 × 109 colony forming units) was given daily from Day two until the end of the experiment. From Day 21 onwards, the mice were monitored daily for clinical signs of arthritis. On Day 44, the experiment was terminated. Paws were obtained for histology and serum for cytokine assays. L. fermentum PC1-fed mice had significantly reduced paw inflammation as well as decreased synovial infiltration and less cartilage damage. Circulating serum cytokine profiles revealed decreased IL-12 and increased anti-inflammatory cytokines, namely IL-4 and IL-10. Thus, early administration of L. fermentum PC1 could prove to be a valuable therapeutic agent in the management of RA.
Keywords	Lactobacillus fermentum ; adjuvants ; animal models ; blood serum ; cartilage ; cattle ; chickens ; collagen ; histology ; inflammation ; interleukin-10 ; interleukin-12 ; interleukin-4 ; lipopolysaccharides ; mice ; phosphates ; rheumatoid arthritis ; signs and symptoms (animals and humans) ; tail ; therapeutics
Language	English
Dates of publication	2019-0405
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2518386-2
ISSN	2072-6643
ISSN	2072-6643
DOI	10.3390/nu11040785
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Article: Honeys with anti-inflammatory capacity can alter the elderly gut microbiota in an ex vivo gut model

Wu, Daoyan / Chen, Liwei / Teh, Jean / Sim, Edric / Schlundt, Joergen / Conway, Patricia L.

Food chemistry. 2022 May 14,

2022

Abstract: The anti-inflammatory effect of different sourced honeys and the impact on elderly gut microbiota were studied in terms of chemical compositions, anti-inflammatory effect and gut microbiota modulating capacities. All four honeys suppressed the production ...

Abstract	The anti-inflammatory effect of different sourced honeys and the impact on elderly gut microbiota were studied in terms of chemical compositions, anti-inflammatory effect and gut microbiota modulating capacities. All four honeys suppressed the production of pro-inflammatory markers NO, IL-1β and IL-6 induced by lipopolysaccharide and promoted the expression of anti-inflammatory cytokines IL-10 in RAW 264.7 cells. Moreover, in the ex vivo batch gut model using elderly fecal microbiota (referred to as microcosm), it was showed that the addition of honeys increased the abundance of beneficial lactobacilli, decreased the abundance of potentially harmful Gram negative enteric bacteria, and exerted a beneficial effect on the production of short chain fatty acids. The concentration of gallic acid in honeys was positively correlated with the expression level of IL-10 and the abundance of lactobacilli. These findings indicate honeys with anti-inflammatory capacity have great potential for regulating the elderly gut microbiota which would lead to health benefits.
Keywords	Lactobacillus ; anti-inflammatory activity ; digestive system ; elderly ; food chemistry ; gallic acid ; interleukin-10 ; interleukin-6 ; intestinal microorganisms ; lipopolysaccharides ; models
Language	English
Dates of publication	2022-0514
Publishing place	Elsevier Ltd
Document type	Article
Note	Pre-press version
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2022.133229
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Article ; Online: Lactobacilli can attenuate inflammation in mouse macrophages exposed to polyethylene particles in vitro.

Esvaran, Meera / Conway, Patricia L

BMC research notes

2018 Volume 11, Issue 1, Page(s) 567

Abstract: Objective: It is well established that polyethylene (PE) wear particles induce macrophage production of cytokines and mediators associated with the pathogenesis of inflammatory osteolysis. The objective of this study was to examine the potential of ... ...

Abstract	Objective: It is well established that polyethylene (PE) wear particles induce macrophage production of cytokines and mediators associated with the pathogenesis of inflammatory osteolysis. The objective of this study was to examine the potential of three Lactobacillus strains to attenuate the TNF-α cytokine response of macrophages exposed to Ceridust 3615 PE particles. An in vitro experimental model using the RAW 246.7 macrophage cell line and PE particles was utilized. Results: Lactobacillus strains were found to modulate the cytokines in a strain and dose specific manner. Only the Lactobacillus acidophilus strain that was tested was able to attenuate PE particle-induced TNF-α production by RAW 246.7 macrophages. This effect was independent of IL-10 cytokine levels since all three strains of lactobacilli yielded comparable levels of IL-10. It was concluded that some, but not all, Lactobacillus strains may be useful in reducing the risk of inflammatory osteolysis and that further studies in appropriate in vivo models are warranted. Furthermore, this in vitro model can be used to evaluate the inflammatory potential of new materials being tested for use as joint implants.
MeSH term(s)	Animals ; Cytokines ; Inflammation ; Lactobacillus ; Macrophages/drug effects ; Mice ; Osteolysis ; Polyethylene/toxicity ; Tumor Necrosis Factor-alpha
Chemical Substances	Cytokines ; Tumor Necrosis Factor-alpha ; Polyethylene (9002-88-4)
Language	English
Publishing date	2018-08-08
Publishing country	England
Document type	Journal Article
ZDB-ID	2413336-X
ISSN	1756-0500 ; 1756-0500
ISSN (online)	1756-0500
ISSN	1756-0500
DOI	10.1186/s13104-018-3676-z
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Article ; Online: Honeys with anti-inflammatory capacity can alter the elderly gut microbiota in an ex vivo gut model.

Wu, Daoyan / Chen, Liwei / Teh, Jean / Sim, Edric / Schlundt, Joergen / Conway, Patricia L

Food chemistry

2022 Volume 392, Page(s) 133229

Abstract	The anti-inflammatory effect of different sourced honeys and the impact on elderly gut microbiota were studied in terms of chemical compositions, anti-inflammatory effect and gut microbiota modulating capacities. All four honeys suppressed the production of pro-inflammatory markers NO, IL-1β and IL-6 induced by lipopolysaccharide and promoted the expression of anti-inflammatory cytokines IL-10 in RAW 264.7 cells. Moreover, in the ex vivo batch gut model using elderly fecal microbiota (referred to as microcosm), it was showed that the addition of honeys increased the abundance of beneficial lactobacilli, decreased the abundance of potentially harmful Gram negative enteric bacteria, and exerted a beneficial effect on the production of short chain fatty acids. The concentration of gallic acid in honeys was positively correlated with the expression level of IL-10 and the abundance of lactobacilli. These findings indicate honeys with anti-inflammatory capacity have great potential for regulating the elderly gut microbiota which would lead to health benefits.
MeSH term(s)	Aged ; Anti-Inflammatory Agents/metabolism ; Anti-Inflammatory Agents/pharmacology ; Fatty Acids, Volatile/metabolism ; Gastrointestinal Microbiome ; Honey ; Humans ; Interleukin-10/genetics ; Interleukin-10/metabolism ; Lactobacillus/metabolism
Chemical Substances	Anti-Inflammatory Agents ; Fatty Acids, Volatile ; Interleukin-10 (130068-27-8)
Language	English
Publishing date	2022-05-18
Publishing country	England
Document type	Journal Article
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2022.133229
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Liquorilactobacillus satsumensis from water kefir yields α-glucan polysaccharides with prebiotic and synbiotic qualities.

Tan, Li Ling / Ngiam, Jia Jun / Sim, Edric Shao Zhe / Conway, Patricia Lynne / Loo, Say Chye Joachim

Carbohydrate polymers

2022 Volume 290, Page(s) 119515

Abstract: Exopolysaccharides from water kefir grains are a potential source of novel, food-safe and functional materials. Herein, prebiotic properties of polysaccharides produced by water kefir-derived Liquorilactobacillus satsumensis bacteria were explored. ... ...

Abstract	Exopolysaccharides from water kefir grains are a potential source of novel, food-safe and functional materials. Herein, prebiotic properties of polysaccharides produced by water kefir-derived Liquorilactobacillus satsumensis bacteria were explored. Strains were cultured in sucrose-supplemented media for exopolysaccharides production, and partial hydrolysis was performed to yield shorter chain polysaccharides. Structural characterization revealed that hydrolyzed polysaccharides were branched glucans comprising α-1,6 bonds and α-1,3/α-1,4 branching, with molecular weight of ~10 kDa. Hydrolyzed polysaccharides demonstrated selective utilization by probiotics, but not by pathogens, and were non-digestible by human digestive enzymes. Particularly, hydrolyzed polysaccharides were fermentable by kefir-derived probiotics, and these were combined in a novel kefir synbiotic formulation. Using large bowel simulated conditions, it was demonstrated that hydrolyzed polysaccharides and kefir synbiotics promoted Bacteroidetes abundance, and increased acetate, propionate, and butyrate concentrations. Overall, hydrolyzed glucans from Liquorilactobacillus satsumensis have prebiotic properties with enhanced benefits in a synbiotic when combined with kefir probiotics.
MeSH term(s)	Glucans ; Humans ; Kefir/microbiology ; Lactobacillus ; Polysaccharides ; Prebiotics ; Probiotics/chemistry ; Synbiotics ; Water
Chemical Substances	Glucans ; Polysaccharides ; Prebiotics ; Water (059QF0KO0R)
Language	English
Publishing date	2022-04-22
Publishing country	England
Document type	Journal Article
ZDB-ID	1501516-6
ISSN	1879-1344 ; 0144-8617
ISSN (online)	1879-1344
ISSN	0144-8617
DOI	10.1016/j.carbpol.2022.119515
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Article ; Online: Surface-layer protein is a public-good matrix exopolymer for microbial community organisation in environmental anammox biofilms.

Wong, Lan Li / Lu, Yang / Ho, James Chin Shing / Mugunthan, Sudarsan / Law, Yingyu / Conway, Patricia / Kjelleberg, Staffan / Seviour, Thomas

The ISME journal

2023 Volume 17, Issue 6, Page(s) 803–812

Abstract: Extracellular polymeric substances (EPS) are core biofilm components, yet how they mediate interactions within and contribute to the structuring of biofilms is largely unknown, particularly for non-culturable microbial communities that predominate in ... ...

Abstract	Extracellular polymeric substances (EPS) are core biofilm components, yet how they mediate interactions within and contribute to the structuring of biofilms is largely unknown, particularly for non-culturable microbial communities that predominate in environmental habitats. To address this knowledge gap, we explored the role of EPS in an anaerobic ammonium oxidation (anammox) biofilm. An extracellular glycoprotein, BROSI_A1236, from an anammox bacterium, formed envelopes around the anammox cells, supporting its identification as a surface (S-) layer protein. However, the S-layer protein also appeared at the edge of the biofilm, in close proximity to the polysaccharide-coated filamentous Chloroflexi bacteria but distal to the anammox bacterial cells. The Chloroflexi bacteria assembled into a cross-linked network at the edge of the granules and surrounding anammox cell clusters, with the S-layer protein occupying the space around the Chloroflexi. The anammox S-layer protein was also abundant at junctions between Chloroflexi cells. Thus, the S-layer protein is likely transported through the matrix as an EPS and also acts as an adhesive to facilitate the assembly of filamentous Chloroflexi into a three-dimensional biofilm lattice. The spatial distribution of the S-layer protein within the mixed species biofilm suggests that it is a "public-good" EPS, which facilitates the assembly of other bacteria into a framework for the benefit of the biofilm community, and enables key syntrophic relationships, including anammox.
MeSH term(s)	Extracellular Polymeric Substance Matrix/metabolism ; Membrane Proteins ; Anaerobic Ammonia Oxidation ; Bioreactors ; Anaerobiosis ; Oxidation-Reduction ; Biofilms ; Bacteria/genetics ; Bacteria/metabolism ; Chloroflexi/metabolism ; Nitrogen/metabolism ; Sewage ; Ammonium Compounds/metabolism
Chemical Substances	Membrane Proteins ; Nitrogen (N762921K75) ; Sewage ; Ammonium Compounds
Language	English
Publishing date	2023-03-04
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2406536-5
ISSN	1751-7370 ; 1751-7362
ISSN (online)	1751-7370
ISSN	1751-7362
DOI	10.1038/s41396-023-01388-y
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Article: Liquorilactobacillus satsumensis from water kefir yields α-glucan polysaccharides with prebiotic and synbiotic qualities

Tan, Li Ling / Ngiam, Jia Jun / Sim, Edric Shao Zhe / Conway, Patricia Lynne / Loo, Say Chye Joachim

Carbohydrate polymers. 2022 Apr. 19,

2022

Abstract	Exopolysaccharides from water kefir grains are a potential source of novel, food-safe and functional materials. Herein, prebiotic properties of polysaccharides produced by water kefir-derived Liquorilactobacillus satsumensis bacteria were explored. Strains were cultured in sucrose-supplemented media for exopolysaccharides production, and partial hydrolysis was performed to yield shorter chain polysaccharides. Structural characterization revealed that hydrolyzed polysaccharides were branched glucans comprising α-1,6 bonds and α-1,3/α-1,4 branching, with molecular weight of ~10 kDa. Hydrolyzed polysaccharides demonstrated selective utilization by probiotics, but not by pathogens, and were non-digestible by human digestive enzymes. Particularly, hydrolyzed polysaccharides were fermentable by kefir-derived probiotics, and these were combined in a novel kefir synbiotic formulation. Using large bowel simulated conditions, it was demonstrated that hydrolyzed polysaccharides and kefir synbiotics promoted Bacteroidetes abundance, and increased acetate, propionate, and butyrate concentrations. Overall, hydrolyzed glucans from Liquorilactobacillus satsumensis have prebiotic properties with enhanced benefits in a synbiotic when combined with kefir probiotics.
Keywords	Bacteroidetes ; acetates ; butyrates ; exopolysaccharides ; glucans ; humans ; hydrolysis ; kefir ; molecular weight ; prebiotics ; probiotics ; propionic acid
Language	English
Dates of publication	2022-0419
Publishing place	Elsevier Ltd
Document type	Article
Note	Pre-press version
ZDB-ID	1501516-6
ISSN	1879-1344 ; 0144-8617
ISSN (online)	1879-1344
ISSN	0144-8617
DOI	10.1016/j.carbpol.2022.119515
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Article: Development of a Dairy-Free Fermented Oat-Based Beverage With Enhanced Probiotic and Bioactive Properties.

Chen, Liwei / Wu, Daoyan / Schlundt, Joergen / Conway, Patricia L

Frontiers in microbiology

2020 Volume 11, Page(s) 609734

Abstract: Lactobacillus ... ...

Abstract	Lactobacillus fermentum
Language	English
Publishing date	2020-12-03
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2020.609734
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