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  1. Article ; Online: Protein antibiotics: mind your language.

    Correia, Annapaula / Weimann, Aaron

    Nature reviews. Microbiology

    2020  Volume 19, Issue 1, Page(s) 7

    MeSH term(s) Anti-Bacterial Agents/chemistry ; Anti-Bacterial Agents/pharmacology ; Antimicrobial Cationic Peptides/genetics ; Antimicrobial Cationic Peptides/pharmacology ; Bacterial Proteins/chemistry ; Bacterial Proteins/genetics ; Bacterial Proteins/pharmacology ; Data Mining ; Drug Discovery/methods ; Genomics/methods ; Humans ; Proteins/chemistry ; Proteins/genetics ; Proteins/pharmacology
    Chemical Substances Anti-Bacterial Agents ; Antimicrobial Cationic Peptides ; Bacterial Proteins ; Proteins
    Language English
    Publishing date 2020-11-11
    Publishing country England
    Document type Letter
    ZDB-ID 2139054-X
    ISSN 1740-1534 ; 1740-1526
    ISSN (online) 1740-1534
    ISSN 1740-1526
    DOI 10.1038/s41579-020-00485-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  2. Article ; Online: Ribosomal MLST nucleotide identity (rMLST-NI), a rapid bacterial species identification method: application to

    Bray, James E / Correia, Annapaula / Varga, Margaret / Jolley, Keith A / Maiden, Martin C J / Rodrigues, Charlene M C

    Microbial genomics

    2022  Volume 8, Issue 9

    Abstract: Bacterial genomics is making an increasing contribution to the fields of medicine and public health microbiology. Consequently, accurate species identification of bacterial genomes is an important task, particularly as the number of genomes stored in ... ...

    Abstract Bacterial genomics is making an increasing contribution to the fields of medicine and public health microbiology. Consequently, accurate species identification of bacterial genomes is an important task, particularly as the number of genomes stored in online databases increases rapidly and new species are frequently discovered. Existing database entries require regular re-evaluation to ensure that species annotations are consistent with the latest species definitions. We have developed an automated method for bacterial species identification that is an extension of ribosomal multilocus sequence typing (rMLST). The method calculates an 'rMLST nucleotide identity' (rMLST-NI) based on the nucleotides present in the protein-encoding ribosomal genes derived from bacterial genomes. rMLST-NI was used to validate the species annotations of 11839 publicly available
    MeSH term(s) Genome, Bacterial/genetics ; Genomics/methods ; Klebsiella/genetics ; Multilocus Sequence Typing/methods ; Nucleotides
    Chemical Substances Nucleotides
    Language English
    Publishing date 2022-09-12
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2835258-0
    ISSN 2057-5858 ; 2057-5858
    ISSN (online) 2057-5858
    ISSN 2057-5858
    DOI 10.1099/mgen.0.000849
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Engineering bacteriocin-mediated resistance against the plant pathogen Pseudomonas syringae.

    Rooney, William M / Grinter, Rhys W / Correia, Annapaula / Parkhill, Julian / Walker, Daniel C / Milner, Joel J

    Plant biotechnology journal

    2019  Volume 18, Issue 5, Page(s) 1296–1306

    Abstract: The plant pathogen, Pseudomonas syringae (Ps), together with related Ps species, infects and attacks a wide range of agronomically important crops, including tomato, kiwifruit, pepper, olive and soybean, causing economic losses. Currently, chemicals and ... ...

    Abstract The plant pathogen, Pseudomonas syringae (Ps), together with related Ps species, infects and attacks a wide range of agronomically important crops, including tomato, kiwifruit, pepper, olive and soybean, causing economic losses. Currently, chemicals and introduced resistance genes are used to protect plants against these pathogens but have limited success and may have adverse environmental impacts. Consequently, there is a pressing need to develop alternative strategies to combat bacterial disease in crops. One such strategy involves using narrow-spectrum protein antibiotics (so-called bacteriocins), which diverse bacteria use to compete against closely related species. Here, we demonstrate that one bacteriocin, putidacin L1 (PL1), can be expressed in an active form at high levels in Arabidopsis and in Nicotiana benthamiana in planta to provide effective resistance against diverse pathovars of Ps. Furthermore, we find that Ps strains that mutate to acquire tolerance to PL1 lose their O-antigen, exhibit reduced motility and still cannot induce disease symptoms in PL1-transgenic Arabidopsis. Our results provide proof-of-principle that the transgene-mediated expression of a bacteriocin in planta can provide effective disease resistance to bacterial pathogens. Thus, the expression of bacteriocins in crops might offer an effective strategy for managing bacterial disease, in the same way that the genetic modification of crops to express insecticidal proteins has proven to be an extremely successful strategy for pest management. Crucially, nearly all genera of bacteria, including many plant pathogenic species, produce bacteriocins, providing an extensive source of these antimicrobial agents.
    MeSH term(s) Bacteriocins/genetics ; Disease Resistance/genetics ; Humans ; Lycopersicon esculentum ; Plant Diseases ; Pseudomonas syringae
    Chemical Substances Bacteriocins
    Language English
    Publishing date 2019-12-03
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2136367-5
    ISSN 1467-7652 ; 1467-7644
    ISSN (online) 1467-7652
    ISSN 1467-7644
    DOI 10.1111/pbi.13294
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Engineering bacteriocin‐mediated resistance against the plant pathogen Pseudomonas syringae

    Rooney, William M / Grinter, Rhys W / Correia, Annapaula / Parkhill, Julian / Walker, Daniel C / Milner, Joel J

    Plant biotechnology journal. 2020 May, v. 18, no. 5

    2020  

    Abstract: The plant pathogen, Pseudomonas syringae (Ps), together with related Ps species, infects and attacks a wide range of agronomically important crops, including tomato, kiwifruit, pepper, olive and soybean, causing economic losses. Currently, chemicals and ... ...

    Abstract The plant pathogen, Pseudomonas syringae (Ps), together with related Ps species, infects and attacks a wide range of agronomically important crops, including tomato, kiwifruit, pepper, olive and soybean, causing economic losses. Currently, chemicals and introduced resistance genes are used to protect plants against these pathogens but have limited success and may have adverse environmental impacts. Consequently, there is a pressing need to develop alternative strategies to combat bacterial disease in crops. One such strategy involves using narrow‐spectrum protein antibiotics (so‐called bacteriocins), which diverse bacteria use to compete against closely related species. Here, we demonstrate that one bacteriocin, putidacin L1 (PL1), can be expressed in an active form at high levels in Arabidopsis and in Nicotiana benthamiana in planta to provide effective resistance against diverse pathovars of Ps. Furthermore, we find that Ps strains that mutate to acquire tolerance to PL1 lose their O‐antigen, exhibit reduced motility and still cannot induce disease symptoms in PL1‐transgenic Arabidopsis. Our results provide proof‐of‐principle that the transgene‐mediated expression of a bacteriocin in planta can provide effective disease resistance to bacterial pathogens. Thus, the expression of bacteriocins in crops might offer an effective strategy for managing bacterial disease, in the same way that the genetic modification of crops to express insecticidal proteins has proven to be an extremely successful strategy for pest management. Crucially, nearly all genera of bacteria, including many plant pathogenic species, produce bacteriocins, providing an extensive source of these antimicrobial agents.
    Keywords Arabidopsis ; Nicotiana benthamiana ; O-antigens ; Pseudomonas syringae ; bacteriocins ; biotechnology ; disease resistance ; genetic engineering ; kiwifruit ; olives ; pathovars ; pepper ; pest management ; plant pathogens ; soybeans ; tomatoes
    Language English
    Dates of publication 2020-05
    Size p. 1296-1306.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 2136367-5
    ISSN 1467-7652 ; 1467-7644
    ISSN (online) 1467-7652
    ISSN 1467-7644
    DOI 10.1111/pbi.13294
    Database NAL-Catalogue (AGRICOLA)

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  5. Article: Photobactericidal polymers; the incorporation of crystal violet and nanogold into medical grade silicone

    Noimark, Sacha / Allan, Elaine / Bovis, Melissa / Correia, Annapaula / MacRobert, Alexander J / Parkin, Ivan P / Wilson, Michael

    RSC advances. 2013 Sept. 27, v. 3, no. 40

    2013  

    Abstract: Crystal violet and 2 nm gold nanoparticles were incorporated into medical grade silicone polymers by use of a novel two-step dipping strategy using water and water–acetone mixtures. Optical microscopic examination showed that the optimised polymer ... ...

    Abstract Crystal violet and 2 nm gold nanoparticles were incorporated into medical grade silicone polymers by use of a novel two-step dipping strategy using water and water–acetone mixtures. Optical microscopic examination showed that the optimised polymer incorporated dye close to the polymer surface, with minimal dye encapsulation throughout the polymer bulk. The modified polymer was stable under aqueous conditions with negligible leaching of crystal violet from the polymer into surrounding aqueous solution at 37 °C. Exposure of the modified silicone to low power 635 nm laser light induced the lethal photosensitisation of both Staphylococcus epidermidis and Escherichia coli. Despite the laser used not matching the absorption maximum of the crystal violet-containing silicone samples, the lethal photosensitisation was the highest reported, in terms of bacterial kill per energy dose. Furthermore, surprisingly, some statistically significant dark kill was also noted.
    Keywords absorption ; antibacterial properties ; aqueous solutions ; dipping ; encapsulation ; energy ; Escherichia coli ; gentian violet ; leaching ; microscopy ; nanogold ; photosensitivity ; silicone ; Staphylococcus epidermidis
    Language English
    Dates of publication 2013-0927
    Size p. 18383-18394.
    Publishing place The Royal Society of Chemistry
    Document type Article
    ISSN 2046-2069
    DOI 10.1039/c3ra42629g
    Database NAL-Catalogue (AGRICOLA)

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