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  1. Article ; Online: Cytomegalovirus: Another Source of Antibody-mediated Graft Injury?

    Cook, Charles H

    Transplantation

    2020  Volume 105, Issue 8, Page(s) 1653–1654

    MeSH term(s) Cytomegalovirus ; Cytomegalovirus Infections/diagnosis ; Graft Survival ; Humans
    Language English
    Publishing date 2020-10-28
    Publishing country United States
    Document type Journal Article ; Comment
    ZDB-ID 208424-7
    ISSN 1534-6080 ; 0041-1337
    ISSN (online) 1534-6080
    ISSN 0041-1337
    DOI 10.1097/TP.0000000000003549
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Cytomegalovirus reactivation: Another reason to minimize graft ischemia/reperfusion.

    Cook, Charles H

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

    2019  Volume 19, Issue 9, Page(s) 2399–2400

    MeSH term(s) Animals ; Cytomegalovirus ; Cytomegalovirus Infections ; Disease Models, Animal ; Kidney Transplantation ; Mice ; Reperfusion
    Language English
    Publishing date 2019-07-18
    Publishing country United States
    Document type Editorial ; Comment
    ZDB-ID 2060594-8
    ISSN 1600-6143 ; 1600-6135
    ISSN (online) 1600-6143
    ISSN 1600-6135
    DOI 10.1111/ajt.15504
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 5542: Show issues Location:
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  3. Article ; Online: nanoString evaluation of murine Cytomegalovirus transcription in vivo and in vitro

    Griessl, Marion / Gutknecht, Michael / Juranić-Lisnić, Vanda / Cook, Charles H.

    Journal of Virological Methods. 2022 Mar., v. 301 p.114436-

    2022  

    Abstract: Next Generation Sequencing allows for deep analysis of transcriptional activity in cells and tissues, however it is still a cost intensive method that demands well versed data handling. Reverse transcription quantitative PCR (RT-qPCR) is the most ... ...

    Abstract Next Generation Sequencing allows for deep analysis of transcriptional activity in cells and tissues, however it is still a cost intensive method that demands well versed data handling. Reverse transcription quantitative PCR (RT-qPCR) is the most commonly used method to measure gene expression levels, however the information gathered is quite small in comparison to NGS. A newer method called nanoString allows for highly multiplexed gene expression analysis by detecting mRNAs without the use of enzymes for reverse transcription or amplification even for single cells or low input material. The method can be done in 1.5 days and data are quickly analyzed by the accompanied user friendly software. Our aim was to investigate this new method and compare it to the existing alternatives, while investigating murine Cytomegalovirus (mCMV) infection and latency. mCMV infected murine embryonic fibroblasts (MEF), lung and salivary glands from BALB/c mice were evaluated at different stages of infection. A set of 30 custom designed nanoString probes were tested, 20 probes specific for mCMV genes, 6 probes for host genes known to be influenced by viral infection and 4 reference gene specific probes. nanoString counts were compared to published RNA-Seq RPKM. We found that nanoString can be used for analysis of cytomegalovirus gene expression during acute infection in vitro and in vivo, both for virus specific and host genes. Although some transcripts show different expression rates in comparison to NGS data, the most abundant transcripts are comparable. When tissues are infected, there are significantly fewer transcripts than in MEFs, and consistent with previous work there are significant differences in relevant abundance between MEF and tissues. We were unable to detect our viral transcripts of interest in latently infected tissue. For viruses with annotated transcriptomes, nanoString allows simultaneous quantitation of multiple virus and host genes. One huge advantage of the platform is rapid turnaround and simplicity of analysis. It should prove to be very useful to explore host virus interactions during acute infection, but it is unclear if it has adequate sensitivity for analysis during latency in immunocompetent mice.
    Keywords Muromegalovirus ; computer software ; fibroblasts ; gene expression ; genes ; lungs ; mice ; reverse transcriptase polymerase chain reaction ; reverse transcription ; sequence analysis ; transcriptome ; viruses ; Murine cytomegalovirus ; nanoString ; Viral transcription
    Language English
    Dates of publication 2022-03
    Publishing place Elsevier B.V.
    Document type Article ; Online
    ZDB-ID 8013-5
    ISSN 1879-0984 ; 0166-0934
    ISSN (online) 1879-0984
    ISSN 0166-0934
    DOI 10.1016/j.jviromet.2021.114436
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Cytomegalovirus Encephalopathy during Brain Tumor Irradiation.

    Lawler, Sean E / Chiocca, E Antonio / Cook, Charles H

    Clinical cancer research : an official journal of the American Association for Cancer Research

    2020  Volume 26, Issue 13, Page(s) 3077–3078

    Abstract: Some patients with brain cancer show extremely short survival postradiochemotherapy treatment for unknown reasons. Recent work shows that this is closely linked to encephalopathy associated with reactivation of latent cytomegalovirus in the host. ... ...

    Abstract Some patients with brain cancer show extremely short survival postradiochemotherapy treatment for unknown reasons. Recent work shows that this is closely linked to encephalopathy associated with reactivation of latent cytomegalovirus in the host. Importantly, survival can be enhanced by treatment with antiviral drugs.
    MeSH term(s) Brain Neoplasms/radiotherapy ; Chemoradiotherapy ; Cytomegalovirus ; Cytomegalovirus Infections ; Humans ; Virus Activation
    Language English
    Publishing date 2020-04-10
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Comment
    ZDB-ID 1225457-5
    ISSN 1557-3265 ; 1078-0432
    ISSN (online) 1557-3265
    ISSN 1078-0432
    DOI 10.1158/1078-0432.CCR-20-0646
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: nanoString evaluation of murine Cytomegalovirus transcription in vivo and in vitro.

    Griessl, Marion / Gutknecht, Michael / Juranić-Lisnić, Vanda / Cook, Charles H

    Journal of virological methods

    2021  Volume 301, Page(s) 114436

    Abstract: Background: Next Generation Sequencing allows for deep analysis of transcriptional activity in cells and tissues, however it is still a cost intensive method that demands well versed data handling. Reverse transcription quantitative PCR (RT-qPCR) is the ...

    Abstract Background: Next Generation Sequencing allows for deep analysis of transcriptional activity in cells and tissues, however it is still a cost intensive method that demands well versed data handling. Reverse transcription quantitative PCR (RT-qPCR) is the most commonly used method to measure gene expression levels, however the information gathered is quite small in comparison to NGS. A newer method called nanoString allows for highly multiplexed gene expression analysis by detecting mRNAs without the use of enzymes for reverse transcription or amplification even for single cells or low input material. The method can be done in 1.5 days and data are quickly analyzed by the accompanied user friendly software. Our aim was to investigate this new method and compare it to the existing alternatives, while investigating murine Cytomegalovirus (mCMV) infection and latency.
    Methods: mCMV infected murine embryonic fibroblasts (MEF), lung and salivary glands from BALB/c mice were evaluated at different stages of infection. A set of 30 custom designed nanoString probes were tested, 20 probes specific for mCMV genes, 6 probes for host genes known to be influenced by viral infection and 4 reference gene specific probes. nanoString counts were compared to published RNA-Seq RPKM.
    Results: We found that nanoString can be used for analysis of cytomegalovirus gene expression during acute infection in vitro and in vivo, both for virus specific and host genes. Although some transcripts show different expression rates in comparison to NGS data, the most abundant transcripts are comparable. When tissues are infected, there are significantly fewer transcripts than in MEFs, and consistent with previous work there are significant differences in relevant abundance between MEF and tissues. We were unable to detect our viral transcripts of interest in latently infected tissue.
    Conclusions: For viruses with annotated transcriptomes, nanoString allows simultaneous quantitation of multiple virus and host genes. One huge advantage of the platform is rapid turnaround and simplicity of analysis. It should prove to be very useful to explore host virus interactions during acute infection, but it is unclear if it has adequate sensitivity for analysis during latency in immunocompetent mice.
    MeSH term(s) Animals ; Cytomegalovirus/genetics ; Cytomegalovirus Infections ; Mice ; Mice, Inbred BALB C ; Muromegalovirus/genetics ; Transcriptome
    Language English
    Publishing date 2021-12-17
    Publishing country Netherlands
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 8013-5
    ISSN 1879-0984 ; 0166-0934
    ISSN (online) 1879-0984
    ISSN 0166-0934
    DOI 10.1016/j.jviromet.2021.114436
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Antiviral prophylaxis of cytomegalovirus reactivation in immune competent patients-the jury remains out.

    Mansfield, Sara A / Cook, Charles H

    Journal of thoracic disease

    2017  Volume 9, Issue 8, Page(s) 2221–2223

    Language English
    Publishing date 2017-08-30
    Publishing country China
    Document type Editorial ; Comment
    ZDB-ID 2573571-8
    ISSN 2077-6624 ; 2072-1439
    ISSN (online) 2077-6624
    ISSN 2072-1439
    DOI 10.21037/jtd.2017.06.130
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Addressing Limitations in Case-Control Study of Patients Undergoing Resuscitative Endovascular Balloon Occlusion of the Aorta.

    Yuan, William / Cook, Charles H / Brat, Gabriel A

    JAMA surgery

    2019  Volume 154, Issue 12, Page(s) 1167

    MeSH term(s) Aorta ; Balloon Occlusion ; Case-Control Studies ; Humans ; Resuscitation
    Language English
    Publishing date 2019-10-16
    Publishing country United States
    Document type Letter ; Comment
    ZDB-ID 2701841-6
    ISSN 2168-6262 ; 2168-6254
    ISSN (online) 2168-6262
    ISSN 2168-6254
    DOI 10.1001/jamasurg.2019.2744
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Impact of cytomegalovirus load on host response to sepsis.

    Marandu, Thomas / Dombek, Michael / Cook, Charles H

    Medical microbiology and immunology

    2019  Volume 208, Issue 3-4, Page(s) 295–303

    Abstract: There is a decades old association between cytomegalovirus reactivation and sepsis in immune-competent hosts. Much has been learned about this relationship, which has been described as bidirectional, meaning that the virus incites and is incited by the ... ...

    Abstract There is a decades old association between cytomegalovirus reactivation and sepsis in immune-competent hosts. Much has been learned about this relationship, which has been described as bidirectional, meaning that the virus incites and is incited by the host's inflammatory response. More recent work has suggested that chronic viral infection leaves the host with exaggerated immunity to bacterial infections. In this review, the relationship between CMV and host responses to sepsis are reviewed, with particular attention to the impact that tissue viral load contributes to this phenomenon.
    MeSH term(s) Cytomegalovirus/growth & development ; Cytomegalovirus Infections/complications ; Sepsis/complications ; Sepsis/pathology ; Viral Load ; Virus Activation
    Language English
    Publishing date 2019-04-11
    Publishing country Germany
    Document type Journal Article ; Review
    ZDB-ID 120933-4
    ISSN 1432-1831 ; 0300-8584
    ISSN (online) 1432-1831
    ISSN 0300-8584
    DOI 10.1007/s00430-019-00603-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Colon Resection With End Colostomy in a Patient With a History of Imperforate Anus.

    Misra, Asish / Cook, Charles H / Cataldo, Thomas E / Narula, Nisha

    The American surgeon

    2020  Volume 89, Issue 3, Page(s) 460–461

    MeSH term(s) Humans ; Anus, Imperforate/complications ; Anus, Imperforate/surgery ; Colostomy ; Colon/surgery ; Colectomy ; Patients
    Language English
    Publishing date 2020-11-10
    Publishing country United States
    Document type Journal Article
    ZDB-ID 202465-2
    ISSN 1555-9823 ; 0003-1348
    ISSN (online) 1555-9823
    ISSN 0003-1348
    DOI 10.1177/0003134820953776
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Cytomegalovirus reactivation and mortality during critical illness: a $64,000 question.

    Cook, Charles H

    Critical care medicine

    2009  Volume 37, Issue 8, Page(s) 2475–2476

    MeSH term(s) Critical Illness/mortality ; Cytomegalovirus Infections/epidemiology ; Cytomegalovirus Infections/mortality ; Humans ; Intensive Care Units ; Prevalence ; Virus Activation
    Language English
    Publishing date 2009-08
    Publishing country United States
    Document type Comment ; Editorial
    ZDB-ID 197890-1
    ISSN 1530-0293 ; 0090-3493
    ISSN (online) 1530-0293
    ISSN 0090-3493
    DOI 10.1097/CCM.0b013e3181ad932e
    Database MEDical Literature Analysis and Retrieval System OnLINE

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