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  1. Article ; Online: Local details versus effective medium approximation: A study of diffusion in microfluidic random networks made from Voronoi tessellations.

    Ponce, Washington / Cordero, María Luisa

    Physical review. E

    2020  Volume 101, Issue 2-1, Page(s) 23110

    Abstract: We measured the effective diffusion coefficient in regions of microfluidic networks of controlled geometry using the fluorescence recovery after photobleaching (FRAP) technique. The geometry of the networks was based on Voronoi tessellations, and had ... ...

    Abstract We measured the effective diffusion coefficient in regions of microfluidic networks of controlled geometry using the fluorescence recovery after photobleaching (FRAP) technique. The geometry of the networks was based on Voronoi tessellations, and had varying characteristic length scale and porosity. For a fixed network, FRAP experiments were performed in regions of increasing size. Our results indicate that the boundary of the bleached region, and in particular the cumulative area of the channels that connect the bleached region to the rest of the network, are important in the measured value of the effective diffusion coefficient. We found that the statistical geometrical variations between different regions of the network decrease with the size of the bleached region as a power law, meaning that the statistical error of effective medium approximations decrease with the size of the studied medium with no characteristic length scale.
    Language English
    Publishing date 2020-03-13
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2844562-4
    ISSN 2470-0053 ; 2470-0045
    ISSN (online) 2470-0053
    ISSN 2470-0045
    DOI 10.1103/PhysRevE.101.023110
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: First releases of hatchery-produced Senegal sole (Solea senegalensis), brill (Scophthalmus rhombus), and wedge sole (Dicologoglossa cuneata) juveniles in the South-western Spanish coast

    Herrera, Marcelino / Rodiles, Ana / Salamanca, Natalia / Navas, Jose Ignacio / Cordero, María Luisa / Hachero, Ismael

    Hydrobiologia. 2023 Jan., v. 850, no. 1 p.203-214

    2023  

    Abstract: The regression of fisheries in the Gulf of Cadiz is evident since current fish catches are 33% of that 30 years before. Consequently, some initiatives for the replenishment of exhausted wild stocks are welcome. The objective of the present work is to ... ...

    Abstract The regression of fisheries in the Gulf of Cadiz is evident since current fish catches are 33% of that 30 years before. Consequently, some initiatives for the replenishment of exhausted wild stocks are welcome. The objective of the present work is to describe and analyse the results coming from the first flatfish stock enhancements in Andalusia. A total of 3189 fish from three flatfish species: Senegal sole (Solea senegalensis Kaup), wedge sole (Dicologlossa cuneata Moreau), and brill (Scophthalmus rhombus Linnaeus) were tagged and released. Several variables were calculated through the data analysis of recovered fish. Some variables were calculated only for Senegal soles since wedge sole and brill recaptures were not significant. The Senegal sole recapture rate was 2.71 ± 0.72, similar to other published data, the recapture rates for bigger fish being higher though not significant. No significant differences were detected for distance, time, growth, or recapture rate amongst initial Senegal sole sizes. Around 80% of recaptures were registered within 15 weeks after release. The results show that it is possible for the release and recovery of tagged Senegal soles in the Gulf of Cadiz. Future long-term programmes on stock enhancement could help to determine the effects on fisheries and recover stocks.
    Keywords Dicologlossa cuneata ; Scophthalmus rhombus ; Solea senegalensis ; coasts ; fish ; Senegal
    Language English
    Dates of publication 2023-01
    Size p. 203-214.
    Publishing place Springer International Publishing
    Document type Article ; Online
    ZDB-ID 214428-1
    ISSN 1573-5117 ; 0018-8158
    ISSN (online) 1573-5117
    ISSN 0018-8158
    DOI 10.1007/s10750-022-05054-w
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Bacteria driving droplets.

    Ramos, Gabriel / Cordero, María Luisa / Soto, Rodrigo

    Soft matter

    2020  Volume 16, Issue 5, Page(s) 1359–1365

    Abstract: We confine a dense suspension of motile Escherichia coli inside a spherical droplet in a water-in-oil emulsion, creating a "bacterially" propelled droplet. We show that droplets move in a persistent random walk, with a persistence time τ∼ 0.3 s, a long- ... ...

    Abstract We confine a dense suspension of motile Escherichia coli inside a spherical droplet in a water-in-oil emulsion, creating a "bacterially" propelled droplet. We show that droplets move in a persistent random walk, with a persistence time τ∼ 0.3 s, a long-time diffusion coefficient D∼ 0.5 μm
    MeSH term(s) Biomechanical Phenomena ; Emulsions/chemistry ; Escherichia coli/chemistry ; Escherichia coli/cytology ; Oils/chemistry ; Surface Properties ; Surface-Active Agents/chemistry ; Water/chemistry
    Chemical Substances Emulsions ; Oils ; Surface-Active Agents ; Water (059QF0KO0R)
    Language English
    Publishing date 2020-01-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 2191476-X
    ISSN 1744-6848 ; 1744-683X
    ISSN (online) 1744-6848
    ISSN 1744-683X
    DOI 10.1039/c9sm01839e
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Book ; Online: Local details versus effective medium approximation

    Ponce, Washington / Cordero, Maria Luisa

    A study of diffusion in microfluidic random networks made from Voronoi tessellations

    2019  

    Abstract: We measured the effective diffusion coefficient in regions of microfluidic networks of controlled geometry using the FRAP (Fluorescence Recovery After Photobleaching) technique. The geometry of the networks was based on Voronoi tessellations, and had ... ...

    Abstract We measured the effective diffusion coefficient in regions of microfluidic networks of controlled geometry using the FRAP (Fluorescence Recovery After Photobleaching) technique. The geometry of the networks was based on Voronoi tessellations, and had varying characteristic length scale and porosity. For a fixed network, FRAP experiments were performed in regions of increasing size. Our results indicate that the boundary of the bleached region, and in particular the cumulative area of the channels that connect the bleached region to the rest of the network, are important in the measured value of the effective diffusion coefficient. We found that the statistical geometrical variations between different regions of the network decrease with the size of the bleached region as a power law, meaning that the statistical error of effective medium approximations decrease with the size of the studied medium, although no characteristic length scale could be defined over which the porous medium is equivalent to an effective medium.
    Keywords Physics - Biological Physics ; Condensed Matter - Soft Condensed Matter
    Subject code 612
    Publishing date 2019-03-18
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Book ; Online: Bacteria driving droplets

    Ramos, Gabriel / Cordero, Maria Luisa / Soto, Rodrigo

    2019  

    Abstract: We confine a dense suspension of motile \textit{Escherichia coli} inside a spherical droplet in a water-in-oil emulsion, creating a "bacterially" propelled droplet. We show that droplets move in a persistent random walk, with a persistence time $\tau\sim ...

    Abstract We confine a dense suspension of motile \textit{Escherichia coli} inside a spherical droplet in a water-in-oil emulsion, creating a "bacterially" propelled droplet. We show that droplets move in a persistent random walk, with a persistence time $\tau\sim 0.3\, {\rm s}$, a long-time diffusion coefficient $D\sim 0.5\, \mu {\rm m}^2/{\rm s}$, and an average instantaneous speed $V\sim 1.5\, \mu{\rm m/s}$ when the bacterial suspension is at the maximum studied concentration. Several droplets are analyzed, varying the drop radius and bacterial concentration. We show that the persistence time, diffusion coefficient and average speed increase with the bacterial concentration inside the drop, but are largely independent of the droplet size. By measuring the turbulent-like motion of the bacteria inside the drop, we demonstrate that the mean velocity of the bacteria near the bottom of the drop, which is separated from a glass substrate by a thin lubrication oil film, is antiparallel to the instantaneous velocity of the drop. This suggests that the driving mechanism is a slippery rolling of the drop over the substrate, caused by the collective motion of the bacteria. Our results show that microscopic organisms can transfer useful mechanical energy to their confining environment, opening the way to the assembly of mesoscopic motors composed of microswimmers.
    Keywords Condensed Matter - Soft Condensed Matter ; Physics - Biological Physics
    Subject code 612
    Publishing date 2019-12-09
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Medición del gasto energético de reposo en pacientes oncológicos pediátricos: concordancia entre calorimetría indirecta y ecuaciones predictivas.

    Muñoz Benavides, Eliana / Cordero, María Luisa / Castro, Magdalena / Derado, Macareba

    Nutricion hospitalaria

    2018  Volume 35, Issue 3, Page(s) 538–542

    Abstract: Introduction: in childhood cancer, the disease impacts resting energy expenditure (GER) in a way that is not estimable by predictive equations.: Objective: the aim of this study is to determine the concordance between the measurement of resting ... ...

    Title translation Measurement of rest energy expanditure in pediatric oncological patients: concordance between indirect calorimetry and predictive equations.
    Abstract Introduction: in childhood cancer, the disease impacts resting energy expenditure (GER) in a way that is not estimable by predictive equations.
    Objective: the aim of this study is to determine the concordance between the measurement of resting energy expenditure (REE) by indirect calorimetry in pediatric oncology patients versus the World Health Organization (WHO) and Schofield predictive equations.
    Method: cross-sectional study in children aged 5-15 years receiving chemotherapy, in outpatient Clínica Las Condes and Hospital Dr. Sótero del Río, from July 2013 to July 2015. REE measurement was performed by indirect calorimetry and WHO and Schofield equations. Concordance analysis, with clinically relevant cut-off point and concordance coefficient of 90%.
    Results: twenty-seven children were included and 27 calorimetries were performed; 66% of these children were diagnosed with leukemia, 15% with central nervous system tumor and 81% were in the maintenance stage of their treatment. There is no significant difference between indirect calorimetry measurement versus WHO (p 0.18) or Schofield (p 0.07), neither when stratifying by nutritional status or type of cancer diagnosis. Concordance was calculated between calorimetry and Schofield, with a concordance coefficient of Lin = 79.4% (95% CI = 65.2-93.6) and versus WHO = 78% (95% CI = 62.9-93.2).
    Conclusion: this level of agreement, less than 80% in both cases, is insufficient. With both equations for estimating REE, there is overestimation or underestimation of energy requirements in more than 20% of cases. There is no agreement between the measurement of REE measured with indirect calorimetry versus its estimation with Schofield's and the WHO equations. Consequently, indirect calorimetry is required as part of the nutritional assessment in a nutritionally at-risk population such as pediatric patients with oncological pathology.
    MeSH term(s) Adolescent ; Algorithms ; Calorimetry, Indirect ; Child ; Child, Preschool ; Cross-Sectional Studies ; Energy Metabolism ; Female ; Humans ; Male ; Neoplasms/metabolism ; Nutritional Status ; Rest
    Language Spanish
    Publishing date 2018-06-04
    Publishing country Spain
    Document type Journal Article ; Observational Study
    ZDB-ID 1481223-x
    ISSN 1699-5198 ; 0212-1611
    ISSN (online) 1699-5198
    ISSN 0212-1611
    DOI 10.20960/nh.1457
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Magnetotactic bacteria in a droplet self-assemble into a rotary motor.

    Vincenti, Benoit / Ramos, Gabriel / Cordero, Maria Luisa / Douarche, Carine / Soto, Rodrigo / Clement, Eric

    Nature communications

    2019  Volume 10, Issue 1, Page(s) 5082

    Abstract: From intracellular protein trafficking to large-scale motion of animal groups, the physical concepts driving the self-organization of living systems are still largely unraveled. Self-organization of active entities, leading to novel phases and emergent ... ...

    Abstract From intracellular protein trafficking to large-scale motion of animal groups, the physical concepts driving the self-organization of living systems are still largely unraveled. Self-organization of active entities, leading to novel phases and emergent macroscopic properties, recently shed new light on these complex dynamical processes. Here we show that under the application of a constant magnetic field, motile magnetotactic bacteria confined in water-in-oil droplets self-assemble into a rotary motor exerting a torque on the external oil phase. A collective motion in the form of a large-scale vortex, reversable by inverting the field direction, builds up in the droplet with a vorticity perpendicular to the magnetic field. We study this collective organization at different concentrations, magnetic fields and droplet radii and reveal the formation of two torque-generating areas close to the droplet interface. We characterize quantitatively the mechanical energy extractable from this new biological and self-assembled motor.
    MeSH term(s) Alkanes ; Emulsions ; Hydrodynamics ; Magnetic Fields ; Magnetospirillum/physiology ; Oils ; Rotation ; Taxis Response/physiology ; Torque ; Water
    Chemical Substances Alkanes ; Emulsions ; Oils ; Water (059QF0KO0R) ; n-hexadecane (F8Z00SHP6Q)
    Language English
    Publishing date 2019-11-08
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-019-13031-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Effect of confinement on the deformation of microfluidic drops.

    Ulloa, Camilo / Ahumada, Alberto / Cordero, María Luisa

    Physical review. E, Statistical, nonlinear, and soft matter physics

    2014  Volume 89, Issue 3, Page(s) 33004

    Abstract: We study the deformation of drops squeezed between the floor and ceiling of a microchannel and subjected to a hyperbolic flow. We observe that the maximum deformation of drops depends on both the drop size and the rate of strain of the external flow and ... ...

    Abstract We study the deformation of drops squeezed between the floor and ceiling of a microchannel and subjected to a hyperbolic flow. We observe that the maximum deformation of drops depends on both the drop size and the rate of strain of the external flow and can be described with power laws with exponents 2.59±0.28 and 0.91±0.05, respectively. We develop a theoretical model to describe the deformation of squeezed drops based on the Darcy approximation for shallow geometries and the use of complex potentials. The model describes the steady-state deformation of the drops as a function of a nondimensional parameter Caδ2, where Ca is the capillary number (proportional to the strain rate and the drop size) and δ is a confinement parameter equal to the drop size divided by the channel height. For small deformations, the theoretical model predicts a linear relationship between the deformation of drops and this parameter, in good agreement with the experimental observations.
    Language English
    Publishing date 2014-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1550-2376
    ISSN (online) 1550-2376
    DOI 10.1103/PhysRevE.89.033004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Study of the interaction of folic acid-modified gold nanorods and fibrinogen through microfluidics: implications for protein adsorption, incorporation and viability of cancer cells.

    Orellana, Nacaroha / Palma, Sujey / Torres, Estefania / Cordero, María Luisa / Vio, Valentina / Ruso, Juan M / Juárez, Josué / Topete, Antonio / Araya, Eyleen / Vasquez-Contreras, Rodrigo / Kogan, Marcelo J / Hassan, Natalia

    Nanoscale

    2021  Volume 13, Issue 42, Page(s) 17807–17821

    Abstract: Gold nanoparticles (GNPs) are an attractive nanomaterial for potential applications in therapy and diagnostics due to their capability to direct toward specific sites in the organism. However, when exposed to plasma, GNPs can interact with different ... ...

    Abstract Gold nanoparticles (GNPs) are an attractive nanomaterial for potential applications in therapy and diagnostics due to their capability to direct toward specific sites in the organism. However, when exposed to plasma, GNPs can interact with different biomolecules that form a dynamic nano-bio interface called a "protein corona" (PC). Remarkably, the PC could affect multiple biological processes, such as cell targeting and uptake, cytotoxicity, and nanoparticle (NP) clearance. The interaction of nanomaterials with plasmatic proteins has been widely studied under bulk conditions, however, under dynamic conditions, it has just recently been explored. Thus, to mimic a dynamic natural environment found in arteries and veins, microfluidic devices were used. In this work, gold nanorods (GNRs) were synthesized and conjugated with polyethylene glycol (PEG) to reduce their interaction with plasma proteins and increase their biocompatibility. Then, GNRs were functionalized with folic acid, a targeting ligand typically used to recognize tumor cells. The resulting nanosystem was exposed to fibrinogen (FB) to study the development and biological impact of PC formation through two strategies: bulk and laminar flow conditions. The obtained nanosystems were characterized by absorption spectrophotometry, DLS, laser Doppler microelectrophoresis, neutron activation analysis, circular dichroism spectroscopy and TEM. Finally, cell viability and cellular uptake assays were performed to study the influence of the PC on the cell viability and delivery of nanosystems.
    MeSH term(s) Adsorption ; Fibrinogen ; Folic Acid ; Gold ; Metal Nanoparticles/toxicity ; Microfluidics ; Nanotubes ; Neoplasms/drug therapy ; Polyethylene Glycols
    Chemical Substances Polyethylene Glycols (3WJQ0SDW1A) ; Gold (7440-57-5) ; Fibrinogen (9001-32-5) ; Folic Acid (935E97BOY8)
    Language English
    Publishing date 2021-11-04
    Publishing country England
    Document type Journal Article
    ZDB-ID 2515664-0
    ISSN 2040-3372 ; 2040-3364
    ISSN (online) 2040-3372
    ISSN 2040-3364
    DOI 10.1039/d1nr03179a
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Book ; Online: Magnetotactic bacteria in a droplet self-assemble into a rotary motor

    Vincenti, Benoit / Ramos, Gabriel / Cordero, Maria Luisa / Douarche, Carine / Soto, Rodrigo / Clément, Eric

    2019  

    Abstract: From intracellular protein trafficking to large scale motion of animal groups, the physical concepts driving the self-organization of living systems are still largely unraveled. Selforganization of active entities, leading to novel phases and emergent ... ...

    Abstract From intracellular protein trafficking to large scale motion of animal groups, the physical concepts driving the self-organization of living systems are still largely unraveled. Selforganization of active entities, leading to novel phases and emergent macroscopic properties, recently shed new lights on these complex dynamical processes. Here we show that, under the application of a constant magnetic field, motile magnetotactic bacteria confined in water-in-oil droplets self-assemble into a rotary motor exerting a torque on the external oil phase. A collective motion in the form of a large-scale vortex, reversable by inverting the field direction, builds-up in the droplet with a vorticity perpendicular to the magnetic field. We study this collective organization at different concentrations, magnetic fields and droplets radii and reveal the formation of two torque-generating areas close to the droplet interface. We characterize quantitatively the mechanical energy extractable from this new biological and self-assembled motor.
    Keywords Condensed Matter - Soft Condensed Matter
    Subject code 612
    Publishing date 2019-03-03
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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