Article ; Online: Syntaxin 11 Contributes to the Interferon-Inducible Restriction of Coxiella burnetii Intracellular Infection.
2023 Volume 14, Issue 1, Page(s) e0354522
Abstract: There is a limited understanding of host defense mechanisms targeting intracellular pathogens that proliferate in a lysosome. Coxiella burnetii is a model bacterial pathogen capable of replicating in the hydrolytic and acidic environment of the lysosome. ...
Abstract | There is a limited understanding of host defense mechanisms targeting intracellular pathogens that proliferate in a lysosome. Coxiella burnetii is a model bacterial pathogen capable of replicating in the hydrolytic and acidic environment of the lysosome. It has been shown that gamma interferon (IFNγ)-stimulated host cells restrict C. burnetii replication by a mechanism that involves host IDO1 depletion of tryptophan. Host cells deficient in IDO1 activity, however, retain the ability to restrict C. burnetii replication when stimulated with IFNγ, which suggests additional mechanisms of host defense. This study identified syntaxin 11 (STX11) as a host protein that contributes to IFNγ-mediated suppression of C. burnetii replication. STX11 is a SNARE protein; SNARE proteins are proteins that mediate fusion of host vesicles with specific subcellular organelles. Depletion of STX11 using either small interfering RNA (siRNA)- or CRISPR-based approaches enhanced C. burnetii replication intracellularly. Stable expression of STX11 reduced C. burnetii replication in epithelial cells and macrophages, which indicates that this STX11-dependent cell-autonomous response is operational in multiple cell types and can function independently of other IFNγ-induced factors. Fluorescently tagged STX11 localized to the |
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MeSH term(s) | Humans ; Coxiella burnetii ; Host-Pathogen Interactions/physiology ; Interferon-gamma/metabolism ; Interferons/metabolism ; Q Fever/metabolism ; Qa-SNARE Proteins/genetics ; Qa-SNARE Proteins/metabolism ; RNA, Small Interfering/metabolism ; Vacuoles/metabolism |
Chemical Substances | Interferon-gamma (82115-62-6) ; Interferons (9008-11-1) ; Qa-SNARE Proteins ; RNA, Small Interfering ; STX11 protein, human |
Language | English |
Publishing date | 2023-02-02 |
Publishing country | United States |
Document type | Journal Article ; Research Support, N.I.H., Extramural |
ZDB-ID | 2557172-2 |
ISSN | 2150-7511 ; 2161-2129 |
ISSN (online) | 2150-7511 |
ISSN | 2161-2129 |
DOI | 10.1128/mbio.03545-22 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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