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  1. Article ; Online: Mortality in cutaneous malignant melanoma and its association with Neutrophil-to-Lymphocyte ratio.

    Pinto-Paz, Mirian Elizabeth / Cotrina-Concha, Jose Manuel / Benites-Zapata, Vicente A

    Cancer treatment and research communications

    2021  Volume 29, Page(s) 100464

    Abstract: Introduction: Cutaneous malignant melanoma (CMM) incidence has risen rapidly in the last 50 years. Poor progression and high mortality characterize CMM, making a thorough understanding of progression and associated factors essential for optimizing care.! ...

    Abstract Introduction: Cutaneous malignant melanoma (CMM) incidence has risen rapidly in the last 50 years. Poor progression and high mortality characterize CMM, making a thorough understanding of progression and associated factors essential for optimizing care.
    Aims: We assessed the association between the Neutrophil-to-Lymphocyte Ratio (NLR) and mortality in adults with CMM from an entirely mixed-race Hispanic population during 12 consecutive years of extensive follow-up.
    Material & methods: We performed a retrospective cohort study in a tertiary hospital in Peru. NLR was categorized with a cutoff value higher or equal than 3. We collected demographic variables, laboratory results and treatments at baseline of follow-up. Cox regression analysis was performed, and we calculated crude and adjusted hazard ratios (HR) and their 95% confidence interval (95%CI).
    Results: The analysis was from 615 CMM cases, and there were 378 deaths. Most melanomas (63.6%) were acral lentiginous. The crude analysis showed that high NLR is a risk factor for mortality, HR = 2.52; 95%CI (2.03-3.14). High NRL ratio remains statistically significant after adjusting for confounding variables, aHR = 1.61; 95%CI (1.16-2.24). Other risk factors for mortality were clinical stages III and IV, older than 60 years, females and greater Breslow thickness.
    Conclusions: We concluded that high NRL ratio is a risk factor for mortality and should be monitored in every patient who is diagnosed with malignant melanoma during their first blood count. It should then be carried out in follow-up controls for patients of clinical stage III and IV only, or in patients who present a relapse.
    MeSH term(s) Cohort Studies ; Disease Progression ; Female ; Humans ; Lymphocytes/metabolism ; Male ; Melanoma/blood ; Melanoma/mortality ; Neutrophils/metabolism ; Retrospective Studies ; Skin Neoplasms/blood ; Skin Neoplasms/mortality ; Survival Analysis ; Melanoma, Cutaneous Malignant
    Language English
    Publishing date 2021-09-25
    Publishing country England
    Document type Journal Article
    ISSN 2468-2942
    ISSN (online) 2468-2942
    DOI 10.1016/j.ctarc.2021.100464
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Chip-based digital Polymerase Chain Reaction as quantitative technique for the detection of PIK3CA mutations in breast cancer patients

    Giannoni-Luza, Stefano / Acosta, Óscar / Murillo Carrasco, Alexis Germán / Danos, Pierina / Cotrina Concha, José Manuel / Guerra Miller, Henry / Pinto, Joseph A. / Aguilar, Alfredo / Araujo, Jhajaira M. / Fujita, Ricardo / Buleje, Jose

    Heliyon. 2022 Nov., v. 8, no. 11 p.e11396-

    2022  

    Abstract: PIK3CA is a gene frequently mutated in breast cancer. With the FDA approval of alpelisib, the evaluation of PIK3CA for activating mutations is becoming routinely. Novel platforms for gene analysis as digital PCR (dPCR) are emerging as a potential ... ...

    Abstract PIK3CA is a gene frequently mutated in breast cancer. With the FDA approval of alpelisib, the evaluation of PIK3CA for activating mutations is becoming routinely. Novel platforms for gene analysis as digital PCR (dPCR) are emerging as a potential replacement for the traditional Sanger sequencing. However, there are still few studies on chip-based dPCR to detect mutations in tumor samples. Thus, this cross-sectional study aimed to assess the sensibility of a chip-based dPCR to detect and quantify PIK3CA mutations and compare its performance with Sanger sequencing. Tumor samples from 57 breast cancer patients (22 pre-treatment samples, 32 tumors after neoadjuvant chemotherapy, and three lymph nodes) were collected and analyzed by Sanger sequencing and dPCR for the three PIK3CA most relevant mutations (p.E545K, p. H1047R, and p. H1047L). Digital PCR sensitivity, specificity, and overall performance were estimated by contingency tables, receptor operator characteristic (ROC), and area under the curve (AUC). Association of PIK3CA mutations with clinicopathological variables was conducted. Sanger sequencing identified PIK3CA mutations in six patients (10.5%), two with p. H1047R, and four with p. E545K. Digital PCR confirmed those mutations and identified 19 additional patients with at least one mutation. Comparison between dPCR and Sanger sequencing showed a sensitivity of 100% (95% CI 53–100%), and a specificity of 84.2% (95% CI 83–84.2%). Besides, p. H1047R mutation detected by dPCR showed a significant association with breast cancer phenotype (p = 0.019) and lymphatic nodes infiltration (p = 0.046). Digital PCR showed a high sensitivity to detect mutations in tumor samples and it might be capable to detect low-rate mutations and tumor subpopulations not detected by Sanger sequencing.
    Keywords breast neoplasms ; cross-sectional studies ; drug therapy ; genes ; lymph ; mutation ; phenotype ; polymerase chain reaction ; quantitative analysis ; PIK3CA ; Genetic techniques ; Digital PCR
    Language English
    Dates of publication 2022-11
    Publishing place Elsevier Ltd
    Document type Article ; Online
    Note Use and reproduction
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2022.e11396
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Promoter hypermethylation of RARB and GSTP1 genes in plasma cell-free DNA as breast cancer biomarkers in Peruvian women.

    Danos, Pierina / Giannoni-Luza, Stefano / Murillo Carrasco, Alexis Germán / Acosta, Oscar / Guevara-Fujita, Maria Luisa / Cotrina Concha, José Manuel / Guerra Miller, Henry / Pinto Oblitas, Joseph / Aguilar Cartagena, Alfredo / Araujo, Jhajaira M / Fujita, Ricardo / Buleje Sono, José Luis

    Molecular genetics & genomic medicine

    2023  Volume 11, Issue 12, Page(s) e2260

    Abstract: Background: Promoter hypermethylation is one of the enabling mechanisms of hallmarks of cancer. Tumor suppressor genes like RARB and GSTP1 have been reported as hypermethylated in breast cancer tumors compared with normal tissues in several populations. ...

    Abstract Background: Promoter hypermethylation is one of the enabling mechanisms of hallmarks of cancer. Tumor suppressor genes like RARB and GSTP1 have been reported as hypermethylated in breast cancer tumors compared with normal tissues in several populations. This case-control study aimed to determine the association between the promoter methylation ratio (PMR) of RARB and GSTP1 genes (separately and as a group) with breast cancer and its clinical-pathological variables in Peruvian patients, using a liquid biopsy approach.
    Methods: A total of 58 breast cancer patients and 58 healthy controls, matched by age, participated in the study. We exacted cell-free DNA (cfDNA) from blood plasma and converted it by bisulfite salts. Methylight PCR was performed to obtain the PMR value of the studied genes. We determined the association between PMR and breast cancer, in addition to other clinicopathological variables. The sensitivity and specificity of the PMR of these genes were obtained.
    Results: A significant association was not found between breast cancer and the RARB PMR (OR = 1.90; 95% CI [0.62-6.18]; p = 0.210) or the GSTP1 PMR (OR = 6.57; 95% CI [0.75-307.66]; p = 0.114). The combination of the RARB + GSTP1 PMR was associated with breast cancer (OR = 2.81; 95% CI [1.02-8.22]; p = 0.026), controls under 50 years old (p = 0.048), patients older than 50 (p = 0.007), and postmenopausal (p = 0.034). The PMR of both genes showed a specificity of 86.21% and a sensitivity of 31.03%.
    Conclusion: Promoter hypermethylation of RARB + GSTP1 genes is associated with breast cancer, older age, and postmenopausal Peruvian patients. The methylated promoter of the RARB + GSTP1 genes needs further validation to be used as a biomarker for liquid biopsy and as a recommendation criterion for additional tests in asymptomatic women younger than 50 years.
    MeSH term(s) Female ; Humans ; Middle Aged ; Biomarkers, Tumor/genetics ; Breast/pathology ; Breast Neoplasms/genetics ; Breast Neoplasms/pathology ; Case-Control Studies ; DNA Methylation ; Glutathione S-Transferase pi/genetics ; Peru
    Chemical Substances Biomarkers, Tumor ; Glutathione S-Transferase pi (EC 2.5.1.18) ; GSTP1 protein, human (EC 2.5.1.18) ; retinoic acid receptor beta
    Language English
    Publishing date 2023-08-07
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2734884-2
    ISSN 2324-9269 ; 2324-9269
    ISSN (online) 2324-9269
    ISSN 2324-9269
    DOI 10.1002/mgg3.2260
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Chip-based digital Polymerase Chain Reaction as quantitative technique for the detection of

    Giannoni-Luza, Stefano / Acosta, Oscar / Murillo Carrasco, Alexis Germán / Danos, Pierina / Cotrina Concha, José Manuel / Guerra Miller, Henry / Pinto, Joseph A / Aguilar, Alfredo / Araujo, Jhajaira M / Fujita, Ricardo / Buleje, Jose

    Heliyon

    2022  Volume 8, Issue 11, Page(s) e11396

    Abstract: Background: PIK3CA: Materials and methods: Tumor samples from 57 breast cancer patients (22 pre-treatment samples, 32 tumors after neoadjuvant chemotherapy, and three lymph nodes) were collected and analyzed by Sanger sequencing and dPCR for the ... ...

    Abstract Background: PIK3CA
    Materials and methods: Tumor samples from 57 breast cancer patients (22 pre-treatment samples, 32 tumors after neoadjuvant chemotherapy, and three lymph nodes) were collected and analyzed by Sanger sequencing and dPCR for the three
    Results: Sanger sequencing identified
    Conclusions: Digital PCR showed a high sensitivity to detect mutations in tumor samples and it might be capable to detect low-rate mutations and tumor subpopulations not detected by Sanger sequencing.
    Language English
    Publishing date 2022-11-03
    Publishing country England
    Document type Journal Article
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2022.e11396
    Database MEDical Literature Analysis and Retrieval System OnLINE

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