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  1. Article: Three-dimensional reconstruction of in vivo bioluminescent sources based on multispectral imaging.

    Kuo, Chaincy / Coquoz, Olivier / Troy, Tamara L / Xu, Heng / Rice, Brad W

    Journal of biomedical optics

    2006  Volume 12, Issue 2, Page(s) 24007

    Abstract: A new method is described for obtaining a 3-D reconstruction of a bioluminescent light source distribution inside a living animal subject, from multispectral images of the surface light emission acquired on charge-coupled device (CCD) camera. The method ... ...

    Abstract A new method is described for obtaining a 3-D reconstruction of a bioluminescent light source distribution inside a living animal subject, from multispectral images of the surface light emission acquired on charge-coupled device (CCD) camera. The method uses the 3-D surface topography of the animal, which is obtained from a structured light illumination technique. The forward model of photon transport is based on the diffusion approximation in homogeneous tissue with a local planar boundary approximation for each mesh element, allowing rapid calculation of the forward Green's function kernel. Absorption and scattering properties of tissue are measured a priori as input to the algorithm. By using multispectral images, 3-D reconstructions of luminescent sources can be derived from images acquired from only a single view. As a demonstration, the reconstruction technique is applied to determine the location and brightness of a source embedded in a homogeneous phantom subject in the shape of a mouse. The technique is then evaluated with real mouse models in which calibrated sources are implanted at known locations within living tissue. Finally, reconstructions are demonstrated in a PC3M-luc (prostate tumor line) metastatic tumor model in nude mice.
    MeSH term(s) Animals ; Image Interpretation, Computer-Assisted/methods ; Imaging, Three-Dimensional/methods ; Luminescent Proteins/analysis ; Male ; Mice ; Microscopy, Fluorescence, Multiphoton/methods ; Prostatic Neoplasms/pathology ; Whole Body Imaging/methods
    Chemical Substances Luminescent Proteins
    Language English
    Publishing date 2006-12-08
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1309154-2
    ISSN 1083-3668
    ISSN 1083-3668
    DOI 10.1117/1.2717898
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4699: Show issues Location:
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  2. Article: Emission spectra of bioluminescent reporters and interaction with mammalian tissue determine the sensitivity of detection in vivo.

    Zhao, Hui / Doyle, Timothy C / Coquoz, Olivier / Kalish, Flora / Rice, Bradley W / Contag, Christopher H

    Journal of biomedical optics

    2004  Volume 10, Issue 4, Page(s) 41210

    Abstract: In vivo bioluminescence imaging depends on light emitted by luciferases in the body overcoming the effect of tissue attenuation. Understanding this relationship is essential for detection and quantification of signal. We have studied four codon optimized ...

    Abstract In vivo bioluminescence imaging depends on light emitted by luciferases in the body overcoming the effect of tissue attenuation. Understanding this relationship is essential for detection and quantification of signal. We have studied four codon optimized luciferases with different emission spectra, including enzymes from firefly (FLuc), click beetle (CBGr68, CBRed) and Renilla reniformins (hRLuc). At 25 degrees C, the in vitro lambda(max) of these reporters are 578, 543, 615, and 480 nm, respectively; at body temperature, 37 degrees C, the brightness increases and the firefly enzyme demonstrates a 34-nm spectral red shift. Spectral shifts and attenuation due to tissue effects were evaluated using a series of 20-nm bandpass filters and a cooled charge-coupled device (CCD) camera. Attenuation increased and the spectra of emitted light was red shifted for signals originating from deeper within the body relative to superficial origins. The tissue attenuation of signals from CBGr68 and hRLuc was greater than from those of Fluc and CBRed. To further probe tissue effects, broad spectral emitters were created through gene fusions between CBGr68 and CBRed. These resulted in enzymes with broader emission spectra, featuring two peaks whose intensities are differentially affected by temperature and tissue depth. These spectral measurement data allow for improved understanding of how these reporters can be used in vivo and what they can reveal about biological processes in living subjects.
    MeSH term(s) Animals ; Female ; Gene Expression Profiling/methods ; Genes, Reporter/physiology ; Glioma/genetics ; Glioma/metabolism ; Luciferases/genetics ; Luciferases/metabolism ; Luminescent Measurements/methods ; Luminescent Proteins/genetics ; Luminescent Proteins/metabolism ; Mammals ; Mice ; Mice, Inbred BALB C ; Rats ; Reproducibility of Results ; Sensitivity and Specificity ; Spectrometry, Fluorescence/methods
    Chemical Substances Luminescent Proteins ; Luciferases (EC 1.13.12.-)
    Language English
    Publishing date 2004-10-18
    Publishing country United States
    Document type Comparative Study ; Evaluation Studies ; Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 1309154-2
    ISSN 1083-3668
    ISSN 1083-3668
    DOI 10.1117/1.2032388
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  3. Article: Reduction of Astrogliosis by Early Treatment of Pneumococcal Meningitis Measured by Simultaneous Imaging, In Vivo, of the Pathogen and Host Response

    Kadurugamuwa, Jagath L / Modi, Kshitij / Coquoz, Olivier / Rice, Brad / Smith, Steven / Contag, Pamela R / Purchio, Tony

    Infection and immunity. 2005 Dec., v. 73, no. 12

    2005  

    Abstract: We developed a method for simultaneous in vivo biophotonic monitoring of pneumococcal meningitis and the accompanying neuronal injury in live transgenic mice. Streptococcus pneumoniae engineered for bioluminescence (lux) was used for direct visualization ...

    Abstract We developed a method for simultaneous in vivo biophotonic monitoring of pneumococcal meningitis and the accompanying neuronal injury in live transgenic mice. Streptococcus pneumoniae engineered for bioluminescence (lux) was used for direct visualization of disease progression and antibiotic treatment in a mouse model of meningitis. The host response was monitored in transgenic mice containing an inducible firefly luciferase (luc) reporter gene under transcriptional control of the mouse glial fibrillary acidic protein (GFAP) promoter. Based on the different spectra of light emission and substrate requirements for lux and luc, we were able to separately monitor the two reporters using a highly sensitive in vivo imaging system. The level of neuronal damage and recovery following antibiotic treatment was dependent on the time of treatment. This model has potential for simultaneous multiparameter monitoring and testing of therapies that target the pathogen or host response to prevent neuronal injury and recovery.
    Keywords Streptococcus pneumoniae ; animal models ; antibiotics ; bioluminescence ; disease course ; image analysis ; luciferase ; meningitis ; mice ; monitoring ; pathogens ; reporter genes ; transcription (genetics)
    Language English
    Size p. 7836-7843.
    Publishing place American Society for Microbiology
    Document type Article
    ZDB-ID 218698-6
    ISSN 1098-5522 ; 0019-9567
    ISSN (online) 1098-5522
    ISSN 0019-9567
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

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  4. Article: Reduction of astrogliosis by early treatment of pneumococcal meningitis measured by simultaneous imaging, in vivo, of the pathogen and host response.

    Kadurugamuwa, Jagath L / Modi, Kshitij / Coquoz, Olivier / Rice, Brad / Smith, Steven / Contag, Pamela R / Purchio, Tony

    Infection and immunity

    2005  Volume 73, Issue 12, Page(s) 7836–7843

    Abstract: We developed a method for simultaneous in vivo biophotonic monitoring of pneumococcal meningitis and the accompanying neuronal injury in live transgenic mice. Streptococcus pneumoniae engineered for bioluminescence (lux) was used for direct visualization ...

    Abstract We developed a method for simultaneous in vivo biophotonic monitoring of pneumococcal meningitis and the accompanying neuronal injury in live transgenic mice. Streptococcus pneumoniae engineered for bioluminescence (lux) was used for direct visualization of disease progression and antibiotic treatment in a mouse model of meningitis. The host response was monitored in transgenic mice containing an inducible firefly luciferase (luc) reporter gene under transcriptional control of the mouse glial fibrillary acidic protein (GFAP) promoter. Based on the different spectra of light emission and substrate requirements for lux and luc, we were able to separately monitor the two reporters using a highly sensitive in vivo imaging system. The level of neuronal damage and recovery following antibiotic treatment was dependent on the time of treatment. This model has potential for simultaneous multiparameter monitoring and testing of therapies that target the pathogen or host response to prevent neuronal injury and recovery.
    MeSH term(s) Animals ; Anti-Bacterial Agents/therapeutic use ; Astrocytes/pathology ; Brain/microbiology ; Brain/pathology ; Disease Models, Animal ; Genes, Reporter ; Glial Fibrillary Acidic Protein/genetics ; Gliosis/drug therapy ; Gliosis/pathology ; Luciferases, Firefly/analysis ; Luciferases, Firefly/genetics ; Luminescent Agents/analysis ; Luminescent Measurements ; Meningitis, Pneumococcal/drug therapy ; Meningitis, Pneumococcal/microbiology ; Meningitis, Pneumococcal/pathology ; Mice ; Mice, Transgenic ; Promoter Regions, Genetic/genetics ; Streptococcus pneumoniae/genetics
    Chemical Substances Anti-Bacterial Agents ; Glial Fibrillary Acidic Protein ; Luminescent Agents ; Luciferases, Firefly (EC 1.13.12.7)
    Language English
    Publishing date 2005-12
    Publishing country United States
    Document type Journal Article
    ZDB-ID 218698-6
    ISSN 1098-5522 ; 0019-9567
    ISSN (online) 1098-5522
    ISSN 0019-9567
    DOI 10.1128/IAI.73.12.7836-7843.2005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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