LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 2 of total 2

Search options

  1. Article ; Online: Epidermal growth factor receptor promotes cerebral and retinal invasion by Toxoplasma gondii.

    Corcino, Yalitza Lopez / Portillo, Jose-Andres C / Subauste, Carlos S

    Scientific reports

    2019  Volume 9, Issue 1, Page(s) 669

    Abstract: Little is known about strategies used by pathogens to facilitate CNS invasion. Toxoplasma gondii reaches the CNS by circulating in blood within leukocytes or as extracellular tachyzoites. T. gondii induces EGFR signaling in vitro during invasion of ... ...

    Abstract Little is known about strategies used by pathogens to facilitate CNS invasion. Toxoplasma gondii reaches the CNS by circulating in blood within leukocytes or as extracellular tachyzoites. T. gondii induces EGFR signaling in vitro during invasion of mammalian cells. We examined the effects of endothelial cell EGFR on CNS invasion. Transgenic mice whose endothelial cells expressed a dominant negative (DN) EGFR (inhibits EGFR signaling) exhibited diminished parasite load and histopathology in the brain and retina after T. gondii infection. I.V. administration of infected leukocytes or extracellular tachyzoites led to reduced parasite loads in mice with DN EGFR. This was not explained by enhanced immunity or reduced leukocyte recruitment. Endothelial cell infection is key for CNS invasion. Parasite foci in brain endothelial cells were reduced by DN EGFR. DN EGFR in these cells led to recruitment of the autophagy protein LC3 around T. gondii and spontaneous parasite killing dependent on the autophagy protein ULK1 and lysosomal enzymes. The autophagy inhibitor 3-MA prevented DN EGFR mice from exhibiting reduced CNS invasion. Altogether, EGFR is a novel regulator of T. gondii invasion of neural tissue, enhancing invasion likely by promoting survival of the parasite within endothelial cells.
    MeSH term(s) Animals ; Autophagy ; Brain/parasitology ; Brain/pathology ; Endothelial Cells/metabolism ; Endothelial Cells/parasitology ; ErbB Receptors/genetics ; ErbB Receptors/metabolism ; Female ; Host-Parasite Interactions/physiology ; Immunity, Humoral ; Leukocytes/pathology ; Mice, Transgenic ; Parasite Load ; Retina/parasitology ; Retina/pathology ; Toxoplasma/pathogenicity ; Toxoplasmosis/immunology ; Toxoplasmosis/metabolism ; Toxoplasmosis/parasitology
    Chemical Substances EGFR protein, mouse (EC 2.7.10.1) ; ErbB Receptors (EC 2.7.10.1)
    Language English
    Publishing date 2019-01-24
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-018-36724-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: Nonhomologous End-Joining with Minimal Sequence Loss Is Promoted by the Mre11-Rad50-Nbs1-Ctp1 Complex in

    Li, Yanhui / Wang, Jinyu / Zhou, Gang / Lajeunesse, Michael / Le, Nga / Stawicki, Brittany N / Corcino, Yalitza Lopez / Berkner, Kathleen L / Runge, Kurt W

    Genetics

    2017  Volume 206, Issue 1, Page(s) 481–496

    Abstract: While the Mre11-Rad50-Nbs1 (MRN) complex has known roles in repair processes like homologous recombination and microhomology-mediated end-joining, its role in nonhomologous end-joining (NHEJ) is unclear ... ...

    Abstract While the Mre11-Rad50-Nbs1 (MRN) complex has known roles in repair processes like homologous recombination and microhomology-mediated end-joining, its role in nonhomologous end-joining (NHEJ) is unclear as
    MeSH term(s) Chromosomal Proteins, Non-Histone/genetics ; DNA/genetics ; DNA Breaks, Double-Stranded ; DNA End-Joining Repair/genetics ; DNA-Binding Proteins/genetics ; Endodeoxyribonucleases/genetics ; Exodeoxyribonucleases/genetics ; Multiprotein Complexes/genetics ; Schizosaccharomyces/genetics ; Schizosaccharomyces pombe Proteins/genetics
    Chemical Substances Chromosomal Proteins, Non-Histone ; Ctp1 protein, S pombe ; DNA-Binding Proteins ; Multiprotein Complexes ; Nbs1 protein, S pombe ; Rad50 protein, S pombe ; Schizosaccharomyces pombe Proteins ; DNA (9007-49-2) ; Endodeoxyribonucleases (EC 3.1.-) ; Exodeoxyribonucleases (EC 3.1.-) ; Mre11 protein, S pombe (EC 3.1.-) ; Pso2 protein, S pombe (EC 3.1.-)
    Language English
    Publishing date 2017-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2167-2
    ISSN 1943-2631 ; 0016-6731
    ISSN (online) 1943-2631
    ISSN 0016-6731
    DOI 10.1534/genetics.117.200972
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 767: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top